Difference between revisions of "Template:Heidelberg/project/rd/results/click"

 
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                                     Entre HERE
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                                     In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.
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                                    <img src="https://static.igem.org/mediawiki/2015/thumb/2/23/Heidelberg_CLT_Fig.4_CuAAC.png/800px-Heidelberg_CLT_Fig.4_CuAAC.png" style="min-width:70%;">
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            <img class="img-responsive" src="https://static.igem.org/mediawiki/2015/thumb/2/23/Heidelberg_CLT_Fig.4_CuAAC.png/800px-Heidelberg_CLT_Fig.4_CuAAC.png">
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                Figure 4. Alkyne modified RNA after click reaction on PAGE
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Left: Gel scanned in Alexa 488 mode on Typhoon scanner. Right: Same gel scanned after SYBR Gold stain. The sample incubated with 5-Ethynyl-dUTP was fluorescently labeled after click reaction. The only band that could be detected in the flourescent scan overlayed with the band of the mentioned sample in the SYBR Gold stain that detects all nucleic acids.
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Latest revision as of 00:36, 19 September 2015

Click Chemistry

In figure 4 one can see three labelling RNAs each having different modifications respectively having either a C8 Alkyne-dUTP, 5-EdUTP or C8 Alkyne-dCTP incorporated. The Alexa 488 scan indicates that one Labelling RNA + 5-EdUTP was modified with an alkyne and that the CuAAC reaction was successful, too.

Figure 4. Alkyne modified RNA after click reaction on PAGE

Left: Gel scanned in Alexa 488 mode on Typhoon scanner. Right: Same gel scanned after SYBR Gold stain. The sample incubated with 5-Ethynyl-dUTP was fluorescently labeled after click reaction. The only band that could be detected in the flourescent scan overlayed with the band of the mentioned sample in the SYBR Gold stain that detects all nucleic acids.