Difference between revisions of "Team:IONIS Paris/Project/Game Over"

 
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<h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s"  style="font-family:sans-serif; color:#B00000 " >Game over</h1>
 
<h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s"  style="font-family:sans-serif; color:#B00000 " >Game over</h1>
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<h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>What is the Game Over?</big></h2>
 
<h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>What is the Game Over?</big></h2>
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<div class="row">
 
<div class="row">
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<h4  class="wowload fadeInUp"><i class="fa fa-lightbulb-o" style="color:#b22222"></i> Allowing the end of the game </h4>
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<div class="col-sm-6 wowload fadeInLeft">
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<p align="justify"> The game over concerns the end of one round in the Bio-Console game.<br>
 +
Indeed, you can not play indefinitely with the Bio-Console, and as every funny game something can happen to the hero!
 +
Here, the end of the game is the death of the bacteria. </p>
 +
<p align="justify"> As you know, the goal of the game is simple:  to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening.
 +
The toxins will eliminate our hero that will lose his fluorescence or bioluminescence ... GAME OVER
 +
<br>
 +
Our game over is composed of two parts : Toxin-mediated delivery by photopolymerizable liposomes , clean-up of the microfluidic channel.
 +
</p>
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</div>
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<div class="col-sm-6 wowload fadeInRight">
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<center><br><br>
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<img src="https://static.igem.org/mediawiki/2015/8/87/Bioconsole_wiki.png" alt="parc" width="90%" height=auto/></center>
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</div>
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</div>
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</div>
 
 
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<h4><i class="fa fa-lightbulb-o" style="color:#b22222"></i> Allowing the end of the game </h4>
 
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<p align="justify">Nature has evolved a multiple of photoreceptors able to sense light.
 
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Those systems have provide <font style="color:#66cdaa"><b>synthetic biology tools </b></font>for the precise control of biological functions, offering superior spatial and temporal resolution. The research field focusing on the combination of optical method and genetic has been named «optogenetics» and can lead to the control of gene expression, cell morphology or even signaling pathway thank to light signal.</p>
 
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</br>
 
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<h4><i class="fa fa-bolt" style="color:#b22222"></i> Concept </h4>
 
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<h5> Using thermosensitive liposomes</h5>
 
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<p align="justify"> IN CONSTRUCTION</p>
 
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<h5> Using toxins</h5>
 
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<p align="justify"> IN CONSTRUCTION</p>
 
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<div id="about"  class="container spacer about">
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<h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>Photopolymerizable liposome</big></h2>
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<div class="row">
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<div class="col-sm-6 wowload fadeInLeft">
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<p align="center"> <br><br><br>
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<img src="https://static.igem.org/mediawiki/2015/9/96/LIPOSOME.png" alt="Liposome"  width="500" height=auto>
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</p>
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</div>
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<div class="col-sm-6 wowload fadeInRight">
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<h4><i class="fa fa-circle-o" style="color:#b22222"></i> Toxin-mediated delivery  </h4>
 +
<p align="justify">
 +
In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards.
 +
</p>
 +
<p align="justify">
 +
For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm).
 +
Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids.
 +
<br>
 +
In order to ensure  functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled  “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”.
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</p>
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</div>
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</div>
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<div class="wowload fadeInUp">
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<h4><i class="fa fa-times-circle-o" style="color:#b22222"></i>  BactMan - Game Over  </h4>
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<p align="justify" >
 +
Because of a time issue, we did not  synthesize the liposomes and therefore test the first part of our game over.
 +
According to Yavlovich’s paper and if we follow carefully its liposome formation protocol, this system has a high probability to work efficiently.
 +
<br><br>
 +
Nevertheless, visible-light induced solute leakage from the liposome depends on the spectral properties of entrapped solutes. As we did not test the liposomes with the toxins, we cannot say if the leakage will be important or not. In any case, we do not really have to worry about it since we play upon red light.
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</div>
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<br><br><br>
 
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<i class="fa fa-lightbulb-o  fa-5x"></i><h4>Light signals</h4>
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<i class="fa fa-lightbulb-o  fa-5x"></i><h4>Light signals</h4>
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<i class="fa fa-bolt fa-5x"></i><h4>Liposomes</h4>
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<div class="col-sm-3 col-xs-6">
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<i class="fa fa-gamepad  fa-5x"></i><h4>Bio-Console</h4>
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</div>
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<div class="col-sm-3 col-xs-6">
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<i class="fa fa-undo  fa-5x"></i><h4> Game over</h4>
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<i class="fa fa-gamepad  fa-5x"></i><h4>Bio-Console</h4>
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<i class="fa fa-qq  fa-5x"></i><h4> Game over</h4>
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<div id="about"  class="container spacer about" >
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<h4> References </h4>
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<p align="justify" style="font-size:14px">
 +
<i class="fa fa-angle-right"></i> Yavlovich A, Singh A, Blumenthal R, Puri A. A novel class of photo-triggerable liposomes containing DPPC:DC 8,9PC as vehicles for delivery of doxorubcin to cells. Biochim Biophys Acta - Biomembr. 2011;1808(1):117–26.
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<br>
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<h4><i class="fa fa-question" style="color:#b22222"></i> Why did we choose the photoreceptor VVD ?  </h4>
 
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<p align="justify" align= "right">Contrary to other photoreceptors, VVD is a<font style="color:#66cdaa"> small protein </font>with 150 amino-acids facilitating accurate molecular design and avoiding steric issue.
 
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Moreover, it is a homo-dimer when most of photo-inducible dimer are heterodimer.
 
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In addition, the use of  VVD is easy; and doesn’t need any addition of co-factors: VVD works with <font style="color:#66cdaa"><b>Flavin adenine dinucleotide</b></font> (FAD) which is already abundant in eukaryote and prokaryote cells. (Müller & Weber, 2013; Nihongaki, Suzuki, Kawano, & Sato, 2014)  </p>
 
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project" ><figcaption>
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<h2>PROJECT</h2>
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<figcaption>
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<p>Our reality game using bacteria<br>
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<h2>PROJECT</h2>
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</p>             
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<p>BactMan's Adventures<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Biobricks" >
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<h2>BIOBRICKS</h2>
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<figcaption>
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<p>Our parts of DNA<br>
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<h2>BIOBRICKS</h2>
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</p>             
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<p>Our parts of DNA<br>
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<a href="https://2015.igem.org/Team:IONIS_Paris/Project/Microfluidics" >
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<h2>MICROFLUIDICS</h2>
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<figcaption>
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<p>Our challenge in achieving the Bio-console<br>
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<h2>MICROFLUIDICS</h2>
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</p>             
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<p>Our challenge in achieving the Bio-Console<br>
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</figcaption></a>
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</p>             
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Latest revision as of 00:01, 19 September 2015

Game Over

What is the Game Over?

Allowing the end of the game

The game over concerns the end of one round in the Bio-Console game.
Indeed, you can not play indefinitely with the Bio-Console, and as every funny game something can happen to the hero! Here, the end of the game is the death of the bacteria.

As you know, the goal of the game is simple: to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening. The toxins will eliminate our hero that will lose his fluorescence or bioluminescence ... GAME OVER
Our game over is composed of two parts : Toxin-mediated delivery by photopolymerizable liposomes , clean-up of the microfluidic channel.



parc

Photopolymerizable liposome




Liposome

Toxin-mediated delivery

In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards.

For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm). Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids.
In order to ensure functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”.

BactMan - Game Over

Because of a time issue, we did not synthesize the liposomes and therefore test the first part of our game over. According to Yavlovich’s paper and if we follow carefully its liposome formation protocol, this system has a high probability to work efficiently.

Nevertheless, visible-light induced solute leakage from the liposome depends on the spectral properties of entrapped solutes. As we did not test the liposomes with the toxins, we cannot say if the leakage will be important or not. In any case, we do not really have to worry about it since we play upon red light.




Light signals

Liposomes

Bio-Console

Game over

References

Yavlovich A, Singh A, Blumenthal R, Puri A. A novel class of photo-triggerable liposomes containing DPPC:DC 8,9PC as vehicles for delivery of doxorubcin to cells. Biochim Biophys Acta - Biomembr. 2011;1808(1):117–26.

img01

PROJECT

BactMan's Adventures
team

BIOBRICKS

Our parts of DNA
team

MICROFLUIDICS

Our challenge in achieving the Bio-Console



IONIS Paris team. 2015 Edition