Difference between revisions of "Team:Aix-Marseille"

 
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<link rel="stylesheet" href="https://2015.igem.org/Team:NRP-UEA-Norwich/mobilecss?action=raw&amp;ctype=text/css" type="text/css">
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<style type="text/css">
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.affix {
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/animate.css/3.4.0/animate.min.css">
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<body>
 
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<header class="header" >
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                <nav class="navbar navbar-default " role="navigation" data-spy="affix" data-offset-top="600">
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                  <div class="navbar-inner"> 
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                    <div class="navbar-header">
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                        <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1">
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                            <span class="sr-only">Toggle navigation</span>
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                            <span class="icon-bar"></span>
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                            <span class="icon-bar"></span>
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                            <span class="icon-bar"></span>
  
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<h2> Welcome to iGEM 2015!! </h2>
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                        </button>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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                        <a href="/Team:Aix Marseille" class="navbar-brand hidden-lg hidden-md hidden-sm" ><https://static.igem.org/mediawiki/2015/6/6e/OHSATfM.png" class="img-responsive" width="80" height="80"></a>
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                    </div>
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                            <div class="collapse navbar-collapse  " id="bs-example-navbar-collapse-1">
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                                <ul class="nav navbar-nav  " id="nav">
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                                <li><a href="/Team:Aix-Marseille"  class="logo"><img src="https://static.igem.org/mediawiki/2015/6/6e/OHSATfM.png" class="img-responsive" width="85" height="85"></a></li>
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                                    <li  ><a href="/Team:Aix-Marseille">Home</a></li>
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                                      <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Project</a>
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                                      <ul class="dropdown-menu">
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                                        <li ><a href="/Team:Aix-Marseille/Project/Description">Overview</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Design">Project design</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Parts">Parts</a></li>                                 
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                                      </ul>
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                                      <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Team</a>
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                                      <ul class="dropdown-menu">
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                                        <li ><a href="/Team:Aix-Marseille/Team">Members</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Supervisors">Supervisors</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Collaborations">Collaborations</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Attributions">Attributions</a></li>                                 
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                                      </ul>
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                                      <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Notebook</a>
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                                      <ul class="dropdown-menu">
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                                        <li ><a href="/Team:Aix-Marseille/Notebook">Calendar</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Results">Results</a></li>
 +
                                        <li ><a href="/Team:Aix-Marseille/Protocols">Protocols</a></li>                                 
 +
                                      </ul>
  
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<h4>Before you start: </h4>
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                                    <li  ><a href="/Team:Aix-Marseille/Practices">Practices</a></li> 
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<p> Please read the following pages:</p>
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<ul>
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                                    <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study</a>
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<li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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                                      <ul class="dropdown-menu">
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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                                        <li ><a href="/Team:Aix-Marseille/Notebook2">Calendar</a></li>
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</ul>
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                                        <li ><a href="/Team:Aix-Marseille/Measurement">Results</a></li>
 +
                                        <li ><a href="/Team:Aix-Marseille/Protocols2">Protocols</a></li>
 +
                                        <li ><a href="/Team:Aix-Marseille/Sequencing/data">Sequencing data</a></li>                                  
 +
                                      </ul>
 +
                                    <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Modeling</a>
 +
                                      <ul class="dropdown-menu">
 +
                                        <li ><a href="/Team:Aix-Marseille/Modeling">Modeling</a></li>
 +
                                        <li ><a href="/Team:Aix-Marseille/Software">Software</a></li>                                 
 +
                                      </ul>   
 +
                                    </li>
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                      <li class="dropdown " id="dropdown"><a href="/Team:Aix-Marseille/Achievement" class="dropdown-toggle"  data-toggle="dropdown">Achievement</a></li>
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                                  </li>
  
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<div class="highlightBox">
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                                </ul>  
−
<h4> Styling your wiki </h4>
+
                               
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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                          </div>
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</div>
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                </div>      
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                </nav>
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            </div>  
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        </div>
  
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<h4> Editing your wiki </h4>
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    </header>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Aix-Marseille&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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<h4>Templates </h4>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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    <!-- start hearo section -->
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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    <section class="bg-1" id="home">
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-12 space10">
  
 +
    <img src="https://static.igem.org/mediawiki/2015/0/04/84NjpEo.png" class="img-responsive mautomargin" width="350" height="150">
 +
   
 +
    </div>
 +
    <div class="col-md-12 space5 text-center">
 +
    <h1 class="f800 head-1" style="margin-bottom: .2em;">NO MORE <span style="color:#8E3B8C">GUM </span> ON OUR <span style="color:#8E3B8C">STREETS<br><br></span></h1>
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 +
    </div>
 +
    </div>
 +
    </div>
 +
  
−
<h4>Tips</h4>
+
    </section>
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
    <!-- end hearo section -->
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<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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−
<li>Have lots of fun! </li>
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</ul>  
+
  
 +
    <div class="clearfix"></div>
 +
      <!-- start project section -->
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    <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <h2 class="title wow bounceInUp"><span style="color:#8E3B8C"><span style="font-family:Armalite rifle">OUR IDEA</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style="font-family:Courier New">Nowadays, chewing gum is the second urban pollutant after cigarette butts. To clean chewing gum, specific engines are used but they are heavy and expensive. The time required for its use is long and they need a large quantity of water. Besides economical aspects, chewing gums have an environmental impact. They are dangerous for the wildlife such as birds. Indeed, believing that it is bread, they eat chewing gums and choke. Regarding these alarming facts, we have decided to create a new environmentally- responsible way to sanitize our streets.</div></p></span></span>
  
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<h4>Inspiration</h4>
+
 
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
    </div>
−
<ul>
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    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
                    <div class="success-work project">
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
                        <div class="success-work-desc">
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                            <img src="https://static.igem.org/mediawiki/2015/3/38/PIm24O7.jpg" class="img-responsive" width="400" height="250">
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
                         
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
                            <a title="Lightbox Example"  href=https://2015.igem.org/Team:Aix-Marseille/Project/Description" class="btn outlinebtn  space10">
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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                            Details !
 +
                          </a>
 +
                        </div>
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                    </div>
 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 
 +
    </section>
 +
    <div class="clearfix"></div>
 +
      <!-- start science section -->
 +
<section style="padding:30px 0px 50px;background-color: #fbf1fb;" "background:#fbf1fb;"="" class="bg-2 arrow_box2;">
 +
 
 +
    <div style ="background:#FBF1FB;">
 +
    <div class="container">
 +
    <div class="row">
 +
 
 +
    <div class="col-md-6 left">
 +
    <h2 class="title wow bounceInUp"><span style ="color:#000000"><span style="font-family:Armalite rifle">OUR PROJECT</span></h2></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Our project is to create an <I>E.coli</I> strain that produces enzymes , which can degrade rubber polymers. To achieve our goal, we will use a combination of three enzymes: a laccase, a lipoxygenase or latex clearing protein (LCP) and a cytochrome C. The laccase known as an oxidoreductase will oxidize the cytochrome C which will be previously light excited. Coupled with this later, the LCP will degrade synthetics polymers found in the chewing gum. </p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">We know that the cytochrome C needs to be maturated and to incorporate a heme into its structure. To optimize the function of the cytochrome C, we will express the gene cluster involved in the cytochrome C maturation and a gene to produce a heme.</p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">In order to have the best chance of success, we selected different enzymes: 4 laccases, 2 LCP and 2 cytochromes C and we will try many combinations.</p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Moreover, we would like to fuse laccases and cytochromes C. But, we were stunned by the lack of information about linker designing. Therefore, we will try to create a tool to easily design linker to make fusion proteins. </p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Once several combinations will be properly cloned into an E.coli strain, we will purify proteins. Then, we will perform assays to test their enzymatic activities by oxygen measurement and spectrophotometry.
 +
</p></span></div></span>
 +
 
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1 space30 text-center">
 +
                    <div class="success-work science">
 +
                        <div class="success-work-desc">
 +
                        <img src="https://static.igem.org/mediawiki/2015/2/2b/WJg8VPU.jpg" class="img-responsive" space200>
 +
                         
 +
                          <a title="Lightbox Example"  href=https://2015.igem.org/Team:Aix-Marseille/Design" class="btn outlinebtn  space50">
 +
                            Details!
 +
                          </a>
 +
                        </div>
 +
                        </div>
 +
                    </div>
 +
   
 +
    </div>
 +
    </div>
 +
    </div>
 +
 
 +
    </section>
 +
   
 +
 
 +
    </section>   
 +
      <!-- start social section -->
 +
    <section style="padding:50px 0px;" class="arrow_box" id="ethics">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-12 text-center">
 +
<h1 class="blue">MORE INFORMATION ON FACEBOOK !</h1>
 +
                    <ul class="list-inline space80 icon">
 +
                        <li><a href="https://www.facebook.com/iGEM.AMU?fref=ts"><img src="https://static.igem.org/mediawiki/2015/e/ed/FacebookAMU.png" width="200" height="200" class="img-grey mautomargin"></a></li>
 +
                    </ul>
 +
 
 +
                </div>
 +
    </div>
 +
    </div>
 +
 
 +
    </section>
 +
      <div class="clearfix"></div>
 +
      <!-- start sponsors section -->
 +
<section style="padding:50px 0px;" class="arrow_box-2">
 +
       
 +
        <div class="container">
 +
            <div class="row">
 +
                <div class="col-md-12 text-center">
 +
                    <h2 class="gray">SPONSORS</h2>
 +
<ul class="list-inline space30 icon">
 +
  <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html">
 +
    <img src="https://static.igem.org/mediawiki/2015/8/87/Corninglogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/18/KlP5G8h.jpg"
 +
    class="img-grey mautomargin" width="200" align="center"></a></li>
 +
  <li><a href="http://www.cnrs.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/8/8e/CNRSlogo.jpg"
 +
    width="200" class="img-grey mautomargin" align="center"></a></li>
 +
  <li><a href="https://www.qiagen.com/fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/f/f1/Qialogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"
 +
    class="img-grey mautomargin" width="200" align="center" ></a></li>
 +
  <li><a href="http://polytech.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"
 +
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    <img src="https://static.igem.org/mediawiki/2015/4/41/PUNXaNj.jpg" align="center"
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    class="img-grey mautomargin" width="200"></a></li>
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    <img src="https://static.igem.org/mediawiki/2015/1/13/StarLablogo.jpg" align="center"
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  <li><a href="http://fr.ambafrance-us.org/">
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    <img src="https://static.igem.org/mediawiki/2015/2/2b/M3ieC2R.jpg" align="center"
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    class="img-grey mautomargin" width="200" height="100"></a></li>
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  <li><a href="https://www.neb.com/">
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    <img src="https://static.igem.org/mediawiki/2015/a/a9/Ll3uagr.jpg" align="center"
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  <li><a href="http://sciences.univ-amu.fr/">
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    class="img-grey mautomargin" width="200"></a></li>
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  <li><a href="https://eu.idtdna.com/site">
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    <img src="https://static.igem.org/mediawiki/2015/d/d9/IDTlogo.jpg" align="center"
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    class="img-grey mautomargin" width="200"></a></li>
 
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                        <p class="fsize18 space10">Chew figth project, for the iGEM competition.</p>
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Latest revision as of 23:50, 18 September 2015

Chew fight

NO MORE GUM ON OUR STREETS

OUR IDEA

Nowadays, chewing gum is the second urban pollutant after cigarette butts. To clean chewing gum, specific engines are used but they are heavy and expensive. The time required for its use is long and they need a large quantity of water. Besides economical aspects, chewing gums have an environmental impact. They are dangerous for the wildlife such as birds. Indeed, believing that it is bread, they eat chewing gums and choke. Regarding these alarming facts, we have decided to create a new environmentally- responsible way to sanitize our streets.

Details !

OUR PROJECT

Our project is to create an E.coli strain that produces enzymes , which can degrade rubber polymers. To achieve our goal, we will use a combination of three enzymes: a laccase, a lipoxygenase or latex clearing protein (LCP) and a cytochrome C. The laccase known as an oxidoreductase will oxidize the cytochrome C which will be previously light excited. Coupled with this later, the LCP will degrade synthetics polymers found in the chewing gum. 

We know that the cytochrome C needs to be maturated and to incorporate a heme into its structure. To optimize the function of the cytochrome C, we will express the gene cluster involved in the cytochrome C maturation and a gene to produce a heme.

In order to have the best chance of success, we selected different enzymes: 4 laccases, 2 LCP and 2 cytochromes C and we will try many combinations.

Moreover, we would like to fuse laccases and cytochromes C. But, we were stunned by the lack of information about linker designing. Therefore, we will try to create a tool to easily design linker to make fusion proteins. 

Once several combinations will be properly cloned into an E.coli strain, we will purify proteins. Then, we will perform assays to test their enzymatic activities by oxygen measurement and spectrophotometry.

Details!

MORE INFORMATION ON FACEBOOK !

SPONSORS

Useful Links

Contact Us

  • MAIL

  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition.