Difference between revisions of "Team:York/Part Collection"

 
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Line 50: Line 50:
 
     <td><b>Submitted</b></td>
 
     <td><b>Submitted</b></td>
 
   </tr>
 
   </tr>
  <tr>
+
<tr>
     <td><i><b>Escherichia coli</b></i></td>
+
     <td><b>Expression vector “pAdapt” in pSB1C3</b></td>
     <td>EcPPK</td>
+
     <td>lacZalpha</td>
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807002">BBa_K1807002</a></td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807000">BBa_K1807000</a></td>
     <td class="width">Phosphate Kinase</td>
+
     <td class="width">Used as cloning vector</td>
     <td></td>
+
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
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     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807001">BBa_K1807001</a></td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807001">BBa_K1807001</a></td>
 
     <td class="width">Phosphate Kinase</td>
 
     <td class="width">Phosphate Kinase</td>
 +
    <td></td>
 +
    <td>✔</td>
 +
    <td>✔</td>
 +
  </tr>
 +
  <tr>
 +
    <td><i><b>Escherichia coli</b></i></td>
 +
    <td>EcPPK</td>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807002">BBa_K1807002</a></td>
 +
    <td  class="width">Phosphate Kinase</td>
 
     <td></td>
 
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td><i><b>Candidatus accumulibacter phosphatis</b></i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
    <td>ApPstSCAB</td>
+
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807007">BBa_K1807007</a></td>
+
    <td class="width">Phosphate specific transporter</td>
+
    <td></td>
+
    <td>✔</td>
+
    <td>✔</td>
+
  </tr>
+
  <tr>
+
    <td><i><b>Candidatus accumulibacter phosphatis</b></i></td>
+
 
     <td>ApPPK BA-91</td>
 
     <td>ApPPK BA-91</td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807003">BBa_K1807003</a></td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807003">BBa_K1807003</a></td>
Line 114: Line 114:
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td><i><b>Candidatus accumulibacter phosphatis</b></i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
 
     <td>ApPPK SK-12</td>
 
     <td>ApPPK SK-12</td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807004">BBa_K1807004</a></td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807004">BBa_K1807004</a></td>
Line 123: Line 123:
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td><i><b>Candidatus accumulibacter phosphatis</b></i></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
 
     <td>ApPPK UW-1</td>
 
     <td>ApPPK UW-1</td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807005">BBa_K1807005</a></td>
 
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807005">BBa_K1807005</a></td>
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     <td>✔</td>
 
     <td>✔</td>
 
   </tr>
 
   </tr>
<tr>
+
  <tr>
     <td><b>Expression vector “pAdapt” in pSB1C3</b></td>
+
     <td><i><b>Candidatus Accumulibacter phosphatis</b></i></td>
     <td>lacZalpha</td>
+
     <td>ApPstSCAB</td>
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807000">BBa_K1807000</a></td>
+
     <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807007">BBa_K1807007</a></td>
     <td class="width">Used as cloning vector</td>
+
     <td class="width">Phosphate specific transporter</td>
     <td></td>
+
     <td></td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
 
     <td>✔</td>
Line 148: Line 148:
  
  
<p>Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of transformation with our SmPst genes.</p>
+
<p>Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of functionality of the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1807000">BBa_K1807000</a> construct.</p>
 
<img src="https://static.igem.org/mediawiki/2015/d/d5/BBa_K1807000.jpg" height="50%" width="50%" class="border">
 
<img src="https://static.igem.org/mediawiki/2015/d/d5/BBa_K1807000.jpg" height="50%" width="50%" class="border">
  

Latest revision as of 23:19, 18 September 2015

Part Collection

Source Organism Gene Part Name Function Characterised Sequenced Submitted
Expression vector “pAdapt” in pSB1C3 lacZalpha BBa_K1807000 Used as cloning vector
Escherichia coli EcPPX BBa_K1807001 Phosphate Kinase
Escherichia coli EcPPK BBa_K1807002 Phosphate Kinase
Escherichia coli EcPstSCAB Phosphate specific transporter
Kingella oralis KoPPK BBa_K1807006 PolyPhosphate Kinase
Sinorhizobium meliloti SmPstSCAB Phosphate specific transporter
Candidatus Accumulibacter phosphatis ApPPK BA-91 BBa_K1807003 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPPK SK-12 BBa_K1807004 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPPK UW-1 BBa_K1807005 PolyPhosphate kinase
Candidatus Accumulibacter phosphatis ApPstSCAB BBa_K1807007 Phosphate specific transporter

Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of functionality of the BBa_K1807000 construct.