Difference between revisions of "Team:Paris Saclay/Notebook/July/7"
(Created page with "=Tuesday 7th July= ==Back in Lab== ====Verification of our plasmid==== ''by Johan'' Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504 ''by...") |
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==Back in Lab== | ==Back in Lab== | ||
====Verification of our plasmid==== | ====Verification of our plasmid==== | ||
| − | ''by Johan'' | + | ''by Coralie, Johan, Seong Koo, Audrey, Pauline'' |
Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504 | Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504 | ||
| − | + | Digestion by NotI : | |
| + | Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI | ||
| + | We use 18uL of this solution with 2uL plasmid solution | ||
| + | Digestion is process during 1h30 at 37°C | ||
====Electrophoresis==== | ====Electrophoresis==== | ||
| − | ''by Johan, | + | ''by Johan, Audrey, Pauline'' |
| + | |||
| + | Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET | ||
| + | Migration 0,06A 80V | ||
====New culture==== | ====New culture==== | ||
Revision as of 15:16, 7 July 2015
Contents
Tuesday 7th July
Back in Lab
Verification of our plasmid
by Coralie, Johan, Seong Koo, Audrey, Pauline
Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504 Digestion by NotI : Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI We use 18uL of this solution with 2uL plasmid solution Digestion is process during 1h30 at 37°C
Electrophoresis
by Johan, Audrey, Pauline
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
New culture
by Pauline, Audrey
New liquid cultures of:
- K1493504
2x 5ml LB + 10μl Chloramphenicol + 1 bacteria colonies. We incubate cultures at 37°C.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seon-Koo, Audrey, Coralie, Pauline