Difference between revisions of "Team:WLC-Milwaukee/Description"

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<body style="background-image:url('https://static.igem.org/mediawiki/2015/b/bc/ProjectHeader.JPG');background-repeat:no-repeat;background-size:100%">
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<h2> Project Description </h2>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h4>Advice on writing your Project Description</h4>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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</p>
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<h4>References</h4>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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    <p>Description</p>
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<p>Expression of Salmonella and Vibrio cholerae TolC protein – A western blot was performed using α-TolC specific against Escherichia coli antibodies. These antibodies also recognize the TolC proteins from Salmonella  and Vibrio cholerae. Lane 1 contains the protein ladder (NEB Prestained protein ladder, broad range, NEB #7702) which demonstrates the sizes of the TolC proteins displayed in the western blot are correct  according to the predicted molecular weights for each. The next two lanes are  wild-type E. coli  (BW25113) with the chromosomal tolC gene (Lane 2) or without (Lane 3). The following three lanes are the E. coli strains containing the  tolC deletion and the pUC57 plasmid with the arabinose promoter alone (Lane 4), the arabinose promoter followed by the  Salmonella tolC gene (Lane 5), or the arabinose promoter followed by the Vibrio cholerae tolC gene (Lane 6). No arabinose was added to the cultures used for the samples in Lanes 4, 5, and 6. Lanes  7, 8, and 9 were the same strains as the previous three lanes except that 0.1% arabinose was added to each culture. </p>
  
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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Latest revision as of 00:31, 19 September 2015




Description

Expression of Salmonella and Vibrio cholerae TolC protein – A western blot was performed using α-TolC specific against Escherichia coli antibodies. These antibodies also recognize the TolC proteins from Salmonella and Vibrio cholerae. Lane 1 contains the protein ladder (NEB Prestained protein ladder, broad range, NEB #7702) which demonstrates the sizes of the TolC proteins displayed in the western blot are correct according to the predicted molecular weights for each. The next two lanes are wild-type E. coli (BW25113) with the chromosomal tolC gene (Lane 2) or without (Lane 3). The following three lanes are the E. coli strains containing the tolC deletion and the pUC57 plasmid with the arabinose promoter alone (Lane 4), the arabinose promoter followed by the Salmonella tolC gene (Lane 5), or the arabinose promoter followed by the Vibrio cholerae tolC gene (Lane 6). No arabinose was added to the cultures used for the samples in Lanes 4, 5, and 6. Lanes 7, 8, and 9 were the same strains as the previous three lanes except that 0.1% arabinose was added to each culture.