Difference between revisions of "Team:BostonU/App 2/Results"
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<p>We were so excited to have researched SaCas9 and found potentially viable split sites. We were able to create 16 different split sites within the sequence and cloned most of these constructs into the FKBP/FRB dimerizable domains. Utilizing transient transfection we tested the full SaCas9 and traffic light reporter however there was neither GFP nor mCherry expressed, so we concluded that no double strand break had been made. We hypothesize that there was likely an issue with our choice of target sequence within the traffic light reporter. Below we have results from our flow cytometry run showing arbitrary units of fluorescence.<p> | <p>We were so excited to have researched SaCas9 and found potentially viable split sites. We were able to create 16 different split sites within the sequence and cloned most of these constructs into the FKBP/FRB dimerizable domains. Utilizing transient transfection we tested the full SaCas9 and traffic light reporter however there was neither GFP nor mCherry expressed, so we concluded that no double strand break had been made. We hypothesize that there was likely an issue with our choice of target sequence within the traffic light reporter. Below we have results from our flow cytometry run showing arbitrary units of fluorescence.<p> | ||
− | <center><img style="height: | + | <center><img style="height:50%; width:50%;" src="https://static.igem.org/mediawiki/2015/thumb/a/aa/Sacas9_gfp_finalized.png/800px-Sacas9_gfp_finalized.png" /> |
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− | <img style= "height: | + | <img style= "height:50%; width:50%;" src="https://static.igem.org/mediawiki/2015/thumb/2/2d/Sacas9_mcherry_finalized.png/800px-Sacas9_mcherry_finalized.png" /> |
<center> | <center> | ||
Revision as of 22:37, 18 September 2015
Motivation | Design | Results |