Difference between revisions of "Team:KU Leuven/Research/Results"
Laetitia VW (Talk | contribs) |
Laetitia VW (Talk | contribs) |
||
Line 333: | Line 333: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th > | + | <th></th> |
− | <th> | + | <th>0.10</th> |
+ | <th>0.09</th> | ||
+ | <th>0.08</th> | ||
+ | <th>0.07</th> | ||
+ | <th>0.06</th> | ||
+ | <th>0.05</th> | ||
+ | <th>0.04</th> | ||
+ | <th>0.03</th> | ||
+ | <th>0.02</th> | ||
+ | <th>0.01</th> | ||
+ | <th>0.00</th> | ||
</tr> | </tr> | ||
<tr class="lightrow"> | <tr class="lightrow"> | ||
− | <td> | + | <th>T1 </br> (corrected)</th> |
− | <td> | + | <td>0.126</td> |
− | <td> | + | <td>0.072</td> |
+ | <td>0.110</td> | ||
+ | <td>0.093</td> | ||
+ | <td>0.057</td> | ||
+ | <td>0.083</td> | ||
+ | <td>0.052</td> | ||
+ | <td>0.049</td> | ||
+ | <td>0.076</td> | ||
+ | <td>0.011</td> | ||
+ | <td>0.000</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>< | + | <th>T2 </br> (corrected)</th> |
− | <td> | + | <td>0.130</td> |
− | <td> | + | <td>0.073</td> |
+ | <td>0.110</td> | ||
+ | <td>0.076</td> | ||
+ | <td>0.057</td> | ||
+ | <td>0.085</td> | ||
+ | <td>0.048</td> | ||
+ | <td>0.043</td> | ||
+ | <td>0.076</td> | ||
+ | <td>0.008</td> | ||
+ | <td>0.000</td> | ||
</tr> | </tr> | ||
<tr class="lightrow"> | <tr class="lightrow"> | ||
− | <td> | + | <th>T3 </br> (corrected)</th> |
− | <td>0</td> | + | <td>0.121</td> |
− | <td> | + | <td>0.071</td> |
+ | <td>0.078</td> | ||
+ | <td>0.091</td> | ||
+ | <td>0.054</td> | ||
+ | <td>0.083</td> | ||
+ | <td>0.053</td> | ||
+ | <td>0.049</td> | ||
+ | <td0.051></td> | ||
+ | <td>0.008</td> | ||
+ | <td>0.000</td> | ||
</tr> | </tr> | ||
<tr> | <tr> |
Revision as of 23:22, 18 September 2015
![](https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg)
Results
Leucine detection
The standard curve from 0 to 100 µM did not give a linear relationship. Our working method needs optimisation. Because the enzymes are from other organisms than mentioned in Kugimiya and Fukada (2015), it is possible that the enzymes have another efficiency and as a consequence need to have another ratio (substrates over enzyme). Additionally, we did not have the same equipment as described in the article: we had to manually pipet the luminol solution. This possibly means that the measurements have a delay.
Due to a lack of time, we couldn’t complete the plasmid assembly and therefore, we were not able to proceed the quantification of leucine.
In comparison to HPLC, the chosen method would be less time consuming without the need of specialized equipment.
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be