Difference between revisions of "Team:EPF Lausanne"

Latest revision as of 02:28, 19 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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-      <h1>EPF Lausanne<br>
-        <small>Bio LOGIC</small></h1>
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-        Single cell computing
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-  <h2>EPF-Lausanne<br>
-    <small>An orthogonal complex system in a single cell</small></h2>
-  <h3>Project Description</h3>
-  <p>
-    Logic gates are the main components of every modern digital circuit. Digital circuits work with binary values that are either 0 or 1 and we can think of this values as representing a false or true state respectively; physically 0 and 1 (false or true), are represented by 0V or 5V. Logic gates are ‘’black boxes’’ that can take many binary inputs and gives generally one binary output. For example the NOT gate takes one binary input and invert it: if the input is 0 the output will be 1, while if the input is 1 the output will be 0.  The NOT gate is one of the simplest logic gates we can imagine but many other gates with different functionalities exists. For example the AND  gate takes two inputs and gives an output: the output is true (i.e. the output value is 1) if both the inputs are true, otherwise the output is false (i.e. the output value is 0). Another common example is the OR gate, which takes two inputs and gives an output: the output is false if both inputs are false, otherwise the output is true. By combining different logic gates in sequence and in parallel (each gate having its own characteristic truth table) it is possible to create digital circuits with complex behaviours.
-  </p>
-  <p>
-    The aim of our project, is to create a general framework allowing simple design of digital circuits inside living cells, using dCas9 proteins with specific gRNAs as activators or inhibitors of gene transcription.
-  </p>
-  <p>
-   Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme which can target nearly any DNA sequence complementary to its guide RNA (gRNA). The original function of the CRISPR-Cas9 system in bacteria is to cleave foreign DNA after positive match. However, a dead version of Cas9 (dCas9) unable to cut the DNA can be used as a repressor (by preventing the binding of the RNA polymerase to promoter sequences) or as an activator (our dCas9 is fused with a a polymerase recruiting element).
-  </p>
-<p>Our project relies on a three plasmids system. The first one will produce dCas9, another will gather gates acting like a gate array and the last one will act as a linker for the gates and can represent the programm.
-</p>
-<p>This modified Cas9 will form a complex with a chosen produced gRNA (guideRNA) that will allow the complex to complementary bind to the plasmids. That way dCas9 will induce the production of other gRNAs or inhibit them. The output of a gate would then be the production or not of gRNA that will go back to the linker that will induce also a gRNA to continue the program etc…
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-              <big><big><big>Team</big></big></big><br>
-              We are a team of twelve students with a multicultural background.
-            </p>
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          </div>
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-              <big><big><big>Business</big></big></big><br>
-              We explored the possibilities of biotech and synthetic biology business.
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-              <big><big><big>Research</big></big></big><br>
-              We engineered a three-channel switch that can be controlled with the intensity of blue light.
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-              <big><big><big>Modeling</big></big></big><br>
-              We made mathematic models of the interactions behind our gene switch.
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-              <big><big><big>Cooperation</big></big></big><br>
-              We got to know other iGEM teams and developed tools for everyone to use.
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-              <big><big><big>Outreach</big></big></big><br>
-              We spread the word about iGEM and synthetic biology especially in Finland.
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-              We documented the project well.
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-            $.each($('a[ref="'+referenceName+'"]'), function(j, e2){
-                e2.setAttribute("href","#"+e.id);
-                if (e2.innerHTML == "."){
-                    e2.innerHTML = "Figure " + (index+1);
-                } else {
-                    e2.innerHTML = "figure " + (index+1);
-                }
-            });
-            $.each($('span[ref="'+referenceName+'"]'), function(j, e2){
-                e2.setAttribute("href","#"+e.id);
-                if (e2.innerHTML == "."){
-                    e2.innerHTML = "Figure " + (index+1);
-                } else {
-                    e2.innerHTML = "figure " + (index+1);
-                }
-            });
-        }
-     );
-    // Table reference script
+   </section>
-    /*
-    USAGE:
-    tag table with a tab that is connected to the table, such as:
-    <table ref="intensities" />
-    When you insert a reference in text, put a tag with a ref attribute:
-    <a tab="intensities"></a>
-    Do not put href attribute, it will be replaced with a link to the image.
-    Moreover, inner html of the anchor tag will be "table x" where x is the
-    correct table number. If span or anchor has "." as the inner html, the
-    caption will be capitalized.
-    */
-    var tables = $('table[tab], img[tab]');
-    $.each(tables, function(index, e){
-            var tableName = e.getAttribute("tab");
-            if (!e.id){
-                e.setAttribute("id","table"+(index+1));
-            }
-            $.each($('a[tab="'+tableName+'"]'), function(j, e2){
-                e2.setAttribute("href","#"+e.id);
-                if (e2.innerHTML == "."){
-                    e2.innerHTML = "Table " + (index+1);
-                } else {
-                    e2.innerHTML = "table " + (index+1);
-                }
-            });
-            $.each($('span[tab="'+tableName+'"]'), function(j, e2){
-                e2.setAttribute("href","#"+e.id);
-                if (e2.innerHTML == "."){
-                    e2.innerHTML = "Table " + (index+1);
-                } else {
-                    e2.innerHTML = "table " + (index+1);
-                }
-            });
-        }
-    );
-</script>
-</div>
-<footer>
-   <div id="logorow">
-      <div class="footerlogo">
-        <a href="http://www.epfl.ch">
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-        </a>
-      </div>
-  </div>
-  <div id="yhteystiedot">
-    <p>
-      This wiki has been designed by Aalto-Helsinki.<br>
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-</script>
-<script type="text/javascript">
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-    }
-)};
-</script>
-<script type="text/javascript">
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-      var selector = "#" + ($(this)[0].classList[1]) + "-submenu";
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-)};
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-Retrieved from "<a href="https://2015.igem.org/Team:Aalto-Helsinki">https://2015.igem.org/Team:EPF_Lausanne</a>"</div>
-      <div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div>      <!-- end content -->
-      <div class="visualClear"></div>
-    </div>
-    </div>
-<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
-<div class="visualClear"></div>
-    <div id='footer-box' class='noprint'>
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-              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>        <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>       <ul id="f-list">
-     <!-- Recentchanges is not handles well DEBUG -->
-         <li id="t-recentchanges"><a href="/Special:RecentChanges"
-          title='Recent changes'>Recent changes</a></li>
-         <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Aalto-Helsinki"
-          title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
-                         <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Aalto-Helsinki"
-                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
-                   <li id="t-specialpages"><a href="/Special:SpecialPages"
-                    title="List of all special pages [q]" accesskey="q">Special pages</a>
-                   </li>
-                 <li><a href='/Special:Preferences'>My preferences</a></li>
-             </ul>
-        </div> <!-- close footer -->
-        <div id='footer'>
-       <ul id="f-list">
-               <li id="t-print"><a href="/wiki/index.php?title=Team:Aalto-Helsinki&amp;printable=yes"
-                title="Printable version of this page [p]" accesskey="p">Printable version</a>
-               </li>
-               <li id="t-permalink"><a href="/wiki/index.php?title=Team:Aalto-Helsinki&amp;oldid=403228"
-                title="Permanent link to this revision of the page">Permanent link</a>
-               </li>
-            <li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li>
-            <li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li>
-       </ul>
-   </div> <!-- close footer -->
-    </div> <!-- close footer-box -->
-<script>if (window.runOnloadHook) runOnloadHook();</script>
-</div>
-<!-- Served in 0.163 secs. --></body>
 </html>
+{{:Team:EPF_Lausanne/Footer}}