Difference between revisions of "Team:IONIS Paris/Project/Game Over"
| (43 intermediate revisions by 2 users not shown) | |||
| Line 13: | Line 13: | ||
<!-- font awesome --> | <!-- font awesome --> | ||
<link href="//maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet"> | <link href="//maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet"> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<!-- favicon --> | <!-- favicon --> | ||
| Line 33: | Line 25: | ||
<!-- Header Starts --> | <!-- Header Starts --> | ||
| − | + | <div class="banner row" id="banner" style="width:101%;min-height:100%;height: 100vh; "> | |
| − | + | <div class="parallax text-center" style="background-image: url(https://static.igem.org/mediawiki/2015/0/07/Game_over.jpg);min-height:100%;"> | |
| − | + | <div class="parallax-pattern-overlay" style="min-height:100%;height: 100vh;"> | |
| − | + | <div class="container text-center" style="min-height:100%;padding-top:200px;"> | |
| − | <div class="banner row" id="banner" style="width:101%"> | + | |
| − | <div class="parallax text-center" style="background-image: url(https://static.igem.org/mediawiki/2015/0/07/Game_over.jpg)"> | + | |
| − | <div class="parallax-pattern-overlay"> | + | |
| − | <div class="container text-center" style="height: | + | |
<h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s" style="font-family:sans-serif; color:#B00000 " >Game over</h1> | <h1 class="intro wow zoomIn" wow-data-delay="0.4s" wow-data-duration="0.9s" style="font-family:sans-serif; color:#B00000 " >Game over</h1> | ||
| − | |||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<!-- INTRODUCTION --> | <!-- INTRODUCTION --> | ||
<div id="about" class="container spacer about"> | <div id="about" class="container spacer about"> | ||
<h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>What is the Game Over?</big></h2> | <h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>What is the Game Over?</big></h2> | ||
| − | |||
<div class="row"> | <div class="row"> | ||
| − | + | <h4 class="wowload fadeInUp"><i class="fa fa-lightbulb-o" style="color:#b22222"></i> Allowing the end of the game </h4> | |
| − | + | <div class="col-sm-6 wowload fadeInLeft"> | |
| − | + | <p align="justify"> The game over concerns the end of one round in the Bio-Console game.<br> | |
| + | Indeed, you can not play indefinitely with the Bio-Console, and as every funny game something can happen to the hero! | ||
| + | Here, the end of the game is the death of the bacteria. </p> | ||
| + | <p align="justify"> As you know, the goal of the game is simple: to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening. | ||
| + | The toxins will eliminate our hero that will lose his fluorescence or bioluminescence ... GAME OVER | ||
| + | <br> | ||
| + | Our game over is composed of two parts : Toxin-mediated delivery by photopolymerizable liposomes , clean-up of the microfluidic channel. | ||
| + | </p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
| − | + | <div class="col-sm-6 wowload fadeInRight"> | |
| − | <div class="col-sm | + | <center><br><br> |
| − | < | + | <img src="https://static.igem.org/mediawiki/2015/8/87/Bioconsole_wiki.png" alt="parc" width="90%" height=auto/></center> |
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
| − | |||
| − | |||
</div> | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <!-- TEXTE --> | ||
| + | <div id="about" class="container spacer about"> | ||
| + | <h2 class="text-center wowload fadeInUp" style="color:#ff0000"><big>Photopolymerizable liposome</big></h2> | ||
| + | <div class="row"> | ||
| + | <div class="col-sm-6 wowload fadeInLeft"> | ||
| + | <p align="center"> <br><br><br> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/9/96/LIPOSOME.png" alt="Liposome" width="500" height=auto> | ||
| + | </p> | ||
| + | |||
| + | </div> | ||
| + | <div class="col-sm-6 wowload fadeInRight"> | ||
| + | <h4><i class="fa fa-circle-o" style="color:#b22222"></i> Toxin-mediated delivery </h4> | ||
| + | <p align="justify"> | ||
| + | In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards. | ||
| + | </p> | ||
| + | <p align="justify"> | ||
| + | For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm). | ||
| + | Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids. | ||
| + | <br> | ||
| + | In order to ensure functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| − | + | <div class="wowload fadeInUp"> | |
| − | + | <h4><i class="fa fa-times-circle-o" style="color:#b22222"></i> BactMan - Game Over </h4> | |
| − | + | <p align="justify" > | |
| − | + | Because of a time issue, we did not synthesize the liposomes and therefore test the first part of our game over. | |
| − | + | According to Yavlovich’s paper and if we follow carefully its liposome formation protocol, this system has a high probability to work efficiently. | |
| − | + | <br><br> | |
| − | + | Nevertheless, visible-light induced solute leakage from the liposome depends on the spectral properties of entrapped solutes. As we did not test the liposomes with the toxins, we cannot say if the leakage will be important or not. In any case, we do not really have to worry about it since we play upon red light. | |
| − | < | + | </p> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br><br><br> | |
| − | + | <!-- HIGHLIGHT Gameover --> | |
| − | + | <div class="highlight-info"> | |
| − | + | <div class="overlay spacer"> | |
| − | + | <div class="container" > | |
| − | + | <div class="row text-center wowload fadeInDownBig"> | |
| − | + | <CENTER> | |
| + | <div class="col-sm-3 col-xs-6"> | ||
| + | <i class="fa fa-lightbulb-o fa-5x"></i><h4>Light signals</h4> | ||
</div> | </div> | ||
| − | + | ||
| − | + | <div class="col-sm-3 col-xs-6"> | |
| − | <div class="col-sm-6 | + | <i class="fa fa-circle-o fa-5x"></i><h4>Liposomes</h4> |
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | |||
| + | <div class="col-sm-3 col-xs-6"> | ||
| + | <i class="fa fa-gamepad fa-5x"></i><h4>Bio-Console</h4> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-sm-3 col-xs-6"> | ||
| + | <i class="fa fa-undo fa-5x"></i><h4> Game over</h4> | ||
| + | </div> | ||
| + | |||
| + | </CENTER> | ||
</div> | </div> | ||
| − | </div> | + | </div> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | ||
| − | <div id="about" class="container spacer about"> | + | |
| − | < | + | <div id="about" class="container spacer about" > |
| + | <h4> References </h4> | ||
| + | <p align="justify" style="font-size:14px"> | ||
| + | <i class="fa fa-angle-right"></i> Yavlovich A, Singh A, Blumenthal R, Puri A. A novel class of photo-triggerable liposomes containing DPPC:DC 8,9PC as vehicles for delivery of doxorubcin to cells. Biochim Biophys Acta - Biomembr. 2011;1808(1):117–26. | ||
| + | <br> | ||
| − | + | </p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<!--BOXES --> | <!--BOXES --> | ||
| − | |||
<div id="works" class=" clearfix grid"> | <div id="works" class=" clearfix grid"> | ||
| − | + | ||
| − | + | <figure class="effect-oscar" > | |
| − | + | ||
<img src="https://static.igem.org/mediawiki/2015/3/34/Wii.jpg" alt="img01"/> | <img src="https://static.igem.org/mediawiki/2015/3/34/Wii.jpg" alt="img01"/> | ||
| − | <a href="https://2015.igem.org/Team:IONIS_Paris/ | + | <a href="https://2015.igem.org/Team:IONIS_Paris/Description" > |
| − | + | <figcaption> | |
| − | + | <h2>PROJECT</h2> | |
| − | + | <p>BactMan's Adventures<br> | |
| − | + | </p> | |
| + | </figcaption> | ||
| + | </a> | ||
</figure> | </figure> | ||
| − | + | <figure class="effect-oscar"> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <figure class="effect-oscar | + | |
<img src="https://static.igem.org/mediawiki/2015/thumb/1/18/Brick-wall.png/800px-Brick-wall.png" alt="team"/> | <img src="https://static.igem.org/mediawiki/2015/thumb/1/18/Brick-wall.png/800px-Brick-wall.png" alt="team"/> | ||
| − | <a href="https://2015.igem.org/Team:IONIS_Paris/Biobricks" ><figcaption> | + | <a href="https://2015.igem.org/Team:IONIS_Paris/Biobricks" > |
| − | + | <figcaption> | |
| − | + | <h2>BIOBRICKS</h2> | |
| − | + | <p>Our parts of DNA<br> | |
| − | + | </p> | |
| + | </figcaption> | ||
| + | </a> | ||
</figure> | </figure> | ||
| − | + | <figure class="effect-oscar"> | |
| − | + | ||
| − | + | ||
| − | <figure class="effect-oscar | + | |
<img src="https://static.igem.org/mediawiki/2015/1/18/GRID.jpg" alt="team"/> | <img src="https://static.igem.org/mediawiki/2015/1/18/GRID.jpg" alt="team"/> | ||
| − | <a href="https://2015.igem.org/Team:IONIS_Paris/Project/Microfluidics" ><figcaption> | + | <a href="https://2015.igem.org/Team:IONIS_Paris/Project/Microfluidics" > |
| − | + | <figcaption> | |
| − | + | <h2>MICROFLUIDICS</h2> | |
| − | + | <p>Our challenge in achieving the Bio-Console<br> | |
| − | + | </p> | |
| + | </figcaption> | ||
| + | </a> | ||
</figure> | </figure> | ||
| − | |||
</div> | </div> | ||
| − | + | <br><br><br> | |
| − | <br><br><br> | + | |
| − | + | ||
| − | + | ||
<!-- The Bootstrap Image Gallery lightbox, should be a child element of the document body --> | <!-- The Bootstrap Image Gallery lightbox, should be a child element of the document body --> | ||
| Line 232: | Line 184: | ||
<!-- The modal dialog, which will be used to wrap the lightbox content --> | <!-- The modal dialog, which will be used to wrap the lightbox content --> | ||
</div> | </div> | ||
| − | |||
| − | |||
<!-- Up HOME --> | <!-- Up HOME --> | ||
| − | + | <a href="#banner" class="gototop "><i class="fa fa-chevron-circle-up fa-3x"></i></a> | |
| − | + | ||
| − | + | ||
| − | + | ||
</body> | </body> | ||
Latest revision as of 00:01, 19 September 2015
What is the Game Over?
Allowing the end of the game
The game over concerns the end of one round in the Bio-Console game.
Indeed, you can not play indefinitely with the Bio-Console, and as every funny game something can happen to the hero!
Here, the end of the game is the death of the bacteria.
As you know, the goal of the game is simple: to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening.
The toxins will eliminate our hero that will lose his fluorescence or bioluminescence ... GAME OVER
Our game over is composed of two parts : Toxin-mediated delivery by photopolymerizable liposomes , clean-up of the microfluidic channel.
Photopolymerizable liposome
Toxin-mediated delivery
In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards.
For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm).
Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids.
In order to ensure functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”.
BactMan - Game Over
Because of a time issue, we did not synthesize the liposomes and therefore test the first part of our game over.
According to Yavlovich’s paper and if we follow carefully its liposome formation protocol, this system has a high probability to work efficiently.
Nevertheless, visible-light induced solute leakage from the liposome depends on the spectral properties of entrapped solutes. As we did not test the liposomes with the toxins, we cannot say if the leakage will be important or not. In any case, we do not really have to worry about it since we play upon red light.
References
Yavlovich A, Singh A, Blumenthal R, Puri A. A novel class of photo-triggerable liposomes containing DPPC:DC 8,9PC as vehicles for delivery of doxorubcin to cells. Biochim Biophys Acta - Biomembr. 2011;1808(1):117–26.
PROJECT
BactMan's Adventures
BIOBRICKS
Our parts of DNA
MICROFLUIDICS
Our challenge in achieving the Bio-Console