Difference between revisions of "Team:Aix-Marseille"

 
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    <h1 class="f800 head-1" style="margin-bottom: .2em;">NO MORE <span style="color:#8E3B8C">GUM </span> ON OUR <span style="color:#8E3B8C">STREETS<br><br></span></h1>
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 +
    </div>
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 +
    </div>
 
 
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<div id="under_construction"/>
 
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<div id="cadre_description">Nowadays, chewing gum is the second urban pollutant after cigarette butts. To clean chewing gum, specific engines are used but they are heavy and expensive. The time required for its used is long and they need a large quantity of water. Besides economical aspects, chewing gums have an environmental impact. They are dangerous for the wildlife such as birds. Indeed, believing that it is bread, they eat chewing gums and choke. Regarding these alarming facts, we have decided to create a new environmentally- responsible way to sanitize our streets.
 
−
Our project is to create an E.coli strain that produces enzymes , which can degrade rubber polymers. To achieve our goal, we will use a combination of three enzymes: a laccase, a lipoxygenase or latex clearing protein (LCP) and a cytochrome C. The laccase known as an oxidoreductase will oxidize the cytochrome C which will be previously light excited. Coupled with this later, the LCP will degrade synthetics polymers found in the chewing gum.
 
−
We know that the cytochrome C needs to be maturated and to incorporate a heme into its structure. To optimize the function of the cytochrome C, we will express the gene cluster involved in the cytochrome C maturation and a gene to produce a heme.
 
−
In order to have the best chance of success, we selected different enzymes: 4 laccases, 2 LCP and 2 cytochromes C and we will try many combinations.
 
−
Moreover, we would like to fuse laccases and cytochromes C. But, we were stunned by the lack of information about linker designing. Therefore, we will try to create a tool to easily design linker to make fusion proteins.
 
−
Once several combinations will be properly cloned into an E.coli strain, we will purify proteins. Then, we will perform assays to test their enzymatic activities by oxygen measurement and spectrophotometry.
 
−
</div>
 
  
 +
    </section>
 +
    <!-- end hearo section -->
 +
 +
    <div class="clearfix"></div>
 +
      <!-- start project section -->
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    <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
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    <div class="col-md-6 left">
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    <h2 class="title wow bounceInUp"><span style="color:#8E3B8C"><span style="font-family:Armalite rifle">OUR IDEA</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style="font-family:Courier New">Nowadays, chewing gum is the second urban pollutant after cigarette butts. To clean chewing gum, specific engines are used but they are heavy and expensive. The time required for its use is long and they need a large quantity of water. Besides economical aspects, chewing gums have an environmental impact. They are dangerous for the wildlife such as birds. Indeed, believing that it is bread, they eat chewing gums and choke. Regarding these alarming facts, we have decided to create a new environmentally- responsible way to sanitize our streets.</div></p></span></span>
 +
 +
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
                    <div class="success-work project">
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                        <div class="success-work-desc">
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                            <img src="https://static.igem.org/mediawiki/2015/3/38/PIm24O7.jpg" class="img-responsive" width="400" height="250">
 +
                         
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                            <a title="Lightbox Example"  href=https://2015.igem.org/Team:Aix-Marseille/Project/Description" class="btn outlinebtn  space10">
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                            Details !
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                          </a>
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                        </div>
 +
                       
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                    </div>
 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 +
    </section>
 +
    <div class="clearfix"></div>
 +
      <!-- start science section -->
 +
<section style="padding:30px 0px 50px;background-color: #fbf1fb;" "background:#fbf1fb;"="" class="bg-2 arrow_box2;">
 +
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    <div style ="background:#FBF1FB;">
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    <div class="container">
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    <div class="col-md-6 left">
 +
    <h2 class="title wow bounceInUp"><span style ="color:#000000"><span style="font-family:Armalite rifle">OUR PROJECT</span></h2></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Our project is to create an <I>E.coli</I> strain that produces enzymes , which can degrade rubber polymers. To achieve our goal, we will use a combination of three enzymes: a laccase, a lipoxygenase or latex clearing protein (LCP) and a cytochrome C. The laccase known as an oxidoreductase will oxidize the cytochrome C which will be previously light excited. Coupled with this later, the LCP will degrade synthetics polymers found in the chewing gum. </p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">We know that the cytochrome C needs to be maturated and to incorporate a heme into its structure. To optimize the function of the cytochrome C, we will express the gene cluster involved in the cytochrome C maturation and a gene to produce a heme.</p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">In order to have the best chance of success, we selected different enzymes: 4 laccases, 2 LCP and 2 cytochromes C and we will try many combinations.</p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Moreover, we would like to fuse laccases and cytochromes C. But, we were stunned by the lack of information about linker designing. Therefore, we will try to create a tool to easily design linker to make fusion proteins. </p></span></div></span>
 +
<p><div align="justify"><span style ="color:#000000"><span style="font-family:Courier New">Once several combinations will be properly cloned into an E.coli strain, we will purify proteins. Then, we will perform assays to test their enzymatic activities by oxygen measurement and spectrophotometry.
 +
</p></span></div></span>
 +
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1 space30 text-center">
 +
                    <div class="success-work science">
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                        <div class="success-work-desc">
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                        <img src="https://static.igem.org/mediawiki/2015/2/2b/WJg8VPU.jpg" class="img-responsive" space200>
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                            Details!
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                        </div>
 +
                        </div>
 +
                    </div>
 +
   
 +
    </div>
 +
    </div>
 +
    </div>
 +
 +
    </section>
 +
   
 +
 +
    </section>   
 +
      <!-- start social section -->
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    <section style="padding:50px 0px;" class="arrow_box" id="ethics">
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<h1 class="blue">MORE INFORMATION ON FACEBOOK !</h1>
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 +
 +
    </section>
 +
      <div class="clearfix"></div>
 +
      <!-- start sponsors section -->
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                    <h2 class="gray">SPONSORS</h2>
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    class="img-grey mautomargin"></a></li>
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  <li><a href="http://www.univ-amu.fr/">
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    <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"
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    class="img-grey mautomargin" width="200" align="center" ></a></li>
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  <li><a href="http://polytech.univ-amu.fr/">
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    <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"
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    class="img-grey mautomargin" width="200" align="center"></a></li>
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  <li><a href="http://www.grandluminy.com/">
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  <li><a href="https://www.eppendorf.com/FR-fr/">
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    class="img-grey mautomargin"></a></li>
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                        <p class="fsize18 space10">Chew figth project, for the iGEM competition.</p>
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Latest revision as of 23:50, 18 September 2015

Chew fight

NO MORE GUM ON OUR STREETS

OUR IDEA

Nowadays, chewing gum is the second urban pollutant after cigarette butts. To clean chewing gum, specific engines are used but they are heavy and expensive. The time required for its use is long and they need a large quantity of water. Besides economical aspects, chewing gums have an environmental impact. They are dangerous for the wildlife such as birds. Indeed, believing that it is bread, they eat chewing gums and choke. Regarding these alarming facts, we have decided to create a new environmentally- responsible way to sanitize our streets.

Details !

OUR PROJECT

Our project is to create an E.coli strain that produces enzymes , which can degrade rubber polymers. To achieve our goal, we will use a combination of three enzymes: a laccase, a lipoxygenase or latex clearing protein (LCP) and a cytochrome C. The laccase known as an oxidoreductase will oxidize the cytochrome C which will be previously light excited. Coupled with this later, the LCP will degrade synthetics polymers found in the chewing gum. 

We know that the cytochrome C needs to be maturated and to incorporate a heme into its structure. To optimize the function of the cytochrome C, we will express the gene cluster involved in the cytochrome C maturation and a gene to produce a heme.

In order to have the best chance of success, we selected different enzymes: 4 laccases, 2 LCP and 2 cytochromes C and we will try many combinations.

Moreover, we would like to fuse laccases and cytochromes C. But, we were stunned by the lack of information about linker designing. Therefore, we will try to create a tool to easily design linker to make fusion proteins. 

Once several combinations will be properly cloned into an E.coli strain, we will purify proteins. Then, we will perform assays to test their enzymatic activities by oxygen measurement and spectrophotometry.

Details!

MORE INFORMATION ON FACEBOOK !

SPONSORS

Useful Links

Contact Us

  • MAIL

  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition.