Difference between revisions of "Team:Tuebingen/Description"
Line 116: | Line 116: | ||
<g class="hover_group" opacity="0"> | <g class="hover_group" opacity="0"> | ||
− | <a xlink:href=" | + | <a xlink:href="https://2015.igem.org/Team:Tuebingen/Design?slide=3"> |
<polygon onmouseover="renderInfobox('on',1)" onmouseout="renderInfobox('off',1)" points="0,0 1000,0 1000,330 0,330" /> | <polygon onmouseover="renderInfobox('on',1)" onmouseout="renderInfobox('off',1)" points="0,0 1000,0 1000,330 0,330" /> | ||
</a> | </a> | ||
Line 122: | Line 122: | ||
<g class="hover_group" opacity="0"> | <g class="hover_group" opacity="0"> | ||
− | <a xlink:href=" | + | <a xlink:href="https://2015.igem.org/Team:Tuebingen/Design?slide=1"> |
<polygon onmouseover="renderInfobox('on',2)" onmouseout="renderInfobox('off',2)" points="0,330 1000,330 1000,540 400,540 400,700 0,700" /> | <polygon onmouseover="renderInfobox('on',2)" onmouseout="renderInfobox('off',2)" points="0,330 1000,330 1000,540 400,540 400,700 0,700" /> | ||
</a> | </a> | ||
Line 128: | Line 128: | ||
<g class="hover_group" opacity="0"> | <g class="hover_group" opacity="0"> | ||
− | <a xlink:href=" | + | <a xlink:href="https://2015.igem.org/Team:Tuebingen/Design?slide=1"> |
<polygon onmouseover="renderInfobox('on',3)" onmouseout="renderInfobox('off',3)" points="1000,330 1600,330 1600,700 400,700 400,540 1000,540" /> | <polygon onmouseover="renderInfobox('on',3)" onmouseout="renderInfobox('off',3)" points="1000,330 1600,330 1600,700 400,700 400,540 1000,540" /> | ||
</a> | </a> | ||
Line 134: | Line 134: | ||
<g class="hover_group" opacity="0"> | <g class="hover_group" opacity="0"> | ||
− | <a xlink:href=" | + | <a xlink:href="https://2015.igem.org/Team:Tuebingen/Design?slide=2"> |
<polygon onmouseover="renderInfobox('on',4)" onmouseout="renderInfobox('off',4)" points="250,700 1920,700 1920,1080 1920,1080 250,1080" /> | <polygon onmouseover="renderInfobox('on',4)" onmouseout="renderInfobox('off',4)" points="250,700 1920,700 1920,1080 1920,1080 250,1080" /> | ||
</a> | </a> |
Revision as of 02:41, 19 September 2015
![](https://static.igem.org/mediawiki/2015/b/ba/Team_Tuebingen_menu_coli_mit_lightstrahl.png)
Project Description
A Biosensor Memory Module: Cre Sensor
To achieve the construction of a reversibly activatable Cre recombinase we want to apply the caging mechanism described by Zhou et al [1]. This caging is performed by fusing a copy of a variant of the fluorescent protein Dronpa to both the C- and N-terminus of the Cre recombinase. Since this Dronpa variant is able to form monomers or dimers depending on illumination with light of different wavelengths, we hope that the dimerized form inhibits the activity of the Cre recombinase.
Because our system only needs the expression of the caged Cre construct to be dependent on a sensor, it can be combined with almost all Biosensors that include a means of transcriptional control. This gives the system a wide variety of possible applications, especially in the context of the work of other iGEM teams.
[1] Zhou, XX; Chung, HK; Lam, AJ & Lin, MZ. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814.
![](https://static.igem.org/mediawiki/2015/archive/e/eb/20150819144600%21Team_Tuebingen_Coli_chalkstyle.png)