Difference between revisions of "Team:NCTU Formosa/Notebook"

 
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{{Team:NCTU_Formosa/navigation}}
 
{{Team:NCTU_Formosa/navigation}}
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<body>
 
<body>
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</div>
 
</div>
 
<div class="p02">
 
<div class="p02">
−
<div class="contentitle">
+
<div class="abgne_tab">
−
Single chain variable fragment as probe
+
<ul class="tabs">
−
</div>
+
<li><a href="#tab2">2015 April</a></li>
 +
<li><a href="#tab3">2015 May</a></li>
 +
<li><a href="#tab4">2015 June</a></li>
 +
<li><a href="#tab5">2015 July</a></li>
 +
<li><a href="#tab6">2015 August</a></li>
 +
</ul>
 +
 +
<div class="tab_container">
 +
<div id="tab2" class="tab_content">
 +
<div class="CSSTableGenerator" >
 +
                <table >
 +
                    <tr>
 +
                        <td>
 +
                          Sun.
 +
                        </td>
 +
                        <td >
 +
                            Mon.
 +
                        </td>
 +
                        <td>
 +
                            Tue.
 +
                        </td>
 +
<td>
 +
                          Wed.
 +
                        </td>
 +
                        <td >
 +
                            Thu.
 +
                        </td>
 +
                        <td>
 +
                            Fri.
 +
                        </td>
 +
<td>
 +
                            Set.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
<td >
 +
                          <B>1</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                          <B>2</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>3</B><br><br>
 +
                        </td>
 +
<td>
 +
                            <B>4</B><br><br>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                          <B>5</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                          <B>6</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                          <B>7</B><br><br>
 +
                        </td>
 +
<td >
 +
                          <B>8</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>9</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>10</B><br><br>
 +
                        </td>
 +
<td >
 +
                            <B>11</B><br><br>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                            <B>12</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>13</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>14</B><br><br>
 +
                        </td>
 +
<td >
 +
                            <B>15</B><br><br>
 +
<b>Transformation of</b><br>GBP[FadL-GBP]<br>
 +
Cultivation of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 +
                        </td>
 +
                        <td>
 +
                            <B>16</B><br><br>
 +
Cultivation of <br>GBP[FadL-GBP]
 +
<br>
 +
<b>Plasmid purification of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
  
−
<div class="content">
+
                        </td>
−
    Single chain variable fragment (scFv) Abs are one of the <font color=#b51c48> recombinant antibody(rAb)</font> fragments, which are popular therapeutic alternatives to full length of monoclonal Abs. Compared to generating whole Abs from animal cell culture, scFv are smaller and can be expressed rapidly, economically and in large quantities in a bacterial host, such as<font color=#b51c48> E. coli</font>. A scFv <font color=#b51c48>possesses the complete antigen binding site</font>, which contains the variable heavy (VH) and variable light domain of an antibody. The VH domain is linked to a VL domain by an introduced flexible polypeptide linker. A scFv is capable of binding its target antigens with an affinity similar to that of the parent mAb. Due to containing the specific antigen binding unit, scFv fragments show tremendous versatility and importance in<font color=#b51c48> human therapeutics and diagnostics</font>. [1] In addition, scFv fragments can be envisaged to be applied in the non-pharmaceutical sector, such as in the food, cosmetic or environmental industries. The unique and highly specific antigen-binding ability might, for example, be exploited to block specific enzymes (e.g. enzymes that cause food spoilage), bacteria (e.g. in toothpaste or mouthwashes) or to detect environmental factors present in very low concentrations (as biosensors).[2]
+
                        <td>
 +
                            <B>17</B><br><br><b>Plasmid purification of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[FadL-GBP]  (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>GBP[FadL-GBP] (EcoRI and PstI)
  
 +
                        </td>
 +
<td >
 +
                          <B>18</B><br><br>
 +
  <b>Transformation of</b><br>ligated product:
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                            <B>19</B><br><br>
 +
<b>Cultivation of</b>
 +
<br>ligated product: GBP[FadL-GBP] C<sup>r</sup>
 +
<br>PCR product: Terminator[J61048]
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>20</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>21</B><br><br>
 +
<b>Cultivation of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>22</B><br><br>
 +
<b>Plasmid purification of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+PSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>23</B><br><br>
 +
<b>Digestion of</b>
 +
OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>24</B><br><br>
 +
<b>Digestion of</b>
 +
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
 +
 +
                        </td>
 +
<td >
 +
                            <B>25</B><br><br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Digestion of</b>
 +
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 +
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 +
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[GBP+B0034+pSB1K3] K<sup>r</sup>
 +
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
<tr>
 +
                        <td >
 +
                            <B>26</B><br><br>
 +
<b>Ligation of</b>
 +
<br>RFP[E1010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation</b><br>of ligated product:
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+B0034+pSB1K3] K<sup>r</sup>
 +
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b><br>digested product:
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>[J23101+B0034]
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>27</B><br><br>
 +
  <b>Digestion of</b>
 +
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>PCR of</b><br>ligated product:
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>28</B><br><br>
 +
<b>Electrophoresis of</b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
 +
<b>Transformation of</b><br>ligated product:
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 +
<b>PCR of</b><br>ligated product
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>29</B><br><br>
 +
<b>Cultivation of</b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
 +
<b>PCR of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
<br><b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+J61048+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>30</B><br><br>
 +
  <b>Plasmid purification of</b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
<b>PCR of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
<b>Transformation of</b>
 +
<br>mini: Terminator[J61048]
 +
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
</div>
 +
<div id="tab3" class="tab_content">
 +
<div class="CSSTableGenerator" >
 +
                <table >
 +
                    <tr>
 +
                        <td>
 +
                          Sun.
 +
                        </td>
 +
                        <td >
 +
                            Mon.
 +
                        </td>
 +
                        <td>
 +
                            Tue.
 +
                        </td>
 +
<td>
 +
                          Wed.
 +
                        </td>
 +
                        <td >
 +
                            Thu.
 +
                        </td>
 +
                        <td>
 +
                            Fri.
 +
                        </td>
 +
<td>
 +
                            Set.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                           
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
<td >
 +
                         
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
                        <td>
 +
                            <B>1</B><br><br><b>PCR of</b><br>Transformation product:
 +
<br>mini: Terminator[J61048]
 +
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>mini: Terminator[J61048]
 +
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
 +
                        </td>
 +
<td>
 +
                            <B>2</B><br><br>
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                          <B>3</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>4</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
5/4
 +
<b>Transformation off</b> ligated products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
 +
<br>
 +
<b>PCR of</b> ligated products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>5</B><br><br><b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP+J61048] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b> PCR product:
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>6</B><br><br><b>Plasmid purification of</b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b> PCR product:
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b> backbone:
 +
<br>[pSB1C3] C<sup>r</sup>
 +
<br>[pSB1A3] A<sup>r</sup>
 +
<br>[pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>7</B><br><br><b>Digestion of </b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>[pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>8</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transformation off</b> ligated products:
 +
<br>RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>9</B><br><br><b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                            <B>10</B><br><br><b>Electrophoresis of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>
 +
<br><b>Plasmid purification of</b>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[B0030] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>[B0030] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>11</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>12</B><br><br><b>PCR of</b> ligated products:
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b> PCR products:
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>PCR products: RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>13</B><br><br><b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>14</B><br><br><b>Cultivation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>15</B><br><br><b>Digestion of </b>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>16</B><br><br><b>Transformation off</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td >
 +
                            <B>17</B><br><br><b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>18</B><br><br><b>Cultivation of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GFP[B0030+E0040+J61048]
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>19</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>20</B><br><br><b>Cultivation of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and Pst1)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>[pSB3C5] 抗性
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>21</B><br><br><b>Digestion of </b>
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>22</B><br><br><b>Cultivation of</b>
 +
<br>[pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>23</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
<tr>
 +
                        <td >
 +
                            <B>24</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23101+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>25</B><br><br><b>Transformation off</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[B003+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>26</B><br><br><b>Ligation of</b>
 +
<br>OmpA[J23110+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
 +
 +
                        </td>
 +
<td >
 +
                            <B>27</B><br><br><b>PCR of</b>
 +
<br>[J23101+B0034+OmpA+pSB3C5]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>28</B><br><br><b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性??
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>[J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S)
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<br>Plasmid purification in gel slices of PCR products:
 +
<br>OmpA[EX-OmpA-S]
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>OmpA[OmpA] (EcoRI and SpeI)
 +
<br>
 +
<b>Transformation off</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5]
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>29</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>30</B><br><br><b>PCR of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
<tr>
 +
                        <td >
 +
                            <B>31</B><br><br><b>Cultivation of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
</div>
 +
<div id="tab4" class="tab_content">
 +
<div class="CSSTableGenerator" >
 +
                <table >
 +
                    <tr>
 +
                        <td>
 +
                          Sun.
 +
                        </td>
 +
                        <td >
 +
                            Mon.
 +
                        </td>
 +
                        <td>
 +
                            Tue.
 +
                        </td>
 +
<td>
 +
                          Wed.
 +
                        </td>
 +
                        <td >
 +
                            Thu.
 +
                        </td>
 +
                        <td>
 +
                            Fri.
 +
                        </td>
 +
<td>
 +
                            Set.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
<td>
 +
</td>
 +
                        <td >
 +
                            <B>1</B><br><br><b>Electrophoresis of</b>
 +
<br>[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>GFP[K1489002+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>[pSB3C5] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GFP[J23101+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
 +
<br>GFP[J23110+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
 +
<br>
 +
<b>Electrophoresis of</b> digested product:
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[OmpA+K592100]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>2</B><br><br><b>PCR of</b>
 +
<br>OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1A3] (EcoRI and NcoI)
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>[pSB1A3] (EcoRI and NcoI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>3</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA+psB1C3] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA+psB1C3] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+ K592100] (NcoI and PstI)
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
 +
                        </td>
 +
<td >
 +
                          <B>4</B><br><br><b>Cultivation of</b>
 +
<br>[J23110+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>5</B><br><br><b>Transform of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3]
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>6</B><br><br><b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
 +
                        </td>
 +
 +
                    </tr>
 +
                    <tr>
 +
<td>
 +
                            <B>7</B><br><br><b>Gel extraction of</b>
 +
<br>OmpA[EX-OmpA-S] 抗性
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
 +
                        </td>
 +
                        <td >
 +
                          <B>8</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3K3]
 +
[<br>pSB6A1]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[EX-OmpA-S] (EcoRI and SpeI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>9</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB3K3]
 +
<br>[pSB6A1]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3K3] (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>10</B><br><br><b>Transform of</b>
 +
<br>[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23101+B0034+OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3K3] (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3K3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>11</B><br><br><b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>12</B><br><br><b>PCR of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>13</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
</tr>
 +
 +
                 
 +
<tr><td >
 +
                            <B>14</B><br><br><b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB3C5]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B]
 +
<br>scFv-C[OmpA+scFv-C]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110]
 +
<br>[B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td >
 +
                            <B>15</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>16</B><br><br><b>Transform of</b>
 +
<br>[pSB3C5]
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>17</B><br><br><b>Cultivation of</b>
 +
<br>[pSB3C5]
 +
 +
                        </td>
 +
<td >
 +
                            <B>18</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3C5]( EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3C5]( EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>19</B><br><br><b>Transform of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>20</B><br><br><b>PCR of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
 +
                    </tr>
 +
                    <tr><td >
 +
                          <B>21</B><br><br>
 +
                        </td>
 +
                        <td >
 +
                            <B>22</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                          <B>23</B><br><br><b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB3C5]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>BFP[OmpA+K592100]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1]
 +
<br>[psB6A1]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>24</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5]
 +
 +
                        </td>
 +
<td >
 +
                            <B>25</B><br><br><b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>26</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>27</B><br><br><b>Digestion of </b>
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] 抗性 (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
 +
                    </tr>
 +
<tr><td >
 +
                            <B>28</B><br><br><b>Cultivation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>[pSB3C5]
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23101+B0034] K<sup>r</sup>
 +
<br>GBP[FadL-GBP] 抗性 (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[J23101+B0034+OmpA+K592100] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
<br>[E0040] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>[B0034+E0040+pSB1K3] K<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1A3]
 +
<br>[pSB1C3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3C5]
 +
<br>[pSB6A1]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td >
 +
                            <B>29</B><br><br><b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>30</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                         
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
</div>
 +
<div id="tab5" class="tab_content">
 +
<div class="CSSTableGenerator" >
 +
                <table >
 +
                    <tr>
 +
                        <td>
 +
                          Sun.
 +
                        </td>
 +
                        <td >
 +
                            Mon.
 +
                        </td>
 +
                        <td>
 +
                            Tue.
 +
                        </td>
 +
<td>
 +
                          Wed.
 +
                        </td>
 +
                        <td >
 +
                            Thu.
 +
                        </td>
 +
                        <td>
 +
                            Fri.
 +
                        </td>
 +
<td>
 +
                            Set.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
                        <td >
 +
                            <B>1</B><br><br><b>Cultivation of</b>
 +
<br>[pSB6A1]
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[]FadL-GBP] 抗性 (EcoRI and PstI)
 +
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>2</B><br><br><b>Digestion of </b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>
 +
<br>Plasmid purification in gel slices of
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>3</B><br><br><b>Gel extraction of</b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] (EcoRI and Pstl)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>4</B><br><br><b>Transform of</b>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
 +
                        </td>
 +
                       
 +
                       
 +
                    </tr>
 +
                    <tr><td>
 +
                          <B>5</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td><td>
 +
                            <B>6</B><br><br><b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
 +
                        </td>
 +
<td>
 +
                            <B>7</B><br><br><b>Digestion of </b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td >
 +
                          <B>8</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>9</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3C5]
 +
<br>BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>10</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>11</B><br><br><b>PCR of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
 +
                        </td>
 +
                       
 +
                    </tr>
 +
                    <tr><td>
 +
                            <B>12</B><br><br><b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>13</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
<td >
 +
                            <B>14</B><br><br><b>Digestion of </b>
 +
<br>[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<br><b>Electrophoresis of</b>
 +
<br>[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td >
 +
                            <B>15</B><br><br><b>Digestion of </b>
 +
<br>[pSB6A1]
 +
<br>[pSB3C5]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>[J23101+B0034+OmpA+scFv-C]
 +
<br>[J23101+B0034+OmpA+scFv-B]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB6A1]
 +
<br>[R0010]
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>16</B><br><br><b>Gel extraction of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>17</B><br><br><b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin+E1010] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
<td >
 +
                            <B>18</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                       
 +
                       
 +
                    </tr>
 +
                    <tr><td>
 +
                            <B>19</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 +
                        </td><td>
 +
                            <B>20</B><br><br>
 +
                        </td>
 +
<td >
 +
                          <B>21</B><br><br>
 +
                        </td>
 +
                        <td >
 +
                            <B>22</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                          <B>23</B><br><br>
 +
                        </td>
 +
                        <td>
 +
                            <B>24</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>25</B><br><br><b>Cultivation of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 +
                        </td>
 +
                       
 +
                       
 +
                    </tr>
 +
<tr><td>
 +
                            <B>26</B><br><br><b>Plasmid purification of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[R0010] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[K592100+J61048] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
 +
                        </td><td>
 +
                            <B>27</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3]
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>[pSB6A1]
 +
 +
                        </td>
 +
<td >
 +
                            <B>28</B><br><br><b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
 +
<br>OmpA[J23110+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA-linker] C<sup>r</sup>
 +
 +
                        </td>
 +
                        <td >
 +
                            <B>29</B><br><br><b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5]
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
 +
<br>GBP[GBP] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
 +
<br>GBP[GBP] (EcoRI and PstI)
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>30</B><br><br><b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB1C3]C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin own]  (NcoI and PstI)
 +
<br>[pSB1A3]  (EcoRI and PstI)
 +
<br>[pSB1C3]  (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>[J23101+B0034] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin own]  (NcoI and PstI)
 +
<br>[pSB1A3]  (EcoRI and PstI)
 +
<br>[pSB1C3]  (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>[J231-1+B0034] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>31</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                       
 +
                     
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
</div>
 +
<div id="tab6" class="tab_content">
 +
<div class="CSSTableGenerator" >
 +
                <table >
 +
                    <tr>
 +
                        <td>
 +
                          Sun.
 +
                        </td>
 +
                        <td >
 +
                            Mon.
 +
                        </td>
 +
                        <td>
 +
                            Tue.
 +
                        </td>
 +
<td>
 +
                          Wed.
 +
                        </td>
 +
                        <td >
 +
                            Thu.
 +
                        </td>
 +
                        <td>
 +
                            Fri.
 +
                        </td>
 +
<td>
 +
                            Set.
 +
                        </td>
 +
                    </tr>
 +
<tr>
 +
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td>
 +
</td>
 +
<td >
 +
                            <B>1</B><br><br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
 +
                        </td></tr>
 +
                    <tr>
 +
                       
 +
                        <td>
 +
                            <B>2</B><br><br><b>Electrophoresis of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and PstI)
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>3</B><br><br><b>Transform of</b>
 +
[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                          <B>4</B><br><br><b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>5</B><br><br><b>Digestion of </b>
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+oSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP]C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[OmpA] (NcoI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>6</B><br><br><b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>[pSB1K3]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>BFP[B0030+K592100+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[B0030+K592100+J61048]  (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034] K<sup>r</sup>
 +
<br>GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[scFv-C] C<sup>r</sup>
 +
<br>scFv-B[scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>[B0034]
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>[pSB6A1] (XbaI and PstI)
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 +
                        </td>
 +
<td>
 +
                            <B>7</B><br><br><b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 +
                        </td> <td >
 +
                          <B>8</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1K3]
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA] C<sup>r</sup> NcoI and PstI
 +
<br>OmpA[OmpA+K592100] C<sup>r</sup> NcoI and PstI
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-Own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                     
 +
                        <td>
 +
                          <B>9</B><br><br><b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>[pSB1K3]
 +
<br>[pSB1A3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (NcoI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[GBp+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                          <B>10</B><br><br><b>Electrophoresis of</b>
 +
<br>GBP[GBp+pSB1C3] C<sup>r</sup>
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and Pstl)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web]
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>Anti-Her 2[Herceptin-Own]
 +
 +
                        </td>
 +
<td >
 +
                          <B>11</B><br><br><b>Plasmid purification of</b>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<br><b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b><b><b>Digestion of </b> </b></b>
 +
<br>[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>12</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>13</B><br><br><b>Transform of</b>
 +
<br>GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
 +
                        </td>
 +
<td >
 +
                            <B>14</B><br><br><b>Transform of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td><td >
 +
                            <B>15</B><br><br><b>PCR of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] (EcoRI and PstI)
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                       
 +
                        <td>
 +
                            <B>16</B><br><br><b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>OmpA[R0010+B0034+OmpA]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>17</B><br><br><b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>18</B><br><br><b>Plasmid purification of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>19</B><br><br><b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C]
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[R0010+B034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br><b>PCR of</b>
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B]
 +
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>20</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP] K<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>OmpA[OmpA] (EcoRI and SpeI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] K<sup>r</sup>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup> 
 +
<br>GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048]
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup> 
 +
<br>GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] A<sup>r</sup> 
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br><b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040]
 +
 +
 +
 +
                        </td>
 +
<td >
 +
                          <B>21</B><br><br>
 +
  <b>Electrophoresis of</b>
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[R0010] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J23110] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0034+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>RFP[B0034+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[B0034+FadL-GBP+B0030+J61048] C<sup>r</sup> (XbaI and PstI)
 +
 +
<br><b>Ligation of</b>
 +
<br>BFP[J23110+B0034+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+K592100+E1010+J61048pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3]
 +
 +
<br><b>Transform of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048pSB1A3] A<sup>r</sup> 
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
<br>GBP[J23101+B0034+FadL-GBP]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040]
 +
 +
                        </td><td >
 +
                            <B>22</B><br><br>
 +
<b>Digestion of</b>
 +
<br>[pSB1C3] (XbaI and SpeI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[J23110+B0034+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[FadL-GBP] (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Digestion of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E0040+J61048]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+K592100+J61048]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                       
 +
                        <td>
 +
                          <B>23</B><br><br>
 +
  <b>Transform of</b>
 +
<br>GBP[FadL-GBP] A<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>BFP[J23101+B0034+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own]
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>BFP[J23101+B0034+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>GBP[J23110+B0034+fadL-GBP+B0030+E0040]
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and SpeI)
 +
<br>GBP[B0034+FadL-GBP+B0030+E0040+J61048] (XbaI and PstI)
 +
<br>RFP[B0030+E1010+J61048] (XbaI and PstI)
 +
<br>BFP[J23101+B0034+K592100+J61048]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web] (EcoRI and PstI)
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own] (EcoRI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] (EcoRI and PstI)
 +
<br>GBP[R0010+B0034FadL-GBP] (EcoRI and PstI)
 +
<br>[pSB1C3] (XbaI and PstI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>24</B><br><br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b>
 +
<br>Anti-Her 2[Herceptin-own] (NcoI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
 +
<br>GBP[FadL-GBP] (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[J23110+B0034+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>GFP[J23110+B0034+E0040+J61048
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>[pSB1C3]
 +
<br>OmpA[R0010+B0034+OmpA+J61048]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>OmpA[OmpA]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+] C<sup>r</sup>
 +
 +
                        </td>
 +
<td >
 +
                            <B>25</B><br><br><b>Digestion of</b>
 +
<br>[pSB3C5] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[OmpA+scFv-B]
 +
<br>Anti-Her 2[OmpA+Herceptin-own]
 +
<br>GBP[FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B +B0030+E0040+J61048]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]  (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>BFP[R0010+B0034+OmpA+K592100] (NcoI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] A<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>OmpA[R0010+B0034+OmpA]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>BFP[OmpA+K592100] (EcoRI and NcoI)
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>26</B><br><br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[FadL-GBP] (XbaI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+K592100]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>[J23101] (EcoRI and SpeI)
 +
<br>BFP[OmpA+K592100] (EcoRI and NcoI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>BFP[R0010+B0034+OmpA+K592100]
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] (EcoRI and PstI)
 +
<br>BFP[R0010+B0034+OmpA+K592100] (EcoRI and PstI)
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>(EcoRI and PstI I)
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3]
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>27</B><br><br><b>Transform of</b>
 +
<br>GBP[FadL-LGBP] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>scFv-B [R0010+B0034+OmpA+scFv-B]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GFP[B0034+E0040+J61048] (XbaI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GFP[J23110+B0034+E0040+J61048] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>GFP[J23110+B0034+E0040+pSB6A1]
 +
 +
                        </td>
 +
<td >
 +
                            <B>28</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E1010+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+K592100+J61048]
 +
<br>Anti-Her 2[OmpA+Herceptin-own]
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own]
 +
<br>scFv-C[OmpA+scFv-C].
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own]
 +
<br>BFP[B0030+K592100+J61084]
 +
<br>BFP[B0030+K592100+J61084]
 +
<br>BFP[J23101+B0034+OmpA+K592100]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E0040+J61048]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030]
 +
 +
                        </td><td >
 +
                            <B>29</B><br><br>
 +
                        </td>
 +
                    </tr>
 +
<tr>
 +
                       
 +
                        <td>
 +
                          <B>30</B><br><br><b>Plasmid purification of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
 +
                        </td>
 +
                        <td>
 +
                            <B>31</B><br><br>
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
                        <td>
 +
                           
 +
                        </td>
 +
<td >
 +
                           
 +
                        </td>
 +
<td>
 +
</td>
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
 +
<div class="goto">
 +
<a href="https://2015.igem.org/Team:NCTU_Formosa/Project"><img src="https://static.igem.org/mediawiki/2015/3/3c/%E7%AE%AD%E9%A0%AD1.png"; width=50vw;><br><br>Back to Navigation</a>
 
</div>
 
</div>
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<div class="contentitle">Properties and development of targeted drugs</div>
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<div class="goto1">
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<div class="content">
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<a href="https://2015.igem.org/Team:NCTU_Formosa/Protocol"><img src="https://static.igem.org/mediawiki/2015/c/c2/%E7%AE%AD%E9%A0%AD2.png"; width=50vw;><br><br>Go to Protocol</a>
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This year, we decided to utilize the scFv as probes to detect cancer markers and aid in the prescription of targeted drugs in cancer treatments.
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Targeted drugs therapy utilize compounds that are capable of inhibiting target molecules, the cancer markers which send messages along signaling pathways in cell growth, cell division or cell death. Via specific binding to target molecules, targeted drugs show more accurate attack to cancer cells and less harmful damage to normal tissues. [1] The precision of targeting the cancer cells has enhanced the efficiency of treatment by a large margin. The targeted therapy is a major step forward for many cancers, especially advanced cancers, and physicians and researchers are now focusing on the development of targeted drugs, creating a new era of personalized cancer treatment.[3]Targeted therapy are so-called "personalized medicine" because health care professionals can use clinical test results from a patient to select a specific drug that has a higher likelihood of being effective for that particular person.<br><br>
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According to the statistics, the usage rate of targeted drug therapy has increased within ten years. In Figure 1, in 2003, targeted drug therapy is not commonly used compared with other therapies, accounting for only 11% usage. Over one decade, it is estimated that the usage of targeted drug therapy dramatically increases to<font color=#b51c48> 46%</font>. It indicates targeted drugs therapy is a potential growing field and will become the commonly used therapy in cancer treatments in the near future.
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<br><br>
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<div class="contentitle">Pre-diagnosis of targeted drugs treatment</div>
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<div class="content">
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To create the new era of tailored targeted drugs, doctors must aim at<font color=#b51c48> appropriate target molecules </font>for patients with particular diseases. In 2014,<font color=#b51c48> the U.S. Food and Drug Administration (FDA) </font>issued a guidance to facilitate the development and review of <font color=#b51c48>diagnostics tests</font>. The diagnostics tests are the steps to identify the abnormal cancer biomarkers. Moreover, the purpose of diagnostics tests are to help medical practitioners <font color=#b51c48>determine which patients could benefit from the certain drugs</font>, conversely, those who should not receive the medication. If the treatment decisions is not optimal, it would not only cause the fatal body damage, but also lead to the waste of time, money and medical resources. FDA encourages the joint of targeted drugs therapies and precise diagnostics tests which are essential for the safe and effective use of targeted drugs.[4]
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</div>
 
</div>
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<div class="contentitle">The concept of combination therapy</div>
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<div class="content">
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Although targeted drugs treatments can lead to the dramatic regressions of solid tumors, the responses are often short-lived because resistant cancer cells arise after a period of treatment. The major strategy proposed for overcoming the resistance is <font color=#b51c48>combination therapy</font>. The clinical and preclinical researches further indicated that targeted drug therapy combined with another targeted drug therapy or other types of therapies to treat cancers simultaneously may attain greater effects than using only one therapy. With the concept of combination therapy, we can not only improve the treating effect but also reduce the occurrence of cancer cells resistance toward the targeted drugs as there are less probability that a single mutation will cause cross-resistance to both drugs.[2] </div>
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<div class="contentitle">APPOllO E.Cotector</div>
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<div class="content">To enhance the <font color=#b51c48>efficiency of diagnosis </font>and provide reference for<font color=#b51c48> proper usage of targeted drugs</font> and <font color=#b51c48>combination therapy</font>, we come up with the idea of detecting multimarker at the same time and this was how our marvelous E.Cotector is borned. This year, NCTU_Formosa commits to creating a multimarker diagnosis platform via scFv as probes for helping physicians to determine and prescribe the usage of targeted drugs in cancer patients, especially the monoclonal-antibody-targeted drugs.</div>
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{{Team:NCTU_Formosa/footer}}
 
{{Team:NCTU_Formosa/footer}}

Latest revision as of 00:01, 19 September 2015

Lab note
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

 2

 3

 4
5

 6

 7

 8

 9

 10

 11
12

 13

 14

 15

Transformation of
GBP[FadL-GBP]
Cultivation of
OmpA[OmpA+pSB1K3] Kr 		16

Cultivation of
GBP[FadL-GBP]
Plasmid purification of
OmpA[OmpA+pSB1K3] Kr
Digestion of
OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) 		17

Plasmid purification of
GBP[FadL-GBP]
Digestion of
GBP[FadL-GBP] (EcoRI and PstI)
Electrophoresis of
digested product:
GBP[FadL-GBP] (EcoRI and PstI) 		18

Transformation of
ligated product:
GBP[FadL-GBP+pSB1C3] Cr
19

Cultivation of
ligated product: GBP[FadL-GBP] Cr
PCR product: Terminator[J61048] 		20

 21

Cultivation of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+pSB1C3] Cr 		22

Plasmid purification of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+PSB1C3] Cr
Digestion of
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI)
Electrophoresis of
digested product:
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI) 		23

Digestion of OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI)
Electrophoresis of
digested product:
OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI) 		24

Digestion of
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI) 		25

Electrophoresis of
digested product:
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI)
Digestion of
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Electrophoresis of
digested product:
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
26

Ligation of
RFP[E1010+B0034+pSB1K3] Kr
Transformation
of ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[FadL-GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
Electrophoresis of
digested product:
RFP[E1010+J61048+pSB1K3] Kr
[J23101+B0034] 		27

Digestion of
Terminator[J61048] Ar (XbaI and PstI)
PCR of
ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr 		28

Electrophoresis of
RFP[E1010+J61048+pSB1K3] Kr
OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of
ligated product:
GBP[B0034+FadL-GBP+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr PCR of
ligated product
OmpA[J23101+B0034+OmpA] Ar 		29

Cultivation of
RFP[E1010+J61048+pSB1K3] Kr PCR of
OmpA[J23101+B0034+OmpA] Ar
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar Ligation of
GBP[FadL-GBP+J61048+pSB1K3] Kr 		30

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr Ligation of
OmpA[J23101+B0034+OmpA] Ar PCR of
OmpA[J23101+B0034+OmpA] Ar Transformation of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR of
Transformation product:
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
GBP[B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar 		2
3

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		4

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
5/4 Transformation off ligated products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
RFP[E1010+J61048] (Kr) (XbaI and PstI)
PCR of ligated products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		5

Electrophoresis of
digested products:
RFP[E1010+J61048] (Kr) (XbaI and PstI)
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI)
GBP[FadL-GBP+J61048] Kr (EcoRI and PstI)
PCR of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
Cultivation of PCR product:
RFP[E1010+J61048+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		6

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Cultivation of PCR product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
PCR of backbone:
[pSB1C3] Cr
[pSB1A3] Ar
[pSB1K3] Kr
Electrophoresis of
PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] Ar
digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) 		7

Digestion of
RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI)
RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI)
[pSB1C3] Cr (EcoRI and PstI)
[pSB1A3] Ar (EcoRI and PstI)
[pSB1K3] Kr (EcoRI and PstI)
OmpA[J23101+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI)
Plasmid purification of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
[pSB1K3] Kr
Electrophoresis of digested products:
RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI)

RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI)
[pSB1C3] Cr (EcoRI and PstI)
[pSB1A3] Ar (EcoRI and PstI)
[pSB1K3] Kr (EcoRI and PstI)
Ligation of
RFP[B0034+E1010+J61048+pSB1C3] Cr 		8

Electrophoresis of digested products:
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI)
Transformation off ligated products:
RFP[B0034+E1010+J61048+pSB1C3] Cr
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Transformation off
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar 		9

PCR of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar
Cultivation of
[B0030] Ar
[J23110] Ar
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
10

Electrophoresis of
[B0030] Ar
Cultivation of
[B0030] Ar

Plasmid purification of
[J23110] Ar
[B0030] Ar
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
[B0030] Ar (EcoRI and SpeI)
[J23110] Ar (EcoRI and SpeI)
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
Electrophoresis of digested products:
[B0030] Ar (EcoRI and SpeI)
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) 		11

Electrophoresis of digested products:
[J23110] Ar (EcoRI and SpeI)
Ligation of
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr
Transformation off
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr
PCR of
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr 		12

PCR of ligated products:
RFP[B0030+E1010+J61048+pSB1C3] Cr
Cultivation of PCR products:
RFP[B0030+E1010+J61048+pSB1C3] Cr
Plasmid purification of
RFP[B0030+E1010+J61048+pSB1C3] Cr
Digestion of
RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI)
RFP[B0030+E1010+J61048] Cr (EcoRI and PstI)
Electrophoresis of
digested products:
RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI)
RFP[B0030+E1010+J61048] Cr (EcoRI and PstI)
PCR products: RFP[B0030+E1010+J61048+pSB1C3] Cr 		13

Ligation of
[J23110+B0034+pSB1K3] Kr 		14

Cultivation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
Transformation off
[J23110+B0034+pSB1K3] Kr
Electrophoresis of digested products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
[pSB1K3] Kr (EcoRI and PstI) 		15

Digestion of
GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		16

Transformation off
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
Ligation of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Transformation off
[J23110+B0034+pSB1K3] Kr
17

Ligation of
[J23110+B0034+pSB1K3] Kr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr
PCR of
GBP[B0034+FadL-GBP+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of PCR products:
GBP[B0034+FadL-GBP+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr 		18

Cultivation of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Plasmid purification of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Digestion of
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
GFP[B0030+E0040+J61048] Transformation off
[J23110+B0034+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
GFP[B0030+E0040+J61048] 		19

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
digested products:
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Transformation off
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
GFP[B0030+E0040+J61048]
Cultivation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		20

Cultivation of
OmpA[J23101+B0034+OmpA] Ar
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Plasmid purification of
OmpA[J23101+B0034+OmpA] Ar
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Digestion of
OmpA[J23101+B0034+OmpA] Ar (EcoRI and Pst1)
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr (EcoRI and PstI)
OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI)
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Transformation off
[J23110+B0034+pSB1K3] Kr
[pSB3C5] 抗性
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI) 		21

Digestion of
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI)
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Ligation of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr 		22

Cultivation of
[pSB3C5] Cr
[J23110+B0034+pSB1K3] Kr
Transformation off
GBP[J23110+B0034+GBP] Ar
PCR of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Electrophoresis of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Ligation of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
Transform of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar 		23

Plasmid purification of
[pSB3C5] Cr
[J23110+B0034+pSB1K3] Kr
Digestion of
[pSB3C5] Cr (EcoRI and PstI)
[J23110+B0034+pSB1K3] Kr (EcoRI and PstI)
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Transformation off
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
24

Electrophoresis of digested products:
[pSB3C5] Cr (EcoRI and PstI)
[J23110+B0034+pSB1K3] Kr (EcoRI and PstI)
Cultivation of
PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1A3] Ar
Plasmid purification of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
GFP[B0030+E0040+J61048]
Digestion of
[J23101+B0034] Kr (EcoRI and SpeI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
PCR of
GBP[J23110+B0034+GBP] Ar by two pArts
Electrophoresis of
GBP[J23110+B0034+GBP] Ar 		25

Transformation off
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Cultivation of
GBP[J23110+B0034+FadL-GBP] Ar
Digestion of
GBP[B003+FadL-GBP] Cr (XbaI and PstI)
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI)
Electrophoresis of
GFP[B0030+E0040+J61048]
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI)
Cultivation of
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI) 		26

Ligation of
OmpA[J23110+B0034+OmpA+pSB1A3] Ar
GBP[J23110+B0034+GBP+pSB1A3] Ar
Gel extraction of
[J23110] Ar (SpeI and PstI)
Plasmid purification of
GBP[J23110+B0034+GBP] Ar by two pArts
Digestion of
GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI) 		27

PCR of
[J23101+B0034+OmpA+pSB3C5]
Electrophoresis of
GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI)
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
[J23110] Ar (SpeI and PstI) 		28

Ligation of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性??
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
PCR of
[J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S)
OmpA[J23101+B0034+OmpA+pSB3C5] Cr

Plasmid purification in gel slices of PCR products:
OmpA[EX-OmpA-S]
Gel extraction of
OmpA[OmpA] (EcoRI and SpeI)
Transformation off
OmpA[J23101+B0034+OmpA+pSB3C5] 		29

Plasmid purification of
[J23110] Ar
[pSB1C3]
Digestion of
[J23110] Ar (EcoRI and SpeI)
[pSB1C3] (EcoRI and SpeI)
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
Electrophoresis of
[J23110] Ar (EcoRI and SpeI)
[pSB1C3] (EcoRI and SpeI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr 		30

PCR of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
31

Cultivation of
[J23110+B0034] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
Plasmid purification of
[J23110+B0034] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
Digestion of
[J23110+B0034] Kr (EcoRI and PstI)
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI)
[J23110+B0034] (EcoRI and SpeI) Kr
[J23101+B0034] (EcoRI and SpeI) Kr
OmpA[OmpA] Cr (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
Electrophoresis of
[J23110+B0034] Kr (EcoRI and PstI)
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI)
Ligation of
OmpA[OmpA+pSB1C3] Cr
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Electrophoresis of
[J23110+B0034] (EcoRI and SpeI) Kr
[J23101+B0034] (EcoRI and SpeI) Kr
OmpA[OmpA] (NcoI and PstI)
GFP[K1489002+E0040] Kr
Digestion of
[pSB1C3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
OmpA[OmpA] (EcoRI and NcoI)
[pSB3C5] (EcoRI and PstI)
Ligation of
GFP[J23101+B0034+K1489002+E0040+pSB3C5] (Cr)
GFP[J23110+B0034+K1489002+E0040+pSB3C5] (Cr)
Electrophoresis of digested product:
[pSB1C3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
OmpA[OmpA] (EcoRI and NcoI)
Cultivation of
BFP[OmpA+K592100]
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		2

PCR of
OmpA[EX-OmpA-S+pSB1C3] Cr
Electrophoresis of
OmpA[EX-OmpA-S+pSB1C3] Cr
OmpA[OmpA] (EcoRI and NcoI)
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Digestion of
[pSB1A3] (EcoRI and NcoI)
Electrophoresis of digested products:
[pSB1A3] (EcoRI and NcoI) 		3

Ligation of
OmpA[OmpA+psB1C3] Cr
[J23110+B0034+pSB1K3] Kr
Transform of
OmpA[OmpA+psB1C3] Cr
[J23110+B0034+pSB1K3] Kr
Digestion of
BFP[OmpA+ K592100] (NcoI and PstI)
OmpA[OmpA] (NcoI and PstI) 		4

Cultivation of
[J23110+pSB1A3] Ar
Transform of
[pSB1C3]
Digestion of
[pSB1C3] (EcoRI and PstI)
Ligation of
OmpA[OmpA+pSB1C3] Cr
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Transform of
[B0034+pSB1A3] Ar 		5

Transform of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Plasmid purification of
[J23110+pSb1A3] Ar
Digestion of
[J23110+pSb1A3] Ar
PCR of
[J23110+pSb1A3] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Transform of
OmpA[OmpA+pSB1C3] Cr
Electrophoresis of
[pSB1C3]
[B0034+pSB1A3] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		6

Cultivation of
[pSB1C3]
Digestion of
[pSB1C3] (EcoRI and SpeI)
7

Gel extraction of
OmpA[EX-OmpA-S] 抗性
[pSB1C3] (EcoRI and SpeI)
Gel extraction of
[pSB1C3] (EcoRI and SpeI)
Transform of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
[pSB1A3]
OmpA[J23101+B0034+OmpA] Ar
Ligation of
OmpA[OmpA+pSB1C3] Cr
Cultivation of
[pSB1C3] 		8

Transform of
OmpA[OmpA+pSB1C3] Cr
[pSB3K3] [
pSB6A1]
Plasmid purification of
[pSB1C3]
Digestion of
OmpA[EX-OmpA-S] (EcoRI and SpeI)
Electrophoresis of
[pSB1C3] (EcoRI and SpeI)
Transform of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr 		9

Ligation of
OmpA[OmpA+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Cultivation of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Plasmid purification of
[pSB3K3]
[pSB6A1]
Digestion of
[pSB3K3] (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI) 		10

Transform of
[OmpA+pSB1C3] Cr
Plasmid purification of
[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Digestion of
[J23101+B0034+OmpA+K592100] Cr (EcoRI and PstI)
Electrophoresis of
[pSB3K3] (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI)
Transform of
[J23110+B0034] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3]
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Electrophoresis of
[pSB3K3]
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		11

Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
[J23110+B0034+pSB1C3] Cr
Ligation of
[J23110+B0034+pSB1K3] Kr
[J23110+B0034+pSB1C3] Cr
PCR of
OmpA[OmpA+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Electrophoresis of
OmpA[OmpA+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Cultivation of
OmpA[OmpA+pSB1C3] Cr 		12

PCR of
[J23110+B0034+pSB1C3] Cr
[B0034+pSB1A3] Ar
Plasmid purification of
OmpA[OmpA+pSB1C3] Cr
Ligation of
[J23110+B0034+pSB1C3] Cr
Transform of
[J23110+B0034+pSB1C3] Cr 		13

Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
14

SDS-page of
[pSB1C3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
[pSB3C5]
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
scFv-B[OmpA+scFv-B]
scFv-C[OmpA+scFv-C]
Electrophoresis of
[J23110]
[B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI)
Ligation of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Cr
Digestion of
OmpA[OmpA+pSB1C3] Cr (EcoRI and SpeI)
OmpA[OmpA+pSB1C3] Cr (EcoRI and PstI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Ligation of
GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Transform of
GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar 		15

 16

Transform of
[pSB3C5] 		17

Cultivation of
[pSB3C5] 		18

Plasmid purification of
[pSB3C5]
Digestion of
[pSB3C5]( EcoRI and PstI)
Electrophoresis of
[pSB3C5]( EcoRI and PstI)
Ligation of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		19

Transform of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		20

PCR of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
21

 22

 23

PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Cultivation of
[pSB3C5]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
BFP[OmpA+K592100]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1]
[psB6A1]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		24

Plasmid purification of
[pSB3C5] 		25

Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Transform of
[J23101+B0034+pSB1K3] Kr 		26

 27

Digestion of
OmpA[OmpA] Cr (EcoRI and PstI)
Electrophoresis of
GBP[FadL-GBP] 抗性 (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
28

Cultivation of
BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
[pSB3C5]
[pSB1C3]
[pSB1A3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
[J23101+B0034] Kr
GBP[FadL-GBP] 抗性 (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
BFP[J23101+B0034+OmpA+K592100] Ar (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI)
OmpA[OmpA] Cr (XbaI and PstI)
[E0040] Ar (XbaI and PstI)
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
[B0034+E0040+pSB1K3] Kr
GBP[FadL-GBP+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
Digestion of
BFP[J23101+B0034+OmpA+K592100] Cr (XbaI and PstI)
SDS-page of
[pSB1A3]
[pSB1C3]
BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
[pSB3C5]
[pSB6A1]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		29

PCR of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		30

Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Cultivation of
[pSB6A1]
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Digestion of
GBP[]FadL-GBP] 抗性 (EcoRI and PstI) 		2

Digestion of
[J23110] Ar (SpeI and PstI)

Plasmid purification in gel slices of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		3

Gel extraction of
[J23110] Ar (SpeI and PstI)
[pSB6A1] (EcoRI and PstI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
GBP[FadL-GBP] (EcoRI and Pstl)
Ligation of
GBP[FadL-GBP+pSB3C5] Cr
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar 		4

Transform of
GBP[FadL-GBP+pSB3C5] Cr
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
[J23101+B0034+pSB1K3] Kr
5

Plasmid purification of
[J23101+B0034+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		6

Cultivation of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar 		7

Digestion of
[J23101+B0034+pSB1K3] Kr (EcoRI and PstI)
SDS-page of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
Ligation of
[J23110+B0034] Ar
Electrophoresis of
[J23101+B0034+pSB1K3] Kr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		8

Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Transform of
[J23110+B0034] Ar
BFP[J23101+B0034+OmpA+K592100]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100]
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		9

Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] Cr
[pSB3C5]
BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] Ar
Cultivation of
scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		10

Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		11

PCR of
[J23110+B0034] Ar
Electrophoresis of
[J23110+B0034] Ar
12

Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
[J23110+B0034] Ar
Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		13

Plasmid purification of
[J23110+B0034] Ar
Transform of
[R0010]
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		14

Digestion of
[J23110+B0034] Ar (EcoRI and PstI)
GBP[FadL-GBP] Kr (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and SpeI)

Electrophoresis of
[J23110+B0034] Ar (EcoRI and PstI)
GBP[FadL-GBP] Kr (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and SpeI)
Transform of
[R0010] Cr
Cultivation of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		15

Digestion of
[pSB6A1]
[pSB3C5]
Anti-Her 2[Herceptin+E1010]
[J23101+B0034+OmpA+scFv-C]
[J23101+B0034+OmpA+scFv-B]
Cultivation of
[pSB6A1]
[R0010]
PCR of
OmpA[OmpA-linker] (EcoRI and PstI)
Transform of
[J23110+B0034] Kr
BFP[K592100+J61048] Kr 		16

Gel extraction of
OmpA[OmpA-linker] (EcoRI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Digestion of
OmpA[OmpA-linker] (EcoRI and PstI)
Transform of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr 		17

Electrophoresis of
OmpA[OmpA-linker] (EcoRI and PstI)
Anti-Her 2[Herceptin+E1010] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		18

Ligation of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
19

Transform of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		20

 21

 22

 23

 24

Transform of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
PCR of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
Electrophoresis of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		25

Cultivation of
[R0010] Cr
BFP[K592100+J61048] Kr
PCR of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
Electrophoresis of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
26

Plasmid purification of
[R0010] Cr
BFP[K592100+J61048] Kr
Digestion of
[R0010] Cr (EcoRI and SpeI)
BFP[K592100+J61048] Kr (EcoRI and PstI)
Electrophoresis of
[R0010] Cr
BFP[K592100+J61048] Kr
Ligation of
[R0010+B0034+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
BFP[B0030+K592100+J61048+pSB1C3] Cr
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
Ligation of
OmpA[OmpA-linker+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
Cultivation of
[pSB1C3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar 		27

PCR of
GBP[FadL-GBP]
Anti-Her 2[Herceptin+E1010]
Gel extraction of
GBP[FadL-GBP]
Anti-Her 2[Herceptin+E1010]
Transform of
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3]
BFP[B0030+K592100+J61048+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr
Gel extraction of
GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr
SDS-page of
[pSB1C3]
GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar
[pSB6A1] 		28

Ligation of
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr
BFP[B0030+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
PCR of
BFP[B0030+K592100+J61048+pSB1C3] Cr
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr
OmpA[J23110+B0034+OmpA+pSB3C5] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
SDS-page of
[pSB1C3]
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
Transform of
OmpA[OmpA-linker] Cr 		29

Electrophoresis of
OmpA[J23101+B0034+OmpA+pSB3C5]
Transform of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
PCR of
BFP[B0034+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Electrophoresis of
BFP[B0034+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Digestion of
Anti-Her 2[Herceptin-own] (EcoRI and PstI)
Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
GBP[GBP] (EcoRI and PstI)
Ligation of
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar
GFP[R0010+B0030+E0040+J61048] Ar
Electrophoresis of
Anti-Her 2[Herceptin-own] (EcoRI and PstI)
Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
GBP[GBP] (EcoRI and PstI)
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar
GFP[R0010+B0030+E0040+J61048] Ar 		30

Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Ligation of
GBP[FadL-GBP+pSB1C3]Cr
Digestion of
Anti-Her 2[Herceptin own] (NcoI and PstI)
[pSB1A3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
[J23101+B0034] Kr
Digestion of
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
Electrophoresis of
Anti-Her 2[Herceptin own] (NcoI and PstI)
[pSB1A3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
[J231-1+B0034] Kr
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI)
[J231-1+B0034] Kr (EcoRI and SpeI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI) 		31

Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI)
[J231-1+B0034] Kr (EcoRI and SpeI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Ligation of
Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar
Transform of
Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar
Electrophoresis of
[pSB1C3] (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Digestion of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Electrophoresis of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
2

Electrophoresis of
[pSB1C3] (XbaI and PstI)
OmpA[OmpA] (EcoRI and PstI)
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
OmpA[OmpA] (NcoI and PstI)
Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI) 		3

Transform of [R0010+B0034+pSB1K3] Kr
GBP[FadL-GBP+pSB1C3] Cr
BFP[B0030+K592100+J61048] Cr
Digestion of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		4

Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI)
PCR of
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		5

Digestion of
[pSB6A1] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Cultivation of
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
GBP[FadL-GBP] Cr
BFP[OmpA+K592100] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+oSB1C3] Cr
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr (EcoRI and PstI)
Transform of
GBP[J23101+B0034+FadL-GBP]Cr
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr
Gel extraction of
[pSB6A1] (EcoRI and PstI)
[OmpA] (NcoI and PstI)
[pSB1C3] (EcoRI and PstI) 		6

Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Ligation of
Anti-Her 2[OmpA+Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
Transform of
Anti-Her 2[OmpA+Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
[pSB1C3]
[pSB1A3]
[pSB1K3]
Plasmid purification of
BFP[B0030+K592100+J61048]
Digestion of
BFP[B0030+K592100+J61048] (EcoRI and PstI)
Cultivation of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Ligation of
[R0010+B0034] Kr
GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] Cr
Digestion of
[pSB1C3] (XbaI and PstI)
Electrophoresis of
GBP[J23101+B0034+FadL-GBP] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr
scFv-C[scFv-C] Cr
scFv-B[scFv-B] Cr
Plasmid purification of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Digestion of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
[B0034]
[pSB1C3]
Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Transform of
GBP[J23101+B0034+FadL-GBP] Ar
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr
[pSB1C3] (XbaI and PstI)
[pSB6A1] (XbaI and PstI)
[pSB1C3] (XbaI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr
GFP[J23101+B0034+E0040+J61048] Ar
SDS-page of
GFP[J23101+B0034+E0040+J61048] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Ar
[pSB6A1]
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Ar
Digestion of
[pSB1C3] (XbaI and PstI)
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Ligation of
[pSB1C3] (XbaI and PstI)
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[Herceptin-Own] Cr
BFP[J23101+B0034+OmpA+K592100] Cr 		7

Plasmid purification of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
PCR of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Plasmid purification of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
OmpA[OmpA] (NcoI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
Anti-Her 2[OmpA+Herceptin-Web] C
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Transform of
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
Anti-Her 2[OmpA+Herceptin-Web] C
BFP[J23101+B0034+OmpA+K592100] Cr 		8

Plasmid purification of
scFv-C[J23101+OmpA+scFv-C] Cr
scFv-B[J23101+OmpA+scFv-B] Cr
Digestion of
scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI)
scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Cultivation of
[pSB1K3]
[pSB1C3]
[pSB1A3]
PCR of
BFP[J23101+B0034+OmpA+K592100] Cr
Transform of
scFv-C[OmpA+scFv-C] Cr
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100] Cr
scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI)
scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Digestion of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and SpeI)
OmpA[OmpA] Cr NcoI and PstI
OmpA[OmpA+K592100] Cr NcoI and PstI
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr
Digestion of
Anti-Her 2[Herceptin-Own] (EcoRI and PstI)
Anti-Her 2[Herceptin-Web] (EcoRI and PstI)
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100] Cr
9

Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Plasmid purification of
[pSB1C3]
[pSB1K3]
[pSB1A3]
Digestion of
[pSB1C3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
Transform of
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr
[R0010+B0034] Cr
BFP[J23101+B0034+OmpA+K592100] Cr
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr (EcoRI and SpeI)
OmpA[OmpA] Cr (NcoI and PstI)
Digestion of
[pSB1A3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
Electrophoresis of
scFv-C[OmpA+scFv-C] Kr
PCR of
GBP[GBp+pSB1C3] Cr
Digestion of
BFP[B0030+K592100+J61048] Cr (XbaI and PstI) 		10

Electrophoresis of
GBP[GBp+pSB1C3] Cr
[pSB1A3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] Kr
Cultivation of
[R0010+B0034] Cr
BFP[B0030+K592100+J61048] Cr
scFv-C[OmpA+scFv-C] Kr
Digestion of
[pSB1C3] (EcoRI and Pstl)
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Gel extraction of
[pSB1C3] (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web]
Anti-Her 2[Herceptin-Web]
Anti-Her 2[Herceptin-Own] 		11

Plasmid purification of
[R0010+B0034] Cr
scFv-C[OmpA+scFv-C] Kr
BFP[OmpA+K592100] Cr

Gel extraction of
[pSB1C3] (EcoRI and PstI)
Digestion of
[R0010+B0034] Cr (EcoRI and SpeI)
scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI)
BFP[OmpA+K592100] Cr (EcoRI and PstI)
GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI)
Ligation of
Anti-Her 2[Herceptin-Web] Cr
PCR of
scFv-B[OmpA+scFv-B] Kr
Electrophoresis of
[R0010+B0034] Cr (EcoRI and SpeI)
scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI)
BFP[OmpA+K592100] Cr (EcoRI and PstI)
GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI)
Ligation of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Electrophoresis of
scFv-B[OmpA+scFv-B] Kr
PCR of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Transform of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr 		12

Electrophoresis of
scFv-B[OmpA+scFv-B] Kr
Transform of
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
PCR of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
[pSB1C3] (EcoRI and PstI)
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		13

Transform of
GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Cultivation of
[pSB1C3]
scFv-B[OmpA-scFv-B] Kr
Plasmid purification of
[pSB1C3]
scFv-B[OmpA-scFv-B] Kr
Digestion of
[pSB1C3] (EcoRI and PstI)
scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI)
Electrophoresis of
[pSB1C3] (EcoRI and PstI)
Ligation of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Electrophoresis of
[pSB1C3] (EcoRI and PstI)
scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI) 		14

Transform of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		15

PCR of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
PCR of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Electrophoresis of
GBP[FadL-GBP] (EcoRI and PstI)
16

PCR of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr
Cultivation of
OmpA[R0010+B0034+OmpA]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr 		17

Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		18

Plasmid purification of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI)
Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI)
BFP[B0030+K592100+J61048] Cr (XbaI and PstI)
OmpA[R0010+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI)
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] Cr
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
Ligation of
scFv-B[R0010+B0034+OmpA+scFv-B]
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
Anti-Her 2[Herceptin-Web]
scFv-B[J23101+B0034+OmpA+scFv-B]
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Anti-Her 2[Herceptin-Own]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		19

Electrophoresis of
Anti-Her 2[Herceptin-Web]
scFv-B[J23101+B0034+OmpA+scFv-B]
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Anti-Her 2[Herceptin-Own]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[OmpA+scFv-C]
Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI)
BFP[B0030+K592100+J61048] Cr (XbaI and PstI)
Transform of
GBP[R0010+B034+FadL-GBP] Ar
GBP[J23101+B0034+FadL-GBP] Ar
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr
PCR of
scFv-B[J23110+B0034+OmpA+scFv-B] 		20

PCR of
GBP[FadL-GBP] Kr
OmpA[OmpA] Cr
Gel extraction of
OmpA[OmpA] (EcoRI and SpeI)
Plasmid purification of
Anti-Her 2[Herceptin-Own] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
PCR of
GBP[FadL-GBP] Kr
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048]
scFv-B[R0010+B0034+OmpA+scFv-B]
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
Anti-Her 2[Herceptin-Own] Cr
Electrophoresis of
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048]
Ligation of
scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] Kr
PCR of
GBP[R0010+B0034+FadL-GBP]
GBP[J23110+B0034+FadL-GBP+B0030+E0040] 		21

Electrophoresis of
[pSB1K3] (EcoRI and PstI)
[R0010] Cr (EcoRI and SpeI)
[J23110] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] Ar (EcoRI and SpeI)
BFP[B0034+K592100+J61048] Cr (XbaI and PstI)
RFP[B0034+E1010+J61048] Cr (XbaI and PstI)
GBP[B0034+FadL-GBP+B0030+J61048] Cr (XbaI and PstI)
Ligation of
BFP[J23110+B0034+K592100+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+K592100+E1010+J61048pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3]
Transform of
scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] Kr
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
GBP[J23101+B0034+FadL-GBP]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040] 		22

Digestion of
[pSB1C3] (XbaI and SpeI)
PCR of
scFv-B[R0010+B0034+OmpA+scFv-B] Kr
scFv-C[R0010+B0034+OmpA+scFv-C] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-web] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] Ar
GBP[FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
BFP[J23110+B0034+K592100+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
Cultivation of
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[FadL-GBP] (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Digestion of
[pSB1C3] (XbaI and PstI)
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3]
GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Ar
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048]
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E0040+J61048]
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+K592100+J61048]
scFv-C[R0010+B0034+OmpA+scFv-C]
Cultivation of
GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Ar
23

Transform of
GBP[FadL-GBP] Ar
OmpA[OmpA] Cr
Plasmid purification of
Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] Kr
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] Kr
BFP[J23101+B0034+K592100+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP]
Anti-Her 2[R0010+B0034+Herceptin-own]
Transform of
Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] Kr
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] Kr
BFP[J23101+B0034+K592100+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Cultivation of
GBP[R0010+B0034+FadL-GBP]
GBP[J23110+B0034+fadL-GBP+B0030+E0040]
scFv-B[R0010+B0034+OmpA+scFv-B] Kr
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and SpeI)
GBP[B0034+FadL-GBP+B0030+E0040+J61048] (XbaI and PstI)
RFP[B0030+E1010+J61048] (XbaI and PstI)
BFP[J23101+B0034+K592100+J61048]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+Herceptin-web] (EcoRI and PstI)
Anti-Her 2[R0010+B0034+Herceptin-own] (EcoRI and PstI)
GBP[R0010+B0034+FadL-GBP+B0030+E0040] (EcoRI and PstI)
GBP[R0010+B0034FadL-GBP] (EcoRI and PstI)
[pSB1C3] (XbaI and PstI) 		24

Ligation of
scFv-B[OmpA+scFv-B] Kr
scFv-C[OmpA+scFv-C] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
Anti-Her 2[OmpA+Herceptin-web] Kr
Digestion of
Anti-Her 2[Herceptin-own] (NcoI and PstI)
Electrophoresis of
GBP[FadL-GBP] Cr (EcoRI and PstI)
GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
GBP[FadL-GBP] (EcoRI and PstI)
Plasmid purification of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP] Ar (EcoRI and SpeI)
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cr (EcoRI and PstI)
Transform of
GBP[FadL-GBP] Cr
OmpA[OmpA] Cr
PCR of
scFv-B[J23110+B0034+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+E0040+J61048+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+E1010+J61048+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] Kr
scFv-B[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
Transform of
scFv-B[OmpA+scFv-B] Kr
scFv-C[OmpA+scFv-C] Kr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
Anti-Her 2[OmpA+Herceptin-web] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
SDS-page of
scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
GFP[J23110+B0034+E0040+J61048
GBP[R0010+B0034+FadL-GBP]
[pSB1C3]
OmpA[R0010+B0034+OmpA+J61048]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
GBP[FadL-GBP] Cr
OmpA[OmpA]
Ligation of
BFP[J23101+B0034+OmpA+K592100] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+] Cr 		25

Digestion of
[pSB3C5] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+J61048] Cr (EcoRI and PstI)
scFv-B[R0010+B0034+OmpA+scFv-B] Kr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B030+E0040+J61048] Ar (EcoRI and PstI)
Cultivation of
GFP[J23101+B0034+E0040+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
PCR of
scFv-B[OmpA+scFv-B]
Anti-Her 2[OmpA+Herceptin-own]
GBP[FadL-GBP] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23101+B0034+OmpA+scFv-B +B0030+E0040+J61048]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Ligation of
scFv-C[OmpA+scFv-C] Kr
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] Kr
Plasmid purification of
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048]
Digestion of
scFv-C[R0010+B0034+OmpA+scFv-C] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
BFP[R0010+B0034+OmpA+K592100] (NcoI and PstI)
Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] Ar
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
OmpA[R0010+B0034+OmpA]
Digestion of
BFP[OmpA+K592100] (EcoRI and NcoI) 		26

Cultivation of
[pSB1C3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
Ligation of
GBP[FadL-GBP+pSB1C3] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
Electrophoresis of
[pSB1K3] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
scFv-C[R0010+B0034+OmpA+scFv-C]
Digestion of
GBP[FadL-GBP] (XbaI and PstI)
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+K592100]
Digestion of
[J23101] (EcoRI and SpeI)
BFP[OmpA+K592100] (EcoRI and NcoI)
Plasmid purification of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
BFP[R0010+B0034+OmpA+K592100]
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
[pSB1C3]
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] (EcoRI and PstI)
BFP[R0010+B0034+OmpA+K592100] (EcoRI and PstI)
scFv-B[OmpA+scFv-B] Kr(EcoRI and PstI I)
Anti-Her 2[OmpA+Herceptin-own] Kr (EcoRI and PstI)
[pSB1C3] 		27

Transform of
GBP[FadL-LGBP] Cr
Plasmid purification of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] Cr
scFv-C[OmpA+scFv-C] Kr
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
scFv-B [R0010+B0034+OmpA+scFv-B]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own]
Digestion of
GFP[B0034+E0040+J61048] (XbaI and PstI)
GBP[R0010+B0034+FadL-GBP] Ar (EcoRI and SpeI)
GFP[J23110+B0034+E0040+J61048] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
GFP[J23110+B0034+E0040+pSB6A1] 		28

PCR of
GBP[FadL-GBP] Cr
PCR of
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E1010+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048]
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+K592100+J61048]
Anti-Her 2[OmpA+Herceptin-own]
scFv-C[J23110+B0034+OmpA+scFv-C]
Anti-Her 2[J23110+B0034+Herceptin-own]
scFv-C[OmpA+scFv-C].
Transform of
Anti-Her 2[J23110+B0034+Herceptin-own]
BFP[B0030+K592100+J61084]
BFP[B0030+K592100+J61084]
BFP[J23101+B0034+OmpA+K592100]
Plasmid purification of
scFv-B[OmpA+scFv-B] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
Cultivation of
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E0040+J61048]
Anti-Her 2[J23110+B0034+Herceptin-own+B0030] 		29
30

Plasmid purification of
GBP[FadL-GBP]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] 		31


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