Difference between revisions of "Team:NCTU Formosa/Notebook"

 
(23 intermediate revisions by 3 users not shown)
Line 9: Line 9:
 
a, abbr, acronym, address, big, cite, code,
 
a, abbr, acronym, address, big, cite, code,
 
del, dfn, em, font, img, ins, kbd, q, s, samp,
 
del, dfn, em, font, img, ins, kbd, q, s, samp,
−
small, strike, strong, sub, sup, tt, var,
+
small, strike, strong, sub, tt, var,
 
b, u, i, center,
 
b, u, i, center,
 
dl, dt, dd, ol, ul, li,
 
dl, dt, dd, ol, ul, li,
Line 56: Line 56:
  
 
.p01{
 
.p01{
−
background-color:rgb(26,58,106);
+
background-color: #33CCFF;
 
position:relative;
 
position:relative;
 
height:70vh;
 
height:70vh;
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}
 
}
 
.background1{
 
.background1{
−
background-image:url("https://static.igem.org/mediawiki/2015/0/0e/NCTU_Formosa_backgorund_test.png");
+
background-image:url("https://static.igem.org/mediawiki/2015/d/d0/NCTU_Formosa_E.cotector7.png");
 
background-repeat:no-repeat;
 
background-repeat:no-repeat;
−
background-position:center;
+
background-position:center 13vh;
 
position:relative;
 
position:relative;
 
height:70vh;
 
height:70vh;
 
width:40vw;
 
width:40vw;
−
background-size:90%;
+
background-size:60%;
 
top:0vh;
 
top:0vh;
 
left:10vw;
 
left:10vw;
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}
 
}
 
.title{
 
.title{
−
font-family:Yu Gothic UI;
+
font-family:Arial;
 
font-size:33pt;
 
font-size:33pt;
 
position:relative;
 
position:relative;
 
right:20vw;
 
right:20vw;
−
top:20vh;
+
top:33vh;
 
color:#fff;
 
color:#fff;
 
z-index:100;
 
z-index:100;
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text-align:center;
 
text-align:center;
 
}
 
}
−
 
 
.content{
 
.content{
−
font-family:Calibri;
+
font-family:Arial;
 
font-size:20pt;
 
font-size:20pt;
 
width:60vw;
 
width:60vw;
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-webkit-border-top-right-radius:12px;
 
-webkit-border-top-right-radius:12px;
 
border-top-right-radius:12px;
 
border-top-right-radius:12px;
−
+
 
 
-moz-border-radius-topleft:12px;
 
-moz-border-radius-topleft:12px;
 
-webkit-border-top-left-radius:12px;
 
-webkit-border-top-left-radius:12px;
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vertical-align:middle;
 
vertical-align:middle;
 
 
−
+
overflow:auto;
 
border:1px solid #FFFFFF;
 
border:1px solid #FFFFFF;
 
border-width:0px 1px 1px 0px;
 
border-width:0px 1px 1px 0px;
 
text-align:left;
 
text-align:left;
−
padding:7px;
+
padding:0px;
−
font-size:14px;
+
font-size:10px;
 
font-family:Arial;
 
font-family:Arial;
 
font-weight:normal;
 
font-weight:normal;
 
color:#666666;
 
color:#666666;
 +
vertical-align:text-top;
 
}.CSSTableGenerator tr:last-child td{
 
}.CSSTableGenerator tr:last-child td{
 
border-width:0px 1px 0px 0px;
 
border-width:0px 1px 0px 0px;
 
}.CSSTableGenerator tr td:last-child{
 
}.CSSTableGenerator tr td:last-child{
 
border-width:0px 0px 1px 0px;
 
border-width:0px 0px 1px 0px;
−
        font-family:Arial;
 
−
        font-size:1;
 
 
}.CSSTableGenerator tr:last-child td:last-child{
 
}.CSSTableGenerator tr:last-child td:last-child{
 
border-width:0px 0px 0px 0px;
 
border-width:0px 0px 0px 0px;
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border-width:0px 0px 1px 1px;
 
border-width:0px 0px 1px 1px;
 
}
 
}
 +
.goto{
 +
position:relative;
 +
padding-left:20vw;
 +
padding-top:3vh;
 +
float:left;
 +
z-index:100;
 +
}
 +
.goto a{
 +
text-decoration:none;
 +
color:#000;
 +
}
 +
.goto1{
 +
position:relative;
 +
padding-left:76vw;
 +
padding-top:3vh;
 +
 +
}
 +
.goto1 a{
 +
text-decoration:none;
 +
color:#000;
 +
}
  
  
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<div class="abgne_tab">
 
<div class="abgne_tab">
 
<ul class="tabs">
 
<ul class="tabs">
−
<li><a href="#tab1">2015 March</a></li>
 
 
<li><a href="#tab2">2015 April</a></li>
 
<li><a href="#tab2">2015 April</a></li>
 
<li><a href="#tab3">2015 May</a></li>
 
<li><a href="#tab3">2015 May</a></li>
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<li><a href="#tab5">2015 July</a></li>
 
<li><a href="#tab5">2015 July</a></li>
 
<li><a href="#tab6">2015 August</a></li>
 
<li><a href="#tab6">2015 August</a></li>
−
<li><a href="#tab7">2015 September</a></li>
 
 
</ul>
 
</ul>
 
   
 
   
 
<div class="tab_container">
 
<div class="tab_container">
−
<div id="tab1" class="tab_content">
 
−
 
−
<div class="CSSTableGenerator" >
 
−
                <table >
 
−
                    <tr>
 
−
                        <td>
 
−
                          Sun.
 
−
                        </td>
 
−
                        <td >
 
−
                            Mon.
 
−
                        </td>
 
−
                        <td>
 
−
                            Tue.
 
−
                        </td>
 
−
<td>
 
−
                          Wed.
 
−
                        </td>
 
−
                        <td >
 
−
                            Thu.
 
−
                        </td>
 
−
                        <td>
 
−
                            Fri.
 
−
                        </td>
 
−
<td>
 
−
                            Set.
 
−
                        </td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                        <td >
 
−
                            <B>1</B><br><br>
 
−
PCR<br>
 
−
transform
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>2</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>3</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                          <B>4</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>5</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>6</B><br><br>
 
−
                        </td>
 
−
<td>
 
−
                            <B>7</B><br><br>
 
−
                        </td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                        <td >
 
−
                          <B>8</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>8</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>10</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                          <B>11</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>12</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>13</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                            <B>14</B><br><br>
 
−
                        </td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                        <td >
 
−
                            <B>15</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>16</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>17</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                            <B>18</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>19</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>20</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                          <B>21</B><br><br>
 
−
                        </td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                        <td >
 
−
                            <B>22</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>23</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>24</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                            <B>25</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>26</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>27</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                            <B>28</B><br><br>
 
−
                        </td>
 
−
                    </tr>
 
−
<tr>
 
−
                        <td >
 
−
                            <B>29</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                          <B>30</B><br><br>
 
−
                        </td>
 
−
                        <td>
 
−
                            <B>31</B><br><br>
 
−
                        </td>
 
−
<td >
 
−
                           
 
−
                        </td>
 
−
                        <td>
 
−
                           
 
−
                        </td>
 
−
                        <td>
 
−
                           
 
−
                        </td>
 
−
<td >
 
−
                           
 
−
                        </td>
 
−
                    </tr>
 
−
                </table>
 
−
            </div>
 
−
           
 
−
</div>
 
 
<div id="tab2" class="tab_content">
 
<div id="tab2" class="tab_content">
 
<div class="CSSTableGenerator" >
 
<div class="CSSTableGenerator" >
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<td >
 
<td >
 
                             <B>15</B><br><br>
 
                             <B>15</B><br><br>
−
Transformation of <br>GBP[FadL-GBP]<br>
+
<b>Transformation of</b><br>GBP[FadL-GBP]<br>
 
Cultivation of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 
Cultivation of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 
                         </td>
 
                         </td>
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Cultivation of <br>GBP[FadL-GBP]
 
Cultivation of <br>GBP[FadL-GBP]
 
<br>
 
<br>
−
Plasmid purification of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
+
<b>Plasmid purification of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 
<br>
 
<br>
−
Digestion of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+
<b>Digestion of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
  
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>17</B><br><br>Plasmid purification of
+
                             <B>17</B><br><br><b>Plasmid purification of</b>
 
<br>GBP[FadL-GBP]  
 
<br>GBP[FadL-GBP]  
 
<br>
 
<br>
−
Digestion of
+
<b>Digestion of</b>
 
<br>GBP[FadL-GBP]  (EcoRI and PstI)
 
<br>GBP[FadL-GBP]  (EcoRI and PstI)
 
<br>
 
<br>
−
Electrophoresis of digested product:
+
<b>Electrophoresis of</b><br>digested product:
 
<br>GBP[FadL-GBP]  (EcoRI and PstI)
 
<br>GBP[FadL-GBP]  (EcoRI and PstI)
  
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<td >
 
<td >
 
                           <B>18</B><br><br>
 
                           <B>18</B><br><br>
−
  Transformation of ligated product:  
+
  <b>Transformation of</b><br>ligated product:  
 
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
  
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                         <td >
 
                         <td >
 
                             <B>19</B><br><br>
 
                             <B>19</B><br><br>
−
Cultivation of  
+
<b>Cultivation of</b>
 
<br>ligated product: GBP[FadL-GBP] C<sup>r</sup>  
 
<br>ligated product: GBP[FadL-GBP] C<sup>r</sup>  
 
<br>PCR product: Terminator[J61048]
 
<br>PCR product: Terminator[J61048]
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                         <td>
 
                         <td>
 
                             <B>21</B><br><br>
 
                             <B>21</B><br><br>
−
Cultivation of
+
<b>Cultivation of</b>
 
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
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<td >
 
<td >
 
                             <B>22</B><br><br>
 
                             <B>22</B><br><br>
−
Plasmid purification of  
+
<b>Plasmid purification of</b>
 
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 
<br>GBP[FadL-GBP+PSB1C3] C<sup>r</sup>
 
<br>GBP[FadL-GBP+PSB1C3] C<sup>r</sup>
 
<br>
 
<br>
−
Digestion of  
+
<b>Digestion of</b>
 
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 
<br>
 
<br>
−
Electrophoresis of digested product:  
+
<b>Electrophoresis of</b><br>digested product:  
 
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
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                         <td>
 
                         <td>
 
                             <B>23</B><br><br>
 
                             <B>23</B><br><br>
−
Digestion of
+
<b>Digestion of</b>
 
OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 
OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 
<br>
 
<br>
−
Electrophoresis of digested product:
+
<b>Electrophoresis of</b><br>digested product:
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
Line 621: Line 490:
 
                         <td>
 
                         <td>
 
                             <B>24</B><br><br>
 
                             <B>24</B><br><br>
−
Digestion of
+
<b>Digestion of</b>
 
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
Line 629: Line 498:
 
<td >
 
<td >
 
                             <B>25</B><br><br>
 
                             <B>25</B><br><br>
−
Electrophoresis of digested product:
+
<b>Electrophoresis of</b><br>digested product:
 
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 
<br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 
<br>
 
<br>
−
Digestion of
+
<b>Digestion of</b>
 
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>
 
<br>
−
Electrophoresis of digested product:
+
<b>Electrophoresis of</b><br>digested product:
 
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 
<br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 
<br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>
 
<br>
−
Ligation of
+
<b>Ligation of</b>
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>GBP[GBP+B0034+pSB1K3] K<sup>r</sup>
 
<br>GBP[GBP+B0034+pSB1K3] K<sup>r</sup>
Line 653: Line 522:
 
                         <td >
 
                         <td >
 
                             <B>26</B><br><br>
 
                             <B>26</B><br><br>
−
Ligation of
+
<b>Ligation of</b>
 
<br>RFP[E1010+B0034+pSB1K3] K<sup>r</sup>  
 
<br>RFP[E1010+B0034+pSB1K3] K<sup>r</sup>  
 
<br>
 
<br>
−
Transformation of ligated product:
+
<b>Transformation</b><br>of ligated product:
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>GBP[FadL-GBP+B0034+pSB1K3] K<sup>r</sup>
 
<br>GBP[FadL-GBP+B0034+pSB1K3] K<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 
<br>
 
<br>
−
Electrophoresis of digested product:
+
<b>Electrophoresis of</b><br>digested product:
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
 
<br>[J23101+B0034]  
 
<br>[J23101+B0034]  
Line 668: Line 537:
 
                         <td>
 
                         <td>
 
                           <B>27</B><br><br>
 
                           <B>27</B><br><br>
−
  Digestion of
+
  <b>Digestion of</b>
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 
<br>
 
<br>
−
PCR of ligated product:
+
<b>PCR of</b><br>ligated product:
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
Line 678: Line 547:
 
                         <td>
 
                         <td>
 
                             <B>28</B><br><br>
 
                             <B>28</B><br><br>
−
Electrophoresis of
+
<b>Electrophoresis of</b>
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
  
−
Transformation of ligated product:
+
<b>Transformation of</b><br>ligated product:
 
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 
<br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
  
−
PCR of ligated product
+
<b>PCR of</b><br>ligated product
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
  
Line 692: Line 561:
 
<td >
 
<td >
 
                             <B>29</B><br><br>
 
                             <B>29</B><br><br>
−
Cultivation of
+
<b>Cultivation of</b>
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>  
  
−
PCR of
+
<b>PCR of</b>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
  
−
<br>Electrophoresis of
+
<br><b>Electrophoresis of</b>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
  
  
−
Ligation of
+
<b>Ligation of</b>
 
<br>GBP[FadL-GBP+J61048+pSB1K3] K<sup>r</sup>
 
<br>GBP[FadL-GBP+J61048+pSB1K3] K<sup>r</sup>
  
Line 708: Line 577:
 
                         <td>
 
                         <td>
 
                             <B>30</B><br><br>
 
                             <B>30</B><br><br>
−
  Plasmid purification of
+
  <b>Plasmid purification of</b>
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
  
−
Ligation of
+
<b>Ligation of</b>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
  
−
PCR of
+
<b>PCR of</b>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
  
−
Transformation of  
+
<b>Transformation of</b>
 
<br>mini: Terminator[J61048]
 
<br>mini: Terminator[J61048]
 
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
Line 775: Line 644:
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>1</B><br><br>
+
                             <B>1</B><br><br><b>PCR of</b><br>Transformation product:
 +
<br>mini: Terminator[J61048]
 +
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>mini: Terminator[J61048]
 +
<br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 
 +
 
 
                         </td>
 
                         </td>
 
<td>
 
<td>
Line 783: Line 661:
 
                     <tr>
 
                     <tr>
 
                         <td >
 
                         <td >
−
                           <B>3</B><br><br>
+
                           <B>3</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>4</B><br><br>
+
                           <B>4</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
5/4
 +
<b>Transformation off</b> ligated products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
 +
<br>
 +
<b>PCR of</b> ligated products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>5</B><br><br>
+
                           <B>5</B><br><br><b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP+J61048] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b> PCR product:
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>6</B><br><br>
+
                           <B>6</B><br><br><b>Plasmid purification of</b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b> PCR product:
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b> backbone:
 +
<br>[pSB1C3] C<sup>r</sup>
 +
<br>[pSB1A3] A<sup>r</sup>
 +
<br>[pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>7</B><br><br>
+
                             <B>7</B><br><br><b>Digestion of </b>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>[pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>8</B><br><br>
+
                             <B>8</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transformation off</b> ligated products:
 +
<br>RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>9</B><br><br>
+
                             <B>9</B><br><br><b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
 
                         <td >
 
                         <td >
−
                             <B>10</B><br><br>
+
                             <B>10</B><br><br><b>Electrophoresis of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>
 +
<br><b>Plasmid purification of</b>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>[B0030] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[B0030] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>[B0030] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>11</B><br><br>
+
                             <B>11</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>12</B><br><br>
+
                             <B>12</B><br><br><b>PCR of</b> ligated products:
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b> PCR products:
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>PCR products: RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>13</B><br><br>
+
                             <B>13</B><br><br><b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>14</B><br><br>
+
                             <B>14</B><br><br><b>Cultivation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>15</B><br><br>
+
                             <B>15</B><br><br><b>Digestion of </b>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>16</B><br><br>
+
                           <B>16</B><br><br><b>Transformation off</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
 
                         <td >
 
                         <td >
−
                             <B>17</B><br><br>
+
                             <B>17</B><br><br><b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b> PCR products:
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>18</B><br><br>
+
                           <B>18</B><br><br><b>Cultivation of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GFP[B0030+E0040+J61048]
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>19</B><br><br>
+
                             <B>19</B><br><br><b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>digested products:
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>20</B><br><br>
+
                             <B>20</B><br><br><b>Cultivation of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and Pst1)
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<b>Transformation off</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>[pSB3C5] 抗性
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>21</B><br><br>
+
                             <B>21</B><br><br><b>Digestion of </b>
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>22</B><br><br>
+
                             <B>22</B><br><br><b>Cultivation of</b>
 +
<br>[pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>23</B><br><br>
+
                             <B>23</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transformation off</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
 
<tr>
 
<tr>
 
                         <td >
 
                         <td >
−
                             <B>24</B><br><br>
+
                             <B>24</B><br><br><b>Electrophoresis of</b> digested products:
 +
<br>[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23101+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>25</B><br><br>
+
                           <B>25</B><br><br><b>Transformation off</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[B003+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GFP[B0030+E0040+J61048]
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[B0030+E0040+J61048]  (XbaI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>26</B><br><br>
+
                             <B>26</B><br><br><b>Ligation of</b>
 +
<br>OmpA[J23110+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>27</B><br><br>
+
                             <B>27</B><br><br><b>PCR of</b>
 +
<br>[J23101+B0034+OmpA+pSB3C5]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
 +
<br>GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>28</B><br><br>
+
                             <B>28</B><br><br><b>Ligation of</b>
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性??
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>[J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S)
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<br>Plasmid purification in gel slices of PCR products:
 +
<br>OmpA[EX-OmpA-S]
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>OmpA[OmpA] (EcoRI and SpeI)
 +
<br>
 +
<b>Transformation off</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5]
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>29</B><br><br>
+
                             <B>29</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23110] A<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>30</B><br><br>
+
                             <B>30</B><br><br><b>PCR of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
 
<tr>
 
<tr>
 
                         <td >
 
                         <td >
−
                             <B>31</B><br><br>
+
                             <B>31</B><br><br><b>Cultivation of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
 +
<br>GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
Line 929: Line 1,263:
 
</td>
 
</td>
 
                         <td >
 
                         <td >
−
                             <B>1</B><br><br>
+
                             <B>1</B><br><br><b>Electrophoresis of</b>
 +
<br>[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>GFP[K1489002+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>[pSB3C5] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GFP[J23101+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
 +
<br>GFP[J23110+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
 +
<br>
 +
<b>Electrophoresis of</b> digested product:
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[OmpA+K592100]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>2</B><br><br>
+
                             <B>2</B><br><br><b>PCR of</b>
 +
<br>OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
 +
<br>OmpA[OmpA] (EcoRI and NcoI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1A3] (EcoRI and NcoI)
 +
<br>
 +
<b>Electrophoresis of</b> digested products:
 +
<br>[pSB1A3] (EcoRI and NcoI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>3</B><br><br>
+
                           <B>3</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA+psB1C3] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA+psB1C3] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+ K592100] (NcoI and PstI)
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>4</B><br><br>
+
                           <B>4</B><br><br><b>Cultivation of</b>
 +
<br>[J23110+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>5</B><br><br>
+
                           <B>5</B><br><br><b>Transform of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>[J23110+pSb1A3] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3]
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>6</B><br><br>
+
                             <B>6</B><br><br><b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
 
 
                         </td>
 
                         </td>
 
 
Line 950: Line 1,380:
 
                     <tr>
 
                     <tr>
 
<td>
 
<td>
−
                             <B>7</B><br><br>
+
                             <B>7</B><br><br><b>Gel extraction of</b>
 +
<br>OmpA[EX-OmpA-S] 抗性
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
−
                           <B>8</B><br><br>
+
                           <B>8</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3K3]
 +
[<br>pSB6A1]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[EX-OmpA-S] (EcoRI and SpeI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>8</B><br><br>
+
                           <B>9</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB3K3]
 +
<br>[pSB6A1]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3K3] (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>10</B><br><br>
+
                           <B>10</B><br><br><b>Transform of</b>
 +
<br>[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[J23101+B0034+OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3K3] (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3K3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>11</B><br><br>
+
                           <B>11</B><br><br><b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1K3] K<sup>r</sup>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>12</B><br><br>
+
                             <B>12</B><br><br><b>PCR of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>[B0034+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>13</B><br><br>
+
                             <B>13</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
</tr>
 
</tr>
Line 974: Line 1,517:
 
                    
 
                    
 
<tr><td >
 
<tr><td >
−
                             <B>14</B><br><br>
+
                             <B>14</B><br><br><b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB3C5]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B]
 +
<br>scFv-C[OmpA+scFv-C]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110]
 +
<br>[B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
Line 980: Line 1,554:
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>16</B><br><br>
+
                             <B>16</B><br><br><b>Transform of</b>
 +
<br>[pSB3C5]
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>17</B><br><br>
+
                             <B>17</B><br><br><b>Cultivation of</b>
 +
<br>[pSB3C5]
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>18</B><br><br>
+
                             <B>18</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB3C5]( EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB3C5]( EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>19</B><br><br>
+
                             <B>19</B><br><br><b>Transform of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>20</B><br><br>
+
                             <B>20</B><br><br><b>PCR of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
 
Line 1,003: Line 1,603:
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>23</B><br><br>
+
                           <B>23</B><br><br><b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB3C5]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>BFP[OmpA+K592100]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1]
 +
<br>[psB6A1]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>24</B><br><br>
+
                             <B>24</B><br><br><b>Plasmid purification of</b>
 +
<br>[pSB3C5]
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>25</B><br><br>
+
                             <B>25</B><br><br><b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
Line 1,015: Line 1,637:
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>27</B><br><br>
+
                             <B>27</B><br><br><b>Digestion of </b>
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] 抗性 (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
 
 
                     </tr>
 
                     </tr>
 
<tr><td >
 
<tr><td >
−
                             <B>28</B><br><br>
+
                             <B>28</B><br><br><b>Cultivation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>[pSB3C5]
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23101+B0034] K<sup>r</sup>
 +
<br>GBP[FadL-GBP] 抗性 (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[J23101+B0034+OmpA+K592100] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
<br>[E0040] A<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>[B0034+E0040+pSB1K3] K<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1A3]
 +
<br>[pSB1C3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3C5]
 +
<br>[pSB6A1]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
−
                             <B>29</B><br><br>
+
                             <B>29</B><br><br><b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>30</B><br><br>
+
                           <B>30</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
Line 1,079: Line 1,773:
 
</td>
 
</td>
 
                         <td >
 
                         <td >
−
                             <B>1</B><br><br>
+
                             <B>1</B><br><br><b>Cultivation of</b>
−
PCR<br>
+
<br>[pSB6A1]
−
transform
+
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[]FadL-GBP] 抗性 (EcoRI and PstI)
 +
 
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>2</B><br><br>
+
                             <B>2</B><br><br><b>Digestion of </b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>
 +
<br>Plasmid purification in gel slices of
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>3</B><br><br>
+
                           <B>3</B><br><br><b>Gel extraction of</b>
 +
<br>[J23110] A<sup>r</sup> (SpeI and PstI)
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] (EcoRI and Pstl)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>4</B><br><br>
+
                           <B>4</B><br><br><b>Transform of</b>
 +
<br>GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                          
 
                          
Line 1,096: Line 1,843:
 
                     </tr>
 
                     </tr>
 
                     <tr><td>
 
                     <tr><td>
−
                           <B>5</B><br><br>
+
                           <B>5</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td><td>
 
                         </td><td>
−
                             <B>6</B><br><br>
+
                             <B>6</B><br><br><b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td>
 
<td>
−
                             <B>7</B><br><br>
+
                             <B>7</B><br><br><b>Digestion of </b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[pSB1A3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
−
                           <B>8</B><br><br>
+
                           <B>8</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>9</B><br><br>
+
                           <B>9</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB3C5]
 +
<br>BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>10</B><br><br>
+
                           <B>10</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>11</B><br><br>
+
                           <B>11</B><br><br><b>PCR of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                          
 
                          
 
                     </tr>
 
                     </tr>
 
                     <tr><td>
 
                     <tr><td>
−
                             <B>12</B><br><br>
+
                             <B>12</B><br><br><b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>13</B><br><br>
+
                             <B>13</B><br><br><b>Plasmid purification of</b>
 +
<br>[J23110+B0034] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>14</B><br><br>
+
                             <B>14</B><br><br><b>Digestion of </b>
 +
<br>[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<br><b>Electrophoresis of</b>
 +
<br>[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
−
                             <B>15</B><br><br>
+
                             <B>15</B><br><br><b>Digestion of </b>
 +
<br>[pSB6A1]
 +
<br>[pSB3C5]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>[J23101+B0034+OmpA+scFv-C]
 +
<br>[J23101+B0034+OmpA+scFv-B]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB6A1]
 +
<br>[R0010]
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>[J23110+B0034] K<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>16</B><br><br>
+
                             <B>16</B><br><br><b>Gel extraction of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>17</B><br><br>
+
                             <B>17</B><br><br><b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin+E1010] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>18</B><br><br>
+
                             <B>18</B><br><br><b>Ligation of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                          
 
                          
Line 1,142: Line 2,019:
 
                     </tr>
 
                     </tr>
 
                     <tr><td>
 
                     <tr><td>
−
                             <B>19</B><br><br>
+
                             <B>19</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td><td>
 
                         </td><td>
 
                             <B>20</B><br><br>
 
                             <B>20</B><br><br>
Line 1,156: Line 2,036:
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>24</B><br><br>
+
                             <B>24</B><br><br><b>Transform of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>25</B><br><br>
+
                             <B>25</B><br><br><b>Cultivation of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup> 
 +
<br>Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                          
 
                          
Line 1,165: Line 2,067:
 
                     </tr>
 
                     </tr>
 
<tr><td>
 
<tr><td>
−
                             <B>26</B><br><br>
+
                             <B>26</B><br><br><b>Plasmid purification of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[R0010] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[K592100+J61048] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[R0010] C<sup>r</sup>
 +
<br>BFP[K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td><td>
 
                         </td><td>
−
                             <B>27</B><br><br>
+
                             <B>27</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[Herceptin+E1010]
 +
<br>
 +
<b>Transform of</b>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3]
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
 +
<br>[pSB6A1]
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>28</B><br><br>
+
                             <B>28</B><br><br><b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[R0010+B0034+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
 +
<br>OmpA[J23110+B0034+OmpA+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>[pSB1C3]
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
 +
<br>GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>OmpA[OmpA-linker] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td >
 
                         <td >
−
                             <B>29</B><br><br>
+
                             <B>29</B><br><br><b>Electrophoresis of</b>
 +
<br>OmpA[J23101+B0034+OmpA+pSB3C5]
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
 +
<br>GBP[GBP] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
 +
<br>GBP[GBP] (EcoRI and PstI)
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>30</B><br><br>
+
                           <B>30</B><br><br><b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB1C3]C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin own]  (NcoI and PstI)
 +
<br>[pSB1A3]  (EcoRI and PstI)
 +
<br>[pSB1C3]  (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>[J23101+B0034] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin own]  (NcoI and PstI)
 +
<br>[pSB1A3]  (EcoRI and PstI)
 +
<br>[pSB1C3]  (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>[J231-1+B0034] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>31</B><br><br>
+
                             <B>31</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
Line 1,232: Line 2,308:
 
<td >
 
<td >
 
                             <B>1</B><br><br>
 
                             <B>1</B><br><br>
−
PCR<br>
+
<b>Digestion of </b>
−
transform
+
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
 
 
                         </td></tr>
 
                         </td></tr>
 
                     <tr>
 
                     <tr>
 
                          
 
                          
 
                         <td>
 
                         <td>
−
                             <B>2</B><br><br>
+
                             <B>2</B><br><br><b>Electrophoresis of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>OmpA[OmpA] (EcoRI and PstI)
 +
<br>scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
 +
<br>scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>3</B><br><br>
+
                           <B>3</B><br><br><b>Transform of</b>
 +
[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>4</B><br><br>
+
                           <B>4</B><br><br><b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>[R0010+B0034+pSB1K3] K<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>5</B><br><br>
+
                           <B>5</B><br><br><b>Digestion of </b>
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[scFv-B+oSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP]C<sup>r</sup>
 +
<br>scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB6A1] (EcoRI and PstI)
 +
<br>[OmpA] (NcoI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>6</B><br><br>
+
                             <B>6</B><br><br><b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>[pSB1K3]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>BFP[B0030+K592100+J61048]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[B0030+K592100+J61048]  (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[R0010+B0034] K<sup>r</sup>
 +
<br>GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[scFv-C] C<sup>r</sup>
 +
<br>scFv-B[scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>[B0034]
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>[pSB6A1] (XbaI and PstI)
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>[pSB6A1]
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td>
 
<td>
−
                             <B>7</B><br><br>
+
                             <B>7</B><br><br><b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>OmpA[OmpA] (NcoI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-Web] C
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 
 
                         </td> <td >
 
                         </td> <td >
−
                           <B>8</B><br><br>
+
                           <B>8</B><br><br><b>Plasmid purification of</b>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup>
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1K3]
 +
<br>[pSB1C3]
 +
<br>[pSB1A3]
 +
<br>
 +
<b>PCR of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[OmpA+scFv-C] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA] C<sup>r</sup> NcoI and PstI
 +
<br>OmpA[OmpA+K592100] C<sup>r</sup> NcoI and PstI
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>Anti-Her 2[Herceptin-Own] (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Web] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
Line 1,261: Line 2,599:
 
                        
 
                        
 
                         <td>
 
                         <td>
−
                           <B>8</B><br><br>
+
                           <B>9</B><br><br><b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>[pSB1K3]
 +
<br>[pSB1A3]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (NcoI and PstI)
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[GBp+pSB1C3] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                           <B>10</B><br><br>
+
                           <B>10</B><br><br><b>Electrophoresis of</b>
 +
<br>GBP[GBp+pSB1C3] C<sup>r</sup>
 +
<br>[pSB1A3] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and Pstl)
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web]
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>Anti-Her 2[Herceptin-Own]
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                           <B>11</B><br><br>
+
                           <B>11</B><br><br><b>Plasmid purification of</b>
 +
<br>[R0010+B0034] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup>
 +
<br>
 +
<br><b>Gel extraction of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b><b><b>Digestion of </b> </b></b>
 +
<br>[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>12</B><br><br>
+
                             <B>12</B><br><br><b>Electrophoresis of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[Herceptin-Web] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>13</B><br><br>
+
                             <B>13</B><br><br><b>Transform of</b>
 +
<br>GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>[pSB1C3]
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>14</B><br><br>
+
                             <B>14</B><br><br><b>Transform of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td><td >
 
                         </td><td >
−
                             <B>15</B><br><br>
+
                             <B>15</B><br><br><b>PCR of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup> 
 +
<br>BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
Line 1,284: Line 2,795:
 
                          
 
                          
 
                         <td>
 
                         <td>
−
                             <B>16</B><br><br>
+
                             <B>16</B><br><br><b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>OmpA[R0010+B0034+OmpA]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>17</B><br><br>
+
                             <B>17</B><br><br><b>Ligation of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>18</B><br><br>
+
                             <B>18</B><br><br><b>Plasmid purification of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Digestion of </b>
 +
<br>BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>19</B><br><br>
+
                             <B>19</B><br><br><b>Electrophoresis of</b>
 +
<br>Anti-Her 2[Herceptin-Web]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
 +
<br>Anti-Her 2[Herceptin-Own]
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C]
 +
<br>Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
 +
<br>Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[R0010+B034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br><b>PCR of</b>
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B]
 +
 
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>20</B><br><br>
+
                             <B>20</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP] K<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>Gel extraction of</b>
 +
<br>OmpA[OmpA] (EcoRI and SpeI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] K<sup>r</sup>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup> 
 +
<br>GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048]
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
 +
<br>
 +
<b>Digestion of </b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
 +
<br>Anti-Her 2[Herceptin-Own] C<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup> 
 +
<br>GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] A<sup>r</sup> 
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br><b>PCR of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040]
 +
 
 +
 
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
 
                           <B>21</B><br><br>
 
                           <B>21</B><br><br>
 +
  <b>Electrophoresis of</b>
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>[R0010] C<sup>r</sup> (EcoRI and SpeI)
 +
<br>[J23110] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>BFP[B0034+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>RFP[B0034+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
 +
<br>GBP[B0034+FadL-GBP+B0030+J61048] C<sup>r</sup> (XbaI and PstI)
 +
 +
<br><b>Ligation of</b>
 +
<br>BFP[J23110+B0034+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+K592100+E1010+J61048pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3]
 +
 +
<br><b>Transform of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048pSB1A3] A<sup>r</sup> 
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
<br>GBP[J23101+B0034+FadL-GBP]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040]
 +
 
                         </td><td >
 
                         </td><td >
 
                             <B>22</B><br><br>
 
                             <B>22</B><br><br>
 +
<b>Digestion of</b>
 +
<br>[pSB1C3] (XbaI and SpeI)
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] A<sup>r</sup>
 +
<br>GBP[FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>BFP[J23110+B0034+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>[pSB6A1]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[FadL-GBP] (XbaI and PstI)
 +
<br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Digestion of</b>
 +
<br>[pSB1C3] (XbaI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3]
 +
<br>GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E0040+J61048]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+K592100+J61048]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
Line 1,308: Line 3,015:
 
                         <td>
 
                         <td>
 
                           <B>23</B><br><br>
 
                           <B>23</B><br><br>
 +
  <b>Transform of</b>
 +
<br>GBP[FadL-GBP] A<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>BFP[J23101+B0034+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own]
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>BFP[J23101+B0034+K592100+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>GBP[J23110+B0034+fadL-GBP+B0030+E0040]
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup>
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and SpeI)
 +
<br>GBP[B0034+FadL-GBP+B0030+E0040+J61048] (XbaI and PstI)
 +
<br>RFP[B0030+E1010+J61048] (XbaI and PstI)
 +
<br>BFP[J23101+B0034+K592100+J61048]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-web] (EcoRI and PstI)
 +
<br>Anti-Her 2[R0010+B0034+Herceptin-own] (EcoRI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] (EcoRI and PstI)
 +
<br>GBP[R0010+B0034FadL-GBP] (EcoRI and PstI)
 +
<br>[pSB1C3] (XbaI and PstI)
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>24</B><br><br>
 
                             <B>24</B><br><br>
 +
<b>Ligation of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b>
 +
<br>Anti-Her 2[Herceptin-own] (NcoI and PstI)
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
<br>GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
 +
<br>GBP[FadL-GBP] (EcoRI and PstI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>OmpA[OmpA] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[J23110+B0034+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+E0040+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+E1010+J61048+pSB1K3] K<sup>r</sup>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>scFv-B[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-web] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>
 +
<b>SDS-page of</b>
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
 +
<br>GFP[J23110+B0034+E0040+J61048
 +
<br>GBP[R0010+B0034+FadL-GBP]
 +
<br>[pSB1C3]
 +
<br>OmpA[R0010+B0034+OmpA+J61048]
 +
<br>GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>OmpA[OmpA]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+] C<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>25</B><br><br>
+
                             <B>25</B><br><br><b>Digestion of</b>
 +
<br>[pSB3C5] (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+J61048] C<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-B[R0010+B0034+OmpA+scFv-B] K<sup>r</sup> (EcoRI and PstI)
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>GFP[J23101+B0034+E0040+J61048] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>PCR of</b>
 +
<br>scFv-B[OmpA+scFv-B]
 +
<br>Anti-Her 2[OmpA+Herceptin-own]
 +
<br>GBP[FadL-GBP] A<sup>r</sup>
 +
<br>
 +
<b>Transform of</b>
 +
<br>scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048]
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B +B0030+E0040+J61048]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
 +
<br>scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] K<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]  (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] (EcoRI and PstI)
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>BFP[R0010+B0034+OmpA+K592100] (NcoI and PstI)
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] A<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>OmpA[R0010+B0034+OmpA]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>BFP[OmpA+K592100] (EcoRI and NcoI)
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>26</B><br><br>
 
                             <B>26</B><br><br>
 +
<b>Cultivation of</b>
 +
<br>[pSB1C3]
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040] K<sup>r</sup>
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
 +
<br>
 +
<b>Electrophoresis of</b>
 +
<br>[pSB1K3] (EcoRI and PstI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[FadL-GBP] (XbaI and PstI)
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+K592100]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>[J23101] (EcoRI and SpeI)
 +
<br>BFP[OmpA+K592100] (EcoRI and NcoI)
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>BFP[R0010+B0034+OmpA+K592100]
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>[pSB1C3]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] (EcoRI and PstI)
 +
<br>BFP[R0010+B0034+OmpA+K592100] (EcoRI and PstI)
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>(EcoRI and PstI I)
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup> (EcoRI and PstI)
 +
<br>[pSB1C3]
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>27</B><br><br>
+
                             <B>27</B><br><br><b>Transform of</b>
 +
<br>GBP[FadL-LGBP] C<sup>r</sup>
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
<br>
 +
<b>Ligation of</b>
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] C<sup>r</sup>
 +
<br>scFv-C[OmpA+scFv-C] K<sup>r</sup>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>Anti-Her 2[OmpA+Herceptin-own] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>scFv-B [R0010+B0034+OmpA+scFv-B]
 +
<br>Anti-Her 2[R0010+B0034+OmpA+Herceptin-own]
 +
<br>
 +
<b>Digestion of</b>
 +
<br>GFP[B0034+E0040+J61048] (XbaI and PstI)
 +
<br>GBP[R0010+B0034+FadL-GBP] A<sup>r</sup> (EcoRI and SpeI)
 +
<br>GFP[J23110+B0034+E0040+J61048] (EcoRI and PstI)
 +
<br>[pSB1C3] (EcoRI and PstI)
 +
<br>
 +
<b>Ligation of</b>
 +
<br>GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
 +
<br>GFP[J23110+B0034+E0040+pSB6A1]
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>28</B><br><br>
+
                             <B>28</B><br><br><b>PCR of</b>
 +
<br>GBP[FadL-GBP] C<sup>r</sup>
 +
<br>
 +
<b>PCR of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E1010+J61048]
 +
<br>scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+K592100+J61048]
 +
<br>Anti-Her 2[OmpA+Herceptin-own]
 +
<br>scFv-C[J23110+B0034+OmpA+scFv-C]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own]
 +
<br>scFv-C[OmpA+scFv-C].
 +
<br>
 +
<b>Transform of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own]
 +
<br>BFP[B0030+K592100+J61084]
 +
<br>BFP[B0030+K592100+J61084]
 +
<br>BFP[J23101+B0034+OmpA+K592100]
 +
<br>
 +
<b>Plasmid purification of</b>
 +
<br>scFv-B[OmpA+scFv-B] K<sup>r</sup>
 +
<br>scFv-C[R0010+B0034+OmpA+scFv-C]
 +
<br>
 +
<b>Cultivation of</b>
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E0040+J61048]
 +
<br>Anti-Her 2[J23110+B0034+Herceptin-own+B0030]
 +
 
 
                         </td><td >
 
                         </td><td >
 
                             <B>29</B><br><br>
 
                             <B>29</B><br><br>
Line 1,330: Line 3,279:
 
                          
 
                          
 
                         <td>
 
                         <td>
−
                           <B>30</B><br><br>
+
                           <B>30</B><br><br><b>Plasmid purification of</b>
 +
<br>GBP[FadL-GBP]
 +
<br>Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
 +
 
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
Line 1,353: Line 3,305:
 
             </div>
 
             </div>
 
</div>
 
</div>
−
<div id="tab7" class="tab_content">
+
−
<div class="CSSTableGenerator" >
+
−
                <table >
+
−
                    <tr>
+
−
                        <td>
+
−
                          Sun.
+
−
                        </td>
+
−
                        <td >
+
−
                            Mon.
+
−
                        </td>
+
−
                        <td>
+
−
                            Tue.
+
−
                        </td>
+
−
<td>
+
−
                          Wed.
+
−
                        </td>
+
−
                        <td >
+
−
                            Thu.
+
−
                        </td>
+
−
                        <td>
+
−
                            Fri.
+
−
                        </td>
+
−
<td>
+
−
                            Set.
+
−
                        </td>
+
−
                    </tr>
+
−
                    <tr>
+
−
<td>
+
−
</td>
+
−
<td>
+
−
</td>
+
−
                        <td >
+
−
                            <B>1</B><br><br>
+
−
PCR<br>
+
−
transform
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>2</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>3</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                          <B>4</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>5</B><br><br>
+
−
                        </td>
+
−
                       
+
−
                    </tr>
+
−
                    <tr><td>
+
−
                            <B>6</B><br><br>
+
−
                        </td>
+
−
<td>
+
−
                            <B>7</B><br><br>
+
−
                        </td>
+
−
                        <td >
+
−
                          <B>8</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>9</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>10</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                          <B>11</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>12</B><br><br>
+
−
                        </td>
+
−
                     
+
−
                    </tr>
+
−
                    <tr> <td>
+
−
                            <B>13</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                            <B>14</B><br><br>
+
−
                        </td>
+
−
                        <td >
+
−
                            <B>15</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>16</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>17</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                            <B>18</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>19</B><br><br>
+
−
                        </td>
+
−
                     
+
−
                    </tr>
+
−
                    <tr> <td>
+
−
                            <B>20</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                          <B>21</B><br><br>
+
−
                        </td>
+
−
                        <td >
+
−
                            <B>22</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>23</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>24</B><br><br>
+
−
                        </td>
+
−
<td >
+
−
                            <B>25</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                            <B>26</B><br><br>
+
−
                        </td>
+
−
                       
+
−
+
−
                    </tr>
+
−
<tr><td>
+
−
                            <B>27</B><br><br>
+
−
                        </td><td >
+
−
                            <B>28</B><br><br>
+
−
                        </td>
+
−
                        <td >
+
−
                            <B>29</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                          <B>30</B><br><br>
+
−
                        </td>
+
−
                        <td>
+
−
                         
+
−
                        </td>
+
−
<td >
+
−
                           
+
−
                        </td>
+
−
                        <td>
+
−
                           
+
−
                        </td>
+
−
                       
+
−
+
−
                    </tr>
+
−
                </table>
+
−
            </div>
+
−
</div>
+
 
</div>
 
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Latest revision as of 00:01, 19 September 2015

Lab note
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

 2

 3

 4
5

 6

 7

 8

 9

 10

 11
12

 13

 14

 15

Transformation of
GBP[FadL-GBP]
Cultivation of
OmpA[OmpA+pSB1K3] Kr 		16

Cultivation of
GBP[FadL-GBP]
Plasmid purification of
OmpA[OmpA+pSB1K3] Kr
Digestion of
OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) 		17

Plasmid purification of
GBP[FadL-GBP]
Digestion of
GBP[FadL-GBP] (EcoRI and PstI)
Electrophoresis of
digested product:
GBP[FadL-GBP] (EcoRI and PstI) 		18

Transformation of
ligated product:
GBP[FadL-GBP+pSB1C3] Cr
19

Cultivation of
ligated product: GBP[FadL-GBP] Cr
PCR product: Terminator[J61048] 		20

 21

Cultivation of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+pSB1C3] Cr 		22

Plasmid purification of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+PSB1C3] Cr
Digestion of
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI)
Electrophoresis of
digested product:
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI) 		23

Digestion of OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI)
Electrophoresis of
digested product:
OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI) 		24

Digestion of
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI) 		25

Electrophoresis of
digested product:
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI)
Digestion of
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Electrophoresis of
digested product:
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
26

Ligation of
RFP[E1010+B0034+pSB1K3] Kr
Transformation
of ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[FadL-GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
Electrophoresis of
digested product:
RFP[E1010+J61048+pSB1K3] Kr
[J23101+B0034] 		27

Digestion of
Terminator[J61048] Ar (XbaI and PstI)
PCR of
ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr 		28

Electrophoresis of
RFP[E1010+J61048+pSB1K3] Kr
OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of
ligated product:
GBP[B0034+FadL-GBP+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr PCR of
ligated product
OmpA[J23101+B0034+OmpA] Ar 		29

Cultivation of
RFP[E1010+J61048+pSB1K3] Kr PCR of
OmpA[J23101+B0034+OmpA] Ar
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar Ligation of
GBP[FadL-GBP+J61048+pSB1K3] Kr 		30

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr Ligation of
OmpA[J23101+B0034+OmpA] Ar PCR of
OmpA[J23101+B0034+OmpA] Ar Transformation of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR of
Transformation product:
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
GBP[B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar 		2
3

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		4

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
5/4 Transformation off ligated products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
RFP[E1010+J61048] (Kr) (XbaI and PstI)
PCR of ligated products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		5

Electrophoresis of
digested products:
RFP[E1010+J61048] (Kr) (XbaI and PstI)
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI)
GBP[FadL-GBP+J61048] Kr (EcoRI and PstI)
PCR of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
Cultivation of PCR product:
RFP[E1010+J61048+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		6

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Cultivation of PCR product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
PCR of backbone:
[pSB1C3] Cr
[pSB1A3] Ar
[pSB1K3] Kr
Electrophoresis of
PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] Ar
digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) 		7

Digestion of
RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI)
RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI)
[pSB1C3] Cr (EcoRI and PstI)
[pSB1A3] Ar (EcoRI and PstI)
[pSB1K3] Kr (EcoRI and PstI)
OmpA[J23101+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI)
Plasmid purification of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
[pSB1K3] Kr
Electrophoresis of digested products:
RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI)

RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI)
[pSB1C3] Cr (EcoRI and PstI)
[pSB1A3] Ar (EcoRI and PstI)
[pSB1K3] Kr (EcoRI and PstI)
Ligation of
RFP[B0034+E1010+J61048+pSB1C3] Cr 		8

Electrophoresis of digested products:
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI)
Transformation off ligated products:
RFP[B0034+E1010+J61048+pSB1C3] Cr
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Transformation off
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar 		9

PCR of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar
Electrophoresis of PCR products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
[B0030] Ar
[J23110] Ar
Cultivation of
[B0030] Ar
[J23110] Ar
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
10

Electrophoresis of
[B0030] Ar
Cultivation of
[B0030] Ar

Plasmid purification of
[J23110] Ar
[B0030] Ar
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
[B0030] Ar (EcoRI and SpeI)
[J23110] Ar (EcoRI and SpeI)
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
Electrophoresis of digested products:
[B0030] Ar (EcoRI and SpeI)
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) 		11

Electrophoresis of digested products:
[J23110] Ar (EcoRI and SpeI)
Ligation of
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr
Transformation off
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr
PCR of
RFP[B0030+E1010+J61048+pSB1C3] Cr
GFP[B0030+E0040+J61048+pSB1K3] Kr 		12

PCR of ligated products:
RFP[B0030+E1010+J61048+pSB1C3] Cr
Cultivation of PCR products:
RFP[B0030+E1010+J61048+pSB1C3] Cr
Plasmid purification of
RFP[B0030+E1010+J61048+pSB1C3] Cr
Digestion of
RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI)
RFP[B0030+E1010+J61048] Cr (EcoRI and PstI)
Electrophoresis of
digested products:
RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI)
RFP[B0030+E1010+J61048] Cr (EcoRI and PstI)
PCR products: RFP[B0030+E1010+J61048+pSB1C3] Cr 		13

Ligation of
[J23110+B0034+pSB1K3] Kr 		14

Cultivation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
Transformation off
[J23110+B0034+pSB1K3] Kr
Electrophoresis of digested products:
GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
[pSB1K3] Kr (EcoRI and PstI) 		15

Digestion of
GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		16

Transformation off
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI)
Ligation of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Transformation off
[J23110+B0034+pSB1K3] Kr
17

Ligation of
[J23110+B0034+pSB1K3] Kr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr
PCR of
GBP[B0034+FadL-GBP+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of PCR products:
GBP[B0034+FadL-GBP+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr 		18

Cultivation of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Plasmid purification of
GBP[B0034+FadL-GBP+pSB1C3] Cr
Digestion of
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
GFP[B0030+E0040+J61048] Transformation off
[J23110+B0034+pSB1K3] Kr
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
GFP[B0030+E0040+J61048] 		19

Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
digested products:
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Transformation off
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
GFP[B0030+E0040+J61048]
Cultivation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		20

Cultivation of
OmpA[J23101+B0034+OmpA] Ar
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Plasmid purification of
OmpA[J23101+B0034+OmpA] Ar
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Digestion of
OmpA[J23101+B0034+OmpA] Ar (EcoRI and Pst1)
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr (EcoRI and PstI)
OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI)
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Ligation of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Transformation off
[J23110+B0034+pSB1K3] Kr
[pSB3C5] 抗性
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI) 		21

Digestion of
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI)
scFv-B[scFv-B] Ar (EcoRI and PstI)
scFv-C[scFv-C] Ar (EcoRI and PstI)
Ligation of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr 		22

Cultivation of
[pSB3C5] Cr
[J23110+B0034+pSB1K3] Kr
Transformation off
GBP[J23110+B0034+GBP] Ar
PCR of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Electrophoresis of
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+pSB1K3] Kr
scFv-C[scFv-C+pSB1K3] Kr
Ligation of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
Transform of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar 		23

Plasmid purification of
[pSB3C5] Cr
[J23110+B0034+pSB1K3] Kr
Digestion of
[pSB3C5] Cr (EcoRI and PstI)
[J23110+B0034+pSB1K3] Kr (EcoRI and PstI)
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Transformation off
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
24

Electrophoresis of digested products:
[pSB3C5] Cr (EcoRI and PstI)
[J23110+B0034+pSB1K3] Kr (EcoRI and PstI)
Cultivation of
PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1A3] Ar
Plasmid purification of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
GFP[B0030+E0040+J61048]
Digestion of
[J23101+B0034] Kr (EcoRI and SpeI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
PCR of
GBP[J23110+B0034+GBP] Ar by two pArts
Electrophoresis of
GBP[J23110+B0034+GBP] Ar 		25

Transformation off
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Cultivation of
GBP[J23110+B0034+FadL-GBP] Ar
Digestion of
GBP[B003+FadL-GBP] Cr (XbaI and PstI)
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI)
Electrophoresis of
GFP[B0030+E0040+J61048]
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI)
Cultivation of
scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI)
scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI)
GFP[B0030+E0040+J61048] (XbaI and PstI) 		26

Ligation of
OmpA[J23110+B0034+OmpA+pSB1A3] Ar
GBP[J23110+B0034+GBP+pSB1A3] Ar
Gel extraction of
[J23110] Ar (SpeI and PstI)
Plasmid purification of
GBP[J23110+B0034+GBP] Ar by two pArts
Digestion of
GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI) 		27

PCR of
[J23101+B0034+OmpA+pSB3C5]
Electrophoresis of
GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI)
GBP[B0034+FadL-GBP] Cr (XbaI and PstI)
[J23110] Ar (SpeI and PstI) 		28

Ligation of
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性??
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
scFv-B[scFv-B+pSB1A3] Ar
scFv-C[scFv-C+pSB1A3] Ar
PCR of
[J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S)
OmpA[J23101+B0034+OmpA+pSB3C5] Cr

Plasmid purification in gel slices of PCR products:
OmpA[EX-OmpA-S]
Gel extraction of
OmpA[OmpA] (EcoRI and SpeI)
Transformation off
OmpA[J23101+B0034+OmpA+pSB3C5] 		29

Plasmid purification of
[J23110] Ar
[pSB1C3]
Digestion of
[J23110] Ar (EcoRI and SpeI)
[pSB1C3] (EcoRI and SpeI)
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
Electrophoresis of
[J23110] Ar (EcoRI and SpeI)
[pSB1C3] (EcoRI and SpeI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr 		30

PCR of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
[J23110+B0034+pSB1K3] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
31

Cultivation of
[J23110+B0034] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
Plasmid purification of
[J23110+B0034] Kr
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar
Digestion of
[J23110+B0034] Kr (EcoRI and PstI)
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI)
[J23110+B0034] (EcoRI and SpeI) Kr
[J23101+B0034] (EcoRI and SpeI) Kr
OmpA[OmpA] Cr (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
Electrophoresis of
[J23110+B0034] Kr (EcoRI and PstI)
GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI)
Ligation of
OmpA[OmpA+pSB1C3] Cr
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Electrophoresis of
[J23110+B0034] (EcoRI and SpeI) Kr
[J23101+B0034] (EcoRI and SpeI) Kr
OmpA[OmpA] (NcoI and PstI)
GFP[K1489002+E0040] Kr
Digestion of
[pSB1C3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
OmpA[OmpA] (EcoRI and NcoI)
[pSB3C5] (EcoRI and PstI)
Ligation of
GFP[J23101+B0034+K1489002+E0040+pSB3C5] (Cr)
GFP[J23110+B0034+K1489002+E0040+pSB3C5] (Cr)
Electrophoresis of digested product:
[pSB1C3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
OmpA[OmpA] (EcoRI and NcoI)
Cultivation of
BFP[OmpA+K592100]
PCR of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr
Electrophoresis of
GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		2

PCR of
OmpA[EX-OmpA-S+pSB1C3] Cr
Electrophoresis of
OmpA[EX-OmpA-S+pSB1C3] Cr
OmpA[OmpA] (EcoRI and NcoI)
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Digestion of
[pSB1A3] (EcoRI and NcoI)
Electrophoresis of digested products:
[pSB1A3] (EcoRI and NcoI) 		3

Ligation of
OmpA[OmpA+psB1C3] Cr
[J23110+B0034+pSB1K3] Kr
Transform of
OmpA[OmpA+psB1C3] Cr
[J23110+B0034+pSB1K3] Kr
Digestion of
BFP[OmpA+ K592100] (NcoI and PstI)
OmpA[OmpA] (NcoI and PstI) 		4

Cultivation of
[J23110+pSB1A3] Ar
Transform of
[pSB1C3]
Digestion of
[pSB1C3] (EcoRI and PstI)
Ligation of
OmpA[OmpA+pSB1C3] Cr
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Transform of
[B0034+pSB1A3] Ar 		5

Transform of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
Plasmid purification of
[J23110+pSb1A3] Ar
Digestion of
[J23110+pSb1A3] Ar
PCR of
[J23110+pSb1A3] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Transform of
OmpA[OmpA+pSB1C3] Cr
Electrophoresis of
[pSB1C3]
[B0034+pSB1A3] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		6

Cultivation of
[pSB1C3]
Digestion of
[pSB1C3] (EcoRI and SpeI)
7

Gel extraction of
OmpA[EX-OmpA-S] 抗性
[pSB1C3] (EcoRI and SpeI)
Gel extraction of
[pSB1C3] (EcoRI and SpeI)
Transform of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
[pSB1A3]
OmpA[J23101+B0034+OmpA] Ar
Ligation of
OmpA[OmpA+pSB1C3] Cr
Cultivation of
[pSB1C3] 		8

Transform of
OmpA[OmpA+pSB1C3] Cr
[pSB3K3] [
pSB6A1]
Plasmid purification of
[pSB1C3]
Digestion of
OmpA[EX-OmpA-S] (EcoRI and SpeI)
Electrophoresis of
[pSB1C3] (EcoRI and SpeI)
Transform of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr 		9

Ligation of
OmpA[OmpA+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Cultivation of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Plasmid purification of
[pSB3K3]
[pSB6A1]
Digestion of
[pSB3K3] (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI) 		10

Transform of
[OmpA+pSB1C3] Cr
Plasmid purification of
[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Digestion of
[J23101+B0034+OmpA+K592100] Cr (EcoRI and PstI)
Electrophoresis of
[pSB3K3] (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI)
Transform of
[J23110+B0034] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3]
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Electrophoresis of
[pSB3K3]
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		11

Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
[J23110+B0034+pSB1C3] Cr
Ligation of
[J23110+B0034+pSB1K3] Kr
[J23110+B0034+pSB1C3] Cr
PCR of
OmpA[OmpA+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Electrophoresis of
OmpA[OmpA+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Cultivation of
OmpA[OmpA+pSB1C3] Cr 		12

PCR of
[J23110+B0034+pSB1C3] Cr
[B0034+pSB1A3] Ar
Plasmid purification of
OmpA[OmpA+pSB1C3] Cr
Ligation of
[J23110+B0034+pSB1C3] Cr
Transform of
[J23110+B0034+pSB1C3] Cr 		13

Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
14

SDS-page of
[pSB1C3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
[pSB3C5]
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
scFv-B[OmpA+scFv-B]
scFv-C[OmpA+scFv-C]
Electrophoresis of
[J23110]
[B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI)
Ligation of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Cr
Digestion of
OmpA[OmpA+pSB1C3] Cr (EcoRI and SpeI)
OmpA[OmpA+pSB1C3] Cr (EcoRI and PstI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Ligation of
GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Transform of
GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar 		15

 16

Transform of
[pSB3C5] 		17

Cultivation of
[pSB3C5] 		18

Plasmid purification of
[pSB3C5]
Digestion of
[pSB3C5]( EcoRI and PstI)
Electrophoresis of
[pSB3C5]( EcoRI and PstI)
Ligation of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		19

Transform of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		20

PCR of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
21

 22

 23

PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Cultivation of
[pSB3C5]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
BFP[OmpA+K592100]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1]
[psB6A1]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		24

Plasmid purification of
[pSB3C5] 		25

Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Transform of
[J23101+B0034+pSB1K3] Kr 		26

 27

Digestion of
OmpA[OmpA] Cr (EcoRI and PstI)
Electrophoresis of
GBP[FadL-GBP] 抗性 (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
28

Cultivation of
BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
[pSB3C5]
[pSB1C3]
[pSB1A3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
[J23101+B0034] Kr
GBP[FadL-GBP] 抗性 (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
BFP[J23101+B0034+OmpA+K592100] Ar (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr (EcoRI and PstI)
[pSB6A1] (EcoRI and PstI)
OmpA[OmpA] Cr (XbaI and PstI)
[E0040] Ar (XbaI and PstI)
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
[B0034+E0040+pSB1K3] Kr
GBP[FadL-GBP+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
Digestion of
BFP[J23101+B0034+OmpA+K592100] Cr (XbaI and PstI)
SDS-page of
[pSB1A3]
[pSB1C3]
BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
[pSB3C5]
[pSB6A1]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		29

PCR of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		30

Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Cultivation of
[pSB6A1]
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Digestion of
GBP[]FadL-GBP] 抗性 (EcoRI and PstI) 		2

Digestion of
[J23110] Ar (SpeI and PstI)

Plasmid purification in gel slices of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		3

Gel extraction of
[J23110] Ar (SpeI and PstI)
[pSB6A1] (EcoRI and PstI)
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
GBP[FadL-GBP] (EcoRI and Pstl)
Ligation of
GBP[FadL-GBP+pSB3C5] Cr
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar 		4

Transform of
GBP[FadL-GBP+pSB3C5] Cr
GBP[FadL-GBP+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
[J23101+B0034+pSB1K3] Kr
5

Plasmid purification of
[J23101+B0034+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		6

Cultivation of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar 		7

Digestion of
[J23101+B0034+pSB1K3] Kr (EcoRI and PstI)
SDS-page of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
[pSB1A3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
Ligation of
[J23110+B0034] Ar
Electrophoresis of
[J23101+B0034+pSB1K3] Kr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		8

Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Transform of
[J23110+B0034] Ar
BFP[J23101+B0034+OmpA+K592100]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100]
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		9

Electrophoresis of
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] Cr
[pSB3C5]
BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] Ar
Cultivation of
scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		10

Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		11

PCR of
[J23110+B0034] Ar
Electrophoresis of
[J23110+B0034] Ar
12

Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
[J23110+B0034] Ar
Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		13

Plasmid purification of
[J23110+B0034] Ar
Transform of
[R0010]
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		14

Digestion of
[J23110+B0034] Ar (EcoRI and PstI)
GBP[FadL-GBP] Kr (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and SpeI)

Electrophoresis of
[J23110+B0034] Ar (EcoRI and PstI)
GBP[FadL-GBP] Kr (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and SpeI)
Transform of
[R0010] Cr
Cultivation of
[pSB1C3]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		15

Digestion of
[pSB6A1]
[pSB3C5]
Anti-Her 2[Herceptin+E1010]
[J23101+B0034+OmpA+scFv-C]
[J23101+B0034+OmpA+scFv-B]
Cultivation of
[pSB6A1]
[R0010]
PCR of
OmpA[OmpA-linker] (EcoRI and PstI)
Transform of
[J23110+B0034] Kr
BFP[K592100+J61048] Kr 		16

Gel extraction of
OmpA[OmpA-linker] (EcoRI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
Digestion of
OmpA[OmpA-linker] (EcoRI and PstI)
Transform of
OmpA[J23101+B0034+OmpA+pSB3C5] Cr 		17

Electrophoresis of
OmpA[OmpA-linker] (EcoRI and PstI)
Anti-Her 2[Herceptin+E1010] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		18

Ligation of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
19

Transform of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		20

 21

 22

 23

 24

Transform of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
PCR of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
Electrophoresis of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		25

Cultivation of
[R0010] Cr
BFP[K592100+J61048] Kr
PCR of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
Electrophoresis of
OmpA[OmpA-linker+pSB1C3] Cr
Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
26

Plasmid purification of
[R0010] Cr
BFP[K592100+J61048] Kr
Digestion of
[R0010] Cr (EcoRI and SpeI)
BFP[K592100+J61048] Kr (EcoRI and PstI)
Electrophoresis of
[R0010] Cr
BFP[K592100+J61048] Kr
Ligation of
[R0010+B0034+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
BFP[B0030+K592100+J61048+pSB1C3] Cr
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
Ligation of
OmpA[OmpA-linker+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
Cultivation of
[pSB1C3]
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar 		27

PCR of
GBP[FadL-GBP]
Anti-Her 2[Herceptin+E1010]
Gel extraction of
GBP[FadL-GBP]
Anti-Her 2[Herceptin+E1010]
Transform of
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3]
BFP[B0030+K592100+J61048+pSB1C3] Cr
PCR of
GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr
Gel extraction of
GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr
SDS-page of
[pSB1C3]
GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] Cr
GBP[FadL-GBP+pSB6A1] Ar
[pSB6A1] 		28

Ligation of
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr
BFP[B0030+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
PCR of
BFP[B0030+K592100+J61048+pSB1C3] Cr
[R0010+B0034+pSB1K3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr
OmpA[J23110+B0034+OmpA+pSB3C5] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
SDS-page of
[pSB1C3]
BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
[pSB6A1]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
Transform of
OmpA[OmpA-linker] Cr 		29

Electrophoresis of
OmpA[J23101+B0034+OmpA+pSB3C5]
Transform of
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar
PCR of
BFP[B0034+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Electrophoresis of
BFP[B0034+K592100+J61048+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Digestion of
Anti-Her 2[Herceptin-own] (EcoRI and PstI)
Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
GBP[GBP] (EcoRI and PstI)
Ligation of
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar
GFP[R0010+B0030+E0040+J61048] Ar
Electrophoresis of
Anti-Her 2[Herceptin-own] (EcoRI and PstI)
Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
GBP[GBP] (EcoRI and PstI)
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar
GFP[R0010+B0030+E0040+J61048] Ar 		30

Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr
Ligation of
GBP[FadL-GBP+pSB1C3]Cr
Digestion of
Anti-Her 2[Herceptin own] (NcoI and PstI)
[pSB1A3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Cultivation of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
[J23101+B0034] Kr
Digestion of
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
Electrophoresis of
Anti-Her 2[Herceptin own] (NcoI and PstI)
[pSB1A3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Plasmid purification of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
[J231-1+B0034] Kr
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI)
[J231-1+B0034] Kr (EcoRI and SpeI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI) 		31

Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI)
[J231-1+B0034] Kr (EcoRI and SpeI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Ligation of
Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar
Transform of
Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar
Electrophoresis of
[pSB1C3] (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Digestion of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Electrophoresis of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
2

Electrophoresis of
[pSB1C3] (XbaI and PstI)
OmpA[OmpA] (EcoRI and PstI)
scFv-B[scFv-B] Kr (NcoI and PstI)
scFv-C[scFv-C] Kr (NcoI and PstI)
OmpA[OmpA] (NcoI and PstI)
Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI) 		3

Transform of [R0010+B0034+pSB1K3] Kr
GBP[FadL-GBP+pSB1C3] Cr
BFP[B0030+K592100+J61048] Cr
Digestion of
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		4

Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI)
PCR of
[R0010+B0034+pSB1K3] Kr
BFP[B0030+K592100+J61048] Cr
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		5

Digestion of
[pSB6A1] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Cultivation of
BFP[B0030+K592100+J61048] Cr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
GBP[FadL-GBP] Cr
BFP[OmpA+K592100] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Ar
scFv-C[scFv-C+pSB1C3] Cr
scFv-B[scFv-B+oSB1C3] Cr
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr (EcoRI and PstI)
Transform of
GBP[J23101+B0034+FadL-GBP]Cr
scFv-B[scFv-B+pSB1C3] Cr
scFv-C[scFv-C+pSB1C3] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr
Gel extraction of
[pSB6A1] (EcoRI and PstI)
[OmpA] (NcoI and PstI)
[pSB1C3] (EcoRI and PstI) 		6

Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Ligation of
Anti-Her 2[OmpA+Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
Transform of
Anti-Her 2[OmpA+Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
[pSB1C3]
[pSB1A3]
[pSB1K3]
Plasmid purification of
BFP[B0030+K592100+J61048]
Digestion of
BFP[B0030+K592100+J61048] (EcoRI and PstI)
Cultivation of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Ligation of
[R0010+B0034] Kr
GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] Cr
Digestion of
[pSB1C3] (XbaI and PstI)
Electrophoresis of
GBP[J23101+B0034+FadL-GBP] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr
scFv-C[scFv-C] Cr
scFv-B[scFv-B] Cr
Plasmid purification of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Digestion of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
[B0034]
[pSB1C3]
Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Transform of
GBP[J23101+B0034+FadL-GBP] Ar
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr
[pSB1C3] (XbaI and PstI)
[pSB6A1] (XbaI and PstI)
[pSB1C3] (XbaI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr
GFP[J23101+B0034+E0040+J61048] Ar
SDS-page of
GFP[J23101+B0034+E0040+J61048] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Ar
[pSB6A1]
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Ar
Digestion of
[pSB1C3] (XbaI and PstI)
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Ligation of
[pSB1C3] (XbaI and PstI)
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[Herceptin-Own] Cr
BFP[J23101+B0034+OmpA+K592100] Cr 		7

Plasmid purification of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr
PCR of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Cultivation of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
Plasmid purification of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B] Cr
scFv-C[J23101+B0034+OmpA+scFv-C] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
OmpA[OmpA] (NcoI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
Anti-Her 2[OmpA+Herceptin-Web] C
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI)
Anti-Her 2[Herceptin-Web] (NcoI and PstI)
Anti-Her 2[Herceptin-Own] (NcoI and PstI)
Transform of
scFv-C[OmpA+scFv-C] Cr
scFv-B[OmpA+scFv-B] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[OmpA+Herceptin-Own] Cr
Anti-Her 2[OmpA+Herceptin-Web] C
BFP[J23101+B0034+OmpA+K592100] Cr 		8

Plasmid purification of
scFv-C[J23101+OmpA+scFv-C] Cr
scFv-B[J23101+OmpA+scFv-B] Cr
Digestion of
scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI)
scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Cultivation of
[pSB1K3]
[pSB1C3]
[pSB1A3]
PCR of
BFP[J23101+B0034+OmpA+K592100] Cr
Transform of
scFv-C[OmpA+scFv-C] Cr
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100] Cr
scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI)
scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Digestion of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and SpeI)
OmpA[OmpA] Cr NcoI and PstI
OmpA[OmpA+K592100] Cr NcoI and PstI
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr
Digestion of
Anti-Her 2[Herceptin-Own] (EcoRI and PstI)
Anti-Her 2[Herceptin-Web] (EcoRI and PstI)
Electrophoresis of
BFP[J23101+B0034+OmpA+K592100] Cr
9

Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar
Plasmid purification of
[pSB1C3]
[pSB1K3]
[pSB1A3]
Digestion of
[pSB1C3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1A3] (EcoRI and PstI)
Transform of
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr
[R0010+B0034] Cr
BFP[J23101+B0034+OmpA+K592100] Cr
Electrophoresis of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr (EcoRI and SpeI)
OmpA[OmpA] Cr (NcoI and PstI)
Digestion of
[pSB1A3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
RFP[B0030+E1010+J61048] Cr (XbaI and PstI)
Electrophoresis of
scFv-C[OmpA+scFv-C] Kr
PCR of
GBP[GBp+pSB1C3] Cr
Digestion of
BFP[B0030+K592100+J61048] Cr (XbaI and PstI) 		10

Electrophoresis of
GBP[GBp+pSB1C3] Cr
[pSB1A3] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] Kr
Cultivation of
[R0010+B0034] Cr
BFP[B0030+K592100+J61048] Cr
scFv-C[OmpA+scFv-C] Kr
Digestion of
[pSB1C3] (EcoRI and Pstl)
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr
Gel extraction of
[pSB1C3] (EcoRI and PstI)
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web]
Anti-Her 2[Herceptin-Web]
Anti-Her 2[Herceptin-Own] 		11

Plasmid purification of
[R0010+B0034] Cr
scFv-C[OmpA+scFv-C] Kr
BFP[OmpA+K592100] Cr

Gel extraction of
[pSB1C3] (EcoRI and PstI)
Digestion of
[R0010+B0034] Cr (EcoRI and SpeI)
scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI)
BFP[OmpA+K592100] Cr (EcoRI and PstI)
GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI)
Ligation of
Anti-Her 2[Herceptin-Web] Cr
PCR of
scFv-B[OmpA+scFv-B] Kr
Electrophoresis of
[R0010+B0034] Cr (EcoRI and SpeI)
scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI)
BFP[OmpA+K592100] Cr (EcoRI and PstI)
GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI)
Ligation of
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Electrophoresis of
scFv-B[OmpA+scFv-B] Kr
PCR of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Transform of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr 		12

Electrophoresis of
scFv-B[OmpA+scFv-B] Kr
Transform of
Anti-Her 2[Herceptin-Web] Cr
Anti-Her 2[Herceptin-Own] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar
PCR of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Digestion of
[pSB1C3] (EcoRI and PstI)
Electrophoresis of
scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		13

Transform of
GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar
Cultivation of
[pSB1C3]
scFv-B[OmpA-scFv-B] Kr
Plasmid purification of
[pSB1C3]
scFv-B[OmpA-scFv-B] Kr
Digestion of
[pSB1C3] (EcoRI and PstI)
scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI)
Electrophoresis of
[pSB1C3] (EcoRI and PstI)
Ligation of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Electrophoresis of
[pSB1C3] (EcoRI and PstI)
scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI) 		14

Transform of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		15

PCR of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr
PCR of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Transform of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Electrophoresis of
GBP[FadL-GBP] (EcoRI and PstI)
16

PCR of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr
Cultivation of
OmpA[R0010+B0034+OmpA]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr 		17

Ligation of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		18

Plasmid purification of
OmpA[R0010+B0034+OmpA+pSB1A3] Ar
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
Digestion of
BFP[OmpA+K592100] Cr (EcoRI and NcoI)
Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI)
BFP[B0030+K592100+J61048] Cr (XbaI and PstI)
OmpA[R0010+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI)
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI)
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar
GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] Cr
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+FadL-GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
Ligation of
scFv-B[R0010+B0034+OmpA+scFv-B]
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
Anti-Her 2[Herceptin-Web]
scFv-B[J23101+B0034+OmpA+scFv-B]
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Anti-Her 2[Herceptin-Own]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		19

Electrophoresis of
Anti-Her 2[Herceptin-Web]
scFv-B[J23101+B0034+OmpA+scFv-B]
scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr
Anti-Her 2[Herceptin-Own]
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
scFv-C[OmpA+scFv-C]
Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI)
BFP[B0030+K592100+J61048] Cr (XbaI and PstI)
Transform of
GBP[R0010+B034+FadL-GBP] Ar
GBP[J23101+B0034+FadL-GBP] Ar
GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr
PCR of
scFv-B[J23110+B0034+OmpA+scFv-B] 		20

PCR of
GBP[FadL-GBP] Kr
OmpA[OmpA] Cr
Gel extraction of
OmpA[OmpA] (EcoRI and SpeI)
Plasmid purification of
Anti-Her 2[Herceptin-Own] Cr
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar
PCR of
GBP[FadL-GBP] Kr
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048]
scFv-B[R0010+B0034+OmpA+scFv-B]
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
Digestion of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI)
Anti-Her 2[Herceptin-Own] Cr
Electrophoresis of
OmpA[R0010+B0034+OmpA+pSB1C3] Cr
GBP[R0010+B0034+GBP+pSB1C3] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048]
Ligation of
scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] Kr
GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] Kr
PCR of
GBP[R0010+B0034+FadL-GBP]
GBP[J23110+B0034+FadL-GBP+B0030+E0040] 		21

Electrophoresis of
[pSB1K3] (EcoRI and PstI)
[R0010] Cr (EcoRI and SpeI)
[J23110] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] Ar (EcoRI and SpeI)
BFP[B0034+K592100+J61048] Cr (XbaI and PstI)
RFP[B0034+E1010+J61048] Cr (XbaI and PstI)
GBP[B0034+FadL-GBP+B0030+J61048] Cr (XbaI and PstI)
Ligation of
BFP[J23110+B0034+K592100+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+K592100+E1010+J61048pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3]
Transform of
scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048pSB1A3] Ar
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] Kr
PCR of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
GBP[J23101+B0034+FadL-GBP]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040] 		22

Digestion of
[pSB1C3] (XbaI and SpeI)
PCR of
scFv-B[R0010+B0034+OmpA+scFv-B] Kr
scFv-C[R0010+B0034+OmpA+scFv-C] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-web] Kr
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] Ar
GBP[FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr
BFP[J23110+B0034+K592100+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
Cultivation of
[pSB6A1]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
Electrophoresis of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[FadL-GBP] (XbaI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Digestion of
[pSB1C3] (XbaI and PstI)
Ligation of
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3]
GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Ar
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048]
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E0040+J61048]
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+K592100+J61048]
scFv-C[R0010+B0034+OmpA+scFv-C]
Cultivation of
GBP[R0010+B0034+FadL-GBp+B0030+E0040+J61048] Cr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Ar
23

Transform of
GBP[FadL-GBP] Ar
OmpA[OmpA] Cr
Plasmid purification of
Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] Kr
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] Kr
BFP[J23101+B0034+K592100+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP]
Anti-Her 2[R0010+B0034+Herceptin-own]
Transform of
Anti-Her 2[R0010+B0034+Herceptin-web+B0030+E1010+J61048] Kr
Anti-Her 2[R0010+B0034+Herceptin-own+B0030+E1010+J61048] Kr
BFP[J23101+B0034+K592100+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Cultivation of
GBP[R0010+B0034+FadL-GBP]
GBP[J23110+B0034+fadL-GBP+B0030+E0040]
scFv-B[R0010+B0034+OmpA+scFv-B] Kr
Electrophoresis of
scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and SpeI)
GBP[B0034+FadL-GBP+B0030+E0040+J61048] (XbaI and PstI)
RFP[B0030+E1010+J61048] (XbaI and PstI)
BFP[J23101+B0034+K592100+J61048]
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Anti-Her 2[R0010+B0034+Herceptin-web] (EcoRI and PstI)
Anti-Her 2[R0010+B0034+Herceptin-own] (EcoRI and PstI)
GBP[R0010+B0034+FadL-GBP+B0030+E0040] (EcoRI and PstI)
GBP[R0010+B0034FadL-GBP] (EcoRI and PstI)
[pSB1C3] (XbaI and PstI) 		24

Ligation of
scFv-B[OmpA+scFv-B] Kr
scFv-C[OmpA+scFv-C] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
Anti-Her 2[OmpA+Herceptin-web] Kr
Digestion of
Anti-Her 2[Herceptin-own] (NcoI and PstI)
Electrophoresis of
GBP[FadL-GBP] Cr (EcoRI and PstI)
GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
GBP[J23110+B0034+FadL-GBP] (EcoRI and SpeI)
GBP[FadL-GBP] (EcoRI and PstI)
Plasmid purification of
GBP[R0010+B0034+FadL-GBP] Ar
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cr
Digestion of
GBP[R0010+B0034+FadL-GBP] Ar (EcoRI and SpeI)
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cr (EcoRI and PstI)
Transform of
GBP[FadL-GBP] Cr
OmpA[OmpA] Cr
PCR of
scFv-B[J23110+B0034+scFv-B+B0030+E1010+J61048+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+E0040+J61048+pSB1K3] Kr
scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+E1010+J61048+pSB1K3] Kr
Anti-Her 2[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] Kr
scFv-B[J23110+B0034+Herceptin-own+K592100+J61048+pSB1K3] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
Transform of
scFv-B[OmpA+scFv-B] Kr
scFv-C[OmpA+scFv-C] Kr
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+J61048] Kr
Anti-Her 2[OmpA+Herceptin-web] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
SDS-page of
scFv-C[J23110+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+E0040+J61048]
GFP[J23110+B0034+E0040+J61048
GBP[R0010+B0034+FadL-GBP]
[pSB1C3]
OmpA[R0010+B0034+OmpA+J61048]
GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
PCR of
GBP[FadL-GBP] Cr
OmpA[OmpA]
Ligation of
BFP[J23101+B0034+OmpA+K592100] Cr
scFv-B[J23101+B0034+OmpA+scFv-B+] Cr 		25

Digestion of
[pSB3C5] (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and PstI)
scFv-B[J23101+B0034+OmpA+scFv-B+J61048] Cr (EcoRI and PstI)
scFv-B[R0010+B0034+OmpA+scFv-B] Kr (EcoRI and PstI)
scFv-C[J23101+B0034+OmpA+scFv-C+B030+E0040+J61048] Ar (EcoRI and PstI)
Cultivation of
GFP[J23101+B0034+E0040+J61048] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
PCR of
scFv-B[OmpA+scFv-B]
Anti-Her 2[OmpA+Herceptin-own]
GBP[FadL-GBP] Ar
Transform of
scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048]
scFv-B[J23101+B0034+OmpA+scFv-B +B0030+E0040+J61048]
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
Ligation of
scFv-C[OmpA+scFv-C] Kr
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr
scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048] Kr
Plasmid purification of
scFv-C[R0010+B0034+OmpA+scFv-C]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048]
Digestion of
scFv-C[R0010+B0034+OmpA+scFv-C] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040+J61048] (EcoRI and PstI)
[pSB1K3] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
BFP[R0010+B0034+OmpA+K592100] (NcoI and PstI)
Cultivation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] Ar
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
OmpA[R0010+B0034+OmpA]
Digestion of
BFP[OmpA+K592100] (EcoRI and NcoI) 		26

Cultivation of
[pSB1C3]
GBP[R0010+B0034+FadL-GBP+B0030+E0040] Kr
Ligation of
GBP[FadL-GBP+pSB1C3] Cr
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
Electrophoresis of
[pSB1K3] (EcoRI and PstI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] (EcoRI and SpeI)
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E0040]
scFv-C[R0010+B0034+OmpA+scFv-C]
Digestion of
GBP[FadL-GBP] (XbaI and PstI)
Transform of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+E1010]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own+B0030+K592100]
Digestion of
[J23101] (EcoRI and SpeI)
BFP[OmpA+K592100] (EcoRI and NcoI)
Plasmid purification of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
BFP[R0010+B0034+OmpA+K592100]
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
[pSB1C3]
Digestion of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] (EcoRI and PstI)
BFP[R0010+B0034+OmpA+K592100] (EcoRI and PstI)
scFv-B[OmpA+scFv-B] Kr(EcoRI and PstI I)
Anti-Her 2[OmpA+Herceptin-own] Kr (EcoRI and PstI)
[pSB1C3] 		27

Transform of
GBP[FadL-LGBP] Cr
Plasmid purification of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own]
Ligation of
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] Cr
scFv-C[OmpA+scFv-C] Kr
scFv-B[OmpA+scFv-B] Kr
Anti-Her 2[OmpA+Herceptin-own] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
scFv-B [R0010+B0034+OmpA+scFv-B]
Anti-Her 2[R0010+B0034+OmpA+Herceptin-own]
Digestion of
GFP[B0034+E0040+J61048] (XbaI and PstI)
GBP[R0010+B0034+FadL-GBP] Ar (EcoRI and SpeI)
GFP[J23110+B0034+E0040+J61048] (EcoRI and PstI)
[pSB1C3] (EcoRI and PstI)
Ligation of
GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
GFP[J23110+B0034+E0040+pSB6A1] 		28

PCR of
GBP[FadL-GBP] Cr
PCR of
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E1010+J61048]
scFv-B[J23110+B0034+OmpA+scFv-B+B0030+K592100+J61048]
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+K592100+J61048]
Anti-Her 2[OmpA+Herceptin-own]
scFv-C[J23110+B0034+OmpA+scFv-C]
Anti-Her 2[J23110+B0034+Herceptin-own]
scFv-C[OmpA+scFv-C].
Transform of
Anti-Her 2[J23110+B0034+Herceptin-own]
BFP[B0030+K592100+J61084]
BFP[B0030+K592100+J61084]
BFP[J23101+B0034+OmpA+K592100]
Plasmid purification of
scFv-B[OmpA+scFv-B] Kr
scFv-C[R0010+B0034+OmpA+scFv-C]
Cultivation of
Anti-Her 2[J23110+B0034+Herceptin-own+B0030+E0040+J61048]
Anti-Her 2[J23110+B0034+Herceptin-own+B0030] 		29
30

Plasmid purification of
GBP[FadL-GBP]
Anti-Her 2[J23101+B0034+OmpA+Herceptin-own] 		31


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