Difference between revisions of "Team:IISER Pune/Measurement"

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<h2> Measurement</h2>
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<center><img src="https://static.igem.org/mediawiki/2015/2/21/Team_IISER_Pune_TT7.gif"></img></center><hr />
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<h3>INTER LAB STUDY</h3>
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<p>Our team participated in second interlab study program. The data of Interlab study is provided below</p>
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<a href="https://static.igem.org/mediawiki/2015/0/00/IISER_PUNE_INterlab.pdf">Interlab Study Data</a>
  
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<h3>Characterized existing BioBricks</h3>
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<p>The biobricks BBa_K274110,BBa_K274120,BBa_K274210,BBa_K274220 were characterized as the part of Detection module</p>
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<h4>Note</h4>
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<table style="background-color:#FFF4AA" border="1">
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Measurement Approach award</a>, you must fill out this page.</p>
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<tr>
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<th>BioBrick number</th>
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<th>Description</th>
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<p>There are a lot of exciting Parts in the Registry, but many Parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<td><a href="http://parts.igem.org/Part:BBa_K274110">BBa_K274110</a></td>
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<td> lacI+pL promoter + Lycopene</td>
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<h4>Inspiration</h4>
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<p>You can look at what other teams did to get some inspiration! <br />
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<td><a href="http://parts.igem.org/Part:BBa_K274120">BBa_K274120</a></td>
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Here are a few examples:</p>
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<td>pBAD/araC promoter + Lycopene</td>
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<ul>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus">2014 Valencia Biocampus</a></li>
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<td><a href="http://parts.igem.org/Part:BBa_K274210">BBa_K274210</a></td>
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</ul>
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<td> lacI+pL promoter + beta-carotene</td>
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<td><a href="http://parts.igem.org/Part:BBa_K274220">BBa_K274220</a></td>
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<td> lacI+pL promoter + beta-carotene</td>
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</tr>
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</table>
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<h2>Characterization by Microscopy</h2>
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<p>Microscopy was done using brightfeild microscope with colored camera. Cells were induced at the time of sub-culturing Cells were fixed using Fixation protocol.</p>
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<table style="background-color:#FFF4AA">
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<tr><td>Control cells</td></tr>
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<td><img src="https://static.igem.org/mediawiki/2015/6/6c/IISER_PUNE_M_control.jpg" width=300px></td>
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</tr>
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<tr><td>In NEB Top10 cells</td></tr>
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<td><img src="https://static.igem.org/mediawiki/2015/a/a5/IISER_PUNE_M_T110.jpg" width=300px></td>
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<td><img src="https://static.igem.org/mediawiki/2015/8/81/IISER_PUNE_M_T120.jpg" width=300px></td>
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</tr>
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<td><img src="https://static.igem.org/mediawiki/2015/d/d6/IISER_PUNE_M_T210.jpg" width=300px></td>
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<td><img src="https://static.igem.org/mediawiki/2015/8/8d/IISER_PUNE_M_T220.jpg" width=300px></td>
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</tr>
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<tr><td>In E.coli MG1655 cells</td></tr>
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<td><img src="https://static.igem.org/mediawiki/2015/3/36/IISER_PUNE_M_MG110.jpg" width=300px></td>
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<td><img src="https://static.igem.org/mediawiki/2015/f/fc/IISER_PUNE_M_MG120.jpg" width=300px></td>
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</tr>
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<td><img src="https://static.igem.org/mediawiki/2015/5/58/IISER_PUNE_M_MG210.jpg" width=300px></td>
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<td><img src="https://static.igem.org/mediawiki/2015/d/d5/IISER_PUNE_M_MG220.jpg" width=300px></td>
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</tr>
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</table>
  
 
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Latest revision as of 01:34, 19 September 2015


Top

INTER LAB STUDY

Our team participated in second interlab study program. The data of Interlab study is provided below

Interlab Study Data

Characterized existing BioBricks

The biobricks BBa_K274110,BBa_K274120,BBa_K274210,BBa_K274220 were characterized as the part of Detection module

BioBrick number Description
BBa_K274110 lacI+pL promoter + Lycopene
BBa_K274120 pBAD/araC promoter + Lycopene
BBa_K274210 lacI+pL promoter + beta-carotene
BBa_K274220 lacI+pL promoter + beta-carotene

Characterization by Microscopy

Microscopy was done using brightfeild microscope with colored camera. Cells were induced at the time of sub-culturing Cells were fixed using Fixation protocol.

Control cells
In NEB Top10 cells
In E.coli MG1655 cells