Difference between revisions of "Team:Korea U Seoul/Requirement/Medal criteria/content"

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             <h2>1.Register for iGEM, have a great summer, and attend the Giant Jamboree.</h2>
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    Sortases, enzymes that recognize and cleave the specific sorting signal of secreted proteins to form isopeptide bonds between the secreted proteins and polypeptides, function as protein ligase to form the cell-wall surface of gram-positive bacteria. In case of <i>C.diphtheriae</i>, which belongs to the same genus of our experimental bacteria <i>Corynebacterium glutamicum</i>, has total of 6 sortases, 5 pilus specific sortases (Srt A,B,C,D,E) and 1 housekeeping sortase (Srt F) involved in the formation of all types of pili. <br />
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              <p>We have registered for iGEM and developed our project since January, 2015. Especially, during the summer vacation, our team members learned the knowledge about python, advanced biology and how to create web app with visualization function. Also, we learned how to work in a team with many interactions. You can see the details in our notebook page in wiki. (Notebook page link) We will present our project in Giant Jamboree.</p>
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There are 9 types of pillins that comprise pilus; Spa A, B, C, D, E, F, G, H, I, J. SrtA build up SpaA-type pili, which is composed of Spa A, B and C. Srt B/C and Srt d/E each forms SpaD-type pili and SpaH-type pili, which is made up of Spa D, E, F and Spa H, I, J respectively. Like this, the names of each pili types are generally from the names of many Spa proteins. <br />
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            <h2>2.Complete the Judging form.</h2>
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The picture below shows the process of SpaA type pili formation, using SrtA. Sortase class A enzymes recognize the sequence LPXTG at the carboxyl terminus of surface protein precursors. Cystein of SrtA recognizes LPXTG motif of SpaC, cleaves between T and G, forming SpaC-SrtA intermediate via nucleophilic attack. This intermediate is again attacked by lysine of SrtA bounded SpaA, and the process is continued to form SpaC–SpaAn–SrtA intermediates.<br />
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Nucleophilic attack by Lysine of SrtF bounded SpaB form SpaC-SpaA(n)-SpaB-SrtF intermediate. The product of this SrtA reaction is covalently linked to lipidⅡ and is then incorporated into the cell wall envelope, terminating the formation of SpaA-type pilus.
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We have completed the Judging Form.
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(Architects at the bacterial surface — sortases and the assembly of pili with isopeptide bonds <i>Antoni P. A. Hendrickx, Jonathan M. Budzik, So-Young Oh and Olaf Schneewind</i>)
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            <h2>3.Create and share a Description of the team's project using the iGEM wiki, and document the team's parts (if any) using the Registry of Standard Biological Parts.</h2>
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              <p>We have described our team’s project on iGEM wiki and documented our team’s parts.</p>
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            <h2>4.Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for more information.<h2>
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              <p>We have prepared a fascinating poster for the iGEM Jamboree. If you have interest in our project, come and listen at the Giant Jamboree.</p>
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            <h2>5.Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.<h2>
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              <p>We have created attribution page that describes clear attribution of each aspect of our project. For more details, please visit attribution page.</p>
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              <h2>6. Develop and make available, via the iGEM GitHub page, an open source software that supports Synthetic Biology based on Standard Parts or interacts with the Registry. (For questions about the iGEM Github page, contact software [at] igem [dot] org.)</h2>
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              <p>You can access our software which is open source through GitHub. Our software supports Synthetic Biology with the biobrick of the enzyme gene sequences. More details, please visit our GitHub page.</p>
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Revision as of 22:41, 18 September 2015

Bronze

1.Register for iGEM, have a great summer, and attend the Giant Jamboree.

We have registered for iGEM and developed our project since January, 2015. Especially, during the summer vacation, our team members learned the knowledge about python, advanced biology and how to create web app with visualization function. Also, we learned how to work in a team with many interactions. You can see the details in our notebook page in wiki. (Notebook page link) We will present our project in Giant Jamboree.

2.Complete the Judging form.

We have completed the Judging Form.

3.Create and share a Description of the team's project using the iGEM wiki, and document the team's parts (if any) using the Registry of Standard Biological Parts.

We have described our team’s project on iGEM wiki and documented our team’s parts.

4.Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for more information.

We have prepared a fascinating poster for the iGEM Jamboree. If you have interest in our project, come and listen at the Giant Jamboree.

5.Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.

We have created attribution page that describes clear attribution of each aspect of our project. For more details, please visit attribution page.

6. Develop and make available, via the iGEM GitHub page, an open source software that supports Synthetic Biology based on Standard Parts or interacts with the Registry. (For questions about the iGEM Github page, contact software [at] igem [dot] org.)

You can access our software which is open source through GitHub. Our software supports Synthetic Biology with the biobrick of the enzyme gene sequences. More details, please visit our GitHub page.

Description

The main objective of our project is to construct a novel “protein whip” platform, with which we can make Corynebacterium glutamicum to express other corynebacterium’s pili structure comprised of chains of a protein of our choice. As our first try, we decided to make pili made out of green fluorescence proteins (GFP); in order to do so, we substituted SpaA protein, one of the surface proteins in the Pilin A gene cluster, into green fluorescence protein, and transformed a vector containing the modified Pilin A gene cluster into a C. glutamicum strain.
Our “protein whip” platform is expected to have many practical applications. For example, pili made out of an enzyme, enzyme whip will enable the reaction to take place with high efficiency, for a great number of the enzyme included in the pili will be able to “attack” the reactants simultaneously. Biofilms made of strains of bacteria that express pili comprised of chains of specific amino acids such as histidine or cysteine that readily bind to heavy metals may be utilized to purify water contaminated with heavy metals.
Having a number of potential applications is not the sole merit of our project; by using C. glutamicum instead of widely exploited Escherichia coli, our project also contributes to expanding model organisms used in synthetic biology beyond E. coli.