Difference between revisions of "Team:Lethbridge/Measurement"

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{{Lethbridge}}
 
 
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        <title>iGEM</title>
  
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<h2> Measurement</h2>
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<h4>Note</h4>
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        <script type="text/javascript" src="https://2015.igem.org/Team:Lethbridge/jQuery2-1-3_js?action=raw&ctype=text/javascript"></script>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Measurement Approach award</a>, you must fill out this page.</p>
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<p>There are a lot of exciting Parts in the Registry, but many Parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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        <div id="wrapper">
  
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            <div id="menu_bar">
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                <ul>
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                    <li><a href="https://2015.igem.org/">iGEM</a></li>
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                    <li><a href="https://2015.igem.org/Team:Lethbridge">RNAiCare</a></li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Project">Project</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Description">Description</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Design">Design</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Results">Results</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Production">Production</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Judging">Judging</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Parts">Parts</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Basic_Part">Basic Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Composite_Part">Composite Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Measurement">Measurement</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Practices">Practices</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Risks">Risks</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Stakeholders">Stakeholders</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Current">Current Problems</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Notebook">Notebook</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_July">July</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_August">August</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_September">September</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Team">Team</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Team_Members">Members</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Attributions">Attributions</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
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                        </ul>
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                    </li>
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<h4>Inspiration</h4>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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                    <h1>Measurement</h1>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus">2014 Valencia Biocampus</a></li>
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                    <p>Our team decided to take part in this year’s interlab measurement study, measuring fluorescence of GFP in devices 1, 2 and 3. The GFP sequence was cleaved from I13504, using restriction enzymes Xba1 and Pst1, and ligated into pSB1C3 plasmid containing the following parts J23101, J23106 and J23117. Sequencing and restriction mapping was used to confirm proper insertion of the GFP sequence. Devices were transformed into DH5α cells. DH5α cells containing the devices and controls were normalized, lysed and GFP measurements were taken using a fluorometer.</p>
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                    <img src="https://static.igem.org/mediawiki/2015/1/14/Uleth_Interlab1.png" height="30%" width="30%">
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                    <img src="https://static.igem.org/mediawiki/2015/6/6b/Uleth_Interlab2.png" height="30%" width="30%">
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                    <img src="https://static.igem.org/mediawiki/2015/a/a3/Uleth_Interlab3.png" height="30%" width="30%">
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                    <p>The measurement of iGEM Interlab study devices yielded some unexpected results. Of the three devices, only Device 2 produced measurably different fluorescence levels than both the empty plasmid and no plasmid negative controls (Fig. 1). This questionable fluorescence data could be attributed to incorrect assembly of devices from the iGEM kit plates, as successful construct assembly was screened using restriction mapping only. In the future, it would be beneficial for iGEM headquarters to provide completed DNA constructs for large collaborative studies.</p>
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<img src="https://static.igem.org/mediawiki/2015/9/9c/Uleth15_InterlabData1.png" height="40%" width="40%">
 
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<p>Figure 1. Cell lysate fluorescence of iGEM Interlab study devices measured using fluorescence spectrophotometry (n = 3, mean ą s.d.)</p>
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Latest revision as of 00:13, 19 September 2015

iGEM

Measurement

Our team decided to take part in this year’s interlab measurement study, measuring fluorescence of GFP in devices 1, 2 and 3. The GFP sequence was cleaved from I13504, using restriction enzymes Xba1 and Pst1, and ligated into pSB1C3 plasmid containing the following parts J23101, J23106 and J23117. Sequencing and restriction mapping was used to confirm proper insertion of the GFP sequence. Devices were transformed into DH5α cells. DH5α cells containing the devices and controls were normalized, lysed and GFP measurements were taken using a fluorometer.

The measurement of iGEM Interlab study devices yielded some unexpected results. Of the three devices, only Device 2 produced measurably different fluorescence levels than both the empty plasmid and no plasmid negative controls (Fig. 1). This questionable fluorescence data could be attributed to incorrect assembly of devices from the iGEM kit plates, as successful construct assembly was screened using restriction mapping only. In the future, it would be beneficial for iGEM headquarters to provide completed DNA constructs for large collaborative studies.

Figure 1. Cell lysate fluorescence of iGEM Interlab study devices measured using fluorescence spectrophotometry (n = 3, mean ą s.d.)