Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/tasA Digestion t79"
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− | |title= | + | |title=tasA Digestion (t79) |
− | | | + | |protocol=Restriction |
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+ | |goal=<html>Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.</html> | ||
+ | |description=The restriction was performed to make ligation possible. | ||
+ | |conclusion=The restriction of tasA and the salt promoter was performed. | ||
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Latest revision as of 02:19, 19 September 2015
tasA Digestion (t79)
The restriction was performed to make ligation possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The restriction of tasA and the salt promoter was performed.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 19 August 2015 - 00:00, 19 August 2015
The following constructs were digested: the salt promoter in pSB1C3 with SpeI and PstI, and tasA in pSB1C3 with XbaI and PstI. After digestion the constructs were put on an 1% agarose gel.