Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"
| Line 39: | Line 39: | ||
<br /> | <br /> | ||
<h4>Week 28</h4> | <h4>Week 28</h4> | ||
| − | <h3> - </h3> | + | <h3> - Et tu, pET-Duet? </h3> |
<ul class="activiteitlijst"> | <ul class="activiteitlijst"> | ||
<li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li> | <li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li> | ||
Revision as of 14:21, 7 July 2015
Timeline
Week 26
- Preparing for take-off
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27
- The Clone Wars
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first successful Gibson Assembly!
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning
Week 28
- Et tu, pET-Duet?
- Amplification of the inserts for traditional cloning
- Xbal & pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
- Xba1 & pst1 digestion of the inserts (MCS-1) for traditional cloning
- Nde1 & kpn1 digestion of the inserts (MCS-2) for traditional cloning
- Ligation