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− | <img class="grpelem" id="u6162-4" src="https://static.igem.org/mediawiki/2014hs/1/1e/U6162-4.png" alt="Odor- Let it Die" width="175" height="42"/><!-- rasterized frame -->
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− | <p> </p>
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− | <ul class="MenuBar clearfix grpelem" id="menuu468"><!-- horizontal box -->
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u617" href="index.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u619-4"><!-- content --><p>Home</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u624" href="team.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u625-4"><!-- content --><p>Team</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u631" href="project.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u632-4"><!-- content --><p>Project</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu MuseMenuActive clearfix colelem" id="u638" href="bio-brick.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u639-4"><!-- content --><p>BioBrick</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u652" href="modeling.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u653-4"><!-- content --><p>Modeling</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u5721" href="safety.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u5722-4"><!-- content --><p>Safety</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u645" href="human-practices.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u646-4"><!-- content --><p>Human Practices</p></div></a>
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− | <a class="nonblock nontext MenuItem MenuItemWithSubMenu clearfix colelem" id="u674" href="media.html"><!-- horizontal box --><div class="MenuItemLabel NoWrap clearfix grpelem" id="u676-4"><!-- content --><p>Media</p></div></a>
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− | </li>
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− | </ul>
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− | <div class="browser_width grpelem" id="u1681"><!-- simple frame --></div>
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− | </div>
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− | <div class="mse_pre_init" id="u2764"><!-- simple frame --></div>
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− | <div class="clearfix colelem" id="pu3615"><!-- group -->
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− | <img class="grpelem" id="u2740-4" src="https://static.igem.org/mediawiki/2014hs/0/0f/U2740-4.png" alt="Bio-Bricks" width="396" height="60"/><!-- rasterized frame -->
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− | </div>
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− | <div class="shadow Ribbon-Large clearfix browser_width colelem" id="u3442-4"><!-- content -->
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− | <h1 id="u3442-2">Biobrick Map- Click link to go to biobrick description</h1>
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− | </div>
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− | <div class="clearfix colelem" id="u6226-16"><!-- content -->
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− | <p>Part Registr<span id="u6226-2">y</span> Name<span id="u6226-4"> </span>Attribute<span id="u6226-6"> </span>Part (E-X-Part-S-P) <span id="u6226-8"> </span>Part Length<span id="u6226-10"> </span>Gene<span id="u6226-12"> </span> Gene Length</p>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3575" href="bio-brick.html#bb02"><!-- image --><img class="block" id="u3575_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3578" href="bio-brick.html#bb03"><!-- image --><img class="block" id="u3578_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3581" href="bio-brick.html#bb004"><!-- image --><img class="block" id="u3581_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3584" href="bio-brick.html#bb05"><!-- image --><img class="block" id="u3584_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3587" href="bio-brick.html#bb06"><!-- image --><img class="block" id="u3587_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3590" href="bio-brick.html#bb07"><!-- image --><img class="block" id="u3590_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3593" href="bio-brick.html#bb08"><!-- image --><img class="block" id="u3593_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3611" href="bio-brick.html#bb09"><!-- image --><img class="block" id="u3611_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3596" href="bio-brick.html#bb10"><!-- image --><img class="block" id="u3596_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3599" href="bio-brick.html#bb11"><!-- image --><img class="block" id="u3599_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3602" href="bio-brick.html#bb12"><!-- image --><img class="block" id="u3602_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3605" href="bio-brick.html#bb13"><!-- image --><img class="block" id="u3605_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | <a class="nonblock nontext anim_swing pinned-colelem" id="u3608" href="bio-brick.html#bb14"><!-- image --><img class="block" id="u3608_img" src="https://static.igem.org/mediawiki/2014hs/6/6e/1402683735_40_hyperlink.png" alt="" width="18" height="18"/></a>
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− | </div>
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− | <div class="clip_frame grpelem" id="u2915"><!-- image -->
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− | <img class="block" id="u2915_img" src="https://static.igem.org/mediawiki/2014hs/4/42/Chart.jpg" alt="" width="1015" height="382"/>
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− | </div>
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− | </div>
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− | <div class="clearfix colelem" id="pu3197-4"><!-- group -->
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− | <div class="shadow Ribbon-Large clearfix browser_width grpelem" id="u3197-4"><!-- content -->
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− | <h1 id="u3197-2">Description</h1>
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− | </div>
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− | <a class="anchor_item grpelem" id="biobrickdesign"></a>
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− | </div>
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− | <div class="clearfix colelem" id="pu3380"><!-- group -->
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− | <div class="clearfix grpelem" id="u3380"><!-- column -->
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− | <div class="clearfix colelem" id="u3315-4"><!-- content -->
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− | <p>Biobrick Design</p>
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− | </div>
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− | <div class="colelem" id="u3316"><!-- simple frame --></div>
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− | </div>
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− | <a class="anchor_item grpelem" id="bb01"></a>
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− | </div>
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− | <div class="clearfix colelem" id="u3466"><!-- column -->
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− | <div class="clearfix colelem" id="pu3317-21"><!-- group -->
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− | <div class="clearfix grpelem" id="u3317-21"><!-- content -->
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− | <p id="u3317-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256001">pSB1C3-Plac-NB (Part: BBa_K1256001)</a></p>
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− | <p> </p>
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− | <p id="u3317-8"><span id="u3317-6">BENEFIT: </span>cloning genes with NheI & BamHI sites, easily exchange RFP gene</p>
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− | <p id="u3317-11"><span id="u3317-9">BIOBRICK LIGATION</span>:</p>
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− | <p id="u3317-13">- “X-P” ligated to “S-P” ( X, S are compatible): standard part</p>
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− | <p id="u3317-15">- “E-N” ligated to “E-X” (N, X are compatible): to exchange front part (Fig 1. A)</p>
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− | <p id="u3317-17">- “N-P” ligated to “S-P” (N, S are compatible): to exchange back part (Fig 1. B)</p>
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− | <p> </p>
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− | <p> </p>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3320"><!-- image -->
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− | <img class="block" id="u3320_img" src="https://static.igem.org/mediawiki/2014hs/7/70/Psb1c3-plac-nb.png" alt="" width="507" height="174"/>
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− | </div>
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− | </div>
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− | <div class="clearfix colelem" id="u3352-9"><!-- content -->
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− | <p>Fig 1. BioBrick “part in part” (image below)</p>
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− | <p> </p>
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− | <p id="u3352-7">(A<span id="u3352-5">)</span>(B)</p>
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− | </div>
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− | <div class="clearfix colelem" id="pu3358"><!-- group -->
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− | <div class="clip_frame grpelem" id="u3358"><!-- image -->
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− | <img class="block" id="u3358_img" src="https://static.igem.org/mediawiki/2014hs/f/f5/Biobrick_fig_b.png" alt="" width="297" height="305"/>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3353"><!-- image -->
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− | <img class="block" id="u3353_img" src="https://static.igem.org/mediawiki/2014hs/0/0d/Biobrick_fig_a.png" alt="" width="297" height="305"/>
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− | </div>
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− | </div>
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− | </div>
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− | <a class="anchor_item colelem" id="bb02"></a>
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− | <div class="clearfix colelem" id="u3493"><!-- group -->
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− | <div class="clearfix grpelem" id="u3366-16"><!-- content -->
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− | <p id="u3366-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256002">pSB1C3-Plac-NB-Cm-MN (BBa_K1256002)</a></p>
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− | <p id="u3366-5"> </p>
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− | <p id="u3366-8"><span id="u3366-6">BENEFIT:</span> cloning genes with MfeI & NsiI sites under CmR cassette</p>
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− | <p id="u3366-10">BIOBRICK LIGATION:</p>
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− | <p id="u3366-12">- “M-Ns” ligated to “E-P” ( M, E are compatible; Ns, P are compatible)</p>
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− | <p> </p>
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− | <p> </p>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3487"><!-- image -->
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− | <img class="block" id="u3487_img" src="https://static.igem.org/mediawiki/2014hs/0/02/Psb1c3-plac-nb-cm-mn.png" alt="" width="519" height="193"/>
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− | </div>
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− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb03"></a>
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− | <div class="clearfix colelem" id="pattack"><!-- group -->
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− | <a class="anchor_item grpelem" id="attack"></a>
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− | <div class="clearfix grpelem" id="u3512"><!-- group -->
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− | <div class="clearfix grpelem" id="u3374-20"><!-- content -->
| + | |
− | <p id="u3374-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256003">pSB1C3-Plac-SS-NB (Part: BBa_K1256003)</a></p>
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− | <p id="u3374-5"> </p>
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− | <p id="u3374-8"><span id="u3374-6">Secretion signal (SS):</span> OmpA SS from E. coli</p>
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− | <p id="u3374-11"><span id="u3374-9">BENEFIT:</span> cloning genes with NheI & BamHI sites, easily exchange RFP gene</p>
| + | |
− | <p id="u3374-13">BIOBRICK LIGATION:</p>
| + | |
− | <p id="u3374-15">- “X-P” ligated to “S-P” or “N-P” ligated to “E-X” (X, S, N are compatible)</p>
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− | <p> </p>
| + | |
− | <p> </p>
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− | <p> </p>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3507"><!-- image -->
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− | <img class="block" id="u3507_img" src="https://static.igem.org/mediawiki/2014hs/0/05/Psb1c3-plac-ss-nb.png" alt="" width="504" height="173"/>
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− | </div>
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− | </div>
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− | </div>
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− | <div class="clearfix colelem" id="pbb004"><!-- group -->
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− | <a class="anchor_item grpelem" id="bb004"></a>
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− | <div class="clearfix grpelem" id="u3381"><!-- column -->
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− | <div class="clearfix colelem" id="u3383-4"><!-- content -->
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− | <p>Attack</p>
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− | </div>
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− | <div class="colelem" id="u3382"><!-- simple frame --></div>
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− | </div>
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− | </div>
| + | |
− | <div class="clearfix colelem" id="u3505"><!-- column -->
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− | <div class="clearfix colelem" id="u3385-9"><!-- content -->
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− | <p id="u3385-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256004">pSB1C3-Plac-SS-Cecropin-MprF (BBa_K1256004)</a></p>
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− | <p id="u3385-5"> </p>
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− | <p id="u3385-7">MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the codon optimization tool provided by Integrated DNA Technologies (IDT).</p>
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− | </div>
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− | <div class="clip_frame colelem" id="u3494"><!-- image -->
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− | <img class="block" id="u3494_img" src="https://static.igem.org/mediawiki/2014hs/e/ec/Psb1c3-plac-ss-cecropin-mprf.png" alt="" width="510" height="174"/>
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− | </div>
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− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb05"></a>
| + | |
− | <div class="clearfix colelem" id="u3551"><!-- group -->
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− | <div class="clearfix grpelem" id="u3398-11"><!-- content -->
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− | <p id="u3398-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256005">pSB1C3-Plac-SS-Magainin-MprF (BBa_K1256005)</a></p>
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− | <p id="u3398-5"> </p>
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− | <p id="u3398-7">MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of magainin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of magainin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the codon optimization tool provided by Integrated DNA Technologies (IDT).</p>
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− | <p> </p>
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− | <p> </p>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3499"><!-- image -->
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− | <img class="block" id="u3499_img" src="https://static.igem.org/mediawiki/2014hs/b/b0/Psb1c3-plac-ss-magainin-mprf.png" alt="" width="510" height="173"/>
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− | </div>
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− | </div>
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− | <div class="clearfix colelem" id="pdecomposing"><!-- group -->
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− | <a class="anchor_item grpelem" id="decomposing"></a>
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− | <div class="clearfix grpelem" id="u3550"><!-- group -->
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− | <div class="clearfix grpelem" id="u3410-5"><!-- content -->
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− | <p>Colony PCR was performed to check the plasmid (Right Photo). The cecropin and magainin sequences were further confirmed by sequencing.</p>
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− | <p> </p>
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− | </div>
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− | <div class="clip_frame grpelem" id="u3405"><!-- image -->
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− | <img class="block" id="u3405_img" src="https://static.igem.org/mediawiki/2014hs/c/c1/Gel_attack.png" alt="" width="360" height="159"/>
| + | |
− | </div>
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− | </div>
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− | </div>
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− | <div class="clearfix colelem" id="pbb06"><!-- group -->
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− | <a class="anchor_item grpelem" id="bb06"></a>
| + | |
− | <div class="clearfix grpelem" id="u3401"><!-- column -->
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− | <div class="clearfix colelem" id="u3402-4"><!-- content -->
| + | |
− | <p>Decomposing</p>
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− | </div>
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− | <div class="colelem" id="u3403"><!-- simple frame --></div>
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− | </div>
| + | |
− | </div>
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− | <div class="clearfix colelem" id="u3543"><!-- column -->
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− | <div class="clearfix colelem" id="u3411-7"><!-- content -->
| + | |
− | <p id="u3411-2"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256006">pSB1C3-Plac-SS-Protease (BBa_K1256006)</a></p>
| + | |
− | <p id="u3411-3"> </p>
| + | |
− | <p id="u3411-5">The protease coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to three more sites on the gene coding region.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3533"><!-- image -->
| + | |
− | <img class="block" id="u3533_img" src="https://static.igem.org/mediawiki/2014hs/b/b5/Psb1c3-plac-ss-protease.png" alt="" width="510" height="176"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb07"></a>
| + | |
− | <div class="clearfix colelem" id="u3544"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3412-9"><!-- content -->
| + | |
− | <p id="u3412-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256007">pSB1C3-Plac-SS-Lipase (BBa_K1256007)</a></p>
| + | |
− | <p id="u3412-5"> </p>
| + | |
− | <p id="u3412-7">The lipase coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and NsiI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) with NheI and PstI, leaving a scar of NsiI/PsiI in the end.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3528"><!-- image -->
| + | |
− | <img class="block" id="u3528_img" src="https://static.igem.org/mediawiki/2014hs/d/da/Psb1c3-plac-ss-lipase.png" alt="" width="510" height="179"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb08"></a>
| + | |
− | <div class="clearfix colelem" id="u3545"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3414-9"><!-- content -->
| + | |
− | <p id="u3414-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256008">pSB1C3-Plac-SS-DNase (BBa_K1256008)</a></p>
| + | |
− | <p id="u3414-5"> </p>
| + | |
− | <p id="u3414-7">The DNase coding sequence (cds) of Stenotrophomonas maltophilia D457 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to two more sites on the gene coding region.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3518"><!-- image -->
| + | |
− | <img class="block" id="u3518_img" src="https://static.igem.org/mediawiki/2014hs/e/ef/Psb1c3-plac-ss-dnase.png" alt="" width="510" height="176"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb09"></a>
| + | |
− | <div class="clearfix colelem" id="u3546"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3415-9"><!-- content -->
| + | |
− | <p id="u3415-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256009">pSB1C3-Plac-SS-RNase (BBa_K1256009)</a></p>
| + | |
− | <p id="u3415-5"> </p>
| + | |
− | <p id="u3415-7">The RNase coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and BglII and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) cut by NheI and BamHI. PstI site can no longer be used in this plasmid due to one more site on the gene coding region.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3538"><!-- image -->
| + | |
− | <img class="block" id="u3538_img" src="https://static.igem.org/mediawiki/2014hs/3/3f/Psb1c3-plac-ss-rnase.png" alt="" width="510" height="171"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb10"></a>
| + | |
− | <div class="clearfix colelem" id="u3547"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3419-9"><!-- content -->
| + | |
− | <p id="u3419-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256010">pSB1C3-Plac-SS-Chitinase (BBa_K1256010)</a></p>
| + | |
− | <p id="u3419-5"> </p>
| + | |
− | <p id="u3419-7">The chitinase coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and NsiI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) with NheI and PstI, leaving a scar of NsiI/PsiI in the end.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3513"><!-- image -->
| + | |
− | <img class="block" id="u3513_img" src="https://static.igem.org/mediawiki/2014hs/4/4c/Psb1c3-plac-ss-chitinase.png" alt="" width="510" height="175"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb11"></a>
| + | |
− | <div class="clearfix colelem" id="u3548"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3420-9"><!-- content -->
| + | |
− | <p id="u3420-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256011">pSB1C3-Plac-SS-Glucanase (BBa_K1256011)</a></p>
| + | |
− | <p id="u3420-5"> </p>
| + | |
− | <p id="u3420-7">The glucanase coding sequence (cds) of Bacillus subtilis 168 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with other BioBrick parts with NheI/PstI and SpeI/PstI, or be considered as a standard BioBrick parts (E-X-S-P).</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3523"><!-- image -->
| + | |
− | <img class="block" id="u3523_img" src="https://static.igem.org/mediawiki/2014hs/a/a0/Psb1c3-plac-ss-glucanase.png" alt="" width="510" height="174"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="pselfdestruct"><!-- group -->
| + | |
− | <a class="anchor_item grpelem" id="selfdestruct"></a>
| + | |
− | <div class="clearfix grpelem" id="u3549"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3422-12"><!-- content -->
| + | |
− | <p>Genes of decomposing enzymes were cloned either from Stenotrophomonas maltophilia or Bacillus subtilis. Figure 1 showed the genes were amplified by PCR.</p>
| + | |
− | <p> </p>
| + | |
− | <p>Figure 1. PCR to amplify the genes of decomposing enzymes</p>
| + | |
− | <p> </p>
| + | |
− | <p id="u3422-10">(A<span id="u3422-8">)</span>(B)</p>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="pu3423"><!-- group -->
| + | |
− | <div class="clip_frame grpelem" id="u3423"><!-- image -->
| + | |
− | <img class="block" id="u3423_img" src="https://static.igem.org/mediawiki/2014hs/b/b7/Gel_fig_b.png" alt="" width="367" height="249"/>
| + | |
− | </div>
| + | |
− | <div class="clip_frame grpelem" id="u3428"><!-- image -->
| + | |
− | <img class="block" id="u3428_img" src="https://static.igem.org/mediawiki/2014hs/c/c8/Gel_fig_1.png" alt="" width="377" height="274"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="pbb12"><!-- group -->
| + | |
− | <a class="anchor_item grpelem" id="bb12"></a>
| + | |
− | <div class="clearfix grpelem" id="u3433"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3434-4"><!-- content -->
| + | |
− | <p>Self-destruction</p>
| + | |
− | </div>
| + | |
− | <div class="colelem" id="u3435"><!-- simple frame --></div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="u3486"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3468"><!-- group -->
| + | |
− | <div class="clearfix grpelem" id="u3446-11"><!-- content -->
| + | |
− | <p id="u3446-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256012">pSB1C3-Plac-RFP-Cm-LacI (BBa_K1256012)</a></p>
| + | |
− | <p id="u3446-5"> </p>
| + | |
− | <p id="u3446-7">RBS, LacI coding region and terminators (Part:BBa_Q04121) were amplified by PCR and cut by EcoRI and PstI. The PCR-amplified and enzyme-cut product was introduced to the novel designed plasmid backbone of pSB1C3-Plac-NB-Cm-MN ( Part:BBa_K1256002) with MfeI and NsiI. LacI gene is driven by Chloramphenicol promoter. RFP coding region can be exchanged using NheI and BamHI.</p>
| + | |
− | <p id="u3446-8"> </p>
| + | |
− | <p id="u3446-9"> </p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame grpelem" id="u3456"><!-- image -->
| + | |
− | <img class="block" id="u3456_img" src="https://static.igem.org/mediawiki/2014hs/e/ed/Psb1c3-plac-rfp-cm-laci.png" alt="" width="508" height="203"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="pu3451-9"><!-- group -->
| + | |
− | <div class="clearfix grpelem" id="u3451-9"><!-- content -->
| + | |
− | <p>Figure 1 showed the result of colony PCR (A) and the RFG gene expression induced by IPTG (B).</p>
| + | |
− | <p id="u3451-3"> </p>
| + | |
− | <p id="u3451-7">(A<span id="u3451-5">)</span>(B)</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame grpelem" id="u3476"><!-- image -->
| + | |
− | <img class="block" id="u3476_img" src="https://static.igem.org/mediawiki/2014hs/6/67/Colony_pcr.png" alt="" width="220" height="235"/>
| + | |
− | </div>
| + | |
− | <div class="clip_frame grpelem" id="u3481"><!-- image -->
| + | |
− | <img class="block" id="u3481_img" src="https://static.igem.org/mediawiki/2014hs/9/9a/Pellete.png" alt="" width="220" height="218"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb13"></a>
| + | |
− | <div class="clearfix colelem" id="u3469"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3450-9"><!-- content -->
| + | |
− | <p id="u3450-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256013">pSB1C3-Plac-ccdB-Cm-LacI (BBa_K1256013)</a></p>
| + | |
− | <p id="u3450-5"> </p>
| + | |
− | <p id="u3450-7">ccdB coding region was amplified by PCR from BBa_K145151 and exchanged with RFP on the plasmid of BBa_K1256012.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3461"><!-- image -->
| + | |
− | <img class="block" id="u3461_img" src="https://static.igem.org/mediawiki/2014hs/f/f3/Psb1c3-plac-ccdb-cm-laci.png" alt="" width="508" height="202"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <a class="anchor_item colelem" id="bb14"></a>
| + | |
− | <div class="clearfix colelem" id="u3475"><!-- column -->
| + | |
− | <div class="clearfix colelem" id="u3455-9"><!-- content -->
| + | |
− | <p id="u3455-4"><a class="nonblock" href="http://parts.igem.org/Part:BBa_K1256014">pSB1C3-Pred-ccdB-light sensor (BBa_K1256014)</a></p>
| + | |
− | <p id="u3455-5"> </p>
| + | |
− | <p>Gene expression of ccdB (BBa_K145151), a lethal gene, is regulated by the light. The light-regulated system is composed of light sensor (BBa_K1017101) and light-regulated promoter (BBa_K1017301) designed and constructed by NCTU-Formosa in 2013. Light sensor was first amplified by PCR, cut by EcoRI and PstI, and ligated to pSB1C3-Plac-NB-Cm-MN cut by MfeI and NsiI. Then, light-regulated promoter was amplified by PCR, cut by EcoRI and NheI, and ligated to the resulting vector cut by EcoRI and NheI, which maintains the standard BioBrick assembly rule as well as the flexibility of exchanging RFP cds with NheI and BamHI. The ccdB was amplified by PCR using the forward primer with RBS and assembled to the vector with NheI and BamHI.</p>
| + | |
− | </div>
| + | |
− | <div class="clip_frame colelem" id="u3470"><!-- image -->
| + | |
− | <img class="block" id="u3470_img" src="https://static.igem.org/mediawiki/2014hs/d/d1/Psb1c3-pred-ccdb-light_sensor_%28bba_k1256014%29.png" alt="" width="509" height="207"/>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="clearfix colelem" id="pu3440-18"><!-- group -->
| + | |
− | <div class="clearfix" id="u3440-18"><!-- content -->
| + | |
− | <p id="u3440-4"><a class="nonblock nontext anim_swing pinned-colelem" href="bio-brick.html#biobrickdesign">Biobrick Design</a></p>
| + | |
− | <p id="u3440-8"><a class="nonblock nontext anim_swing pinned-colelem" href="bio-brick.html#attack">Attack</a></p>
| + | |
− | <p id="u3440-12"><a class="nonblock nontext anim_swing pinned-colelem" href="bio-brick.html#decomposing">Decomposing</a></p>
| + | |
− | <p id="u3440-16"><a class="nonblock nontext anim_swing pinned-colelem" href="bio-brick.html#selfdestruct">Self-destruction</a></p>
| + | |
− | </div>
| + | |
− | <div class="clearfix" id="u6182"><!-- column -->
| + | |
− | <div class="position_content" id="u6182_position_content">
| + | |
− | <a class="nonblock nontext anim_swing pinned-colelem" id="u6184" href="bio-brick.html#top"><!-- rasterized frame --><img id="u6184_img" src="https://static.igem.org/mediawiki/2014hs/2/27/To_top_icon-u6165.png" alt="" width="71" height="71"/></a>
| + | |
− | <a class="nonblock nontext anim_swing clearfix pinned-colelem" id="u6183-4" href="bio-brick.html#top"><!-- content --><p>Top</p></a>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div class="verticalspacer"></div>
| + | |
− | <div class="clearfix colelem" id="pu2040-3"><!-- group -->
| + | |
− | <div class="clearfix browser_width grpelem" id="u2040-3"><!-- content -->
| + | |
− | <p> </p>
| + | |
− | </div>
| + | |
− | <a class="nonblock nontext clip_frame grpelem" id="u1692" href="http://www.bertec.com.tw/"><!-- image --><img class="block" id="u1692_img" src="https://static.igem.org/mediawiki/2014hs/b/bf/Bertec_logo-u1692.png" alt="" width="94" height="63"/></a>
| + | |
− | <div class="clearfix grpelem" id="u1698-4"><!-- content -->
| + | |
− | <p>Sponsored by:</p>
| + | |
− | </div>
| + | |
− | <div class="clearfix grpelem" id="u1702-6"><!-- content -->
| + | |
− | <p><a class="nonblock" href="team.html#sponsors">and more</a></p>
| + | |
− | </div>
| + | |
− | <div class="browser_width grpelem" id="u3436"><!-- simple frame --></div>
| + | |
− | <div class="clearfix grpelem" id="u6138-4"><!-- content -->
| + | |
− | <p>Website best viewed with a 1920px wide screen with chrome</p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
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