|
|
| (10 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| | {{Template_All_Teams}} | | {{Template_All_Teams}} |
| | {{TecCEM_HS}} | | {{TecCEM_HS}} |
| | + | <div id="Sublinks"> |
| | + | <html> |
| | + | <ul> |
| | + | <li> |
| | + | <a href="https://2015.igem.org/Team:TecCEM_HS/Description"><img src = "https://static.igem.org/mediawiki/2015/f/ff/TecCEM_HS_WIKI_T_Project.png" width=300px heigth=150px /><li></li></a> |
| | + | |
| | + | <br> |
| | + | <a href="https://2015.igem.org/Team:TecCEM_HS/Description"><img src = "https://static.igem.org/mediawiki/2015/b/bf/TecCEM_HS_WIKI_Subtitulos_04.png" width=100px heigth=100px /><li></li></a> |
| | | | |
| | + | <a href="https://2015.igem.org/Team:TecCEM_HS/Design"><img src = "https://static.igem.org/mediawiki/2015/6/6c/TecCEM_HS_WIKI_Subtitulos_07.png" width=100px heigth=100px/><li></li></a> |
| | + | |
| | + | <a href="https://2015.igem.org/Team:TecCEM_HS/Experiments"><img src = "https://static.igem.org/mediawiki/2015/3/33/TecCEM_HS_WIKI_Subtitulos_05.png" width=100px heigth=100px /><li></li></a> |
| | + | |
| | + | <a href="https://2015.igem.org/Team:TecCEM_HS/Results"><img src = "https://static.igem.org/mediawiki/2015/f/fb/TecCEM_HS_WIKI_Subtitulos_06.png" width=100px heigth=100px/><li></li></a> |
| | + | </li> |
| | + | </ul> |
| | + | </html> |
| | + | </div> |
| | + | |
| | + | <div id="contentContainer"> |
| | <html> | | <html> |
| | <head> | | <head> |
| Line 17: |
Line 36: |
| | </head> | | </head> |
| | | | |
| | + | <div class="piktowrapper-embed" pikto-uid="7975709-project-description" > |
| | + | <div class="pikto-canvas-wrap"> |
| | + | <div class="pikto-canvas"></div> |
| | + | </div> |
| | + | </div> |
| | + | <script> |
| | + | (function(d){ |
| | + | var js, id="pikto-embed-js", ref=d.getElementsByTagName("script")[0]; |
| | + | if (d.getElementById(id)) { return;} |
| | + | js=d.createElement("script"); js.id=id; js.async=true; |
| | + | js.src="https://magic.piktochart.com/assets/embedding/embed.js"; |
| | + | ref.parentNode.insertBefore(js, ref); |
| | + | }(document)); |
| | + | </script> |
| | | | |
| − | <h2> Project Description </h2>
| |
| | | | |
| − | <h3> SDSeeker: Bioremediation of Lago de Guadalupe </h3>
| |
| | | | |
| − |
| |
| − | <p>
| |
| − | Lago de Guadalupe is the most extensive water body in the State of Mexico. It is surrounded by industrial, agricultural and residential areas, the last one being responsible for 25% of the total contaminants, among these is SDS (sodium dodecyl sulfate), which is the principal component of detergents used in households. The residues of detergents change the water's pH, turning it into a toxic and dangerous environment for more than 150 species that depend on this lake. Although this lake has been studied for its treatment and to obtain better levels of water quality, nothing has been achieved. Our project will consist in developing a bioremediation system using synthetic biology, to lower SDS levels in the water.
| |
| − | The early stages of this project include capturing SDS molecules with a protein called ferritin and the catalysis of the first step in the SDS degradation pathway, mediated by an alkyl sulfatase.
| |
| − | </p>
| |
| − | <br />
| |
| − |
| |
| − |
| |
| − | <h2>Lago de Guadalupe contamination</h2>
| |
| − | <p/>
| |
| − | Lago de Guadalupe is an artificial lake of 400 hectares located in State of Mexico and is also the largest water body in this area. It is located in the northwest part of the city and it is the habitat of more than 150 species of animals. <br>
| |
| − |
| |
| − | The lake is located in three different zones and each one contributes to the contamination of the lake due to many factors, including home residues and industrial wastes. Some studies confirm that only 26% of the residues are treated and the rest are disposed in many lakes and water bodies causing not only their contamination, but also eutrophication (enrichment of an ecosystem because of the accumulation of nitrogen and phosphorus). Even though mexican organizations and government associations try to keep the water quality in certain levels and try to protect this lake, different studies show high levels of water contamination due to urban residues release (up to 15 million m3 per year), specially in Bosques del Lago, which is the residential zone next to Lago de Guadalupe. Nowadays it is estimated that more than 25% of the total volume of the lake are residues that contribute to its contamination. <br>
| |
| − |
| |
| − | The contamination issues that can be found in this lake are the following:<br>
| |
| − | 1. High eutrophication.
| |
| − | <br>
| |
| − | 2. Trophic values of 87.4 +/- 7.4
| |
| − | <br>
| |
| − | 3. Low water quality. Value corresponding to “highly contaminated”: 26.0 +/- 12.3
| |
| − | <br>
| |
| − | 4. Absence of dissolved oxygen below 3 meters
| |
| − | <br>
| |
| − | 5. Pollutants’ concentrations above the standards of mexican official norms
| |
| − | <br>
| |
| − | 6. High levels of fecal coliforms presence in water <br><br>
| |
| − |
| |
| − | All of the previously mentioned issues don’t let the lake maintain a stable fish population, altering the whole cycle that involves the organisms that live in this lake and directly or indirectly affect other animals in the surroundings. This situation is terribly altered by the growth in the human populations that surrounds Lago de Guadalupe. <br>
| |
| − |
| |
| − | It is important that even with the help of synthetic biology and technology, the population that surrounds this lake understands what is happening and how they can be directly affected by the high levels of contamination. <br>
| |
| − |
| |
| − | Lago de Guadalupe have a highest concentration of pollutants and the reason that we wanted to make our project on this lake is because we all live in nearby areas and it affects us directly, even so, water pollution is a problem that concerns every one of us!<br> </p>
| |
| − |
| |
| − | <h2>SDS</h2>
| |
| − | <p>One of the pollutants that can be found in the residential area next to this lake is detergents.
| |
| − | The detergents residues are often disposed in this lake, provoking changes in the water’s pH and therefore, affecting life in this water body.<br>
| |
| − | Approximately 40% of the detergents content used in this area, are anionic surfactants. The most widely and commonly household detergent used is known as Sodium Dodecyl Sulfate (SDS). This compound is toxic to aquatic animals, including fish and microorganisms like yeast and some bacteria. Toxicity extends to mammals and animals that are part of the circle of life around this lake.</p>
| |
| − |
| |
| − | <h2> SDSeeker: biorremediation</h2>
| |
| − | <p>
| |
| − | To reduce the adverse effects produced by SDS contamination in water bodies, investigations and protocols for its bioremediation have been established in the past years.<br>
| |
| − | However, most of them stayed in the strain characterization phase of detergent-degrading bacteria. When identifying certain strain that is capable of doing this, researchers found themselves with the risk of discharging high amounts of these bacteria and also altering the environment within the water bodies.<br>
| |
| − | This is why our team decided to develop this project using a different approach, where we can use the specific enzymes to degrade this compound.<br>
| |
| − | The early stages of this project include the detection of detergent presence in Lago de Guadalupe and the usage of an enzyme that is able to catalyze the first step in SDS degradation (alkyl sulfatase from <i>Pseudomonas sp.</i>) and another enzyme (human ferritin) that is capable of binding SDS molecules.
| |
| − | <br/>
| |
| − | <h4>References</h4>
| |
| − | <p>
| |
| − |
| |
| − | Sepulveda, J., Hoyos, J., Gutierrez, F.J., et. al. (2013) The impact of anthropogenic pollution on limnological characteristics of a subtropical highland reservoir “Lago de Guadalupe”, Mexico.
| |
| − | <br><br>
| |
| − | Semarnat. (n.d.) Clasificación de tensoactivos. Retrieved from: http://www.semarnat.gob.mx/archivosanteriores/temas/gestionambiental/Materiales%20y%20Actividades%20Riesgosas/sitioscontaminados/GTZ/E-Clasificacion%20de%20Tensoactivos.pdf
| |
| − | <br><br>
| |
| − | UniProt. (2015) Alkyl Sulfatase. Retrieved from: http://www.uniprot.org/uniprot/Q52556
| |
| − | <br><br>KEGG. (2015) Enzyme 3.1.6.19 Retrieved from: http://www.genome.jp/dbget-bin/www_bget?ec:3.1.6.19
| |
| − | <br><br>BRENDA. (2015) Information on EC 3.1.6.19 - R-specific secondary-alkylsulfatase. Retrieved from: http://www.brenda-enzymes.org/enzyme.php?ecno=3.1.6.19
| |
| − | <br><br>
| |
| − | Hagelueken, G., Thorsten, A., Wiehlmann, L., et. al. (2006) The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases. Retrieved from: http://www.researchgate.net/publication/7096154_The_crystal_structure_of_SdsA1_an_alkylsulfatase_from_Pseudomonas_aeruginosa_defines_a_third_class_of_sulfatases
| |
| − |
| |
| − | <br><br>
| |
| − | European Nucleotide Archive. (2015) Pseudomonas sp. Alkylsulfatase. Retrieved from: http://www.ebi.ac.uk/ena/data/view/AAA25989
| |
| − | <br><br>GenBank. (2015) Pseudomonas sp. (strain ATCC 19151) sdsA gene and 11 kd protein, complete cds's; sdsB gene, partial cds. Retrieved from: http://www.ncbi.nlm.nih.gov/nucleotide/151550?report=genbank&log$=nucltop&blast_rank=1&RID=PU6280GR014
| |
| − | <br><br>Maddocks, S.E., and Oyston, P.C. (2008) Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/19047729
| |
| − | <br><br>Jovcic, B., Venturi, V., Davison, J., et. al. (2010) Regulation of the sdsA alkyl sulfatase of Pseudomonas sp. ATCC19151 and its involvement in degradation of anionic surfactants. Retrieved from: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04738.x/full
| |
| − | <br><br>MPBiomedicals. (2014) Methyl green. Retrieved from: http://www.mpbio.com/product.php?pid=04806404
| |
| − | Baker, J. and Williams, E. (1973) The use if methyl green as a histochemical reagent. Retrieved from: http://jcs.biologists.org/content/s3-106/73/3.full.pdf
| |
| − | <br><br>(n.a.) (2005) pH measurements - indicators. Retrieved from: http://www.ph-meter.info/pH-measurements-indicators
| |
| − | State University of New York at Oneonta. (2011) Spectrophotometry. Absorption measurements and their application to quantitative analysis. Retrieved from: http://employees.oneonta.edu/kotzjc/LAB/Spec_intro.pdf
| |
| − | <br><br>
| |
| − | Chaturvedi, V., & Kumar, A. (2010). Bacterial utilization of sodium dodecyl sulfate. International Journal of Applied Biology and Pharmaceutical Technology, 1(3). Retrieved June 5, 2015, from http://ijabpt.org/applied-biology/bacterial-utilization-of-sodium-dodecyl-sulfate.pdf.<br><br>
| |
| − | Sodium lauryl sulfate. (2011). Retrieved June 6, 2015, from http://www.drugbank.ca/drugs/DB00815.<br><br>
| |
| − | Lauril sulfato sódico. (2010). Retrieved June 6, 2015, from http://www.acofarma.com/admin/uploads/descarga/1638-2678f6c4b3f8011c1dc948f03c32eda620e34983/main/files/Lauril_sulfato_sodico.pdf.<br><br>
| |
| − | 1-Dodecanol. (2008). Retrieved June 6, 2015, from http://www.lookchem.com/1-Dodecanol/.
| |
| | <br><br> | | <br><br> |
| | </p> | | </p> |
| − |
| |
| − |
| |
| − |
| |
| − | <h4>Inspiration</h4>
| |
| − | <p>See how other teams have described and presented their projects: </p>
| |
| − |
| |
| − | <ul>
| |
| − | <li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
| |
| − | <li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
| |
| − | <li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
| |
| − | </ul>
| |
| − |
| |
| | </div> | | </div> |
| | </html> | | </html> |
| | + | </div> |
