Difference between revisions of "Team:Fudan/Description"

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<h2> Project Description </h2>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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DESCRIPTION
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<br /><p>We develop several linker based on former linker(BBa_K1486003). For part <a href="http://parts.igem.org/Part:BBa_ K1777017">BBa_K1777017</a>，We have optimized the codon for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. (Part <a href="http://parts.igem.org/Part:BBa_ K1777005">BBa_K1777005</a>, <a href="http://parts.igem.org/Part:BBa_ K1777018">BBa_K1777018</a> are also designed based on similar consideration)</p>
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<br /><p>Apart from that, we use beta-globin intron to provide SA and SD sequence in our circular RNA device(<a href="http://parts.igem.org/Part:BBa_ K1777001">BBa_K1777001</a>, <a href="http://parts.igem.org/Part:BBa_ K1777002">BBa_K1777002</a>, <a href="http://parts.igem.org/Part:BBa_ K1777003">BBa_K1777003</a>), which is inspired by the beta-globin intron parts（<a href="http://parts.igem.org/Part:BBa_ K404119">BBa_K404119</a>）. Beta-globin intron provides long flanking intron for circularizing exon and can increase the expression level. We use this character of Beta-globin intron to improve our circRNA expression level.</p>
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<br /><p>Apart from all the parts listed above, we have acRNA biobricks and a dozen of cyclizing protein biobrick.( BBa_K1777008, BBa_K1777009, BBa_K1777010, BBa_K1777011, BBa_K1777012, BBa_K1777013, BBa_K1777014,  BBa_K1777004, BBa_K1777005, BBa_K1777017 )</p>
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<ul>
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<li><a href="https://2015.igem.org/Team:Fudan">Fudan HOME</a></li>
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<li><a href="https://2015.igem.org/Team:Fudan/Achievement">ACHIEVEMENT</a> | <a href="https://2015.igem.org/Team:Fudan/Design">DESIGN</a> | <a href="https://2015.igem.org/Team:Fudan/Parts">PARTS</a> | <a href="https://2015.igem.org/Team:Fudan/Result">RESULT</a></li>
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<li><a href="https://2015.igem.org/Team:Fudan/Attributions">ATTRIBUTION</a> | <a href="https://2015.igem.org/Team:Fudan/Practices">PRACTICE</a> | <a href="https://2015.igem.org/Team:Fudan/Collaborations">COLLABORATION</a> | <a href="https://2015.igem.org/Team:Fudan/Notebook">NOTEBOOK</a></li>
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<li><CONTACT US: bertalanffy@163.com</li>
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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<h4>Advice on writing your Project Description</h4>
 
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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Latest revision as of 03:23, 19 September 2015

Description

DESCRIPTION

We develop several linker based on former linker(BBa_K1486003). For part BBa_K1777017，We have optimized the codon for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. (Part BBa_K1777005, BBa_K1777018 are also designed based on similar consideration)


Apart from that, we use beta-globin intron to provide SA and SD sequence in our circular RNA device(BBa_K1777001, BBa_K1777002, BBa_K1777003), which is inspired by the beta-globin intron parts（BBa_K404119）. Beta-globin intron provides long flanking intron for circularizing exon and can increase the expression level. We use this character of Beta-globin intron to improve our circRNA expression level.


Apart from all the parts listed above, we have acRNA biobricks and a dozen of cyclizing protein biobrick.( BBa_K1777008, BBa_K1777009, BBa_K1777010, BBa_K1777011, BBa_K1777012, BBa_K1777013, BBa_K1777014, BBa_K1777004, BBa_K1777005, BBa_K1777017 )