Difference between revisions of "Template:Heidelberg/project/standardization/onthesouthern"
(7 intermediate revisions by 2 users not shown) | |||
Line 13: | Line 13: | ||
<div class="panel‐body"> | <div class="panel‐body"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col-lg- | + | |
+ | <div class="col-lg-9"> | ||
<p class="basictext"> | <p class="basictext"> | ||
− | Not only can the HRP be used in the | + | Not only can the HRP be used in the AptaBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation<x-ref>Kershaw2012</x-ref> (Fig. 4). This system can be used to avoid working with radioactive isotopes and also poses an alternative to using fluorescent probes. |
</p> | </p> | ||
− | + | </div> | |
− | + | <div class="col-lg-3"> | |
− | + | ||
− | <div class="col-lg- | + | |
<div class="imagewrapper"> | <div class="imagewrapper"> | ||
<div class="imagewrapperheader"> | <div class="imagewrapperheader"> | ||
Line 30: | Line 29: | ||
<div class="imagewrappercaption"> | <div class="imagewrappercaption"> | ||
<strong> | <strong> | ||
− | + | Figure 4. DNA or RNA labeled with HRP-mimicking DNAzyme | |
</strong> | </strong> | ||
<p class="basictext"> | <p class="basictext"> | ||
− | + | RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation | |
</p> | </p> | ||
− | |||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 02:41, 19 September 2015
On the Southern Blot
Not only can the HRP be used in the AptaBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation
![](https://static.igem.org/mediawiki/2015/d/d7/Fig4_SplintHRP.png)
Figure 4. DNA or RNA labeled with HRP-mimicking DNAzyme
RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation