Difference between revisions of "Team:UNC-Chapel Hill"

 
(40 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{Template:All_Teams}  
+
{{UNC-CSS/Main}}
 +
{{:Team:UNC-Chapel_Hill/practicemenu}}
 +
 
 
<html>
 
<html>
−
<head>
+
<style>
−
<title>UNC iGem</title>
+
#colorstrip{
−
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
+
    width: 100%; height: 30px;
−
<meta name="description" content="" />
+
    border-style: solid;
−
<meta name="keywords" content="" />
+
    border-color: #56A0D3;
−
<!--[if lte IE 8]><script src="js/html5shiv.js"></script><![endif]-->
+
    background-color: #56A0D3;
−
<!--<script src="js/jquery.min.js"></script>
+
}
−
<script src="js/skel.min.js"></script>
+
</style>
−
<script src="js/skel-layers.min.js"></script>
+
 
−
<script src="js/init.js"></script>-->
+
<div id = "colorstrip"></div>
−
       
+
   
−
        <script type="text/javascript" src="https://2015.igem.org/Template:UNC-Chapel_Hill/Jquery?
+
</html>
−
action=raw&ctype=text/javascript"></script>
+
{{UNC-Chapel_Hill/carousel}}
−
        <script type="text/javascript" src="https://2015.igem.org/Template:UNC-Chapel_Hill/skel?
+
<html>
−
action=raw&ctype=text/javascript"></script>
+
 
−
        <script type="text/javascript" src="https://2015.igem.org/Template:UNC-Chapel_Hill/skel-layers?
+
<!-- First block of content -->
−
action=raw&ctype=text/javascript"></script>
+
<div class = spacer> </spacer>
−
        <script type="text/javascript" src="https://2015.igem.org/Template:UNC-Chapel_Hill/init?
+
<!-- end of first block -->
−
action=raw&ctype=text/javascript"></script>
+
 
−
<noscript>
+
<head>
−
            <link rel="stylesheet" type="text/css"  
+
<link href="https://2015.igem.org/Template:UNC-Chapel_Hill/CSS?action=raw&ctype=text/css" rel="stylesheet">
−
                href="https://2015.igem.org/Template:UNC-Chapel_Hill/CSS/skel?action=raw&ctype=text/css" />
+
</head>
−
            <link rel="stylesheet" type="text/css"  
+
 
−
                href="https://2015.igem.org/Template:UNC-Chapel_Hill/CSS?action=raw&ctype=text/css" />
+
 
−
            <link rel="stylesheet" type="text/css"  
+
 
−
                href="https://2015.igem.org/Template:UNC-Chapel_Hill/CSS/style-xlarge?action=raw&ctype=text/css" />
+
 
−
</noscript>
+
<div id="colorstrip"></div>
−
</head>
+
 
−
<body id="top">
+
<!--  Block of content 1 -->
 +
<table width="100%" cellspacing="0" height="500px">
 +
<tr><td  bgColor="#f2f7fc"></td>
 +
<td width="975px" align="center" bgColor="#f2f7fc" >
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
 
 +
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">
 +
 
 +
 
 +
<!-- Content goes here -->
 +
 
 +
 
 +
 
 +
 
 +
<h3 style="color:#56A0D3;">Background</h3>
 +
<p>
 +
Type 1 diabetes affects the production of insulin in the body, requiring continual monitoring of blood glucose levels in order to prevent the worsening of symptoms, which in many cases lead to death. With the development of solutions to produce supplemental insulin emerging, the production of insulin is no longer problematic in developed countries. However, glucose monitoring is and continues to be a source of financial trouble for many people with Type 1 diabetes. The current method for measuring blood sugar levels in diabetics utilizes a blood sugar monitor and expensive testing strips. The most basic can cost at least of $30.00 and test strips $1.38 each (One Touch Ultra)<sup>1</sup>, and when taken into account that diabetics must test their blood at least five times a day with these non reusable strips, the glucose monitoring equipment can cost at a minimum $2500 a year. In addition, insulin costs may be much higher, creating an even bigger burden.
 +
</p>
 +
</td> 
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
<!-- Block of content 2 -->
 +
<table width="100%"  cellspacing="0" height="275px">
 +
<tr>
 +
<td  bgColor="#c9def2"></td>
 +
<td width="975px" align="center" bgColor="#c9def2" >
 +
 
 +
<table  width="975px"  cellspacing="0" height="275px">
 +
<tr>
 +
<td width="95%" bgColor="#c9def2"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 
 +
<h3 style="color:#56A0D3;">Motivation and Example System</h3>
 +
<p>Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations.
 +
</p>
 +
 
 +
</td>
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
</td>
 +
<td bgColor="#c9def2"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
<!--  Block of content 3 -->
 +
<table width="100%" cellspacing="0" height="350px">
 +
<tr><td  bgColor="#f2f7fc"></td>
 +
<td width="975px" align="center" bgColor="#f2f7fc" >
 +
<table  width="975px"  cellspacing="0" height="350px">
 +
<tr>
 +
 
 +
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">
 +
 
 +
<!-- Content goes here -->
 +
 
 +
 
 +
<h3 style="color:#56A0D3;">Originality and Improvement</h3>
 +
<p>
 +
While glucose sensitive reporter systems are not a new to concept to iGEM, our novel three-color system revolutionizes the creation of sensing systems. This would solve the qualitative characterization flaw inherent in the current one-color systems, especially those utilizing chromoproteins. Our sensor has cut sites before and after each promoter, allowing the interchange of various promoters depending on what the user wants to measure. This customization allows for the three-color system to be applied into other fields where sensitive measurement is of vital importance.
 +
</p>
 +
</td>
 +
 
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
<!--  Block of content 4 -->
 +
<table width="100%"  cellspacing="0" height="200px">
 +
<tr>
 +
<td  bgColor="#c9def2"></td>
 +
<td width="975px" align="center" bgColor="#c9def2" >
 +
 
 +
<table  width="975px" cellspacing="0" height="200px">
 +
<tr>
 +
<td width="95%" bgColor="#c9def2"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 
 +
<h3 style="color:#56A0D3;">Project Aim</h3>
 +
<p>In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration.
 +
</p>
 +
 
 +
</td> 
 +
</table>
 +
</td>
 +
<td bgColor="#c9def2"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
<!--  Block of content 5 -->
 +
<table width="100%" cellspacing="0" height="200px">
 +
<tr><td  bgColor="#f2f7fc"></td>
 +
<td width="975px" align="center" bgColor="#f2f7fc" >
 +
<table  width="975px" cellspacing="0" height="200px">
 +
<tr>
 +
 
 +
<td width="95%" bgColor="#f2f7fc"  rowspan="3" align="center">
 +
 
 +
<!-- Content goes here -->
 +
 
 +
 
 +
<h3 style="color:#56A0D3;">References</h3>
 +
<p>1. "Diabetic Test Strips : Home Diagnostic Tests - Walmart.com." Walmart.com. Walmart, n.d. Web. 22 Apr. 2015. <br />
 +
 
 +
2. Nazario, Brunilda. "Normal Blood Sugar Levels Chart for Adults." WebMD. WebMD, 23 Oct. 2014. Web. 20 Apr. 2015. <br />
 +
 
 +
3. http://parts.igem.org/wiki/index.php?title=Part:BBa_K118011 <br />
 +
 
 +
4. http://parts.igem.org/wiki/index.php?title=Part:BBa_K861171
 +
</p>
 +
</td> 
 +
</table>
 +
</td>
 +
<td bgColor="#f2f7fc"></td>
 +
</tr>
 +
</table>
 +
<!-- end of Block-->
 +
 
 +
 
 +
<!-- Final block of content -->
 +
<table  width="100%" align = "center" cellspacing="0" height="200px">
 +
<tr>
 +
<td width="100%" align="center" bgColor="#FFFFFF" >
 +
<h3 style="color:#56A0D3 text-align:center">Special thanks to all of our sponsers!</h3>
 +
</td>
 +
</tr>
  
−
<!-- Header -->
+
<tr>
−
<header id="header" class="skel-layers-fixed">
+
<td width="975px" align="center" bgColor="#FFFFFF" >
−
<h1><a href="#">University of North Carolina at Chapel Hill</a></h1>
+
<img src ="https://static.igem.org/mediawiki/2015/d/de/Unc-sponsers.png">
−
<nav id="nav">
+
−
<ul>
+
−
<li><a href="index.html">Home</a></li>
+
−
<li><a href="left-sidebar.html">Project</a></li>
+
−
<li><a href="right-sidebar.html">Parts</a></li>
+
−
<li><a href="notebook.html">Notebook</a></li>
+
−
<li><a href="#"> Other </a></li>
+
−
</ul>
+
−
</nav>
+
−
</header>
+
  
−
<!-- Banner -->
+
</td>
−
<section id="banner">
+
</tr>
−
<div class="inner">
+
</table>
−
<h2>UNC iGem</h2>
+
−
<p>Novel 3 color glucose sensor</p>
+
−
<ul class="actions">
+
−
<li><a href="#content" class="button big special">Sign Up</a></li>
+
−
<li><a href="#elements" class="button big alt">Learn More</a></li>
+
−
</ul>
+
−
</div>
+
−
</section>
+
  
−
<!-- One -->
+
<!-- end of final block -->
−
<section id="one" class="wrapper style1">
+
−
<header class="major">
+
−
<h2>A new approach to a nation wide diabetes epidemic</h2>
+
−
<p>Three color glucose indicator using E. Coli</p>
+
−
</header>
+
−
<div class="container">
+
−
<div class="row">
+
−
<div class="4u">
+
−
<section class="special box">
+
−
<i class="icon fa-area-chart major"></i>
+
−
<h3>Justo placerat</h3>
+
−
<p>Eu non col commodo accumsan ante mi. Commodo consectetur sed mi adipiscing accumsan ac nunc tincidunt lobortis.</p>
+
−
</section>
+
−
</div>
+
−
<div class="4u">
+
−
<section class="special box">
+
−
<i class="icon fa-refresh major"></i>
+
−
<h3>Blandit quis curae</h3>
+
−
<p>Eu non col commodo accumsan ante mi. Commodo consectetur sed mi adipiscing accumsan ac nunc tincidunt lobortis.</p>
+
−
</section>
+
−
</div>
+
−
<div class="4u">
+
−
<section class="special box">
+
−
<i class="icon fa-cog major"></i>
+
−
<h3>Amet sed accumsan</h3>
+
−
<p>Eu non col commodo accumsan ante mi. Commodo consectetur sed mi adipiscing accumsan ac nunc tincidunt lobortis.</p>
+
−
</section>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</section>
+
−
+
−
<!-- Two -->
+
−
<section id="two" class="wrapper style2">
+
−
<header class="major">
+
−
<h2>Commodo accumsan aliquam</h2>
+
−
<p>Amet nisi nunc lorem accumsan</p>
+
−
</header>
+
−
<div class="container">
+
−
<div class="row">
+
−
<div class="6u">
+
−
<section class="special">
+
−
<a href="#" class="image fit"><img src="images/pic01.jpg" alt="" /></a>
+
−
<h3>Mollis adipiscing nisl</h3>
+
−
<p>Eget mi ac magna cep lobortis faucibus accumsan enim lacinia adipiscing metus urna adipiscing cep commodo id. Ac quis arcu amet. Arcu nascetur lorem adipiscing non faucibus odio nullam arcu lobortis. Aliquet ante feugiat. Turpis aliquet ac posuere volutpat lorem arcu aliquam lorem.</p>
+
−
<ul class="actions">
+
−
<li><a href="#" class="button alt">Learn More</a></li>
+
−
</ul>
+
−
</section>
+
−
</div>
+
−
<div class="6u">
+
−
<section class="special">
+
−
<a href="#" class="image fit"><img src="images/pic02.jpg" alt="" /></a>
+
−
<h3>Neque ornare adipiscing</h3>
+
−
<p>Eget mi ac magna cep lobortis faucibus accumsan enim lacinia adipiscing metus urna adipiscing cep commodo id. Ac quis arcu amet. Arcu nascetur lorem adipiscing non faucibus odio nullam arcu lobortis. Aliquet ante feugiat. Turpis aliquet ac posuere volutpat lorem arcu aliquam lorem.</p>
+
−
<ul class="actions">
+
−
<li><a href="#" class="button alt">Learn More</a></li>
+
−
</ul>
+
−
</section>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</section>
+
  
−
<!-- Three -->
+
<footer>
−
<section id="three" class="wrapper style1">
+
<a href="#" class="go-top">Top</a>
−
<div class="container">
+
−
<div class="row">
+
−
<div class="8u">
+
−
<section>
+
−
<h2>Mollis ut adipiscing</h2>
+
−
<a href="#" class="image fit"><img src="images/pic03.jpg" alt="" /></a>
+
−
<p>Vis accumsan feugiat adipiscing nisl amet adipiscing accumsan blandit accumsan sapien blandit ac amet faucibus aliquet placerat commodo. Interdum ante aliquet commodo accumsan vis phasellus adipiscing. Ornare a in lacinia. Vestibulum accumsan ac metus massa tempor. Accumsan in lacinia ornare massa amet. Ac interdum ac non praesent. Cubilia lacinia interdum massa faucibus blandit nullam. Accumsan phasellus nunc integer. Accumsan euismod nunc adipiscing lacinia erat ut sit. Arcu amet. Id massa aliquet arcu accumsan lorem amet accumsan commodo odio cubilia ac eu interdum placerat placerat arcu commodo lobortis adipiscing semper ornare pellentesque.</p>
+
−
</section>
+
−
</div>
+
−
<div class="4u">
+
−
<section>
+
−
<h3>Magna massa blandit</h3>
+
−
<p>Feugiat amet accumsan ante aliquet feugiat accumsan. Ante blandit accumsan eu amet tortor non lorem felis semper. Interdum adipiscing orci feugiat penatibus adipiscing col cubilia lorem ipsum dolor sit amet feugiat consequat.</p>
+
−
<ul class="actions">
+
−
<li><a href="#" class="button alt">Learn More</a></li>
+
−
</ul>
+
−
</section>
+
−
<hr />
+
−
<section>
+
−
<h3>Ante sed commodo</h3>
+
−
<ul class="alt">
+
−
<li><a href="#">Erat blandit risus vis adipiscing</a></li>
+
−
<li><a href="#">Tempus ultricies faucibus amet</a></li>
+
−
<li><a href="#">Arcu commodo non adipiscing quis</a></li>
+
−
<li><a href="#">Accumsan vis lacinia semper</a></li>
+
−
</ul>
+
−
</section>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</section>
+
−
+
−
<!-- Footer -->
+
−
<footer id="footer">
+
−
<div class="container">
+
−
<div class="row double">
+
−
<div class="6u">
+
−
<div class="row collapse-at-2">
+
−
<div class="6u">
+
−
<h3>Accumsan</h3>
+
−
<ul class="alt">
+
−
<li><a href="#">Nascetur nunc varius</a></li>
+
−
<li><a href="#">Vis faucibus sed tempor</a></li>
+
−
<li><a href="#">Massa amet lobortis vel</a></li>
+
−
<li><a href="#">Nascetur nunc varius</a></li>
+
−
</ul>
+
−
</div>
+
−
<div class="6u">
+
−
<h3>Faucibus</h3>
+
−
<ul class="alt">
+
−
<li><a href="#">Nascetur nunc varius</a></li>
+
−
<li><a href="#">Vis faucibus sed tempor</a></li>
+
−
<li><a href="#">Massa amet lobortis vel</a></li>
+
−
<li><a href="#">Nascetur nunc varius</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<div class="6u">
+
−
<h2>Aliquam Interdum</h2>
+
−
<p>Blandit nunc tempor lobortis nunc non. Mi accumsan. Justo aliquet massa adipiscing cubilia eu accumsan id. Arcu accumsan faucibus vis ultricies adipiscing ornare ut. Mi accumsan justo aliquet.</p>
+
−
<ul class="icons">
+
−
<li><a href="#" class="icon fa-twitter"><span class="label">Twitter</span></a></li>
+
−
<li><a href="#" class="icon fa-facebook"><span class="label">Facebook</span></a></li>
+
−
<li><a href="#" class="icon fa-instagram"><span class="label">Instagram</span></a></li>
+
−
<li><a href="#" class="icon fa-linkedin"><span class="label">LinkedIn</span></a></li>
+
−
<li><a href="#" class="icon fa-pinterest"><span class="label">Pinterest</span></a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
<ul class="copyright">
+
−
<li>&copy; Untitled. All rights reserved.</li>
+
−
<li>Design: <a href="http://templated.co">TEMPLATED</a></li>
+
−
<li>Images: <a href="http://unsplash.com">Unsplash</a></li>
+
−
</ul>
+
−
</div>
+
−
</footer>
+
  
−
</body>
+
</footer>
 
</html>
 
</html>

Latest revision as of 03:01, 19 September 2015


Background

Type 1 diabetes affects the production of insulin in the body, requiring continual monitoring of blood glucose levels in order to prevent the worsening of symptoms, which in many cases lead to death. With the development of solutions to produce supplemental insulin emerging, the production of insulin is no longer problematic in developed countries. However, glucose monitoring is and continues to be a source of financial trouble for many people with Type 1 diabetes. The current method for measuring blood sugar levels in diabetics utilizes a blood sugar monitor and expensive testing strips. The most basic can cost at least of $30.00 and test strips $1.38 each (One Touch Ultra)1, and when taken into account that diabetics must test their blood at least five times a day with these non reusable strips, the glucose monitoring equipment can cost at a minimum $2500 a year. In addition, insulin costs may be much higher, creating an even bigger burden.

Motivation and Example System

Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations.

Originality and Improvement

While glucose sensitive reporter systems are not a new to concept to iGEM, our novel three-color system revolutionizes the creation of sensing systems. This would solve the qualitative characterization flaw inherent in the current one-color systems, especially those utilizing chromoproteins. Our sensor has cut sites before and after each promoter, allowing the interchange of various promoters depending on what the user wants to measure. This customization allows for the three-color system to be applied into other fields where sensitive measurement is of vital importance.

Project Aim

In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration.

References

1. "Diabetic Test Strips : Home Diagnostic Tests - Walmart.com." Walmart.com. Walmart, n.d. Web. 22 Apr. 2015.
2. Nazario, Brunilda. "Normal Blood Sugar Levels Chart for Adults." WebMD. WebMD, 23 Oct. 2014. Web. 20 Apr. 2015.
3. http://parts.igem.org/wiki/index.php?title=Part:BBa_K118011
4. http://parts.igem.org/wiki/index.php?title=Part:BBa_K861171

Special thanks to all of our sponsers!