Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"

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<h3> - Just Kidding...</h3>
 
<h3> - Just Kidding...</h3>
 
<ul class="activiteitlijst">
 
<ul class="activiteitlijst">
−
<li><span class="activiteit">Our first successful Gibsom Assembly turned out to be insuccessful...</span></li>
+
<li><span class="activiteit">Our first successful Gibsom Assembly turned out to be unsuccessful...</span></li>
 
<li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li>
 
<li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li>
 
<li><span class="activiteit">Xbal &amp; pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning</span></li>
 
<li><span class="activiteit">Xbal &amp; pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning</span></li>

Revision as of 14:17, 7 July 2015





Timeline



Week 26

- Preparing for take-off


  • Inventory of the lab supplies
  • Pouring LB Agar plates
  • Amplification of the pET-Duet-1 vector
  • Assessment of the safety requirements
  • Preparing stocks for antibiotics, glycerol, LB & MilliQ


Week 27

- The Clone Wars


  • Linearizing pET-Duet-1 for Gibson Assembly
  • Our first successful Gibson Assembly!
  • Amplification of the pET-Duet-1 vector
  • Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning


Week 28

- Just Kidding...

  • Our first successful Gibsom Assembly turned out to be unsuccessful...
  • Amplification of the inserts for traditional cloning
  • Xbal & pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
  • Xba1 & pst1 digestion of the inserts (MCS-1) for traditional cloning
  • Nde1 & kpn1 digestion of the inserts (MCS-2) for traditional cloning
  • Ligation