Difference between revisions of "Team:Santa Clara/Safety"

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<h2>Safety in iGEM</h2>
 
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<div id="innerContent" class="container-fluid">
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h1>Safety</h1>
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<p>At Santa Clara University we have high standards health and safety for students, faculty and staff. We uphold the values strictly and strive to keep the university community safe. The Environment Health and Safety Department’s (EHS) mission is to:
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"support and advance the teaching, learning and research activities of the University through promotion of a safe and healthy campus environment by providing and coordinating programs and services that minimize safety, health, environmental and regulatory risks to the SCU community. The Environment, Health and Safety Department work to:
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<h4>Safe Project Design</h4>
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<ul>
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<li>Provide a safe campus learning and work environment</li>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Ensure a process of regulatory compliance</li>
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<li>Minimize future potential liabilities</li>
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<li>Protect and enhance SCU's reputation"</li>
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</ul>
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To learn about the EHS Department at Santa Clara University please visit their <a href="http://university-operations.scu.edu/ehs/">website</a>.
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</p>
  
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<h3>Our Project</h3>
 
<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Biological Chassis: <i>Escherichia Coli</i></li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<ul>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Sterile Equipment was used when dealing with <i>E. Coli</i></li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<li>All disposables in contact with <i>E. Coli</i> were sterilized via autoclaving to kill all live remnants.</li>
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</ul>
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<li>Sterile Technique</li>
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<ul>
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<li>All glassware, tips, consumables, and culture medias were autoclaved before use to prevent contamination.</li>
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<ul>
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<li>Autoclave is only used by students who are autoclaved trained by Biology Prep Room Staff.</li>
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<li>Proper Protective gear must be worn when using autoclave to prevent harm to user.</li>
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</ul>
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</ul>
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<li>Part Use</li>
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<ul>
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<li>All parts used/created are inherently/work in bacteria to no harm to the host or to humans if contamination occurs.</li>
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</ul>
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<li>In Gel Dye Substitution</li>
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<ul>
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<li>Traditional DNA visualization on agarose gels is accomplished through Ethidium Bromide. Ethidium bromide is a carcinogenic chemical and needs to be disposed of in special manners.</li>
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<li>Instead a synthetic in gel dye was used to visualize DNA instead of Ethidium bromide. This allowed for no special waste or care.</li>
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<li>Gel Red can be purchased <a href="https://biotium.com/product/gelredtm-nucleic-acid-gel-stain-10000x-in-water/">here</a></li>
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</ul>
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<li>Everyday Lab practices</li>
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<ul>
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<li>Lab coats and gloves used when working in lab.</li>
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</ul>
 
</ul>
 
</ul>
  
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<h4>Safe Lab Work</h4>
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    <a href="https://2015.igem.org/Team:Santa_Clara/Protocols">
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      <span class="glyphicon glyphicon-arrow-left"></span> Protocols
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    <a href="https://2015.igem.org/Team:Santa_Clara/Practices">
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      Practices <span class="glyphicon glyphicon-arrow-right"></span>
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    </a>
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  </li>
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</ul>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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</div>
  
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<h4>Safe Shipment</h4>
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</html>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 03:21, 19 September 2015

Santa Clara Template for iGEM wiki site

Safety

At Santa Clara University we have high standards health and safety for students, faculty and staff. We uphold the values strictly and strive to keep the university community safe. The Environment Health and Safety Department’s (EHS) mission is to: "support and advance the teaching, learning and research activities of the University through promotion of a safe and healthy campus environment by providing and coordinating programs and services that minimize safety, health, environmental and regulatory risks to the SCU community. The Environment, Health and Safety Department work to:

  • Provide a safe campus learning and work environment
  • Ensure a process of regulatory compliance
  • Minimize future potential liabilities
  • Protect and enhance SCU's reputation"
To learn about the EHS Department at Santa Clara University please visit their website.

Our Project

  • Biological Chassis: Escherichia Coli
    • Sterile Equipment was used when dealing with E. Coli
    • All disposables in contact with E. Coli were sterilized via autoclaving to kill all live remnants.
  • Sterile Technique
    • All glassware, tips, consumables, and culture medias were autoclaved before use to prevent contamination.
      • Autoclave is only used by students who are autoclaved trained by Biology Prep Room Staff.
      • Proper Protective gear must be worn when using autoclave to prevent harm to user.
  • Part Use
    • All parts used/created are inherently/work in bacteria to no harm to the host or to humans if contamination occurs.
  • In Gel Dye Substitution
    • Traditional DNA visualization on agarose gels is accomplished through Ethidium Bromide. Ethidium bromide is a carcinogenic chemical and needs to be disposed of in special manners.
    • Instead a synthetic in gel dye was used to visualize DNA instead of Ethidium bromide. This allowed for no special waste or care.
    • Gel Red can be purchased here
  • Everyday Lab practices
    • Lab coats and gloves used when working in lab.