Difference between revisions of "Team:Aix-Marseille/Notebook2"

 
(13 intermediate revisions by 2 users not shown)
Line 4: Line 4:
 
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
 
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
         <link rel="stylesheet" href="https://2015.igem.org/Team:Aix-Marseille/css?action=raw&amp;ctype=text/css" type="text/css">
+
         <link rel="stylesheet" href="https://2015.igem.org/Team:NRP-UEA-Norwich/bootstrapcss?action=raw&amp;ctype=text/css" type="text/css">
<link rel="stylesheet" href="https://2015.igem.org/Team:Aix-Marseille/cssmobile?action=raw&amp;ctype=text/css" type="text/css">
+
<link rel="stylesheet" href="https://2015.igem.org/Team:NRP-UEA-Norwich/mobilecss?action=raw&amp;ctype=text/css" type="text/css">
         <script type="text/javascript" src="https://2015.igem.org/Team:Aix-Marseille/java?action=raw&ctype=text/javascript"></script>
+
         <script type="text/javascript" src="https://2015.igem.org/Team:NRP-UEA-Norwich/bootstrap.js?action=raw&ctype=text/javascript"></script>
  
 
         <!--[if lt IE 7]>
 
         <!--[if lt IE 7]>
Line 33: Line 33:
 
color: #FFFFFF !important;
 
color: #FFFFFF !important;
 
} .bg-1 {
 
} .bg-1 {
background: url(http://i.imgur.com/FrsJhjG.jpg);background-repeat:no-repeat;
+
background: url(https://static.igem.org/mediawiki/2015/f/f3/FrsJhjG.jpg);background-repeat:no-repeat;
 
         background-size:cover;
 
         background-size:cover;
 
min-height: 600px;
 
min-height: 600px;
Line 47: Line 47:
 
               new WOW().init();
 
               new WOW().init();
 
               </script>
 
               </script>
<meta name="viewport" content="width=device-width, initial-scale=1">
 
 
 
  <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.3/jquery.min.js"></script>
 
 
  <script src="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/js/bootstrap.min.js"></script>
 
 
</head>
 
</head>
 
<body>
 
<body>
Line 70: Line 64:
  
 
                         </button>
 
                         </button>
                         <a href="/Team:Aix Marseille" class="navbar-brand hidden-lg hidden-md hidden-sm" ><http://i.imgur.com/oHSATfM.png" class="img-responsive" width="80" height="80"></a>
+
                         <a href="/Team:Aix Marseille" class="navbar-brand hidden-lg hidden-md hidden-sm" ><https://static.igem.org/mediawiki/2015/6/6e/OHSATfM.png" class="img-responsive" width="80" height="80"></a>
 
      
 
      
 
                     </div>
 
                     </div>
Line 77: Line 71:
 
                             <div class="collapse navbar-collapse  " id="bs-example-navbar-collapse-1">
 
                             <div class="collapse navbar-collapse  " id="bs-example-navbar-collapse-1">
 
                                 <ul class="nav navbar-nav  " id="nav">
 
                                 <ul class="nav navbar-nav  " id="nav">
                                 <li><a href="/Team:Aix-Marseille"  class="logo"><img src="http://i.imgur.com/oHSATfM.png" class="img-responsive" width="85" height="85"></a></li>
+
                                 <li><a href="/Team:Aix-Marseille"  class="logo"><img src="https://static.igem.org/mediawiki/2015/6/6e/OHSATfM.png" class="img-responsive" width="85" height="85"></a></li>
 
                                     <li  ><a href="/Team:Aix-Marseille">Home</a></li>
 
                                     <li  ><a href="/Team:Aix-Marseille">Home</a></li>
 
                                       <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Project</a>
 
                                       <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Project</a>
 
                                       <ul class="dropdown-menu">
 
                                       <ul class="dropdown-menu">
                                         <li ><a href="/Team:Aix-Marseille/Description">Overview</a></li>
+
                                         <li ><a href="/Team:Aix-Marseille/Project/Description">Overview</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Design">Project design</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Design">Project design</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Parts">Parts</a></li>                                   
 
                                         <li ><a href="/Team:Aix-Marseille/Parts">Parts</a></li>                                   
Line 105: Line 99:
 
                                         <li ><a href="/Team:Aix-Marseille/Notebook2">Calendar</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Notebook2">Calendar</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Measurement">Results</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Measurement">Results</a></li>
                                         <li ><a href="/Team:Aix-Marseille/Protocols2">Protocols2</a></li>
+
                                         <li ><a href="/Team:Aix-Marseille/Protocols2">Protocols</a></li>
 
                                         <li ><a href="/Team:Aix-Marseille/Sequencing/data">Sequencing data</a></li>                                   
 
                                         <li ><a href="/Team:Aix-Marseille/Sequencing/data">Sequencing data</a></li>                                   
 
                                       </ul>
 
                                       </ul>
Line 114: Line 108:
 
                                       </ul>     
 
                                       </ul>     
 
                                     </li>
 
                                     </li>
                        
+
                       <li class="dropdown " id="dropdown"><a href="/Team:Aix-Marseille/Achievement" class="dropdown-toggle"  data-toggle="dropdown">Achievement</a></li>
 
                                   </li>
 
                                   </li>
  
Line 131: Line 125:
  
 
     </header>
 
     </header>
 
 
 
 
  
  
Line 224: Line 214:
 
<li>BBa_J23103 + I13504 + pSB1K3</li>
 
<li>BBa_J23103 + I13504 + pSB1K3</li>
 
<li>BBa_K525998 + I13401 + pSB1K3</li>
 
<li>BBa_K525998 + I13401 + pSB1K3</li>
 +
</ul>
 
<p>Transformation of ligation products</p>
 
<p>Transformation of ligation products</p>
 
<p>PCR on some M1 clones.</p>
 
<p>PCR on some M1 clones.</p>
Line 243: Line 234:
 
<li>BBa_K525998 + I13401 + pSB1K3</li>
 
<li>BBa_K525998 + I13401 + pSB1K3</li>
 
<li>And PCR  M1 relaunch </li>
 
<li>And PCR  M1 relaunch </li>
 +
</ul>
  
 
<p>Starters launched of positive clones</p>
 
<p>Starters launched of positive clones</p>
Line 302: Line 294:
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo11">July 15</button>
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo11">July 15</button>
 
   <div id="demo11" class="collapse">
 
   <div id="demo11" class="collapse">
Transformation of J23103 + I13504 + pSB1K3 into E.coli (DH5-Alpha)
+
Transformation of J23103 + I13504 + pSB1K3 into <i>E.coli</i> (DH5-Alpha)
 
</div>
 
</div>
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo12">July 16</button>
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo12">July 16</button>
Line 326: Line 318:
 
<p>Ligation in pSB1C3 </p>
 
<p>Ligation in pSB1C3 </p>
  
<p>Transformation into E.coli (DH5-Alpha) of:</p>
+
<p>Transformation into <i>E.coli</i> (DH5-Alpha) of:</p>
 
<ul>
 
<ul>
 
<li>J23100 + I13504 + pSB1C3</li>
 
<li>J23100 + I13504 + pSB1C3</li>
Line 376: Line 368:
 
<li>J23103 + I13504 + pSB1C3</li>
 
<li>J23103 + I13504 + pSB1C3</li>
 
<li>K525998 + I13401 + pSB1C3</li>
 
<li>K525998 + I13401 + pSB1C3</li>
 +
</ul>
  
 
<p>Digestion E/P and electrophoresis to see if the insertion is good</p>
 
<p>Digestion E/P and electrophoresis to see if the insertion is good</p>
Line 417: Line 410:
 
</ul>
 
</ul>
 
<p>Transformation into <i>E.coli</i> (TG1)</p>
 
<p>Transformation into <i>E.coli</i> (TG1)</p>
 +
<ul>
 
<li>BBa_J23118 + I13504 + pSB1C3</li>
 
<li>BBa_J23118 + I13504 + pSB1C3</li>
 
<li>BBa_K823013 + I13504 + pSB1C3</li>
 
<li>BBa_K823013 + I13504 + pSB1C3</li>
Line 611: Line 605:
 
<p>Determination of the medium that will be use => LB or PBS1X</p>
 
<p>Determination of the medium that will be use => LB or PBS1X</p>
 
   </div>
 
   </div>
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo42">August 27</button>
+
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo43">August 27</button>
 
   <div id="demo43" class="collapse">
 
   <div id="demo43" class="collapse">
 
<p>Ligation K823013 + I13504 + pSB1C3</p>
 
<p>Ligation K823013 + I13504 + pSB1C3</p>
Line 626: Line 620:
 
   </div>
 
   </div>
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo45">August 31</button>
 
   <button type="button" class="btn btn-info" data-toggle="collapse" style="width: 110px;" data-target="#demo45">August 31</button>
   <div id="demo46" class="collapse">
+
   <div id="demo45" class="collapse">
 
Digestion E/P of K823013 + I13504 + pSB1C3
 
Digestion E/P of K823013 + I13504 + pSB1C3
 
   </div>
 
   </div>
Line 649: Line 643:
 
<h1 class="blue"><div align ="center">MORE INFORMATION ON FACEBOOK !</div></h1>
 
<h1 class="blue"><div align ="center">MORE INFORMATION ON FACEBOOK !</div></h1>
 
                     <ul class="list-inline space80 icon">
 
                     <ul class="list-inline space80 icon">
                         <li><div align ="center"><a href="https://www.facebook.com/iGEM.AMU?fref=ts"><img src="http://i.imgur.com/jvnAC0t.png" class="img-grey mautomargin"></a></li></div>
+
                         <li><div align ="center"><a href="https://www.facebook.com/iGEM.AMU?fref=ts"><img src="https://static.igem.org/mediawiki/2015/e/ed/FacebookAMU.png" width="200" height="200" class="img-grey mautomargin"></a></li></div>
 
                     </ul>
 
                     </ul>
  
Line 667: Line 661:
 
                 <div class="col-md-12 text-center">
 
                 <div class="col-md-12 text-center">
 
                     <h2 class="gray">SPONSORS</h2>
 
                     <h2 class="gray">SPONSORS</h2>
                    <ul class="list-inline space30 icon">
+
<ul class="list-inline space30 icon">
                        <li><a href="http://polytech.univ-amu.fr/"><img src="http://i.imgur.com/iLFZ36R.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html">
                        <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social"><img src="http://i.imgur.com/KlP5G8h.png" class="img-grey mautomargin" width="200" height="150"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/8/87/Corninglogo.jpg" width="200" align="center"
                        <li><a href="http://www.univ-amu.fr/"><img src="http://i.imgur.com/EcfF9Kw.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin"></a></li>
                        <li><a href="http://www.cnrs.fr/"><img src="http://i.imgur.com/IaWMSIr.jpg" width="200" height="150" class="img-grey mautomargin"></a></li>
+
  <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social">
                        <li><a href="http://www3.gehealthcare.fr/"><img src="http://i.imgur.com/ryVz5vv.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/1/18/KlP5G8h.jpg"  
                        <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html"><img src="http://i.imgur.com/ycgjpR7.jpg" width="225" height="100" class="img-grey mautomargin"></a></li>
+
    class="img-grey mautomargin" width="200" align="center"></a></li>
                        <li><a href="http://www.grandluminy.com/"><img src="http://i.imgur.com/GK5IMdz.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
  <li><a href="http://www.cnrs.fr/">
                        <li><a href="https://www.eppendorf.com/FR-fr/"><img src="http://i.imgur.com/jUjj2YD.jpg" width="250" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/8/8e/CNRSlogo.jpg"
                        <li><a href="http://www.dutscher.com/frontoffice/home;jsessionid=4164FFDE7B048361D89406E266542A13"><img src="http://i.imgur.com/wjZ6U1A.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    width="200" class="img-grey mautomargin" align="center"></a></li>
<li><a href="http://www.mn-net.com/"><img src="http://i.imgur.com/apuJ5in.jpg" width="200" height="100" class="img-grey mautomargin"></a></li>
+
  <li><a href="https://www.qiagen.com/fr/">
<li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services"><img src="http://i.imgur.com/5tokNzS.png" width="200" height="100" class="img-grey mautomargin"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/f/f1/Qialogo.jpg" width="200" align="center"
<li><a href="https://www.qiagen.com/fr/"><img src="http://i.imgur.com/4jXkcLB.jpg" width="200" height="100"class="img-grey mautomargin"></a></li>
+
    class="img-grey mautomargin"></a></li>
<li><a href="https://www.sigmaaldrich.com/france.html"><img src="http://i.imgur.com/PUNXaNj.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="http://www.univ-amu.fr/">
<li><a href="http://www.starlab.de/int/?l=5"><img src="http://i.imgur.com/SsgB8IC.jpg" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"  
<li><a href="http://fr.ambafrance-us.org/"><img src="http://i.imgur.com/m3ieC2R.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin" width="200" align="center" ></a></li>
<li><a href="https://www.neb.com/"><img src="http://i.imgur.com/ll3uagr.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
  <li><a href="http://polytech.univ-amu.fr/">
<li><a href="http://sciences.univ-amu.fr/"><img src="http://i.imgur.com/FtFgEFj.png" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"  
<li><a href="https://eu.idtdna.com/site"><img src="http://i.imgur.com/ocgTw7q.jpg" class="img-grey mautomargin" width="200" height="100"></a></li>
+
    class="img-grey mautomargin" width="200" align="center"></a></li>
 
+
  <li><a href="http://www.grandluminy.com/">
 
+
    <img src="https://static.igem.org/mediawiki/2015/6/66/GandLlogo.jpg" width="200" align="center"
 
+
    class="img-grey mautomargin"></a></li>
                    </ul>
+
  <li><a href="https://www.eppendorf.com/FR-fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/db/Eppendorflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.dutscher.com/frontoffice/home">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/10/Deutcherflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.mn-net.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/1b/MNlogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/3d/5tokNzS.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www3.gehealthcare.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/bd/GEHlogo.jpg" width="200"  
 +
    class="img-grey mautomargin" align="center" ></a></li>
 +
  <li><a href="https://www.sigmaaldrich.com/france.html">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/41/PUNXaNj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://www.starlab.de/int/?l=5">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/13/StarLablogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="http://fr.ambafrance-us.org/">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/2b/M3ieC2R.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="https://www.neb.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Ll3uagr.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://sciences.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/4a/FtFgEFj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="https://eu.idtdna.com/site">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/d9/IDTlogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
</ul>
  
 
                    
 
                    
 
                 </div>
 
                 </div>
  
             </div>  
+
             </div>
 
               <div class="space20"></div>
 
               <div class="space20"></div>
 
         </div>
 
         </div>

Latest revision as of 00:07, 19 September 2015

Chew fight

CALENDAR

MORE INFORMATION ON FACEBOOK !

Useful Links

Contact Us


  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition. See you soon in Boston !