Difference between revisions of "Team:KU Leuven/Research/Results"
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In the first step, the Chromobacterium violacein CV026 was grown with different concentrations of OHHL. The C. violacein CV026 was added to the mixtures at an OD of 1.11. Our cells were grown for 24 hours in air-lid culture tubes at 30 °C in a shaking incubator (200 rpm). In figure 1 is clearly visible that a violet pigment is produced. </p> | In the first step, the Chromobacterium violacein CV026 was grown with different concentrations of OHHL. The C. violacein CV026 was added to the mixtures at an OD of 1.11. Our cells were grown for 24 hours in air-lid culture tubes at 30 °C in a shaking incubator (200 rpm). In figure 1 is clearly visible that a violet pigment is produced. </p> | ||
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+ | <a class="example-image-link" href="https://static.igem.org/mediawiki/2015/d/d2/KU_Leuven_ResultOHHL1.jpeg" data-lightbox="example-set" data-title="knockout"><img class="example-image" src="https://static.igem.org/mediawiki/2015/d/d2/KU_Leuven_ResultOHHL1.jpeg" width="82.5%" alt="blokkeert" width="45%" height="45%"></a> | ||
+ | <h4><div id=figure2>Figure 1</div>Culture tubes of inoculated Chromobacterium violacein CV026 with different amounts of OHHL.</h4> | ||
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Revision as of 22:06, 18 September 2015
![](https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg)
Results
Leucine detection
The standard curve from 0 to 100 µM did not give a linear relationship. Our working method needs optimisation. Because the enzymes are from other organisms than mentioned in Kugimiya and Fukada (2015), it is possible that the enzymes have another efficiency and as a consequence need to have another ratio (substrates over enzyme). Additionally, we did not have the same equipment as described in the article: we had to manually pipet the luminol solution. This possibly means that the measurements have a delay.
Due to a lack of time, we couldn’t complete the plasmid assembly and therefore, we were not able to proceed the quantification of leucine.
In comparison to HPLC, the chosen method would be less time consuming without the need of specialized equipment.
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be