Difference between revisions of "Team:Evry/Project/SurfaceDisplay"

Line 41: Line 41:
  
 
<p class="text-justify">To establish a proof of concept, our system was tested in vivo on C57BL/6 mice injected with the melanoma cell line B16-OVA expressing the ovalbumin antigen. We also tested the system in vitro on hybridoma B3Z T-cells specific for SIINFEKL. The tumor antigen cloned in our vector was OVA1 corresponding to the sequence QLESIINFEKLTEW, class I (Kb)-restricted peptide epitope of ovalbumin (OVA) plus 3 amino acids around the epitope to allow better digestion by the proteasome. It is presented by the class I MHC molecule H-2Kb (29).</p>
 
<p class="text-justify">To establish a proof of concept, our system was tested in vivo on C57BL/6 mice injected with the melanoma cell line B16-OVA expressing the ovalbumin antigen. We also tested the system in vitro on hybridoma B3Z T-cells specific for SIINFEKL. The tumor antigen cloned in our vector was OVA1 corresponding to the sequence QLESIINFEKLTEW, class I (Kb)-restricted peptide epitope of ovalbumin (OVA) plus 3 amino acids around the epitope to allow better digestion by the proteasome. It is presented by the class I MHC molecule H-2Kb (29).</p>
e
+
 
 
<div class="row">
 
<div class="row">
<div class="col-md-4"><img border="0" width="500" src="https://static.igem.org/mediawiki/2015/a/ab/Sch%C3%A9ma.jpg" alt="" /></div>
+
<div class="col-md-4"><img border="0" class='img-responsive' src="https://static.igem.org/mediawiki/2015/a/ab/Sch%C3%A9ma.jpg" alt="" /></div>
 
<div class="col-md-8">
 
<div class="col-md-8">
 
<p class="text-justify"> (1) Surface Display of tumor antigen OVA1 fused to DEC205 scFv.   
 
<p class="text-justify"> (1) Surface Display of tumor antigen OVA1 fused to DEC205 scFv.   
Line 49: Line 49:
 
    (3) CD8+ T cell cross-priming with tumor antigen OVA1
 
    (3) CD8+ T cell cross-priming with tumor antigen OVA1
 
  (4)  Cancer cell lysis with antigen OVA1 targetin </p>
 
  (4)  Cancer cell lysis with antigen OVA1 targetin </p>
</div>
 
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 22:15, 18 September 2015

Scroll to top To top