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| − | <h3 class="basicheader"> TITLE </h3>
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| | <div class="panel‐body"> | | <div class="panel‐body"> |
| | <div class="row"> | | <div class="row"> |
| − | <div class="row" style="margin-top: 20px; background-color:white"> | + | <div class="col-lg-12"> |
| − | </div>
| + | <h3 class="basicheader"> |
| − | <div class="row" style="margin:0;">
| + | We established a NEW APPROACH to work with all types of functional nucleic acids, setting the foundations for their widespread use in synthetic biology |
| − | <div class="col-md-3 col-xs-12 vcenter-cell"> | + | </h3> |
| − | <img src="https://static.igem.org/mediawiki/2014/a/ae/Achievements_3.png" class="img-responsive" style="margin-left:15px;">
| + | <ul> |
| − | </div>
| + | <li><p class="basictext">We developed a NEW STANDARD to easily clone functional RNAs for their expression in vitro and in vivo (RFC 110) |
| | + | </p></li> |
| | + | <li><p class="basictext">We established a NOVEL dual-color readout system to monitor the in vitro transcription of an RNA of interest based on a new ATP-switchable Spinach II aptamer |
| | | | |
| − | <div class="col-md-9 col-xs-12 vcenter-cell">
| + | </p></li> |
| − | <div class="col-xs-9">
| + | < li><p class=" basictext"> We established a NOVEL detection method for short ssDNAs and ssRNAs based on the HRP-mimicking DNAzyme |
| − | <p style="font-weight:light; font-size:25px">Establishing protein circularization as a NEW BIOENGINEERING TOOL in synthetic biology.</p>
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| − | <p style="margin-left:50px; font-size:20px">Contributing to iGEM with a new foundational advance!</p>
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| − | </div>
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| − | <div class="col-xs-3">
| + | </p></li> |
| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
| + | <li><p class="basictext">We designed de novo an ACTIVE SITE for twin ribozyme and proved its efficient cleavage activity in living yeast cells |
| − | </div>
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| | | | |
| − | </div>
| + | </p></li> |
| − | </div>
| + | <li><p class="basictext">We conceived and implemented an open-source SOFTWARE FOR THE DESIGN OF APTAMERS (MAWS) as a fast and affordable alternative to the laborious SELEX procedure |
| − | <div class="linie"></div>
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| | | | |
| − | <div class="row" style="margin:0;">
| + | </p></li> |
| − | <div class="col-md-3 col-xs-12 vcenter-cell">
| + | <li><p class="basictext">We conceived and implemented an open-source SOFTWARE ASSISTING THE DESIGN OF APTAZYMES (JAWS) to enable the construction of new sensing devices |
| − | <img src="https://static.igem.org/mediawiki/2014/1/11/Achievement_1.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
| + | </p></li> |
| − | <div class="col-xs-9">
| + | < li><p class=" basictext"> We EXPERIMENTALLY VALIDATED several of our designed aptamers and aptazymes and fed the collected data back into the algorithms |
| − | <p style="font-weight:light; font-size:25px">Providing a NEW COMPREHENSIVE TOOLBOX based on inteins for modifying proteins post-translationally.</p>
| + | |
| − | <p style="margin-left:50px; font-size:20px">Sending 67 Biobricks to Registry of Biological parts!</p>
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| − | </div>
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| − | <div class="col-xs-3">
| + | </p></li> |
| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
| + | </ul> |
| − | </div>
| + | <br/> |
| − | | + | <h3 class="basicheader"> |
| − | </div>
| + | We proved the PRINCIPLE that a ketamine-switchable aptazyme could be used to implement a TEST STRIPE to detect RAPE DRUGS in situ |
| − | </div>
| + | </h3> |
| − | <div class="linie"></div>
| + | <br/> |
| − | | + | <h3 class="basicheader"> |
| − | <div class="row vcenter-table" style="margin:0;">
| + | We developed the APTABODY, an aptamer-based alternative to antibodies that drastically reduces the costs of performing WESTERN BLOTTING and allows to target proteins for which antibodies are not available |
| − | <div class="col-md-3 col-xs-12 vcenter-cell">
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| − | <img src="https://static.igem.org/mediawiki/2014/f/f9/Achievement_2.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <div class="col-xs-9">
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| − | <p style="font-weight:light; font-size:25px">Development of a NEW STANDARD to make the use of inteins easy and modular.</p>
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| − | <p style="margin-left:50px; font-size:20px">Establishment of a new <a href="https://2014.igem.org/Team:Heidelberg/Parts/RFC">RFC</a>!</p>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | </div>
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| − | </div>
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| − | <div class="linie"></div>
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| − | <div class="col-md-3 col-xs-12 vcenter-cell">
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| − | <img src="https://static.igem.org/mediawiki/2014/1/1b/Achievements_toolbox.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <div class="col-xs-9">
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| − | <p style="font-weight:light; font-size:25px">Showing that the toolbox WORKS: proteins are circularized and split fluorescent proteins are reconstituted.</p>
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| − | <p style="margin-left:50px; font-size:20px">Making Gels, Western Blots, Fluorescence-based Assays and Mass spectrometry to prove it! </p>
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| − | </div>
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| − | <div class="col-xs-3">
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | </div>
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| − | </div>
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| − | <div class="linie"></div>
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| − | <img src="https://static.igem.org/mediawiki/2014/2/26/Achievements_4.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <div class="col-xs-9">
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| − | <p style="font-weight:light; font-size:25px">Creating circular DNMT1 and showing that it is ACTIVE.</p>
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| − | <p style="margin-left:50px; font-size:20px">For the first time achieving the circularization of a large protein!</p>
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| − | <p style="margin-left:50px; font-size:20px">Circularizing LYSOZYME and XYLANASE, two very important proteins for
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| − | research and industry!</p>
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| − | </div>
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| − | <div class="col-xs-3">
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="linie"></div>
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| − | <div class="row vcenter-table" style="margin:0;">
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| − | <div class="col-md-3 col-xs-12 vcenter-cell">
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| − | <img src="https://static.igem.org/mediawiki/2014/5/5a/Achievements_craut.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <p style="font-weight:light; font-size:25px">Developing a NEW SOFTWARE to calculate customized linkers to circularize proteins.</p>
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| − | <p style="margin-left:50px; font-size:20px">Making CRAUT open-source for the scientific community.</p>
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| − | </div>
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| − | <div class="col-xs-3">
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | </div>
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| − | </div>
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| − | <div class="linie"></div>
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| − | <div class="row vcenter-table" style="margin:0;">
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| − | <div class="col-md-3 col-xs-12 vcenter-cell">
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| − | <img src="https://static.igem.org/mediawiki/2014/e/e5/Heidelberg_Frontpage_igemathome.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <div class="col-xs-9">
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| − | <p style="font-weight:light; font-size:25px"> Establishing a distributed computing platform called <a href="https://2014.igem.org/Team:Heidelberg/Software/igemathome">iGEM@home</a>.</p>
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| − | <p style="margin-left:50px; font-size:20px">Using this platform as an entirely new way to reach out to the world with synthetic biology concepts!</p>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | </div>
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| − | <div class="linie"></div>
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| − | <div class="col-md-3 col-xs-12 vcenter-cell">
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| − | <img src="https://static.igem.org/mediawiki/2014/1/18/Achievements_md.png" class="img-responsive" style="margin-left:15px;">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12 vcenter-cell">
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| − | <p style="font-weight:light; font-size:25px">Creating a NEW SOFTWARE to display the notebook on the wiki.</p>
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| − | <p style="margin-left:50px; font-size:20px">Distributing MidNightDOC to the iGEM community to help future teams organize their protocols!</p>
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| − | </div>
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| − | <div class="col-xs-3">
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| − | <img src="https://static.igem.org/mediawiki/2015/a/ab/Heidelberg_media_icons_anchor_green.png" style="height:100px; margin-right:15px;">
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| − | <div class="tab-pane fade" style="color:white;" id="Medal_Criteria-tab">
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| − | <img class="medal" src="https://static.igem.org/mediawiki/2014/6/63/Heidelberg_Bronze.png">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12">
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| − | <h1>Bronze</h1>
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| − | <br>
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| − | <ul>
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| − | <li>Please find a comprehensive compilation of <a href="https://2014.igem.org/Team:Heidelberg/Team/Sponsoring">sponsors</a>, partners and scientific contributors on our <a href="https://2014.igem.org/Team:Heidelberg/Team/Attributions">acknowledgements page</a>. </li>
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| − | <br/>
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| − | <li>We also encourage you to take notice of the projects “Photo-intein” and “Mito-intein” by <a href="https://2014.igem.org/Team:Queens_Canada/Project">iGEM team Queens from Canada</a> that may supply you with complementary information and tools for the use of inteins in synthetic biology!</li>
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| − | <br/>
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| − | <li>A list of links to more than 60 parts in the registry submitted by our team (being or not being part of the new intein toolbox) can be found <a href="https://2014.igem.org/Team:Heidelberg/Parts#allParts">here</a>.</li>
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| − | </ul>
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| − | </div>
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| − | <img class="medal" src="https://static.igem.org/mediawiki/2014/b/bf/Heidelberg_Silver.png">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12">
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| − | <h1>Silver</h1>
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| − | <br>
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| − | <ul>
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| − | <li>We experimentally validated that our biobricks <a href="http://parts.igem.org/Part:BBa_K1362000">BBa_K1362000</a>, <a href="http://parts.igem.org/Part:BBa_K1362100">BBa_K1362100 </a>and <a href="http://parts.igem.org/Part:BBa_K1362101">BBa_K1362101</a> work as expected. For more information on the <a href="https://2014.igem.org/Team:Heidelberg/Parts#Favorite Parts">parts</a> please visit the corresponding main pages in the parts registry or explore their involvement in our subprojects.</li>
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| − | <br/>
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| − | <li>Religious perceptions of synthetic biology have been part of several surveys during the past ten years of iGEM and Human Practices projects. Since religious groups cover the majority of worlds population, deliver moral values and wield power at the same time, we decided to dedicate a whole event on the topic of religion, philosophy and ethics regarding synthetic biology. Please find an <a href="https://2014.igem.org/Team:Heidelberg/Human_Practice/Ethics">evaluation of our event</a> on the corresponding Human Practices pages. <!--In order to reassure ourselves about the acceptability of our project and synthetic biology in general, we also used this opportunity to build up on the work of the iGEM Team Heidelberg 2013 and conducted a survey that addresses basic questions regarding the public reflection of our work.-->
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| − | </li>
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| − | </ul>
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| − | <div class="col-md-3 col-xs-12">
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| − | <img class="medal" src="https://static.igem.org/mediawiki/2014/0/0a/Heidelberg_Gold.png">
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| − | </div>
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| − | <div class="col-md-9 col-xs-12">
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| − | <h1>Gold</h1>
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| − | <ul>
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| − | <li>We improved the function of the <b>already existing</b> biobrick part <a href="http://parts.igem.org/Part:BBa_K117505">BBa_K1175005 </a>by optimizing and resubmitting the corresponding sequence of B. subtilis xylanase to the registry (Part:<a href="http://parts.igem.org/Part:BBa_K1362020"> BBa_K1362020</a>). In addition, we submitted a new part for expression of <a href="http://parts.igem.org/Part:BBa_K1362022">circularized xylanase </a>(BBa_K1362022) that might be used in future applications with need for refined enzyme stability.</li>
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| − | <br/>
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| − | <li>Despite the fact that we focused on building a set of powerful soft- and wetware tools to help future iGEM-teams developing and realizing projects in synthetic biology, we are happy to announce that we were also able to help out several team during the course of our project, aspecially with sending <a href="https://2014.igem.org/Team:Heidelberg/Parts#Backbones"> our expression vectors</a>. Read more abou in in our <a href="https://2014.igem.org/Team:Heidelberg/Team/Collaborations">Collaborations</a>.</li>
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| − | <br/>
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| − | <li>In the style of of the new iGEM community labs track that involves science amateurs “beyond the accolades of scientific publishing and economic reward”, we sought for a new way to involve laymen in actual science and build a strong community of well informed supporters and communicators of synthetic biology at the same time. Now we proudly present the crowd sourcing and communication platform <a href="https://2014.igem.org/Team:Heidelberg/Human_Practice/igemathome">iGEM@home</a>.</li>
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| − | </ul>
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