Difference between revisions of "Team:OUC-China"

 
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
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              <td><a href="#">home</a></td>
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              <td><a href="#">project</a></td>
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            <h2>Magthermo coli</h2>
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              <td><a href="#">notebook</a></td>
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              <td><a href="#">team</a></td>
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                    how does it <span>work?</span>
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                </h2>
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      <div class="sectionTittle">Abstract</div>
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                <p>
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        <div class="sectionTxt2">Gene regulation has typically employed chemically mediated expression systems, which are slow and could be limited by medium during drug diffusion. The development of remote regulation systems that could response to physical signals is in demand.
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                    This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
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This summer, we are trying to design a platform responsed to electromagnetic signal. This platform is composed of 2 sessions : magnetic receiver and thermosensitive regulator. </div>
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                    <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 1</h3>
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  </div>
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                    <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
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  <div class="section section-3">
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    <div class="sectionTxtBox3">
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                <div class="col-md-4">
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      <div class="sectionTittle">Receiver</div>
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                    <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
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        <div class="sectionTxt3">For magnetic receiver, we chose ferritin, the iron-storage protein in organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, which would trigger thermosensitive regulator. </div>
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                    <h3 class="text-uppercase text-center">step 2</h3>
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    </div>
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                    <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
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                    <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive">
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      <div class="sectionTittle">Regulator</div>
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                    <h3 class="text-uppercase text-center">step 3</h3>
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        <div class="sectionTxt4">For thermosensitive regulator, we chose RNA thermometer and constructed a thermosensitive T7 RNA polymerase. RNA thermometer is a structured RNA which could expose RBS only when heated. The thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at the selected locus of which was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.</div>
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                        In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
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            <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3>
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                            For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
 +
                        </p>
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                        <p>
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                            In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
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            <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3>
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            <p class="text-center">For thermosensitive regulator，we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B>  </p>
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                            <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
 +
                        </p>
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                            <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
 +
                        </p>
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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Latest revision as of 01:50, 19 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

More details