Difference between revisions of "Team:York/Part Collection"

 
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<div class="col-md-1"></div>
 
<div class="col-md-1"></div>
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<div class="col-md-7">
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<div class="col-md-10">
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<table >
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<table width="100%">
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  <col style="width:30%">
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  <col style="width:10%">
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  <col style="width:10%">
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  <col style="width:35%">
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  <col style="width:5%">
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  <col style="width:5%">
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  <col style="width:5%">
 
   <tr>
 
   <tr>
−
     <td>Source Organism</td>
+
     <td><b>Source Organism</b></td>
−
     <td>Gene</td>
+
     <td><b>Gene</b></td>
−
     <td>Part Name</td>
+
     <td><b>Part Name</b></td>
−
     <td>Function</td>
+
     <td class="width"><b>Function</b></td>
−
     <td>Characterised?</td>
+
     <td><b>Characterised</b></td>
−
     <td>Sequenced?</td>
+
     <td><b>Sequenced</b></td>
−
     <td>Submitted?</td>
+
     <td><b>Submitted</b></td>
 
   </tr>
 
   </tr>
−
  <tr>
+
<tr>
−
     <td><i>Escherichia coli</i></td>
+
     <td><b>Expression vector “pAdapt” in pSB1C3</b></td>
−
     <td>EcPPK</td>
+
     <td>lacZalpha</td>
−
     <td>BBa_K1807002</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807000">BBa_K1807000</a></td>
−
     <td>Phosphate Kinase</td>
+
     <td class="width">Used as cloning vector</td>
−
     <td></td>
+
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
   </tr>
 
   </tr>
 
<tr>
 
<tr>
−
     <td><i>Escherichia coli</i></td>
+
     <td><i><b>Escherichia coli</b></i></td>
 
     <td>EcPPX</td>
 
     <td>EcPPX</td>
−
     <td>BBa_K1807001</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807001">BBa_K1807001</a></td>
−
     <td>Phosphate Kinase</td>
+
    <td class="width">Phosphate Kinase</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
    <td>✔</td>
 +
  </tr>
 +
  <tr>
 +
    <td><i><b>Escherichia coli</b></i></td>
 +
    <td>EcPPK</td>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807002">BBa_K1807002</a></td>
 +
     <td class="width">Phosphate Kinase</td>
 
     <td></td>
 
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Escherichia coli</i></td>
+
     <td><i><b>Escherichia coli</b></i></td>
 
     <td>EcPstSCAB</td>
 
     <td>EcPstSCAB</td>
 
     <td></td>
 
     <td></td>
−
     <td>Phosphate specific transporter</td>
+
     <td class="width">Phosphate specific transporter</td>
 
     <td></td>
 
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
−
     <td>✔</td>
+
     <td></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Kingella oralis</i></td>
+
     <td><i><b>Kingella oralis</b></i></td>
 
     <td>KoPPK</td>
 
     <td>KoPPK</td>
−
     <td>BBa_K1807006</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807006">BBa_K1807006</a></td>
−
     <td>PolyPhosphate Kinase</td>
+
     <td class="width">PolyPhosphate Kinase</td>
 
     <td></td>
 
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Sinorhizobium meliloti</i></td>
+
     <td><i><b>Sinorhizobium meliloti</b></i></td>
 
     <td>SmPstSCAB</td>
 
     <td>SmPstSCAB</td>
 
     <td></td>
 
     <td></td>
−
     <td>Phosphate specific transporter</td>
+
     <td class="width">Phosphate specific transporter</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Candidatus accumulibacter phosphatis</i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
−
    <td>ApPstSCAB</td>
+
−
    <td>BBa_K1807007</td>
+
−
    <td>Phosphate specific transporter</td>
+
−
    <td></td>
+
−
    <td>✔</td>
+
−
    <td>✔</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td><i>Candidatus accumulibacter phosphatis</i></td>
+
 
     <td>ApPPK BA-91</td>
 
     <td>ApPPK BA-91</td>
−
     <td>BBa_K1807003</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807003">BBa_K1807003</a></td>
−
     <td>PolyPhosphate kinase</td>
+
     <td class="width">PolyPhosphate kinase</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Candidatus accumulibacter phosphatis</i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
 
     <td>ApPPK SK-12</td>
 
     <td>ApPPK SK-12</td>
−
     <td>BBa_K1807004</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807004">BBa_K1807004</a></td>
−
     <td>PolyPhosphate kinase</td>
+
     <td class="width">PolyPhosphate kinase</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><i>Candidatus accumulibacter phosphatis</i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
 
     <td>ApPPK UW-1</td>
 
     <td>ApPPK UW-1</td>
−
     <td>BBa_K1807005</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807005">BBa_K1807005</a></td>
−
     <td>PolyPhosphate kinase</td>
+
     <td class="width">PolyPhosphate kinase</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
   </tr>
 
   </tr>
−
<tr>
+
  <tr>
−
     <td>Expression vector “pAdapt” in pSB1C3</td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
−
     <td>lacZalpha</td>
+
     <td>ApPstSCAB</td>
−
     <td>BBa_K1807000</td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807007">BBa_K1807007</a></td>
−
     <td>Used as cloning vector</td>
+
     <td class="width">Phosphate specific transporter</td>
−
     <td>✔</td>
+
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
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<p>Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of functionality of the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807000">BBa_K1807000</a> construct.</p>
 +
<img src="https://static.igem.org/mediawiki/2015/d/d5/BBa_K1807000.jpg" height="50%" width="50%" class="border">
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Latest revision as of 23:19, 18 September 2015

Part Collection

Source Organism Gene Part Name Function Characterised Sequenced Submitted
Expression vector “pAdapt” in pSB1C3 lacZalpha BBa_K1807000 Used as cloning vector ✔ ✔ ✔
Escherichia coli EcPPX BBa_K1807001 Phosphate Kinase ✔ ✔
Escherichia coli EcPPK BBa_K1807002 Phosphate Kinase ✔ ✔
Escherichia coli EcPstSCAB Phosphate specific transporter ✔
Kingella oralis KoPPK BBa_K1807006 PolyPhosphate Kinase ✔ ✔
Sinorhizobium meliloti SmPstSCAB Phosphate specific transporter ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK BA-91 BBa_K1807003 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK SK-12 BBa_K1807004 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPPK UW-1 BBa_K1807005 PolyPhosphate kinase ✔ ✔ ✔
Candidatus Accumulibacter phosphatis ApPstSCAB BBa_K1807007 Phosphate specific transporter ✔ ✔

Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of functionality of the BBa_K1807000 construct.