Difference between revisions of "Team:BIT/Composite Part"

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<h2> Composite Parts</h2>
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<h1> Composite Parts</h1>
  
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<div class="highlightBox">
 
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<h4>Note</h4>
 
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
 
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</div>
 
  
 
<p>
 
<p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
        We really like this composite part, whose part name is "BBa_K1651000".
 +
        For it really make everyone begin to think carefully that what synthetic
 +
        can do for our life as well as how can it change our life.<br>
 +
        This year, we aim at detecting macromolecule by synthetic ways. Once we
 +
        decided to carry out our project, there are so many problems and considerations
 +
        that we must solve or think about. Nowadays, we already have many methods
 +
        to prevent us from the most serious disease. The most advanced techniques
 +
        will make confirmation in only several days or even several hours while
 +
        detecting Hepatitis B, AIDS and many other infectious disease. <br>
 +
        However, there are still wide areas, especially in the developing country,
 +
        which need some new kind of detecting methods for the device now widely used
 +
        is not portable enough. What's more, it is also not available with a low cost.
 +
 
 +
    </p>
 +
<br><br>
 +
 
 +
<p>
 +
        So all the considerations comes to one termination that is whether there
 +
        is a method, which has a low cost as well as portability.<br>
 +
        We are convinced that by optimizing the natural circuit existing in P. Aeruginosa.<br>
 +
        Like the real circuit, we use the LasIR and RhlIR system to construct a "sensor+amplifier"
 +
        in the E. Coli. With the small molecule PAI1 and PAI2 transformed plying between the sensor and
 +
        amplifier, the signal of the fluorescence will be greatly enhanced.<br>
 +
        This part named BBa_K1651000 is exactly the "amplifier" of the system, which can be significantly
 +
        activated by levels of small molecules (PAI1) can be satisfied.<br>
 +
        The following is our data coming from Laboratory for Bioseparation and Bioanalysis.
 +
            </p>
 +
    <div align="center"><img src="https://static.igem.org/mediawiki/2015/c/c1/Best_4.png" alt=""> </div>
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    <div align="center"><img src="https://static.igem.org/mediawiki/2015/7/7b/Best_1.png" alt=""> </div>
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    <div align="center"><img src="https://static.igem.org/mediawiki/2015/e/ed/Best_2.png" alt=""> </div>
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    <div align="center"><img src="https://static.igem.org/mediawiki/2015/6/63/Best_3.png" alt=""> </div>
 +
    <br>
 +
<p>
 +
        From the data above, it is obvious that the amplifier could be induced by trace of
 +
        PAI1 which is cataloged by the LasI promoted by the pBAD. So we have enough confidence to make
 +
        this part a powerful tool for any "sensor" which use pBAD as its promoter.  
 +
 
 
</p>
 
</p>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
  
  
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                    <h1>contact info</h1>
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                    <address>
 +
                        <p>Address: Beijing Institute of Technology, No. 5 South Zhong Guan Cun Street,
 +
Haidian Beijing 100081, P. R. China </p>
 +
 +
                        <p>Twitter : @igem_BIT</p>
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Latest revision as of 03:21, 19 September 2015

iGem_BIT

Composite Parts

We really like this composite part, whose part name is "BBa_K1651000". For it really make everyone begin to think carefully that what synthetic can do for our life as well as how can it change our life.
This year, we aim at detecting macromolecule by synthetic ways. Once we decided to carry out our project, there are so many problems and considerations that we must solve or think about. Nowadays, we already have many methods to prevent us from the most serious disease. The most advanced techniques will make confirmation in only several days or even several hours while detecting Hepatitis B, AIDS and many other infectious disease.
However, there are still wide areas, especially in the developing country, which need some new kind of detecting methods for the device now widely used is not portable enough. What's more, it is also not available with a low cost.



So all the considerations comes to one termination that is whether there is a method, which has a low cost as well as portability.
We are convinced that by optimizing the natural circuit existing in P. Aeruginosa.
Like the real circuit, we use the LasIR and RhlIR system to construct a "sensor+amplifier" in the E. Coli. With the small molecule PAI1 and PAI2 transformed plying between the sensor and amplifier, the signal of the fluorescence will be greatly enhanced.
This part named BBa_K1651000 is exactly the "amplifier" of the system, which can be significantly activated by levels of small molecules (PAI1) can be satisfied.
The following is our data coming from Laboratory for Bioseparation and Bioanalysis.


From the data above, it is obvious that the amplifier could be induced by trace of PAI1 which is cataloged by the LasI promoted by the pBAD. So we have enough confidence to make this part a powerful tool for any "sensor" which use pBAD as its promoter.

contact info

Address: Beijing Institute of Technology, No. 5 South Zhong Guan Cun Street, Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn

stay connected