Difference between revisions of "Team:Bordeaux/Bacteria results"
(29 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
{{:Team:Bordeaux/Template:Global}} | {{:Team:Bordeaux/Template:Global}} | ||
{{:Team:Bordeaux/Template:Menu}} | {{:Team:Bordeaux/Template:Menu}} | ||
+ | |||
<html> | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | .images{ | ||
+ | width:26%; | ||
+ | padding:0.5em; | ||
+ | } | ||
+ | .text{ | ||
+ | font-size: 11pt; | ||
+ | font-weight: 301; | ||
+ | line-height: 20pt; | ||
+ | } | ||
+ | |||
+ | .pres{ | ||
+ | width:136px; | ||
+ | background-color:white; | ||
+ | |||
+ | } | ||
+ | |||
+ | #toggle1{ | ||
+ | position: absolute; | ||
+ | top:79%; | ||
+ | left:273px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle2{ | ||
+ | position: absolute; | ||
+ | top:79%; | ||
+ | left:597px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle3{ | ||
+ | position: absolute; | ||
+ | top:79%; | ||
+ | left:927px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle4{ | ||
+ | position: absolute; | ||
+ | top:85%; | ||
+ | left:273px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle5{ | ||
+ | position: absolute; | ||
+ | top:85%; | ||
+ | left:597px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle9{ | ||
+ | position: absolute; | ||
+ | top:91%; | ||
+ | left:927px; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | #toggle10{ | ||
+ | position: absolute; | ||
+ | left:600px; | ||
+ | top:97%; | ||
+ | background-color: transparent; | ||
+ | color: black; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1/jquery.min.js"></script> | ||
+ | </head> | ||
+ | |||
+ | <script type='text/javascript'> | ||
+ | jQuery(document).ready(function() | ||
+ | { | ||
+ | // On cache la zone de texte | ||
+ | jQuery('.description').hide(); | ||
+ | |||
+ | jQuery('a#photo1').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle1').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | |||
+ | jQuery('a#photo2').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle2').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | |||
+ | |||
+ | jQuery('a#photo3').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle3').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | jQuery('a#photo4').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle4').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | jQuery('a#photo5').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle5').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | jQuery('a#photo6').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle6').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | jQuery('a#photo7').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle7').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | |||
+ | jQuery('a#photo8').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle8').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | jQuery('a#photo9').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle9').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | jQuery('a#photo10').hover(function() | ||
+ | { jQuery('.pres').hide(); | ||
+ | jQuery('#toggle10').toggle();}, | ||
+ | |||
+ | function(){ | ||
+ | jQuery('.pres').hide(); | ||
+ | }); | ||
+ | |||
+ | }); | ||
+ | </script> | ||
<section align="center"> | <section align="center"> | ||
<div class="content-wrapper"> | <div class="content-wrapper"> | ||
Line 10: | Line 178: | ||
<!-- CHOOSING MATERIAL ---------------------------------------------------------------------- --> | <!-- CHOOSING MATERIAL ---------------------------------------------------------------------- --> | ||
− | + | <h3> E. coli Results </h3> | |
<h5 align="center"><b>CHOICE OF MATERIALS</b></h5> | <h5 align="center"><b>CHOICE OF MATERIALS</b></h5> | ||
Line 123: | Line 291: | ||
− | <h5 align="center"> <b><i>E. coli</i> | + | <h5 align="center"> <b><i>E. coli</i> CURDLAN PRODUCTION </b></h5> |
<br> | <br> | ||
<p align="justify">We produced some Curdlan compared to non transformed strain of <i>DH5α</i>. We analyzed data with a student test to prove our results are significant. The theoretical concentration obtained in the Control condition corresponds to the non significant measure (background signal). | <p align="justify">We produced some Curdlan compared to non transformed strain of <i>DH5α</i>. We analyzed data with a student test to prove our results are significant. The theoretical concentration obtained in the Control condition corresponds to the non significant measure (background signal). | ||
Line 129: | Line 297: | ||
N.B. We realized these figures by doing the average of all production results in each medium.</p> | N.B. We realized these figures by doing the average of all production results in each medium.</p> | ||
<br> | <br> | ||
− | <img style=" | + | <img style="width:55vw;height:20vw"src="https://static.igem.org/mediawiki/2015/thumb/4/49/BordeauxTeam_Mix_LB_and_M63_resultsV3.png/800px-BordeauxTeam_Mix_LB_and_M63_resultsV3.png"> |
<p class="reference" align ="center"> <b> Figure 5: Quantitative analysis of purified Curdlan with aniline blue</b> </p> | <p class="reference" align ="center"> <b> Figure 5: Quantitative analysis of purified Curdlan with aniline blue</b> </p> | ||
Line 136: | Line 304: | ||
<p align="justify"> In following results, we have studied production in M63 and LB media started at the same time. </p> | <p align="justify"> In following results, we have studied production in M63 and LB media started at the same time. </p> | ||
<br> | <br> | ||
− | <img style=" | + | <img style="width:32vw;height:20vw" src="https://static.igem.org/mediawiki/2015/2/23/Bordeaux_Team_LB_and_M63_resultsV2.png"> |
<p class="reference" align ="center"> <b> Figure 6: Quantitative analysis of purified Curdlan with aniline blue</b> </p> | <p class="reference" align ="center"> <b> Figure 6: Quantitative analysis of purified Curdlan with aniline blue</b> </p> | ||
Line 148: | Line 316: | ||
<p align="justify"> Part 1. To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | <p align="justify"> Part 1. To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | ||
<br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. <b>(Fig.7)<b></p> | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. <b>(Fig.7)<b></p> | ||
− | <img style=" | + | <img style="width:45vw;height:30vw" src="https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png"> |
<p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> | <p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> | ||
<br> | <br> | ||
Line 191: | Line 359: | ||
<h6 align="center"> At the end... </h6> | <h6 align="center"> At the end... </h6> | ||
<p align="center"> <b> We have obtained 3.44g of Curdlan in one month of production and with one single gene!</p> | <p align="center"> <b> We have obtained 3.44g of Curdlan in one month of production and with one single gene!</p> | ||
− | <img style=" | + | <img style="width:30vw;height:30vw" src="https://static.igem.org/mediawiki/2015/thumb/9/98/Team_Bordeaux_CURDLAN.JPG/450px-Team_Bordeaux_CURDLAN.JPG"> |
<br> <br> | <br> <br> | ||
<h6 align="center">Perspectives</h6> | <h6 align="center">Perspectives</h6> | ||
Line 240: | Line 408: | ||
</center> | </center> | ||
<br> <br> | <br> <br> | ||
− | <p align="center"> <b> | + | <p align="center"> <b> After production of Curdlan , we tested his effect on grapevine at INRA (National Institute of Agronomic Research) . With the help of Mr Anthony Bellée, our teammate applicator Curdlan to young leafs (4/5 days),and the day after she infects the leafs with Downy Mildew (see protocol page). The tests results will be available on Monday September 23th after the wiki freeze so we will present them at our presentation in Boston. </b> </p> |
− | < | + | <a href="#" id="photo1"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-3.jpg"/></a> |
+ | <a href="#" id="photo2"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-6.jpg"/></a> | ||
+ | <a href="#" id="photo3"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-7.jpg"/></a> | ||
+ | <a href="#" id="photo4"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-8.jpg"/></a> | ||
+ | <a href="#" id="photo5"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-9.jpg"/></a> | ||
+ | <a href="#" id="photo6"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623741-10.jpg"/></a> | ||
+ | <a href="#" id="photo7"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623742-11.jpg"/></a> | ||
+ | <a href="#" id="photo8"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623742-12.jpg"/></a> | ||
+ | <a href="#" id="photo9"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623742-13.jpg"/></a> | ||
+ | <a href="#" id="photo10"><img class="images" src="http://image.noelshack.com/fichiers/2015/38/1442623743-14.jpg"/></a> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="pres" id="toggle1"> <p class="text"> <strong>Curdlan is pulverized on leafs and back to culture room during 24h</strong></p></div> | ||
+ | <div class="pres" id="toggle2"> <p class="text"><strong>We did 3 different condition ,curdlan produced by ecoli, commercial curdlan and Water </strong></p></div> | ||
+ | <div class="pres" id="toggle3"> <p class="text"><strong>Downy mildiew after 7days of development</strong></p></div> | ||
+ | <div class="pres" id="toggle4"> <p class="text"><strong>Microscopic observation of Downy Mildiew ORG </strong> </p></div> | ||
+ | <div class="pres" id="toggle5"> <p class="text"><strong>Infecting leafs with Downy mildiew</strong> </p></div> | ||
+ | <div class="pres" id="toggle6"> <p class="text"></p></div> | ||
+ | <div class="pres" id="toggle7"> <p class="text"></p></div> | ||
+ | <div class="pres" id="toggle8"> <p class="text"></p></div> | ||
+ | <div class="pres" id="toggle9"> <p class="text"><strong>Confine leafs and let them sleep during 8 days </strong></p></div> | ||
+ | <div class="pres" id="toggle10"><p class="text"><strong>Results will be available in Boston good night ...</strong></p></div> | ||
− | |||
</div> | </div> | ||
</div> | </div> | ||
Line 254: | Line 452: | ||
<h6> <a href= "https://2015.igem.org/Team:Bordeaux/AchievementsNotebook" style=" color: #FF5E00;"> Notebook & Protocols ☚ </a> Previous Page . Next Page <a href= "https://2015.igem.org/Team:Bordeaux/Yeast_results" style=" color: #FF5E00;"> ☛ Yeast Results </h6> | <h6> <a href= "https://2015.igem.org/Team:Bordeaux/AchievementsNotebook" style=" color: #FF5E00;"> Notebook & Protocols ☚ </a> Previous Page . Next Page <a href= "https://2015.igem.org/Team:Bordeaux/Yeast_results" style=" color: #FF5E00;"> ☛ Yeast Results </h6> | ||
</center> | </center> | ||
− | </div> </div> | + | </div> </div> </div> |
Latest revision as of 02:39, 19 September 2015