Difference between revisions of "Team:UNC-Chapel Hill"

 
(44 intermediate revisions by 4 users not shown)
Line 1: Line 1:
<html>
+
{{UNC-CSS/Main}}
<style type="text/css">
+
{{:Team:UNC-Chapel_Hill/practicemenu}}
  
.image {  
+
<html>
width: 100%;  
+
<style>
position: fixed;  
+
#colorstrip{
}  
+
    width: 100%; height: 30px;
 +
    border-style: solid;
 +
    border-color: #56A0D3;
 +
    background-color: #56A0D3;
 +
}
 +
</style>
  
h10 {
+
<div id = "colorstrip"></div>
  position: absolute;
+
   
top: 200px;
+
</html>
  width: 100%;
+
{{UNC-Chapel_Hill/carousel}}
font-size: 60px;
+
<html>
color: white;
+
padding: 5px;
+
clear: left;
+
}
+
  
h11{
+
<!-- First block of content -->
  position: absolute;
+
<div class = spacer> </spacer>
top: 300px;
+
<!-- end of first block -->
  width: 100%;
+
font-size: 60px;
+
color: white;
+
padding: 5px;
+
clear: left;
+
}
+
+
  
</style>
+
<head>
 +
<link href="https://2015.igem.org/Template:UNC-Chapel_Hill/CSS?action=raw&ctype=text/css" rel="stylesheet">
 +
</head>
  
<div class = “image”>
 
              <img src= https://static.igem.org/mediawiki/2015/7/79/Background.jpeg width = 1280px height = 800px>
 
  
<h10> University of North Carolina at </h10>
 
<h11>Chapel Hill iGEM 2015</h11>
 
 
</div>
 
</html>
 
  
{{UNC-Chapel Hill}}
 
<html>
 
  
<h2> Welcome to iGEM 2015! </h2>
+
<div id="colorstrip"></div>
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
 
 +
<!--  Block of content 1 -->
 +
<table width="100%"  cellspacing="0" height="500px">
 +
<tr><td  bgColor="#f2f7fc"></td>
 +
<td width="975px" align="center" bgColor="#f2f7fc" >
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
 
 +
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">
 +
 
 +
 
 +
<!-- Content goes here -->
 +
 
 +
 
 +
 
 +
 
 +
<h3 style="color:#56A0D3;">Background</h3>
 +
<p>
 +
Type 1 diabetes affects the production of insulin in the body, requiring continual monitoring of blood glucose levels in order to prevent the worsening of symptoms, which in many cases lead to death. With the development of solutions to produce supplemental insulin emerging, the production of insulin is no longer problematic in developed countries. However, glucose monitoring is and continues to be a source of financial trouble for many people with Type 1 diabetes. The current method for measuring blood sugar levels in diabetics utilizes a blood sugar monitor and expensive testing strips. The most basic can cost at least of $30.00 and test strips $1.38 each (One Touch Ultra)<sup>1</sup>, and when taken into account that diabetics must test their blood at least five times a day with these non reusable strips, the glucose monitoring equipment can cost at a minimum $2500 a year. In addition, insulin costs may be much higher, creating an even bigger burden.
 +
</p>
 +
</td> 
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
<!--  Block of content 2 -->
 +
<table width="100%"  cellspacing="0" height="275px">
 +
<tr>
 +
<td  bgColor="#c9def2"></td>
 +
<td width="975px" align="center" bgColor="#c9def2" >
 +
 
 +
<table  width="975px"  cellspacing="0" height="275px">
 +
<tr>
 +
<td width="95%" bgColor="#c9def2"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 
 +
<h3 style="color:#56A0D3;">Motivation and Example System</h3>
 +
<p>Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations.
 +
</p>
 +
 
 +
</td> 
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
</td>
 +
<td bgColor="#c9def2"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
<!--  Block of content 3 -->
 +
<table width="100%"  cellspacing="0" height="350px">
 +
<tr><td  bgColor="#f2f7fc"></td>
 +
<td width="975px" align="center" bgColor="#f2f7fc" >
 +
<table  width="975px"  cellspacing="0" height="350px">
 +
<tr>
 +
 
 +
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">
 +
 
 +
<!-- Content goes here -->
 +
 
 +
 
 +
<h3 style="color:#56A0D3;">Originality and Improvement</h3>
 +
<p>
 +
While glucose sensitive reporter systems are not a new to concept to iGEM, our novel three-color system revolutionizes the creation of sensing systems. This would solve the qualitative characterization flaw inherent in the current one-color systems, especially those utilizing chromoproteins. Our sensor has cut sites before and after each promoter, allowing the interchange of various promoters depending on what the user wants to measure. This customization allows for the three-color system to be applied into other fields where sensitive measurement is of vital importance.
 +
</p>
 +
</td> 
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
<!--  Block of content 4 -->
 +
<table width="100%"  cellspacing="0" height="200px">
 +
<tr>
 +
<td  bgColor="#c9def2"></td>
 +
<td width="975px" align="center" bgColor="#c9def2" >
 +
 
 +
<table  width="975px" cellspacing="0" height="200px">
 +
<tr>
 +
<td width="95%" bgColor="#c9def2"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 
 +
<h3 style="color:#56A0D3;">Project Aim</h3>
 +
<p>In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration.
 +
</p>
 +
 
 +
</td> 
 +
</table>
 +
</td>
 +
<td bgColor="#c9def2"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
  
<h4>Before you start: </h4>
+
<!--  Block of content 5 -->
<p> Please read the following pages:</p>
+
<table width="100%"  cellspacing="0" height="200px">
<ul>
+
<tr><td bgColor="#f2f7fc"></td>
<li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
+
<td width="975px" align="center" bgColor="#f2f7fc" >
<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
+
<table  width="975px"  cellspacing="0" height="200px">
</ul>
+
<tr>
  
<div class="highlightBox">
+
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">  
<h4> Styling your wiki </h4>
+
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
</div>
+
  
<h4> Editing your wiki </h4>
+
<!-- Content goes here -->
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UChicago&action=edit"> Click here to edit this page! </a></p>
+
<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
+
  
  
<h4>Templates </h4>
+
<h3 style="color:#56A0D3;">References</h3>
<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
+
<p>1. "Diabetic Test Strips : Home Diagnostic Tests - Walmart.com." Walmart.com. Walmart, n.d. Web. 22 Apr. 2015. <br />
<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
+
  
 +
2. Nazario, Brunilda. "Normal Blood Sugar Levels Chart for Adults." WebMD. WebMD, 23 Oct. 2014. Web. 20 Apr. 2015. <br />
  
<h4>Tips</h4>
+
3. http://parts.igem.org/wiki/index.php?title=Part:BBa_K118011 <br />
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
<ul>
+
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<li>Be clear about what you are doing and how you plan to do this.</li>
+
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
+
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
+
<li>Have lots of fun! </li>
+
</ul>  
+
  
 +
4. http://parts.igem.org/wiki/index.php?title=Part:BBa_K861171
 +
</p>
 +
</td> 
 +
</table>
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
  
<h4>Inspiration</h4>
 
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
<ul>
 
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
</ul>
 
  
<h4> Uploading pictures and files </h4>
+
<!-- Final block of content -->
<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
<table  width="100%" align = "center" cellspacing="0" height="200px">
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
<tr>
 +
<td width="100%" align="center" bgColor="#FFFFFF" >
 +
<h3 style="color:#56A0D3 text-align:center">Special thanks to all of our sponsers!</h3>
 +
</td>
 +
</tr>
  
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
<tr>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
 +
<img src ="https://static.igem.org/mediawiki/2015/d/de/Unc-sponsers.png">
  
 +
</td>
 +
</tr>
 +
</table>
  
 +
<!-- end of final block -->
  
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
<footer>
 +
<a href="#" class="go-top">Top</a>
  
 +
</footer>
 
</html>
 
</html>

Latest revision as of 03:01, 19 September 2015

Background

Type 1 diabetes affects the production of insulin in the body, requiring continual monitoring of blood glucose levels in order to prevent the worsening of symptoms, which in many cases lead to death. With the development of solutions to produce supplemental insulin emerging, the production of insulin is no longer problematic in developed countries. However, glucose monitoring is and continues to be a source of financial trouble for many people with Type 1 diabetes. The current method for measuring blood sugar levels in diabetics utilizes a blood sugar monitor and expensive testing strips. The most basic can cost at least of $30.00 and test strips $1.38 each (One Touch Ultra)1, and when taken into account that diabetics must test their blood at least five times a day with these non reusable strips, the glucose monitoring equipment can cost at a minimum $2500 a year. In addition, insulin costs may be much higher, creating an even bigger burden.

Motivation and Example System

Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations.

Originality and Improvement

While glucose sensitive reporter systems are not a new to concept to iGEM, our novel three-color system revolutionizes the creation of sensing systems. This would solve the qualitative characterization flaw inherent in the current one-color systems, especially those utilizing chromoproteins. Our sensor has cut sites before and after each promoter, allowing the interchange of various promoters depending on what the user wants to measure. This customization allows for the three-color system to be applied into other fields where sensitive measurement is of vital importance.

Project Aim

In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration.

References

1. "Diabetic Test Strips : Home Diagnostic Tests - Walmart.com." Walmart.com. Walmart, n.d. Web. 22 Apr. 2015.
2. Nazario, Brunilda. "Normal Blood Sugar Levels Chart for Adults." WebMD. WebMD, 23 Oct. 2014. Web. 20 Apr. 2015.
3. http://parts.igem.org/wiki/index.php?title=Part:BBa_K118011
4. http://parts.igem.org/wiki/index.php?title=Part:BBa_K861171

Special thanks to all of our sponsers!