Difference between revisions of "Team:TCU Taiwan/Result/Peptides"

 
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To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat, and Dermal microvascular endothelium cell called HMEC-1. To make the result closer to human, both cells were sampled from human cells.</br></br>
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To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat. To make the result closer to human, both cells were sampled from human cells.</br></br>
 
Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.</br>
 
Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.</br>
 
In cell migration experiment, samples are divided into following groups:</br>1. Mock</br>2. pQE-60 has no insert and no IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l  (Control 1)</br>3. pQE-60 has insert, without IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l (Control 2)</br>4. pQE-60 has no insert, with IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l (Control 3)</br>5. 1<span style="font-family:Symbol;"></span>l AMPs </br>6. 5<span style="font-family:Symbol;"></span>l AMPs</br>7. 100<span style="font-family:Symbol;"></span>l AMPs</br>8. 200<span style="font-family:Symbol;"></span>l AMPs</br></br>Furthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.
 
In cell migration experiment, samples are divided into following groups:</br>1. Mock</br>2. pQE-60 has no insert and no IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l  (Control 1)</br>3. pQE-60 has insert, without IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l (Control 2)</br>4. pQE-60 has no insert, with IPTG induction of LB medium for 200 <span style="font-family:Symbol;"></span>l (Control 3)</br>5. 1<span style="font-family:Symbol;"></span>l AMPs </br>6. 5<span style="font-family:Symbol;"></span>l AMPs</br>7. 100<span style="font-family:Symbol;"></span>l AMPs</br>8. 200<span style="font-family:Symbol;"></span>l AMPs</br></br>Furthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.
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In Epinecidin-1, concentration of 1ul, we can see the most significant effectiveness. 
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In Signiferin, concentration of 50<span style="font-family:Symbol;"></span>l, we can see the most significant effectiveness.
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<img src="https://static.igem.org/mediawiki/2015/7/78/Tcu_TaiWAn_ResUlt_6.jpeg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/b/b4/Tcu_TaiWAn_ResUlt_7.jpeg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/9/9f/Tcu_TaiWAn_ResUlt_8.jpeg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/3/3a/Tcu_TaiWAn_ResUlt_9.jpeg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/8/81/2015tcutaiwan10.jpg"  width="70%"  align="center" /></br></br>
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</table>
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<br>
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<table width="95%"  align="center">
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<tr>
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<td ><h1><span style="font-family:Calibri;text-align:justify;"><font size="5">
 +
In Epinecidin-1 and Signiferin, concentration of 1<span style="font-family:Symbol;"></span>l, we can see the most significant effectiveness.</br>
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</font></span></h1></td>
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<td width="80%"align="center"><h1><span style="font-family:Calibri;text-align:justify;"><font size="5">
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<img src="https://static.igem.org/mediawiki/2015/a/a4/2015tcutaiwan12.jpg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/e/ed/2015tcutaiwan13.jpg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/1/1c/2015tcutaiwan14.jpg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/c/c5/2015tcutaiwan15.jpg"  width="70%"  align="center" /></br></br>
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<img src="https://static.igem.org/mediawiki/2015/7/77/2015tcutaiwan16.jpg"  width="70%"  align="center" /></br></br>
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</font></span></h1>
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</table>
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<div id="st1" class="st">
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<div class="inner">
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<table width="95%"  align="center">
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<tr><td><h1><span style="font-family:Calibri;text-align:justify;"><font size="5">
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After cell migration experiment, To interpret our result, we use ImageJ to distance measurement and compare them. </br>
 
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Latest revision as of 01:31, 19 September 2015

To indirect prove wound healing, we perform experiments in cell migration. Two cells are selected for the experiment: Squamous keratinocyte called HaCat. To make the result closer to human, both cells were sampled from human cells.

Squamous keratinocyt cell shows the influence of our peptides on epithelial. On other hand, because angiogenesis is one of the indicators of wound healing, Dermal microvascular endothelium cell is selected here.
In cell migration experiment, samples are divided into following groups:
1. Mock
2. pQE-60 has no insert and no IPTG induction of LB medium for 200 l (Control 1)
3. pQE-60 has insert, without IPTG induction of LB medium for 200 l (Control 2)
4. pQE-60 has no insert, with IPTG induction of LB medium for 200 l (Control 3)
5. 1l AMPs
6. 5l AMPs
7. 100l AMPs
8. 200l AMPs

Furthermore, all of above groups are divided into three more groups: Epinecidin-1, Signiferin, Epinecidin-1 and Signiferin.

In Epinecidin-1, concentration of 1ul, we can see the most significant effectiveness.












In Signiferin, concentration of 50l, we can see the most significant effectiveness.












In Epinecidin-1 and Signiferin, concentration of 1l, we can see the most significant effectiveness.











After cell migration experiment, To interpret our result, we use ImageJ to distance measurement and compare them.



             
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