Difference between revisions of "Team:WHU-China/Parts"

 
(20 intermediate revisions by 3 users not shown)
Line 95: Line 95:
 
 
 
.secmenu ul li{
 
.secmenu ul li{
 +
                margin-top:15px;
 
height:40px;
 
height:40px;
 
width:150px;
 
width:150px;
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.secmenu ul li a{
 
.secmenu ul li a{
 
line-height:20px;
 
line-height:20px;
−
font-family: 'Lato', sans-serif;
+
font-family: Helvetica,Arial,Akzidenz-Grotesk,Univers,Frutiger;
 
font-size:18px;
 
font-size:18px;
 
color:#000;
 
color:#000;
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font-family:Tahoma, Geneva, sans-serif;
 
font-family:Tahoma, Geneva, sans-serif;
 
font-size:18px;
 
font-size:18px;
 +
}
 +
#next{
 +
position:absolute;
 +
left:40px;
 +
top:540px;
 +
z-index:100;
 +
-webkit-transition: all 0.3s ease;
 +
-moz-transition: all 0.3s ease;
 +
-o-transition: all 0.3s ease;
 +
transition: all 0.3s ease;
 
}
 
}
 
</style>  
 
</style>  
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window.onload=myfun;
 
window.onload=myfun;
 
function next_member(){
 
function next_member(){
−
if(num_member==3){
+
if(num_member==2){
 
alert("the last page");
 
alert("the last page");
 
return ;
 
return ;
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     <div class="left">
 
     <div class="left">
 
     </div>
 
     </div>
 +
    <img src="https://static.igem.org/mediawiki/2015/d/d1/WHU-China_next.png" id="next" />
 
<div class="container">
 
<div class="container">
 
     <img src="https://static.igem.org/mediawiki/2015/e/ea/WHU-China_4.png" />
 
     <img src="https://static.igem.org/mediawiki/2015/e/ea/WHU-China_4.png" />
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         </div>
 
         </div>
 
         <!--header end-->
 
         <!--header end-->
−
         <div class="middle-left">
+
          
−
        <img src="https://static.igem.org/mediawiki/2015/2/20/WHU-China_3.png" />
+
−
            <img src="https://static.igem.org/mediawiki/2015/3/34/WHU-China_10.png" id="whu-logo"/>
+
−
            <div class="secmenu" id="sec">
+
−
                <ul id="tit1">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Team">Member</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Attributions">Attributions</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Collaborations">Collaborations</a></li>
+
−
                </ul>
+
−
                <ul id="tit2">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Description?num=1">Overview</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Description?num=2">Criticality Detector</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Description?num=6">Binary System</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Description?num=8">Dosage Control System</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Result">Results</a></li>
+
−
                </ul>
+
−
                <ul id="tit3">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Parts">Team Parts</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Basic_Parts">Basis Parts</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Composite_Part">Composite Parts</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/F_Part">Favorite Parts</a></li>
+
−
                </ul>
+
−
                <ul id="tit4">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Notebook">Notebook</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Safety">Safety</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Experiments">Protocols</a></li>
+
−
                </ul>
+
−
                <ul id="tit5">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Practices?num=5">Meet ups</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Practices?num=1">Sketch in dishes</a></li>
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Practices?num=8">Smile mates</a></li>
+
−
                </ul>
+
−
                <ul id="tit6">
+
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Modeling">Modeling</a></li>
+
−
                </ul>
+
−
            </div>
+
−
        </div>
+
−
        <!--header end-->
+
 
         <div class="middle-left">
 
         <div class="middle-left">
 
         <img src="https://static.igem.org/mediawiki/2015/2/20/WHU-China_3.png" />
 
         <img src="https://static.igem.org/mediawiki/2015/2/20/WHU-China_3.png" />
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                 <ul id="tit3">
 
                 <ul id="tit3">
 
                     <li><a href="https://2015.igem.org/Team:WHU-China/Parts">Team Parts</a></li>
 
                     <li><a href="https://2015.igem.org/Team:WHU-China/Parts">Team Parts</a></li>
−
                     <li><a href="https://2015.igem.org/Team:WHU-China/Basic_Parts">Basis Parts</a></li>
+
                     <li><a href="https://2015.igem.org/Team:WHU-China/Basic_Parts">Basic Parts</a></li>
−
                    <li><a href="https://2015.igem.org/Team:WHU-China/Composite_Part">Composite Parts</a></li>
+
−
                    <li><a href="javascript:return false;">Favorite Parts</a></li>
+
 
                 </ul>
 
                 </ul>
 
                 <ul id="tit4">
 
                 <ul id="tit4">
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         <div class="textarea">
 
         <div class="textarea">
 
         <div class="editarea" id="member1">
 
         <div class="editarea" id="member1">
−
             <h2>Team Parts</h2>
+
             <h2 style="display:inline;float:left">Team Parts</h2>
 +
                <h2 style="display:inline;float:right">Page 1 of 2</h2>
 
                 <div class="member">
 
                 <div class="member">
 
                 <span>Our favorite parts:</span>
 
                 <span>Our favorite parts:</span>
 
                 <img src="https://static.igem.org/mediawiki/2015/f/f5/WHU-China_parts1.png" height="100">
 
                 <img src="https://static.igem.org/mediawiki/2015/f/f5/WHU-China_parts1.png" height="100">
 
                 <span style="margin-top:105px" >Figure: Criticality part</span>
 
                 <span style="margin-top:105px" >Figure: Criticality part</span>
−
                 <span style="margin-top:10px">This part function as the output of light sensing.It is composed of three independent parts which are leaded by three promoter seperately, the three parts are CI operator site,CI protein under riboregulator,and taRNA.When the promoter pOmpc is activated (usually when red light turns off),there three sequences express at the same time : the CI operator site, the riboregulator, and the taRNA. First,because of the riboregulator , the CI repressor can not be produced.But then, when taRNA is expressed integrally, it works to unlock the riboregulator, so the CI protein starts to be produced. Last, the CI protein bind to the CI operator site ,and exact its function,so the following sequence is depressed,including itselve’s sequence.To sumrize the function of this part, it make the expression of CI repressor limited to a relatively short period of time:at first it can’t be produced,once it can be expressed and exact its function,it’s inhibited by itself.</span>
+
                 <span style="margin-top:10px">This part function as the output of light sensing. It is composed of three independent parts which are leaded by three promoter seperately, the three parts are CI operator site, CI protein after riboregulator, and taRNA. When the promoter PompC is activated (usually when red light turns off), these three sequences express at the same time: the CI operator site, the riboregulator, and the taRNA. First, because of the riboregulator, the CI repressor can not be produced. But then, when taRNA is expressed integrally, it works to unlock the riboregulator, so the CI protein starts to be produced. Last, the CI protein bind to the CI operator site, and exact its function, so the following sequence is depressed, including its own sequence. To summarize the function of this part, it makes the expression of CI repressor limited to a relatively short period of time: at first it can't be produced, once it can be expressed and exact its function, it's inhibited by itself.</span>
 
                 <img src="https://static.igem.org/mediawiki/2015/a/af/WHU-China_parts2.png" height="100">
 
                 <img src="https://static.igem.org/mediawiki/2015/a/af/WHU-China_parts2.png" height="100">
 
                 <span style="margin-top:105px" >Figure: Light sensitive part</span>
 
                 <span style="margin-top:105px" >Figure: Light sensitive part</span>
 
                 <span>This part is built for light sensing. It can regulate the promoter PompC, thus used for signal input in our project. It encodes three proteins, Cph8, Ho1 and PcyA. Cph8 is a hybrid photoreceptor, composed of photoreceptor Cph1 and the EnvZ histidine kinase. Ho1 and PcyA are enzymes which catalyze heme to phycocyanobilin (PCB). Cph8 requires covalently associated PCB to function. PompC is blocked when Cph8 is exposed under red light and is actived under far-red light.  
 
                 <span>This part is built for light sensing. It can regulate the promoter PompC, thus used for signal input in our project. It encodes three proteins, Cph8, Ho1 and PcyA. Cph8 is a hybrid photoreceptor, composed of photoreceptor Cph1 and the EnvZ histidine kinase. Ho1 and PcyA are enzymes which catalyze heme to phycocyanobilin (PCB). Cph8 requires covalently associated PCB to function. PompC is blocked when Cph8 is exposed under red light and is actived under far-red light.  
 
                 </span>
 
                 </span>
 +
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
 
         <div class="editarea" id="member2">
 
         <div class="editarea" id="member2">
−
        <h2>Team Parts</h2>
+
            <h2 style="display:inline;float:left">Team Parts</h2>
 +
                <h2 style="display:inline;float:right">Page 2 of 2</h2>
 
             <div class="member">
 
             <div class="member">
 
             <center><h2>Part Submission</h2></center>
 
             <center><h2>Part Submission</h2></center>
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                 <center>
 
                 <center>
 
                 <style>
 
                 <style>
 +
                                table {
 +
                                      font-family:cursive,"Times New Roman",Georgia,Serif;
 +
                                }
 
table tr td{
 
table tr td{
 +
                                       
 
max-width:300px;}
 
max-width:300px;}
 
</style>
 
</style>
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                     <td><a href="http://parts.igem.org/Part:BBa_K1647000">BBa_K1647000</a></td>
 
                     <td><a href="http://parts.igem.org/Part:BBa_K1647000">BBa_K1647000</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>C1 operator site with GFP</td>
+
                     <td>CI operator site with GFP</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>933bp</td>
 
                     <td>933bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647001</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647001">BBa_K1647001</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Pompc+Cl operon+RBS+GFP+terminator</td>
+
                     <td>PompC+Cl operon+RBS+GFP+terminator</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>1046bp</td>
 
                     <td>1046bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647002</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647002">BBa_K1647002</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
 
                     <td>B0034+ho1+B0034+pcyA+B0010</td>
 
                     <td>B0034+ho1+B0034+pcyA+B0010</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647003</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647003">BBa_K1647003</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
 
                     <td>B0034+pcyA</td>
 
                     <td>B0034+pcyA</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647004</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647004">BBa_K1647004</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
 
                     <td>B0034+ho1+B0034+pcyA</td>
 
                     <td>B0034+ho1+B0034+pcyA</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647005</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647005">BBa_K1647005</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
 
                     <td>J23100+Cph8</td>
 
                     <td>J23100+Cph8</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647006</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647006">BBa_K1647006</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>J23100+RBS+Cph8+RBS<br>
+
                     <td>J23100+B0034+Cph8+B0034<br>
−
+ho1+RBS+pcyA+B0010</td>
+
+ho1+B0034+pcyA+B0010</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>3694bp</td>
 
                     <td>3694bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647007</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647007">BBa_K1647007</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Pompc+Riborugulator</td>
+
                     <td>PompC+Riborugulator</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>156bp</td>
 
                     <td>156bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647008</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647008">BBa_K1647008</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
 
                     <td>J23100+Riborugulator+GFP</td>
 
                     <td>J23100+Riborugulator+GFP</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647009</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647009">BBa_K1647009</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Pompc+Riboregulator+C1<br>
+
                     <td>PompC+Riboregulator+CI<br>
−
+B0010+Pompc+taRNA+B0010</td>
+
+B0010+PompC+taRNA+B0010</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>1148bp</td>
 
                     <td>1148bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647010</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647010">BBa_K1647010</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
−
                     <td>C1+B0010</td>
+
                     <td>CI+B0010</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>782bp</td>
 
                     <td>782bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647011</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647011">BBa_K1647011</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
 
                     <td>Lac promoter+taRNA</td>
 
                     <td>Lac promoter+taRNA</td>
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                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647012</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647012">BBa_K1647012</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Pompc+taRNA+B0010</td>
+
                     <td>PompC+taRNA+B0010</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>298bp</td>
 
                     <td>298bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647013</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647013">BBa_K1647013</a></td>
 
                     <td>Intermediate</td>
 
                     <td>Intermediate</td>
 
                     <td>J23100+taRNA</td>
 
                     <td>J23100+taRNA</td>
Line 572: Line 552:
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647014</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647014">BBa_K1647014</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Lac promoter+C1+B0010</td>
+
                     <td>Lac promoter+CI+B0010</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>988bp</td>
 
                     <td>988bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647015</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647015">BBa_K1647015</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
 
                     <td>Lac promoter+taRNA+B0010</td>
 
                     <td>Lac promoter+taRNA+B0010</td>
Line 586: Line 566:
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647016</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647016">BBa_K1647016</a></td>
 
                     <td>Coding</td>
 
                     <td>Coding</td>
 
                     <td>CAP is a short peptide derived from the competence stimulating peptide</td>
 
                     <td>CAP is a short peptide derived from the competence stimulating peptide</td>
Line 593: Line 573:
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647017</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647017">BBa_K1647017</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Cap-app2</td>
+
                     <td>CAP-AAP2</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>82bp</td>
 
                     <td>82bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647018</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647018">BBa_K1647018</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>CAP-Bac8c, a improved antimicrobial peptide selectively</td>
+
                     <td>CAP-Bac8c, an improved antimicrobial peptide selectively</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>64bp</td>
 
                     <td>64bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647019</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647019">BBa_K1647019</a></td>
 
                     <td>Composite</td>
 
                     <td>Composite</td>
−
                     <td>Cap-app2, a improved antimicrobial peptide which can kill the S.mutans selectively and have changeab</td>
+
                     <td>Cap-AAP2, an improved antimicrobial peptide which can kill the S.mutans selectively and have changeab</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>82bp</td>
 
                     <td>82bp</td>
 
                     </tr>
 
                     </tr>
 
                     <tr>
 
                     <tr>
−
                     <td>BBa_K1647020</td>
+
                     <td><a href="http://parts.igem.org/Part:BBa_K1647020">BBa_K1647020</a></td>
 
                     <td>coding</td>
 
                     <td>coding</td>
−
                     <td>APP2, a kind of antimicrobial peptides</td>
+
                     <td>AAP2, a kind of antimicrobial peptide</td>
 
                     <td>Yun Xue</td>
 
                     <td>Yun Xue</td>
 
                     <td>48bp</td>
 
                     <td>48bp</td>
Line 624: Line 604:
 
             </div>
 
             </div>
 
         </div>
 
         </div>
−
        <div class="editarea" id="member3">
+
 
−
        <h2>Team Parts</h2>
+
−
            <div class="member">
+
−
            <span>
+
−
            <ul>
+
−
            <li>BBa_K1647000:<a href="http://parts.igem.org/Part:BBa_K1647000">http://parts.igem.org/Part:BBa_K1647000</a></li>
+
−
            <li>BBa_K1647001:<a href="http://parts.igem.org/Part:BBa_K1647001">http://parts.igem.org/Part:BBa_K1647001</a></li>
+
−
            <li>BBa_K1647002:<a href="http://parts.igem.org/Part:BBa_K1647002">http://parts.igem.org/Part:BBa_K1647002</a></li>
+
−
            <li>BBa_K1647003:<a href="http://parts.igem.org/Part:BBa_K1647003">http://parts.igem.org/Part:BBa_K1647003</a></li>
+
−
            <li>BBa_K1647004:<a href="http://parts.igem.org/Part:BBa_K1647004">http://parts.igem.org/Part:BBa_K1647004</a></li>
+
−
            <li>BBa_K1647005:<a href="http://parts.igem.org/Part:BBa_K1647005">http://parts.igem.org/Part:BBa_K1647005</a></li>
+
−
            <li>BBa_K1647006:<a href="http://parts.igem.org/Part:BBa_K1647006">http://parts.igem.org/Part:BBa_K1647006</a></li>
+
−
            <li>BBa_K1647007:<a href="http://parts.igem.org/Part:BBa_K1647007">http://parts.igem.org/Part:BBa_K1647007</a></li>
+
−
            <li>BBa_K1647008:<a href="http://parts.igem.org/Part:BBa_K1647008">http://parts.igem.org/Part:BBa_K1647008</a></li>
+
−
            <li>BBa_K1647009:<a href="http://parts.igem.org/Part:BBa_K1647009">http://parts.igem.org/Part:BBa_K1647009</a></li>
+
−
            <li>BBa_K1647010:<a href="http://parts.igem.org/Part:BBa_K1647010">http://parts.igem.org/Part:BBa_K1647010</a></li>
+
−
            <li>BBa_K1647011:<a href="http://parts.igem.org/Part:BBa_K1647011">http://parts.igem.org/Part:BBa_K1647011</a></li>
+
−
            <li>BBa_K1647012:<a href="http://parts.igem.org/Part:BBa_K1647012">http://parts.igem.org/Part:BBa_K1647012</a></li>
+
−
            <li>BBa_K1647013:<a href="http://parts.igem.org/Part:BBa_K1647013">http://parts.igem.org/Part:BBa_K1647013</a></li>
+
−
            <li>BBa_K1647014:<a href="http://parts.igem.org/Part:BBa_K1647014">http://parts.igem.org/Part:BBa_K1647014</a></li>
+
−
            <li>BBa_K1647015:<a href="http://parts.igem.org/Part:BBa_K1647015">http://parts.igem.org/Part:BBa_K1647015</a></li>
+
−
            <li>BBa_K1647016:<a href="http://parts.igem.org/Part:BBa_K1647016">http://parts.igem.org/Part:BBa_K1647016</a></li>
+
−
            <li>BBa_K1647017:<a href="http://parts.igem.org/Part:BBa_K1647017">http://parts.igem.org/Part:BBa_K1647017</a></li>
+
−
            <li>BBa_K1647018:<a href="http://parts.igem.org/Part:BBa_K1647018">http://parts.igem.org/Part:BBa_K1647018</a></li>
+
−
            <li>BBa_K1647019:<a href="http://parts.igem.org/Part:BBa_K1647019">http://parts.igem.org/Part:BBa_K1647019</a></li>
+
−
            <li>BBa_K1647020:<a href="http://parts.igem.org/Part:BBa_K1647020">http://parts.igem.org/Part:BBa_K1647020</a></li>
+
−
            <li>BBa_K1647021:<a href="http://parts.igem.org/Part:BBa_K1647021">http://parts.igem.org/Part:BBa_K1647021</a></li>
+
−
            </ul>
+
−
            </span>
+
−
            </div>
+
−
        </div>
+
 
         </div>
 
         </div>
 
         </div>
 
         </div>

Latest revision as of 03:08, 19 September 2015

圆形 圆形

Team Parts

Page 1 of 2

Our favorite parts: Figure: Criticality part This part function as the output of light sensing. It is composed of three independent parts which are leaded by three promoter seperately, the three parts are CI operator site, CI protein after riboregulator, and taRNA. When the promoter PompC is activated (usually when red light turns off), these three sequences express at the same time: the CI operator site, the riboregulator, and the taRNA. First, because of the riboregulator, the CI repressor can not be produced. But then, when taRNA is expressed integrally, it works to unlock the riboregulator, so the CI protein starts to be produced. Last, the CI protein bind to the CI operator site, and exact its function, so the following sequence is depressed, including its own sequence. To summarize the function of this part, it makes the expression of CI repressor limited to a relatively short period of time: at first it can't be produced, once it can be expressed and exact its function, it's inhibited by itself. Figure: Light sensitive part This part is built for light sensing. It can regulate the promoter PompC, thus used for signal input in our project. It encodes three proteins, Cph8, Ho1 and PcyA. Cph8 is a hybrid photoreceptor, composed of photoreceptor Cph1 and the EnvZ histidine kinase. Ho1 and PcyA are enzymes which catalyze heme to phycocyanobilin (PCB). Cph8 requires covalently associated PCB to function. PompC is blocked when Cph8 is exposed under red light and is actived under far-red light.

Team Parts

Page 2 of 2

Part Submission

Here are the parts we have submitted
Name Type Description Designer Length
BBa_K1647000 Composite CI operator site with GFP Yun Xue 933bp
BBa_K1647001 Composite PompC+Cl operon+RBS+GFP+terminator Yun Xue 1046bp
BBa_K1647002 Intermediate B0034+ho1+B0034+pcyA+B0010 Yun Xue 1658bp
BBa_K1647003 Intermediate B0034+pcyA Yun Xue 793bp
BBa_K1647004 Intermediate B0034+ho1+B0034+pcyA Yun Xue 1570bp
BBa_K1647005 Composite J23100+Cph8 Yun Xue 2298bp
BBa_K1647006 Composite J23100+B0034+Cph8+B0034
+ho1+B0034+pcyA+B0010		 Yun Xue 3694bp
BBa_K1647007 Composite PompC+Riborugulator Yun Xue 156bp
BBa_K1647008 Composite J23100+Riborugulator+GFP Yun Xue 967bp
BBa_K1647009 Composite PompC+Riboregulator+CI
+B0010+PompC+taRNA+B0010		 Yun Xue 1148bp
BBa_K1647010 Intermediate CI+B0010 Yun Xue 782bp
BBa_K1647011 Intermediate Lac promoter+taRNA Yun Xue 302bp
BBa_K1647012 Composite PompC+taRNA+B0010 Yun Xue 298bp
BBa_K1647013 Intermediate J23100+taRNA Yun Xue 137bp
BBa_K1647014 Composite Lac promoter+CI+B0010 Yun Xue 988bp
BBa_K1647015 Composite Lac promoter+taRNA+B0010 Yun Xue 390bp
BBa_K1647016 Coding CAP is a short peptide derived from the competence stimulating peptide Yun Xue 28bp
BBa_K1647017 Composite CAP-AAP2 Yun Xue 82bp
BBa_K1647018 Composite CAP-Bac8c, an improved antimicrobial peptide selectively Yun Xue 64bp
BBa_K1647019 Composite Cap-AAP2, an improved antimicrobial peptide which can kill the S.mutans selectively and have changeab Yun Xue 82bp
BBa_K1647020 coding AAP2, a kind of antimicrobial peptide Yun Xue 48bp