Difference between revisions of "Team:NTU-Singapore/Design"

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<h2>Design</h2>
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    <title>NTU SG iGEM 2015</title>
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By talking about your design work on this page, there is one medal criterion that you can attempt to meet, and one award that you can apply for. If your team is going for a gold medal by building a functional prototype, you should tell us what you did on this page. If you are going for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Applied Design award</a>, you should also complete this page and tell us what you did.  
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<h1 style="border-bottom: 0px; margin-top: -10px;">NTU S'PORE</h1>
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</a>
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<li><a href="https://2015.igem.org/Team:NTU-Singapore/Team">Team</a></li>
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                                <li><a href="https://2015.igem.org/Team:NTU-Singapore/Attributions">Attributions</a></li>
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<li><a href="https://2015.igem.org/Team:NTU-Singapore/Project">Project</a></li>
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<li><a href="https://2015.igem.org/Team:NTU-Singapore/Parts">Parts</a></li>
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<li><a href="https://2015.igem.org/Team:NTU-Singapore/Modeling">Modeling</a></li>
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<li><a href="#a1">The Concept</a></li>
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<li><a href="#a2">Design</a></li>
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<li><a href="#a3">Testing it out</a></li>
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<li><a href="#a4">Results</a></li>
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                                <li><a href="https://2015.igem.org/Team:NTU-Singapore/Experiments" style="background-color: orange; margin-bottom:-2px;">Protocols</a></li>
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                                <li><a href="https://2015.igem.org/Team:NTU-Singapore/Description" style="background-color: orange; margin-bottom:-2px;">Description</a></li>
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                                <li><a href="https://2015.igem.org/Team:NTU-Singapore/Results" style="background-color: orange; margin-bottom:-2px;">Results</a></li>
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                                <li><a href="https://2015.igem.org/Team:NTU-Singapore/Design" style="background-color: orange; margin-bottom:-2px;">Design</a></li>
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<!-- Section: Human Practice -->
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<!--Header: Human Practice-->
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<section id="about" class="home-section-humanprac text-center bg-gray pageupShezensor" >
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<br><br><br>
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<div class="heading-about" >
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<div class="container" style=" margin-top: 200px; ">
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<div class="row">
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<div class="col-lg-8 col-lg-offset-2">
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<div class="wow bounceInDown" data-wow-delay="0.4s">
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<div class="section-heading" style="margin-bottom 3px">
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<h2 style="border-bottom: 0px; color: white;">Shezensor 1.0</h2>
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<i class="fa fa-leaf fa-2x" style="color: white;"></i>
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</div>
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<div class="container" >
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<div class="inner" >
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<h5>The Concept</h5><br>
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<p class="subtitle explain"> As MFCs have the potential to be used as a biosensor which is able to detect various chemicals and produce an electrical output. The ability to convert chemical to electrical signals gives MFCs an advantage over biosensors which rely on chemical outputs, i.e. fluorescence proteins as the electrical output allows on-field monitoring, automated data collection as well as WIFI communication. <br>For our project, our idea to make a portable device which allows users to test the cleanliness of water samples. Our device has two important components, the reader and the sensor. The reader is an arduino-controlled voltmeter and has a 16x2 LCD screen which provides a voltage reading to the user. While the sensor is a disposable cartridge which houses the MFC. Liquid samples can be loaded into the sensors and plugged into the reader to check if the voltage reading. After designing the cartridge, we carried out a proof-of-concept experiment to test the feasibility of our concept by first testing whether our disposable cartridge works. <div align="centre"><img class="" src="https://static.igem.org/mediawiki/2015/c/c2/Shezensor.png" width="1100px" height="350px"></div>
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                                                </p>
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        </div>
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</div>
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<br>
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</div>
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</section>
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<!--Activity2-->
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<section id="a2" class="home-section-humanprac text-center" width="1300px">
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<div class="container" style=" width: 1200px; ">
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<div class="row boxed-grey-humanprac"  >
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<div class="wow bounceInUp" data-wow-delay="0.2s">
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<div class="inner">
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<h5>Design</h5>
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<p class="subtitle explain">As mentioned in the concept, we will be using the Arduino board as the voltage reader, hence, the programming for the Arduino board has to be done so that it can be used along with a LCD display screen. On the reader, a cartridge port was made to allow connection of the anode and cathode to the reader.<br><br> For the cartridge, the materials we used were acrylic blocks and Booby helped carve the block outs as the machines are highly dangerous. The internal dimensions are 60 X 20 X 5mm, while the dimensions of our carbon cloth is 50 X 20mm. The area allowed for the proton exchange membrane is maximised to increased voltage output, with an area of 60mm<sup>2</sup>. When all the parts are carved out with the help of wonderful Bobby, we glued them together with a specialised acrylic glue and made sure that no leakage happens.<br><br>P.S. Our first prototype leaked, and we made another one. :P
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</p>
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<div align="center">
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/3/3a/Comp_cell.png" width="605px" height="440px">
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</div>
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<p style="text-align:center; font-size: 19px; color: black">
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The Blueprint.
 
</p>
 
</p>
  
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<div class="highlightBox">
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<div align="center">
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<h4>Note</h4>
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/2/23/Building_cat.png" width="550px" height="400px" style=" margin-right: 20px; "><img class="goleft" src="https://static.igem.org/mediawiki/2015/f/fa/ShezCatridge.png" width="400px" height="400px">
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Applied Design award</a> and/or the <a href="https://2015.igem.org/Judging/Awards#Medals">functional prototype gold medal criterion</a>, you must fill out this page.</p>
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</div>
 
</div>
  
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<p>This is a prize for the team that has developed a synthetic biology product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.</p>
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<p style="text-align:center; font-size: 19px; color: black">
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The Catridge.
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</p>
  
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<p>
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<div align="center">
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If you are working on art and design as your main project, please join the art and design track. If you are integrating art and design into the core of your main project, please apply for the award by completing this page.
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/0/07/Read.png" width="720px" height="550px">
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</div>
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<p style="text-align:center; font-size: 19px; color: black">
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The Reader.
 
</p>
 
</p>
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</div>
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</div>
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</div>
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<br>
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</div>
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</div>
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</section>
  
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        <!--Activity3-->
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<section id="a3" class="home-section-humanprac text-center">
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<div class="container">
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<div class="row boxed-grey-humanprac"  >
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<div class="wow bounceInUp" data-wow-delay="0.2s">
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<div class="inner">
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<h5>Testing It Out</h5>
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<p class="subtitle explain"> We wanted to test whether our cartridge would work. Hence, we culture Shewanella oneidensis MR1 containing the lactate permease and lactate dehydrogenase operon and used it for our cartridge. The measurement was made both with the Arduino as well as a multimeter as we would like to have a comparison of any deviations from the multimeter. However, no significant deviations was  detected as the values reflected in the Arduino closely matches the value in the multimeter.
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<div align="center">
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/8/83/Me-testing.jpeg" width="720px" height="440px">
 
</div>
 
</div>
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<p style="text-align:center; font-size: 19px; color: black">
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Kean Hean making final adjustments to the reader. :)
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</p>
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<div align="center">
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/c/c5/12029054_10206580918214988_307882780_n.jpg" width="720px" height="550px">
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</div>
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<p style="text-align:center; font-size: 19px; color: black">
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Walla!! Shezensor showing initial readings. On the right side is the cartridge port.
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</p>
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<div align="center">
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/c/c0/12047209_10206580914694900_586161976_n.jpg" width="720px" height="550px">
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</div>
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<p style="text-align:center; font-size: 19px; color: black">
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Shezonsor and friend!! :)
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</p>
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</p>
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</div>
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</div>
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</div>
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<br>
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</div>
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</div>
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</section>
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<!--Activity4-->
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<div class="container">
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<div class="row boxed-grey-humanprac "  >
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<div class="wow bounceInUp" data-wow-delay="0.2s">
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<div class="inner">
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<h5>Results</h5>
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<p class="subtitle explain">From the data, we can see that there is an initial rise of the voltage to 679mV and starts to decline after 42mins. This suggests that our cartridge works and further development on the cartridge can be carried out. More specifically, the LacI protein can be conjugated into this strain as the promoter used for our operon is pLac. Hence, the induction of the sensor should be further investigated. However, this is not done in our project due to the time constraints.
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<div align="center">
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/1/18/Shezensor_result.png" width="720px" height="550px">
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</div>
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<p style="text-align:center; font-size: 19px; color: black">
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Shezensor output.
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</p>
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</p>
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</div>
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</div>
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</div>
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<br>
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</div>
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Latest revision as of 03:16, 19 September 2015

NTU SG iGEM 2015




Shezensor 1.0

The Concept

As MFCs have the potential to be used as a biosensor which is able to detect various chemicals and produce an electrical output. The ability to convert chemical to electrical signals gives MFCs an advantage over biosensors which rely on chemical outputs, i.e. fluorescence proteins as the electrical output allows on-field monitoring, automated data collection as well as WIFI communication.
For our project, our idea to make a portable device which allows users to test the cleanliness of water samples. Our device has two important components, the reader and the sensor. The reader is an arduino-controlled voltmeter and has a 16x2 LCD screen which provides a voltage reading to the user. While the sensor is a disposable cartridge which houses the MFC. Liquid samples can be loaded into the sensors and plugged into the reader to check if the voltage reading. After designing the cartridge, we carried out a proof-of-concept experiment to test the feasibility of our concept by first testing whether our disposable cartridge works.


Design

As mentioned in the concept, we will be using the Arduino board as the voltage reader, hence, the programming for the Arduino board has to be done so that it can be used along with a LCD display screen. On the reader, a cartridge port was made to allow connection of the anode and cathode to the reader.

For the cartridge, the materials we used were acrylic blocks and Booby helped carve the block outs as the machines are highly dangerous. The internal dimensions are 60 X 20 X 5mm, while the dimensions of our carbon cloth is 50 X 20mm. The area allowed for the proton exchange membrane is maximised to increased voltage output, with an area of 60mm2. When all the parts are carved out with the help of wonderful Bobby, we glued them together with a specialised acrylic glue and made sure that no leakage happens.

P.S. Our first prototype leaked, and we made another one. :P

The Blueprint.

The Catridge.

The Reader.


Testing It Out

We wanted to test whether our cartridge would work. Hence, we culture Shewanella oneidensis MR1 containing the lactate permease and lactate dehydrogenase operon and used it for our cartridge. The measurement was made both with the Arduino as well as a multimeter as we would like to have a comparison of any deviations from the multimeter. However, no significant deviations was detected as the values reflected in the Arduino closely matches the value in the multimeter.

Kean Hean making final adjustments to the reader. :)

Walla!! Shezensor showing initial readings. On the right side is the cartridge port.

Shezonsor and friend!! :)