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==iGEM Prizes== | ==iGEM Prizes== | ||
===Best Integrated Human Practices=== | ===Best Integrated Human Practices=== | ||
| − | Our project is entirely dedicated to improving biosafety in iGEM and synthetic biology projects. When browsing previous years iGEM projects to understand what participating to the iGEM competition meant, | + | Our project is entirely dedicated to improving biosafety in iGEM and synthetic biology projects. When browsing previous years iGEM projects to understand what participating to the iGEM competition meant, a redundant observation highlighted the fact that biosafety was often a forgotten aspect in iGEM projects. Even when biosafety was taken into account, it seemed that it was integrated late in the conception of the project, after the choice of the chassis and the design of the system. In fact, we only found one project entirely focusing on biosafety (Paris Bettencourt 2012). This is when we decided to work on helping iGEM teams to better consider this aspect of their project. To determine whether biosafety was still undervalued by iGEM teams in 2015, we prepared a survey asking teams whether they had considered the possible effects of the release of their organism in the environment. Because we wanted to be useful to the community, we also asked which organism they chose as chassis. The results of this survey ([https://2015.igem.org/Team:Paris_Saclay/Description#survey Survey results]) showed us that (i)biosafety was still not a major concern for many iGEmers who answered our survey and that (ii) <i>Escherichia coli</i> was still the chassis of choice in iGEM projects. This incited us to consider several directions for our project. First we chose to focus on designing biosafety systems for <i>E. coli</i> chassis . Second, as some iGEMers seemed to consider that releasing <i>E. coli</i> in the environment was not a problem, as bacteria will die, we decided to investigate whether this was indeed the case ([https://2015.igem.org/Team:Paris_Saclay/Description#contamination contamination experiments]). Third, we thought that we should take into account physical containment as well as biological containment, as this would certainly be useful for devices aimed at being used in the environment (such as biosensors or bioremediation devices) ([https://2015.igem.org/Team:Paris_Saclay/Description#physical physical containment]). For the biological containment aspect of our project, we decided to concentrate on preventing the dissemination of GE <i>E. coli</i> in the environment, as the [https://2012.igem.org/Team:Paris_Bettencourt Paris Bettencourt] team 2012 already worked on preventing horizontal gene transfer with their Bware project. We chose to control the life or death of our bacteria using temperature, allowing them to survive in a defined range of temperatures ([https://2015.igem.org/Team:Paris_Saclay/Description#thermal thermal control]). Finally, we wanted the future iGEM teams to consider biosafety as an integral part of their projects, and not something to think about when writing the wiki or the safety form. We therefore wrote a Biosafety [https://static.igem.org/mediawiki/2015/0/05/Paris_Saclay-biosafety_guide_rouge_final.jpg guide], which aims at helping iGEM teams to take into account potential biosafety issues <b>at the beginning of the design of their system(s)</b>, rather than after the conception, and possibiliy the execution of their project. |
===Education and Public Engagement=== | ===Education and Public Engagement=== | ||
| − | We particularly focused on public engagement by building through the year an exhibition taking place during a science and art festival called CURIOSITas. This exhibition comprises a sculpture which is an original way to communicate with public about synthetic biology since it represents an “engineered” lemon tree which has two faces (“natural” vs “engineered”). It aims at making people think about what they will eat in 50 years and how could synthetic biology change their way of thinking about the living. Another interesting approach was the building of 3D Biobricks Legos which are used to make people basically and playfully understand how living organisms | + | We particularly focused on public engagement by building through the year an exhibition taking place during a science and art festival called [http://www.ladiagonale-paris-saclay.fr/curiositas/portfolio-items/ceci-nest-pas-un-citron/ CURIOSITas]. This festival is taking place at the same time as the giant Jamboree, but members of our team will be present at the festival. Our exhibition comprises a sculpture (realized with the help of a sculptor, Pascal Masi), which is an original way to communicate with public about synthetic biology since it represents an “engineered” lemon tree which has two faces (“natural” vs “engineered”). It aims at making people think about what they will eat in 50 years and how could synthetic biology change their way of thinking about the living. Another interesting approach was the building of 3D Biobricks Legos which are used to make people basically and playfully understand how living organisms can be engineered. The exhibition also contained classical posters aimed at popularizing synthetic biology and presenting the iGEM competition. A video presenting interviews of citizens about synthetic biology was also shown to get an idea about the knowledge and opinion of people on engineered living organisms. This small exhibition will be moving around different cities through the Parisian region during the Autumn. Furthermore, we plan to re-use it for the Science Festival, a national event taking place on October, 11th. |
<html><span class="anchor" id="curiositas"></span></html> | <html><span class="anchor" id="curiositas"></span></html> | ||
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====7th September - Meet up Leuven==== | ====7th September - Meet up Leuven==== | ||
[[File:Paris_Saclay-Human-Practice Leuven Meetup.gif|400px|left]] | [[File:Paris_Saclay-Human-Practice Leuven Meetup.gif|400px|left]] | ||
| − | The iGEM team of [https://2015.igem.org/Team:KU_Leuven KU_Leuven] invited us to join the synthetic biology and ethical [https://2015.igem.org/Team:KU_Leuven/Symposium Symposium] hosted at KU Campus of Leuven (Belgium). It was a great opportunity for us to present our 2015 project in front of an audience composed of scientists and other iGEM teams ([https://2015.igem.org/Team:TU_Eindhoven TU Eindhoven], [https://2015.igem.org/Team:Amsterdam Amsterdam] and [https://2015.igem.org/Team:KU_Leuven KU_Leuven]). We also have had the chance to attend conferences covering various aspects of the synthetic biology such as the functionality of a minimal synthetic organism, Cell-Free living, or even further by an | + | The iGEM team of [https://2015.igem.org/Team:KU_Leuven KU_Leuven] invited us to join the synthetic biology and ethical [https://2015.igem.org/Team:KU_Leuven/Symposium Symposium] hosted at KU Campus of Leuven (Belgium). It was a great opportunity for us to present our 2015 project in front of an audience composed of scientists and other iGEM teams ([https://2015.igem.org/Team:TU_Eindhoven TU Eindhoven], [https://2015.igem.org/Team:Amsterdam Amsterdam] and [https://2015.igem.org/Team:KU_Leuven KU_Leuven]). We also have had the chance to attend conferences covering various aspects of the synthetic biology such as the functionality of a minimal synthetic organism, Cell-Free living, or even further by an introduction to the entrepreneurial opportunities offered by synthetic biology. |
====21st August - Skype Aachen==== | ====21st August - Skype Aachen==== | ||
[[File:Paris_Saclay-Human-Practice Aachen.jpg|200px|right]] | [[File:Paris_Saclay-Human-Practice Aachen.jpg|200px|right]] | ||
| − | We had a chat with both the [https://2015.igem.org/Team:Aachen iGEM Aachen] and a Community German lab to lead a reflection on biosafety in experiments and more precisely on potential applications of our '''SafetE.coli''' project among iGEM community but also for external labs. The experience was an opportunity to present our | + | We had a chat with both the [https://2015.igem.org/Team:Aachen iGEM Aachen] and a Community German lab to lead a reflection on biosafety in experiments and more precisely on potential applications of our '''SafetE.coli''' project among iGEM community but also for external labs. The experience was an opportunity to present the principle of our physical containment system and to raise questions about its use limits. Moreover, the debate led us to get valuable informations about determining vital cellular concentration using a measurement capacity applicable in our [https://2015.igem.org/Team:Paris_Saclay/Description#physical physical containment system]. |
<html><span class="anchor" id="citizens"></span></html> | <html><span class="anchor" id="citizens"></span></html> | ||
| + | |||
==Contact with citizens== | ==Contact with citizens== | ||
====Paris-Montagne Association==== | ====Paris-Montagne Association==== | ||
[[File:Paris_Saclay-Human-Practice ParisMontagne .gif|400px|right]] | [[File:Paris_Saclay-Human-Practice ParisMontagne .gif|400px|right]] | ||
| − | We collaborated with the Paris-Montagne Association to organize a scientific meeting. Hosted | + | We collaborated with the Paris-Montagne Association to organize a scientific meeting. Hosted at the campus of the University Paris-Saclay, the event intended to connect researchers and students coming from disadvantaged backgrounds to introduce them to the practice of research and give them a taste for scientific studies. After explaining fundamentals and challenges of synthetic biology, members of the 2015 iGEM Paris-Saclay team held a discussion with the group of High school students and helped them to think about a future iGEM project. Some ideas such as designing a fluorescent human body appears to be very popular among the group of young students! In order to transpose theory into practice, we offered the young scientists a private visit into the lab ([http://www.i2bc.paris-saclay.fr Institute of Integrative Biology of the Cell]). They had an overview of the necessary tools and techniques commonly used to manipulate genome. |
====IT Magazine==== | ====IT Magazine==== | ||
[[File:Paris_Saclay-Human-Practice IT.jpg|200px|left]] | [[File:Paris_Saclay-Human-Practice IT.jpg|200px|left]] | ||
| − | IT is a web magazine publishing articles covering subjects about industries and technologies. We corresponded with one of their journalists to explain the iGEM competition, the main concepts of synthetic biology and its numerous | + | IT is a web magazine publishing articles covering subjects about industries and technologies. We corresponded with one of their journalists to explain the iGEM competition, the main concepts of synthetic biology and its numerous applications. We also presented the concept and objectives of our '''SafetE.coli''' project with a simplified view so it can reach the general public. Therefore an [http://www.industrie-techno.com/igem-2015-l-equipe-paris-saclay-veut-eviter-la-dissemination-des-bacteries-genetiquement-modifiees.39207 article dedicated to the project of the 2015 iGEM Paris-Saclay team was published] on the 28<sup>th</sup> of August 2015. |
====La fête de la science (The Science Party)==== | ====La fête de la science (The Science Party)==== | ||
[[File:Paris_Saclay-Human-Practice Fiesta 2.JPG|300px|right]] | [[File:Paris_Saclay-Human-Practice Fiesta 2.JPG|300px|right]] | ||
| − | [http://www.fetedelascience.fr “La fête de la science”] is | + | [http://www.fetedelascience.fr “La fête de la science”] is a national Science Festival undertaken by the Ministry of National Research. Scientific events are held all over the country to promote sciences and to interact with people from different backgrounds. The iGEM Paris-Saclay team attends every year events in the campus that hosts students, personal, academic and general public in its laboratories in biology, chemistry, computer science, mathematics, physics and geology. Our team is planning activities for this year festival that will take place on October 11<sup>th</sup>. The main purpose is to induce debate and promote synthetic biology by exhibiting popularization posters made by our team and potentially meet students interested in joining the iGEM Paris-Saclay 2016 team. |
<html><span class="anchor" id="collaborations"></span></html> | <html><span class="anchor" id="collaborations"></span></html> | ||
| Line 85: | Line 86: | ||
====iGEM 2015 teams Biosafety Survey==== | ====iGEM 2015 teams Biosafety Survey==== | ||
<html><article style="float:right; margin-left:20px;"></html>{{Team:Paris_Saclay/survey_badge|width=250px}}<html></article></html> | <html><article style="float:right; margin-left:20px;"></html>{{Team:Paris_Saclay/survey_badge|width=250px}}<html></article></html> | ||
| − | Our '''SafetE.coli''' project implied to know more about the chassis used among iGEM teams.([https://docs.google.com/forms/d/1VSC9fclmluGj695NOOeS8QLasHjYptxQQtQYiTynio8/viewform?c=0&w=1 Survey]).Besides, we needed to know more about safety measures undertaken by other teams. A biosafety survey had been made by our team and sent to all 2015 teams asking them to answer few questions about their project. A [http://twitter.com/iGEMParisSaclay/status/639350658852892672 contributing Badge] has been sent to collaborative teams. Many of them did publish it on their wiki. The results from the survey | + | Our '''SafetE.coli''' project implied to know more about the chassis used among iGEM teams.([https://docs.google.com/forms/d/1VSC9fclmluGj695NOOeS8QLasHjYptxQQtQYiTynio8/viewform?c=0&w=1 Survey]).Besides, we needed to know more about safety measures undertaken by other teams. A biosafety survey had been made by our team and sent to all 2015 teams asking them to answer few questions about their project. A [http://twitter.com/iGEMParisSaclay/status/639350658852892672 contributing Badge] has been sent to collaborative teams. Many of them did publish it on their wiki. The results from the survey are considered as background and testifies of the frequency of the chassis used in the '''SafetE.coli''' project. Additionally, it shows the amount of teams that are really considering a system to avoid dissemination of their engineered organism in the environment and its limits. |
| − | + | Results of the survey can be accessed here:[https://2015.igem.org/Team:Paris_Saclay/Description#survey Survey results] | |
====Draw my Biosafety==== | ====Draw my Biosafety==== | ||
| Line 102: | Line 103: | ||
====iGEM Rhizi==== | ====iGEM Rhizi==== | ||
[[File:Paris_Saclay-Human-Practice Rhizi.jpg|300px|right]] | [[File:Paris_Saclay-Human-Practice Rhizi.jpg|300px|right]] | ||
| − | Our team enriched the [https://2015.igem.org/Team:Paris_Bettencourt/Practices/Rhizi graphic database], initiated by [https://2015.igem.org/Team:Paris_Bettencourt iGEM Paris-Bettencourt], by sharing key information of our '''SafetE.coli''' project. Thus completing the platform with keywords relating to our project make us visible by other iGEM teams. It is a fast and interactive way to find out subjects on which are working other teams. | + | Our team enriched the [https://2015.igem.org/Team:Paris_Bettencourt/Practices/Rhizi graphic database], initiated by [https://2015.igem.org/Team:Paris_Bettencourt iGEM Paris-Bettencourt], by sharing key information of our '''SafetE.coli''' project. Thus completing the platform with keywords relating to our project make us visible by other iGEM teams. It is a fast and interactive way to find out subjects on which are working other teams. Besides the fact that the platform was available lately in 2015 Competition, Rhizi is promising for future teams. This could certainly become a fast way to find possible collaborations among the iGEM community. |
====Newsletter==== | ====Newsletter==== | ||
Participating in the festival combining art and science is a tradition for the team iGEM Paris -Saclay .This year we decided to continue the uncompleted project of team iGEM Paris-Saclay 2014. We fashioned several posters intended to popularize synthetic biology. Moreover we realized a piece of art in collaboration with a sculptor. This work will be exposed in several exhibitions through the Parisian region.Read more
One of the main exciting aspects about iGEM competition lies in the multitude of opportunities to meet other people around the world who share this same passion for sciences. These Meet-ups, in addition to be an opportunity to have fun, represent a framework to share experiences and discussions about our respective projects.Read more
Because making people getting interested in sciences, synthetic biology and more precisely in our project, iGEM Paris-Saclay team made of Popularization one of its priority. We conducted various activities to get to know people interested in sciences but not necessarily coming from a scientific field. We designed different devices to facilitate basic comprehension of synthetic biology and of the SafetE.coli iGEM project. Read more
The incessant growing number of teams joining iGEM competition increases possibilities of collaborations. Therefore, besides the fact that we participated to other team requests, we drove activities to help and interact with other 2015 iGEM teams. Read more
Our project is entirely dedicated to improving biosafety in iGEM and synthetic biology projects. When browsing previous years iGEM projects to understand what participating to the iGEM competition meant, a redundant observation highlighted the fact that biosafety was often a forgotten aspect in iGEM projects. Even when biosafety was taken into account, it seemed that it was integrated late in the conception of the project, after the choice of the chassis and the design of the system. In fact, we only found one project entirely focusing on biosafety (Paris Bettencourt 2012). This is when we decided to work on helping iGEM teams to better consider this aspect of their project. To determine whether biosafety was still undervalued by iGEM teams in 2015, we prepared a survey asking teams whether they had considered the possible effects of the release of their organism in the environment. Because we wanted to be useful to the community, we also asked which organism they chose as chassis. The results of this survey (Survey results) showed us that (i)biosafety was still not a major concern for many iGEmers who answered our survey and that (ii) Escherichia coli was still the chassis of choice in iGEM projects. This incited us to consider several directions for our project. First we chose to focus on designing biosafety systems for E. coli chassis . Second, as some iGEMers seemed to consider that releasing E. coli in the environment was not a problem, as bacteria will die, we decided to investigate whether this was indeed the case (contamination experiments). Third, we thought that we should take into account physical containment as well as biological containment, as this would certainly be useful for devices aimed at being used in the environment (such as biosensors or bioremediation devices) (physical containment). For the biological containment aspect of our project, we decided to concentrate on preventing the dissemination of GE E. coli in the environment, as the Paris Bettencourt team 2012 already worked on preventing horizontal gene transfer with their Bware project. We chose to control the life or death of our bacteria using temperature, allowing them to survive in a defined range of temperatures (thermal control). Finally, we wanted the future iGEM teams to consider biosafety as an integral part of their projects, and not something to think about when writing the wiki or the safety form. We therefore wrote a Biosafety guide, which aims at helping iGEM teams to take into account potential biosafety issues at the beginning of the design of their system(s), rather than after the conception, and possibiliy the execution of their project.
We particularly focused on public engagement by building through the year an exhibition taking place during a science and art festival called [http://www.ladiagonale-paris-saclay.fr/curiositas/portfolio-items/ceci-nest-pas-un-citron/ CURIOSITas]. This festival is taking place at the same time as the giant Jamboree, but members of our team will be present at the festival. Our exhibition comprises a sculpture (realized with the help of a sculptor, Pascal Masi), which is an original way to communicate with public about synthetic biology since it represents an “engineered” lemon tree which has two faces (“natural” vs “engineered”). It aims at making people think about what they will eat in 50 years and how could synthetic biology change their way of thinking about the living. Another interesting approach was the building of 3D Biobricks Legos which are used to make people basically and playfully understand how living organisms can be engineered. The exhibition also contained classical posters aimed at popularizing synthetic biology and presenting the iGEM competition. A video presenting interviews of citizens about synthetic biology was also shown to get an idea about the knowledge and opinion of people on engineered living organisms. This small exhibition will be moving around different cities through the Parisian region during the Autumn. Furthermore, we plan to re-use it for the Science Festival, a national event taking place on October, 11th.
CURIOSITas is a rotary festival born from the meeting between scientists and artists and is held at Orsay but also transported into different cities of the Parisian region from September 24th to October 24th. iGEM Paris-Saclay team is presenting an exhibition called « This is not a lemon » which is related to last year iGEM Paris-Saclay 2014 project. We decided to continue the story by creating a sculpture made by the 2015 iGEM Paris-Saclay team in collaboration with the sculptor [http://pascal-masi.com/ Pascal Masi]. The project of the iGEM Paris-Saclay team 2014 aims to create reflexion around the following questions:
Moreover the iGEM exhibition presents synthetic biology and invites public to explore the new frontiers of living. We have planned an interactive installation made by seven posters designed by the 2015 iGEM Paris-Saclay team: describing history, principal and functional applications of synthetic biology.
Also in collaboration with [http://fablabdigiscope.wordpress.com/ Fablab Digiscop], we printed a set of 3D Biobricks Legos composed of a promotor, a coding sequence and a terminator.
To complete our eye-catching exhibition, a video will be available. These short movies present key moments extracted from several interviews made by our team in Paris. The video first shows people’s reflexion about what synthetic biology could be used for and then what ethical issues they think it raises. This is a good way to simplify and integrate concepts of biology engineering and make it accessible to the targeted public of this festival: families, children, youths, students, scientists and artists and all those who want to discover this original project that push the boundaries of art and science.
Attending the Parisian meetup hosted by iGEM Pasteur team, was an opportunity to interact with members of the Parisian teams. The presentations on the different iGEM 2015 projects, although not yet fully accomplished at that time, were sufficient to understand the origin, reflexion, and main challenges of each project conducted by the involved teams (Ionis, Evry, Paris-Bettencourt, and Paris-Pasteur). Additionally, since the meet-up took place at Pasteur Institute, we visited the Pasteur Museum and the house of Louis Pasteur which brought us an overview about his main contributions to biology. Finally we had a funny extra collaboration with Ionis iGEM team: they filmed a short video in which we presented the main lines around our SafetE.coli project.
Our team has made experience sharing and interaction with other iGEM teams one of its priorities. Thus we have covered hundred of miles to the city of Bordeaux by joining a scavenger hunt, based on questions about biology and iGEM projects, with team of Bordeaux, KU_Leuven, EPLF-Lausanne, Paris-Pasteur, Aix-Marseille and Toulouse. The meet up gave us a unique opportunity to present our respective projects in a popularize fashion so it could be accessible to an audience unfamiliar with the principles of synthetic biology in the premises of Science Museum in Bordeaux ([http://www.cap-sciences.net Cap Sciences]). A theatre expert gave us a constructive feedbacks and valuable advices about our presentation. Besides popularization activities, we participated to a discussion about the project of the iGEM Pasteur team and answered a survey helping them to consider impact of the throw of plastic waste in the oceans. Finally our team conducted a debate about biosafety and how it is important to consider it in each iGEM project.
The iGEM team of KU_Leuven invited us to join the synthetic biology and ethical Symposium hosted at KU Campus of Leuven (Belgium). It was a great opportunity for us to present our 2015 project in front of an audience composed of scientists and other iGEM teams (TU Eindhoven, Amsterdam and KU_Leuven). We also have had the chance to attend conferences covering various aspects of the synthetic biology such as the functionality of a minimal synthetic organism, Cell-Free living, or even further by an introduction to the entrepreneurial opportunities offered by synthetic biology.
We had a chat with both the iGEM Aachen and a Community German lab to lead a reflection on biosafety in experiments and more precisely on potential applications of our SafetE.coli project among iGEM community but also for external labs. The experience was an opportunity to present the principle of our physical containment system and to raise questions about its use limits. Moreover, the debate led us to get valuable informations about determining vital cellular concentration using a measurement capacity applicable in our physical containment system.
We collaborated with the Paris-Montagne Association to organize a scientific meeting. Hosted at the campus of the University Paris-Saclay, the event intended to connect researchers and students coming from disadvantaged backgrounds to introduce them to the practice of research and give them a taste for scientific studies. After explaining fundamentals and challenges of synthetic biology, members of the 2015 iGEM Paris-Saclay team held a discussion with the group of High school students and helped them to think about a future iGEM project. Some ideas such as designing a fluorescent human body appears to be very popular among the group of young students! In order to transpose theory into practice, we offered the young scientists a private visit into the lab ([http://www.i2bc.paris-saclay.fr Institute of Integrative Biology of the Cell]). They had an overview of the necessary tools and techniques commonly used to manipulate genome.
IT is a web magazine publishing articles covering subjects about industries and technologies. We corresponded with one of their journalists to explain the iGEM competition, the main concepts of synthetic biology and its numerous applications. We also presented the concept and objectives of our SafetE.coli project with a simplified view so it can reach the general public. Therefore an [http://www.industrie-techno.com/igem-2015-l-equipe-paris-saclay-veut-eviter-la-dissemination-des-bacteries-genetiquement-modifiees.39207 article dedicated to the project of the 2015 iGEM Paris-Saclay team was published] on the 28th of August 2015.
[http://www.fetedelascience.fr “La fête de la science”] is a national Science Festival undertaken by the Ministry of National Research. Scientific events are held all over the country to promote sciences and to interact with people from different backgrounds. The iGEM Paris-Saclay team attends every year events in the campus that hosts students, personal, academic and general public in its laboratories in biology, chemistry, computer science, mathematics, physics and geology. Our team is planning activities for this year festival that will take place on October 11th. The main purpose is to induce debate and promote synthetic biology by exhibiting popularization posters made by our team and potentially meet students interested in joining the iGEM Paris-Saclay 2016 team.
Seven iGEM teams attended the Bordeaux Meetup. We seized this opportunity to spark reflections concerning every iGEM projects. Therefore we encouraged present teams to think about a safety mechanism suitable for their project. We then helped them to optimize it in order to prevent dissemination of their genetically engineered organism. To make it a funny and traceable experience we asked each team to draw the mechanism developed.
To generalize concern of biosafety among iGEM projects and make it accessible we developed a guide.This document aims to simplify the process in order to conceptualize a biosafety point of view. Moreover this guide rises major questions that a team should consider when starting a biosafety consideration about a project.
Members of our team answered the questions and advised some of the founder members of iGEM Paris Pasteur on how to run an iGEM team. iGEM Pasteur is participating for the first time this year to iGEM competition.
Our team enriched the graphic database, initiated by iGEM Paris-Bettencourt, by sharing key information of our SafetE.coli project. Thus completing the platform with keywords relating to our project make us visible by other iGEM teams. It is a fast and interactive way to find out subjects on which are working other teams. Besides the fact that the platform was available lately in 2015 Competition, Rhizi is promising for future teams. This could certainly become a fast way to find possible collaborations among the iGEM community.
We have completed the following surveys from various teams.
Copyright © 2015 iGEM Paris Saclay. 