Difference between revisions of "Team:TCU Taiwan/Description"
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− | Signiferin is a peptide came from the skin mucus of Crinia signifera. It demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has already been demonstrated by the TU-Delft 2013 iGEM team. <a href="#tcu_references_3">[3]</a> Epinecidin-1 is a peptide came from the skin mucus of Epinephelus coioides. It has ability to help wound healing and has been proven by animal studies, and was selected as an additional reagent. <a href="#tcu_references_4">[4]</a> By combining these two properties, we believe that can develop a wound dressing that may be useful in trauma patients without the additional risk of developing drug-resistance.<br><br> | + | Signiferin is a peptide came from the skin mucus of<I> Crinia signifera</I>. It demonstrated effectiveness in killing Methicillin-Resistant <I>Staphylococcus aureus</I> (MRSA), and has already been demonstrated by the TU-Delft 2013 iGEM team. <a href="#tcu_references_3">[3]</a> Epinecidin-1 is a peptide came from the skin mucus of <I>Epinephelus coioides</I>. It has ability to help wound healing and has been proven by animal studies, and was selected as an additional reagent. <a href="#tcu_references_4">[4]</a> By combining these two properties, we believe that can develop a wound dressing that may be useful in trauma patients without the additional risk of developing drug-resistance.<br><br> |
− | To control the AMPs expression and secretion, the Lac operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of | + | To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of <I>S. aureus</I> and <I>in vitro</I> wound healing assay for epithelial cells line (HaCaT). Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process. |
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<td style="vertical-align:text-top;">1.</td> | <td style="vertical-align:text-top;">1.</td> | ||
− | <td wigth="80%">Helps AMPs to secret out of | + | <td wigth="80%">Helps AMPs to secret out of <I>E. coli</I>.</td> |
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<td style="vertical-align:text-top;">2.</td> | <td style="vertical-align:text-top;">2.</td> | ||
− | <td wigth="80%">From <I>Streptomyces lividans</I> to | + | <td wigth="80%">From <I>Streptomyces lividans</I> to transport chitinase C to secretion system, which has been proven to work in <I>E.coli</I> by reference.</td> |
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<td style="vertical-align:text-top;" align="left">[1]</td> | <td style="vertical-align:text-top;" align="left">[1]</td> | ||
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− | Yeaman, M.R. and N.Y. Yount, Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev, 2003. 55(1): p. 27-55. | + | Yeaman, M.R. and N.Y. Yount, <I>Mechanisms of antimicrobial peptide action and resistance</I>. Pharmacol Rev, 2003. 55(1): p. 27-55. |
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<td style="vertical-align:text-top;" align="left">[2]</td> | <td style="vertical-align:text-top;" align="left">[2]</td> | ||
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− | Lai, Y. and R.L. Gallo, AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol, 2009. 30(3): p. 131-41. | + | Lai, Y. and R.L. Gallo, <I>AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense</I>. Trends Immunol, 2009. 30(3): p. 131-41. |
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− | <td style="vertical-align:text-top;" align="left">[3]</td> | + | <td width="11%"style="vertical-align:text-top;white-space: nowrap;" align="left" >[3]</td> |
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− | TU_Delf, i.t. 2013; Available from: https://2013.igem.org/Team:TU-Delft. | + | TU_Delf, i.t. 2013; Available from: <a href="https://2013.igem.org/Team:TU-Delft">https://2013.igem.org/Team:TU-Delft</a>. |
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<a name="tcu_references_5"></a> | <a name="tcu_references_5"></a> | ||
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<td style="vertical-align:text-top;" align="left">[4]</td> | <td style="vertical-align:text-top;" align="left">[4]</td> | ||
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− | Huang, H.N., et al., Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries. Biomaterials, 2013. 34(38): p. 10319-27. | + | Huang, H.N., et al., <I>Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries</I>. Biomaterials, 2013. 34(38): p. 10319-27. |
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<td style="vertical-align:text-top;" align="left">[5]</td> | <td style="vertical-align:text-top;" align="left">[5]</td> | ||
<td width="90%"> | <td width="90%"> | ||
− | Tokuyasu, K., et al., Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells. FEBS Lett, 1999. 458(1): p. 23-6. | + | Tokuyasu, K., et al., <I>Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells</I>. FEBS Lett, 1999. 458(1): p. 23-6. |
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<td style="vertical-align:text-top;" align="left">[6]</td> | <td style="vertical-align:text-top;" align="left">[6]</td> | ||
<td width="90%"> | <td width="90%"> | ||
− | Fujii, T. and K. Miyashita, Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J Gen Microbiol, 1993. 139(4): p. 677-86. | + | Fujii, T. and K. Miyashita, <I>Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans</I>. J Gen Microbiol, 1993. 139(4): p. 677-86. |
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Latest revision as of 01:32, 19 September 2015
![](https://static.igem.org/mediawiki/2015/5/5f/2015tcutaiwanProject.jpg)
AMP. coli |
To achieve our goal we incorporated antimicrobial peptides (AMPs) into our medical dressing. AMPs, are stable peptide that have extensive ability in bactericidal effects. Unlike antibiotics, AMPs can puncture the cell membrane to kill the bacteria therefore bypassing bacterial antibiotic drug resistance mechanisms. [1] Besides, the peptides also have ability to help skin recovered. [2] After reading numerous of research articles, we selected two kinds of AMPs: Signiferin and Epinecidin-1 as our reagents. |
• Epinecidin-1:
• Signiferin:
Based on AMPs to develop into a potential material of wound dressing. |
References
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Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |