Difference between revisions of "Team:Kent/Notebook"

Line 4: Line 4:
  
 
<html>
 
<html>
 +
 +
<style>
 +
.yui3-button {
 +
    margin:10px 0px 10px 0px;
 +
    color: #fff;
 +
    background-color: #3476b7;
 +
}
 +
</style>
 +
 +
<div id="demo" class="yui3-skin-sam yui3-g"> <!-- You need this skin class -->
 +
 +
  <div id="leftcolumn" class="yui3-u">
 +
    <!-- Container for the calendar -->
 +
    <div id="mycalendar"></div>
 +
  </div>
 +
  <div id="rightcolumn" class="yui3-u">
 +
  <div id="links" style="padding-left:20px;">
 +
      <!-- The buttons are created simply by assigning the correct CSS class -->
 +
      <button id="togglePrevMonth" class="yui3-button">Toggle Previous Month's Dates</button><br>
 +
      <button id="toggleNextMonth" class="yui3-button">Toggle Next Month's Dates</button><br>
 +
      Selected date: <span id="selecteddate"></span>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
<script type="text/javascript">
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YUI().use('calendar', 'datatype-date', 'cssbutton',  function(Y) {
 +
 +
    // Create a new instance of calendar, placing it in
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    // #mycalendar container, setting its width to 340px,
 +
    // the flags for showing previous and next month's
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    // dates in available empty cells to true, and setting
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    // the date to today's date.
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    var calendar = new Y.Calendar({
 +
      contentBox: "#mycalendar",
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      width:'340px',
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      showPrevMonth: true,
 +
      showNextMonth: true,
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      date: new Date()}).render();
 +
 +
    // Get a reference to Y.DataType.Date
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    var dtdate = Y.DataType.Date;
 +
 +
    // Listen to calendar's selectionChange event.
 +
    calendar.on("selectionChange", function (ev) {
 +
 +
      // Get the date from the list of selected
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      // dates returned with the event (since only
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      // single selection is enabled by default,
 +
      // we expect there to be only one date)
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      var newDate = ev.newSelection[0];
 +
 +
      // Format the date and output it to a DOM
 +
      // element.
 +
      Y.one("#selecteddate").setHTML(dtdate.format(newDate));
 +
    });
 +
 +
 +
    // When the 'Show Previous Month' link is clicked,
 +
    // modify the showPrevMonth property to show or hide
 +
    // previous month's dates
 +
    Y.one("#togglePrevMonth").on('click', function (ev) {
 +
      ev.preventDefault();
 +
      calendar.set('showPrevMonth', !(calendar.get("showPrevMonth")));
 +
    });
 +
 +
    // When the 'Show Next Month' link is clicked,
 +
    // modify the showNextMonth property to show or hide
 +
    // next month's dates
 +
    Y.one("#toggleNextMonth").on('click', function (ev) {
 +
      ev.preventDefault();
 +
      calendar.set('showNextMonth', !(calendar.get("showNextMonth")));
 +
    });
 +
});
 +
</script>
  
 
<style>
 
<style>

Revision as of 13:25, 9 July 2015


iGEM Kent 2015

Notebook

June

M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

July

M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 2930 31

August

M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 2627 28 29 30
31

September

M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30



















Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Meeting with advisors to discuss progress

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSB1C3

    Day 7 Wet lab 30/06/15

  • Miniprep of PSB1A3 plasmid
  • Gel electrophoresis of a large quantity of PSB1C3
  • Meeting with advisors to discuss progress

    • Day 8 Wet lab 01/07/15

  • Gel electrophoresis of the purified DNA extracted
  • Transformation of pSB1A3 circular plasmid to VS45 cells (competent)
  • Digest of all pSB1C3 plasmids

    • Day 9 Wet lab 02/07/15

  • Competent cells transformation efficiency
  • Mini-prep of 1MS340 to get pSB1C3 plasmid
  • Gel extraction of big digest
  • Quantification of the digest. Added sample buffer to each digest

    • Day 10 Wet lab 03/07/15

  • Transformation efficiency

    • Day 11 Wet lab 06/07/15

  • TOP10 cells containing pSB1A3 with limonene synthase were cultured overnight on AMP plates
  • Colonies of VS45 containing pSB1A3 were patched onto chloramphenicol plates
  • Overnight digest of miniprepped pSBIC3 using ECORI and PSTl

    • Day 12 Wet lab 07/07/15

  • An agarose gel of the overnight digest was ran, but no bands were visible
  • Set up overnight cultures of pSBIC3 and pSBIA3 to be miniprepped the next day
  • Set up an overnight digest of pSBIC3

    • Day 13 Wet lab 08/07/15

  • Agarose gel of overnight digest was ran
  • miniprep of pSBIA3 and pSBIC3

    • Day 14 Wet lab 09/07/15

    Agarose gel of digested pSBIA3 and pSBIC3