Difference between revisions of "Team:TCU Taiwan/Description"
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− | To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of | + | To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of <I>S. aureus</I> and <I>in vitro</I> wound healing assay for epithelial cells line (HaCaT). Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process. |
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− | <td wigth="80%">From <I>Streptomyces lividans</I> to | + | <td wigth="80%">From <I>Streptomyces lividans</I> to transport chitinase C to secretion system, which has been proven to work in <I>E.coli</I> by reference.</td> |
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TU_Delf, i.t. 2013; Available from: <a href="https://2013.igem.org/Team:TU-Delft">https://2013.igem.org/Team:TU-Delft</a>. | TU_Delf, i.t. 2013; Available from: <a href="https://2013.igem.org/Team:TU-Delft">https://2013.igem.org/Team:TU-Delft</a>. | ||
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Latest revision as of 01:32, 19 September 2015
![](https://static.igem.org/mediawiki/2015/5/5f/2015tcutaiwanProject.jpg)
AMP. coli |
To achieve our goal we incorporated antimicrobial peptides (AMPs) into our medical dressing. AMPs, are stable peptide that have extensive ability in bactericidal effects. Unlike antibiotics, AMPs can puncture the cell membrane to kill the bacteria therefore bypassing bacterial antibiotic drug resistance mechanisms. [1] Besides, the peptides also have ability to help skin recovered. [2] After reading numerous of research articles, we selected two kinds of AMPs: Signiferin and Epinecidin-1 as our reagents. |
• Epinecidin-1:
• Signiferin:
Based on AMPs to develop into a potential material of wound dressing. |
References
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Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |