Difference between revisions of "Team:Bordeaux"

 
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<h2> Welcome to iGEM 2015! </h2>
 
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
  
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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            <h3>The Problem: Downy Mildew</h3>
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<h4> Styling your wiki </h4>
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        <h5 align="justify"> We all love wine , right? </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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        <br>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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        <h6 align="justify"> France is the first producers and exporters of wine, representing approximately 16% of the world production. In fact, Aquitaine is one of the 4 regions which produces the most wine. However, vineyards are threatened by an oomycete called <b> <i> Plasmopara viticola </i> </b>, otherwise known as <b>Downy Mildew </b> which affects the quality of wine by causing irreparable damages to grapevine organs. This microorganism infects the grapevine’s tissues, particularly it's leaves, draining the plant’s resources and preventing its photosynthetic capacities. </h6> 
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        <h6 align="justify"> The most common preventive treatment used by vineyards in the region is called <b> «bouillie bordelaise» </b> and is composed of <b> copper sulfate </b>, a product which has <b> toxic effects </b> on the surrounding soil. Although this treatment is efficient and does help to protect the plant from harmful microorganisms, this  method  has a  <b> bad environmental  and  sanitary impact </b> since the copper present in the treatment infiltrates in the ground and prevents the growth of other types of plants, therefore impacting the biodiversity of the region. Furthermore,  long term contact with these chemical components can  create symptoms such as headaches, stomachaches, or renal/hepatic dysfunctions for those living in these contaminated areas.</h6>
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        <p align ="left" size="12"> <b> <i> For more information see our Problem Page <a href="https://2015.igem.org/Team:Bordeaux/Problem"> &#9755; click here </b> </a> </i> </p>
  
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Bordeaux&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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<h4>Templates </h4>
 
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
 
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
 
  
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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                    <h3> Curdlan: Our Solution  </h3>
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                    <h6 align="justify" > This year, iGEM Bordeaux proposes an <b> eco-friendly alternative </b> treatment to prevent Downy Mildew: replacing copper sulfate by <b> Curdlan </b>, a sugar biopolymer which <b> stimulates the plant natural defenses </b>.<h6>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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                    <h6 align="justify"> The plant naturally defends itself against pathogens through two distinct levels of defenses, an active and a passive defense which both cause a depolarization of the infected cell membrane and trigger the production of antimicrobial metabolites. Curdlan is recognised by the passive defense and activates it, with mechanisms similar to modern day vaccines. <h6>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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                    <p align ="left" size="12"> <b> <i> For more information see our Description Page <a href="https://2015.igem.org/Team:Bordeaux/Description"> &#9755; click here </b> </a> </i> </p>
  
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<h4>Inspiration</h4>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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<h4> Uploading pictures and files </h4>
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        <img style= "width:50vw; height:32vw;" src= "https://static.igem.org/mediawiki/2015/thumb/1/12/IGEM_Bordeaux_Curd-vine-caroline.png/800px-IGEM_Bordeaux_Curd-vine-caroline.png" >  
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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          <h3 > The organisms </h3>
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          <h6 align="justify"> The production of our molecule will be done using <b> non-pathogenic micro-organisms </b> which can easily be grown in our lab: <i> Saccharomyces cerevisiae </i> which naturally produces Curdlan and <i> Escherichia coli </i>, the most common organism used for synthetic biology.</h6>
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          <h6 align="justify"> Our bacterial genes and promoters will be synthethized industrially by IDT before being inserted in <i> Escherichia coli </i> whereas for <i> Saccharomyces cerevisiae </i> the gene will be extracted and inserted into a plasmid under the control of a different promoter. These modified cells will then be cultivated to produce our Curdlan molecule in large quantities. The final step of our project will be to extract the curdlan molecule, to optimize our production and to sulfate the molecule if possible since this enhances it's effect on the plant natural defenses. </h6>
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<img style= "width:50vw; height:35vw;" src="https://static.igem.org/mediawiki/2015/thumb/3/38/SCHEMA_SIMPLE_WIKI_iGEM_2015.jpg/800px-SCHEMA_SIMPLE_WIKI_iGEM_2015.jpg">
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            <h3 style= "color:#ff9a00"> Team members </h3>
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<h5> Who is going to do all of this you ask? </h5>
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<h6 align="justify"> Well, this year, iGEM Bordeaux's team is made up of <b> 19 devoted students </b> from diverse backgrounds. Whether we become from the Bordeaux University, from a Biotechnologies Engineering School or from a Biochemistry Engineering School, we are all united for this unique project and are willing to give it our all to make it succeed! </h6>
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<h6 align="justify"> In order to do so, we united our scientific knowledge and contacted various researchers to put together our protocols. At the same time, our team has spent time and energy thinking about various non-scientific aspects of our project including how to popularize scientific knowledge to raise awareness on the possibilities of synthetic biology. We have also considered throughout the project ethical and bio-safety thematics in order to insure that our protocols are not hazardous for the environment and that any genetically engineered microorganism is controled within the lab. </h6>
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        <p align ="left" size="12"> <b> <i> For more information look at our Team Page <a href="https://2015.igem.org/Team:Bordeaux/Team"> &#9755; click here </b> </a> </i> </p>
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Latest revision as of 02:25, 19 September 2015

IGEM Bordeaux 2015

IGEM Bordeaux 2015

Cur'd Vine

The Problem: Downy Mildew

We all love wine , right?

France is the first producers and exporters of wine, representing approximately 16% of the world production. In fact, Aquitaine is one of the 4 regions which produces the most wine. However, vineyards are threatened by an oomycete called Plasmopara viticola , otherwise known as Downy Mildew which affects the quality of wine by causing irreparable damages to grapevine organs. This microorganism infects the grapevine’s tissues, particularly it's leaves, draining the plant’s resources and preventing its photosynthetic capacities.

The most common preventive treatment used by vineyards in the region is called «bouillie bordelaise» and is composed of copper sulfate , a product which has toxic effects on the surrounding soil. Although this treatment is efficient and does help to protect the plant from harmful microorganisms, this method has a bad environmental and sanitary impact since the copper present in the treatment infiltrates in the ground and prevents the growth of other types of plants, therefore impacting the biodiversity of the region. Furthermore, long term contact with these chemical components can create symptoms such as headaches, stomachaches, or renal/hepatic dysfunctions for those living in these contaminated areas.

For more information see our Problem Page ☛ click here

Curdlan: Our Solution

This year, iGEM Bordeaux proposes an eco-friendly alternative treatment to prevent Downy Mildew: replacing copper sulfate by Curdlan , a sugar biopolymer which stimulates the plant natural defenses .
The plant naturally defends itself against pathogens through two distinct levels of defenses, an active and a passive defense which both cause a depolarization of the infected cell membrane and trigger the production of antimicrobial metabolites. Curdlan is recognised by the passive defense and activates it, with mechanisms similar to modern day vaccines.

For more information see our Description Page ☛ click here

The organisms

The production of our molecule will be done using non-pathogenic micro-organisms which can easily be grown in our lab: Saccharomyces cerevisiae which naturally produces Curdlan and Escherichia coli , the most common organism used for synthetic biology.
Our bacterial genes and promoters will be synthethized industrially by IDT before being inserted in Escherichia coli whereas for Saccharomyces cerevisiae the gene will be extracted and inserted into a plasmid under the control of a different promoter. These modified cells will then be cultivated to produce our Curdlan molecule in large quantities. The final step of our project will be to extract the curdlan molecule, to optimize our production and to sulfate the molecule if possible since this enhances it's effect on the plant natural defenses.

Team members

Who is going to do all of this you ask?
Well, this year, iGEM Bordeaux's team is made up of 19 devoted students from diverse backgrounds. Whether we become from the Bordeaux University, from a Biotechnologies Engineering School or from a Biochemistry Engineering School, we are all united for this unique project and are willing to give it our all to make it succeed!
In order to do so, we united our scientific knowledge and contacted various researchers to put together our protocols. At the same time, our team has spent time and energy thinking about various non-scientific aspects of our project including how to popularize scientific knowledge to raise awareness on the possibilities of synthetic biology. We have also considered throughout the project ethical and bio-safety thematics in order to insure that our protocols are not hazardous for the environment and that any genetically engineered microorganism is controled within the lab.

For more information look at our Team Page ☛ click here


Sponsors


On behalf of the entire team, we would like to thank our sponsors for allowing us to build this project and present it at the 2015 edition of the Internationnal Genetically Engineered Machine competition! Also we would like to thank our donators!







Next Page: ☛ Problem