Difference between revisions of "Team:Evry/Project/Biosensor"

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<p class="text-justify">We have a way to detect hypoxia area to locate tumor cells, now it’s time to destroy them.
 
<p class="text-justify">We have a way to detect hypoxia area to locate tumor cells, now it’s time to destroy them.
 
In order to kill hypoxic tumor cells, we created a yeast secretion system for perforin and granzyme B under control of our hypoxia bio-sensor. Perforin is a pore-forming enzyme, allowing granzyme B to enter the cell. Granzyme B is a serine protease capable of inducing cell apoptosis by caspase activation. Co-expressed, these enzymes have proven their potential to induce cancer cell death :</p>
 
In order to kill hypoxic tumor cells, we created a yeast secretion system for perforin and granzyme B under control of our hypoxia bio-sensor. Perforin is a pore-forming enzyme, allowing granzyme B to enter the cell. Granzyme B is a serine protease capable of inducing cell apoptosis by caspase activation. Co-expressed, these enzymes have proven their potential to induce cancer cell death :</p>
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<ul>
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<il><strong>A.</strong> In vitro delivery of granzyme B to colon cancer cells with perforin resulted in caspase 3 activation and features of apoptosis in those cells : chromatin condensation, nucleus fragmentation and internucleosomal DNA fragmentation (8)</il>
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<il><strong>B.</strong> Recombinant Granzyme B and perforin co-expressed in E. coli induced apoptosis and directly inhibited the growth of human laryngeal cancer Hep-2 cells in vitro (9)</il>
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</ul>
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Revision as of 00:01, 19 September 2015

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