Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/tasA Digestion t79"

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{{Team:Groningen/TEMPLATES/LOG
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|title=00:00, 19 August 2015 - 00:00, 19 August 2015
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|title=tasA Digestion (t79)
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|executor=Marieke Mulder
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|protocol=Restriction
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|content=<div class="text">The following constructs were digested: the salt promoter in pSB1C3 with SpeI and PstI, and tasA in pSB1C3 with XbaI and PstI. After digestion the constructs were put on an 1% agarose gel.</div>
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|protocolurl=restriction
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|goal=<html>Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.</html>
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|description=The restriction was performed to make ligation possible.
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|conclusion=The restriction of tasA and the salt promoter was performed.
 
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Latest revision as of 02:19, 19 September 2015

tasA Digestion (t79)
The restriction was performed to make ligation possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The restriction of tasA and the salt promoter was performed.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>

00:00, 19 August 2015 - 00:00, 19 August 2015
The following constructs were digested: the salt promoter in pSB1C3 with SpeI and PstI, and tasA in pSB1C3 with XbaI and PstI. After digestion the constructs were put on an 1% agarose gel.
Marieke Mulder