Difference between revisions of "Team:LZU-China/Parts"

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{{LZU-China}}
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{{Template:LZU_China/Playground/style}}
 
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  <title>Team:LZU 2015</title>
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  <link rel="shortcut icon" href="http://7xkk8b.com1.z0.glb.clouddn.com/imgfavicon.ico" />
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<h2> Part Documentation</h2>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
  
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<h4>Note</h4>
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  <link href="https://2015.igem.org/Team:LZU-China/chz_materialize.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet" media="screen,projection"/>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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  <link href="https://2015.igem.org/Team:LZU-China/chz_style2.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet" media="screen,projection"/>
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      <a id="logo-container" href="#" class="brand-logo"><img src="https://static.igem.org/mediawiki/2015/2/2c/Lzu_china_2015_Igem_icon.png" alt="" class="circle responsive-img avat"></a>
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      <ul class="right">
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/chz_index">Main</a>
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        </li>
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/project">Project</a>
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        </li>
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        <li>
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          <a href="#">Parts</a>
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        </li>
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      <li>
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          <a href="https://2015.igem.org/Team:LZU-China/Practices">Human Practices</a>
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        </li>
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/Attributions">Attributions</a>
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        </li>
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/safety">Safety</a>
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        </li>
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/notes">Notes</a>
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        </li>
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        <li>
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          <a href="https://2015.igem.org/Team:LZU-China/Collaborations">Collaboration</a>
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        </li>
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      </ul>
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      <ul id="nav-mobile" class="side-nav">
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        <li><a href="#">Navbar Link</a></li>
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      <a href="#" data-activates="nav-mobile" class="button-collapse"><i class="mdi-navigation-menu"></i></a>
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    </div>
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</header>
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  <div id="index-banner" class="parallax-container inorder">
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    <div class="section no-pad-bot">
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      <div class="container">
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        <br><br>
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        <h1 class="header center teal-text text-lighten-2"> <span class="cyan-text text-accent-2">Part list</span></h1>
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        <div class="row center">
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        </div>
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        <br><br>
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      </div>
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    </div>
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    <div class="parallax"><img class="bgimagex"  src="https://static.igem.org/mediawiki/2015/8/80/LZU_China_part_banner.png" alt="Unsplashed background img 2"></div>
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  </div>
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<div clsss="row">
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<div class="container">
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<div>
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  <strong><h6><p>RibB is our basic gene. Every part basic on it.</p></h6></strong>
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  <div class="row">
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            <p> <div class="col s12 m5 l5">
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                <div class="card-image">
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                <img class="left-align" src="https://static.igem.org/mediawiki/parts/5/5f/Constitutive.png">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 1:Constitutive parts <br/>K1755005 K1755006 K1755007 K1755008 and K1755009</p>
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                </div>
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                  <a href="#">This is a link</a>
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                  <img class="right-align" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/IGEM_2015_MFC.JPG/600px-IGEM_2015_MFC.JPG">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 2:Our parts working in MFC</p>
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                </div>
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                  <a href="#">This is a link</a>
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              </div></p>
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          </div>
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  <p>
 +
    The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
 +
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
 +
Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB).
 +
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.
 +
  </p>
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  <div class="row">
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            <p> <div class="col s12 m5 l5">
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                <div class="card">
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                <div class="card-image">
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                <img class="left-align" src="https://static.igem.org/mediawiki/parts/0/04/K1755301ceshi.png">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 3:Concentration of riboflavin vs Cu ions</p>
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                </div>
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                  <a href="#">This is a link</a>
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                <div class="card-image">
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                  <img class="right-align" src="https://static.igem.org/mediawiki/parts/b/b5/K1755301time.png">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 4:Riboflavin concentration when the CuSO<sub>4</sub>  in 5mg/L</p>
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                </div>
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        </div></p>
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    </div>
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      <P> We construct many parts as the following list.</P>
 
</div>
 
</div>
  
 +
<div class="col s12">
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    <div class="col s12 m6 l6">
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<table class="responsive-table striped">
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                <thead>
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                    <th><h4>Parts list</h4> </th>
 +
                    </thead>
 +
                <tbody>
 +
                    <tr>
 +
                    <td>Part ID</td>
 +
                    <td>Characterization</td>
 +
                    <td>Details</td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755001</td>
 +
                    <td>ribB coding sequence</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755001">more information</a>
 +
                    </tr>
 +
 +
                    <tr>
 +
                    <td>K1755002</td>
 +
                    <td>ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755002">more information</a></td>
 +
                    </tr>
  
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<h4>Adding parts to the registry</h4>
+
                    <tr>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
                    <td>K1755003</td>
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
                    <td>B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755003">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755004</td>
 +
                    <td>K525998(PT7+RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755004">more information</a></td>
 +
                    </tr>
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
+
                    <tr>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
                    <td>K1755005</td>
−
<ul>
+
                    <td>K608003(strong promoter+medium RBS) + ribB + B0014(terminator)</td>
−
<li>Part Name</li>
+
                    <td><a href="http://parts.igem.org/Part:BBa_K1755005">more information</a></td>
−
<li>Part type</li>
+
                    </tr>
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
                    <tr>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
                    <td>K1755006</td>
 +
                    <td>K608006(medium promoter+medium RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755006">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755007</td>
 +
                    <td>B608002(strong promoter+strong RBS)+ ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755007">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755008</td>
 +
                    <td>K608004(strong  Promoter and weak RBS)+ribB+B0014(Double terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755008">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755009</td>
 +
                    <td>K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755009">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755301</td>
 +
                    <td>MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755301">more information</a></td>
 +
                    </tr>
  
 +
                    <tr>
 +
                    <td>K1755302</td>
 +
                    <td>K190024(Copper sensor) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755302">more information</a></td>
 +
                    </tr>
  
−
<h4>Inspiration</h4>
+
                    <tr>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
                    <td>K1755303</td>
 +
                    <td>K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755303">more information</a></td>
 +
                    </tr>
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
                    <tr>
−
<ul>
+
                    <td>K1755305</td>
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
                    <td>K346002(MerO) + B0032(RBS) + ribB + B0014(terminator)</td>
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
                    <td><a href="http://parts.igem.org/Part:BBa_K1755305">more information</a></td>
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
                    </tr>
−
</ul>
+
  
 +
                    <tr>
 +
                    <td>K1755024</td>
 +
                    <td>K911003+ B0032(RBS) + ribB + B0014(terminator)</td>
 +
                    <td><a href="http://parts.igem.org/Part:BBa_K1755024">more information</a></td>
 +
                    </tr>
  
 +
                   
  
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<h4>Part Table </h4>
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</html>
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                    </tbody>
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<groupparts>iGEM015 Example</groupparts>
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          <h5 class="white-text">LZU-China 2015</h5>
 +
          <p class="grey-text text-lighten-4">NO.222, TianShui South Road, Lanzhou, China, 730000</p>
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          <h6 class="white-text">Copyright 2015 Lanzhou University</h6>
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          <h5 class="white-text">Acknowledgments</h5>
 +
          <ul>
 +
            <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li>
 +
            <li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li>
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            <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of Life Sciences,LZU</a></li>
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Latest revision as of 21:36, 18 September 2015

Team:LZU 2015



Part list



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RibB is our basic gene. Every part basic on it.

Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009

Figure 2:Our parts working in MFC

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.

Figure 3:Concentration of riboflavin vs Cu ions

Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L

We construct many parts as the following list.

Parts list
Part ID Characterization Details
K1755001 ribB coding sequence more information
K1755002 ribB + B0014(terminator) more information
K1755003 B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) more information
K1755004 K525998(PT7+RBS) + ribB + B0014(terminator) more information
K1755005 K608003(strong promoter+medium RBS) + ribB + B0014(terminator) more information
K1755006 K608006(medium promoter+medium RBS) + ribB + B0014(terminator) more information
K1755007 B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) more information
K1755008 K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755009 K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755301 MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) more information
K1755302 K190024(Copper sensor) + ribB + B0014(terminator) more information
K1755303 K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) more information
K1755305 K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) more information
K1755024 K911003+ B0032(RBS) + ribB + B0014(terminator) more information