Difference between revisions of "Team:USTC"

Revision as of 09:15, 8 July 2015

Project Description

In China and other developing countries, abusing antibiotics causes severe antibiotic substances contamination and antibiotic resistance issues. According to latest professional evaluation from Chinese Academy of Science(CAS), China used 162,000 tons of antibiotic substances for human(48%) and animal(52%) in 2013, which is tenfold usage of USA and antibiotic substances in high concentration can be detected from many river basins that may potentially harmful to public health and environment.

Therefore, this year, we USTC developed a novel, original and portable device, which is able to determine trace antibiotics concentration in natural water bodies. We called it CACCI(characterization of trace antibiotics concentration based on chemotaxis and interference).

We firstly construct an antibiotics sensing circuit with antibiotic sensible promoters, quorum sensing molecules and logic amplification parts to test the efficiency of our design. Genetic modification on bacteria including overexpression of exogenous porin or viroporin and inhibition of efflux system using CRISPR will be implemented to condense antibiotics in bacterial bodies.

Then we will establish our CACCI based on genetically engineered chemotaxis circuit and amplification part. Bacteria will be designed with a special transmembrane protein with rare amino acid so that bacteria can be put on a polymer membrane that is able to covalently bind to bacteria with alteration. Thus, slight membrane elastic deformation will be accurately detected by interference and we will use Newton Ring to read out the concentration of antibiotics in sample. Finally CACCI will be developed with a traditional dial and a friendly GUI available for personnel without much professional knowledge and complicated training.

With our CACCI, we hope, antibiotic contamination issue would be treated in specific methods.

Hey, contact us:

Welcome to iGEM 2015!

Your team has been approved and you are ready to start the iGEM season!

Before you start:

Please read the following pages:

• Requirements page
• Wiki Requirements page

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Click here to edit this page!

See tips on how to edit your wiki on the Template Documentation page.

Templates

This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the Template Documentation page.

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

• State your accomplishments! Tell people what you have achieved from the start.
• Be clear about what you are doing and how you plan to do this.
• You have a global audience! Consider the different backgrounds that your users come from.
• Make sure information is easy to find; nothing should be more than 3 clicks away.
• Avoid using very small fonts and low contrast colors; information should be easy to read.
• Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2015 calendar
• Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

• 2014 SDU Denmark
• 2014 Aalto-Helsinki
• 2014 LMU-Munich
• 2014 Michigan
• 2014 ITESM-Guadalajara
• 2014 SCU-China

Uploading pictures and files

You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)