Difference between revisions of "Team:IIT Delhi/results"
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<br> <br> | <br> <br> | ||
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+ | <a href="http://parts.igem.org/Part:BBa_K1866006" target="_blank"> Part:BBa_K1866006</a> | ||
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+ | <br> | ||
+ | |||
+ | Promoter+RibosomeBindingSite+NrfA (in pSB1C3) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | The assimilatory Nitrite Reductase gene under the control of a strong Promoter Anderson J23104 with a Ribosome Binding Site was cloned onto the pSB1C3 plasmid backbone. This gene catalyses the conversion of Nitrite (NO2 -) to Ammonium (NH4 +). | ||
+ | |||
+ | <br><br> | ||
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+ | <a href="http://parts.igem.org/Part:BBa_K1866007" target="_blank"> Part:BBa_K1866007</a> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | NosZ+SuperYellowFluorescenceProtein (in pSB1C3) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | This part consists of NosZ gene and a sequence encoding for Super Yellow Fluorescence Protein downstream to it. | ||
+ | |||
+ | NosZ gene product catalyses the conversion of Nitrous Oxide (N2O) to molecular Nitrogen gas in a process called denitrification. | ||
+ | |||
+ | This part can be cloned under the control of a promoter of choice and its expression can be readily analyzed by checking for the expression of the yellow fluorescence protein. | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | <a href="http://parts.igem.org/Part:BBa_K1866008" target="_blank"> Part:BBa_K1866008</a> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | Promoter+RibosomeBindingSite+NrfA+SuperYellowFluorescenceProtein (in pSB1C3) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | This part has the Super Yellow Fluorescence Protein tagged to the NrfA gene product. | ||
+ | |||
+ | The Promoter+RBS+NrfA construct was obtained from our biobrick BBa_K1866000. The RBS+SYFP construct was procured from the biobrick BBa_K864101. | ||
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+ | <br><br> | ||
+ | |||
+ | <a href="http://parts.igem.org/Part:BBa_K1866009" target="_blank"> Part:BBa_K1866009</a> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | promoter+RibosomeBindingSite+Sqr (in pSB1C3) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | This part consists of the Sulfide Quinone Reductase Sqr gene under the control of a strong promoter with a Ribosome Binding Site. The Sqr gene catalyses the conversion of hydrogen Sulfide gas to elemental Sulfur. | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | <a href="http://parts.igem.org/Part:BBa_K1866010" target="_blank"> Part:BBa_K1866010</a> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | NosZ gene+SuperYellowFluorescenceProtein (in pSB1A3) | ||
+ | |||
+ | <br> | ||
+ | |||
+ | This part consists of NosZ gene and a sequence encoding for Super Yellow Fluorescence Protein downstream to it. | ||
+ | |||
+ | NosZ gene product catalyses the conversion of Nitrous Oxide (N2O) to molecular Nitrogen gas in a process called denitrification. | ||
+ | |||
+ | This part can be cloned under the control of a promoter of choice and its expression can be readily analyzed by checking for the expression of the yellow fluorescence protein. | ||
+ | |||
+ | <br><br> | ||
+ | |||
<FONT COLOR="blue"><b>Clone Confirmation Using Agarose Gel Electrophoresis</b> </FONT><br><br> | <FONT COLOR="blue"><b>Clone Confirmation Using Agarose Gel Electrophoresis</b> </FONT><br><br> | ||
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<table width="100%" height="100%" align="center" valign="center"> | <table width="100%" height="100%" align="center" valign="center"> | ||
<tr><td> | <tr><td> | ||
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/a/a0/Indophenol.png" /> <br><br> |
+ | <img src="https://static.igem.org/mediawiki/2015/d/d6/Indophenol_conc.png" /> | ||
</td></tr> | </td></tr> | ||
</table> | </table> | ||
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<table width="100%" height="100%" align="center" valign="center"> | <table width="100%" height="100%" align="center" valign="center"> | ||
<tr><td> | <tr><td> | ||
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/d/db/Phtest.png" /> <br><br> |
</td></tr> | </td></tr> | ||
</table> | </table> | ||
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<table width="100%" height="100%" align="center" valign="center"> | <table width="100%" height="100%" align="center" valign="center"> | ||
<tr><td> | <tr><td> | ||
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/5/50/Nesslers.png" /> <br><br> |
</td></tr> | </td></tr> | ||
</table> | </table> | ||
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</h2> | </h2> | ||
</td> <br><br> | </td> <br><br> | ||
− | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
<table> | <table> | ||
<tr id="attributions"> | <tr id="attributions"> | ||
+ | <!--- | ||
<td><img class="team" src="https://static.igem.org/mediawiki/2015/8/80/20150904_004012.jpg" height="200px" width="200px"></td> | <td><img class="team" src="https://static.igem.org/mediawiki/2015/8/80/20150904_004012.jpg" height="200px" width="200px"></td> | ||
+ | --> | ||
<td class="det"> | <td class="det"> | ||
<h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Acknowledgement</h1> | <h1 style="font-family:'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif;color:white;font-size:250%;padding-top:18px;">Acknowledgement</h1> | ||
<h2 style="font-family: 'Roboto', sans-serif;color:white;font-size:150%;vertical-align:text-top;"> | <h2 style="font-family: 'Roboto', sans-serif;color:white;font-size:150%;vertical-align:text-top;"> | ||
− | A few months back, it was a dream. Today, it’s a reality. The transition didn't come by our efforts alone. There | + | A few months back, it was a dream. Today, it’s a reality. The transition didn't come by our efforts alone. There were people who didn’t hesitate to spend their valuable time on us. And we certainly do owe a lot to them. We are grateful to our mentor Prof. Shaikh Ziauddin Ahammad, who selflessly supported us in unfavorable situation, for facilitating funding and allowing us access to UG lab. It was a fortunate coincidence that Dr. Stefan Oehler accepted role of primary trouble shooter for our molecular biology work, and happily answered our queries. We are grateful to him that he allowed us to leach his lab resources. We are also thankful to Prof. James Gomes, from the Kusuma School of Biological Sciences, for his valuable advice and moral support, and Prof. Biswajit Kundu for helpful conversations. |
− | + | <br><br> | |
− | We are | + | A special note is due to Prof. P.V.M Rao who went beyond his way to facilitate our work and for letting us the access to Workshop, IIT Delhi. We are thankful to Prof. Anurag Sharma, Dean, IIT Delhi for helping us sail administrative processes. Our team owes a lot to Anamika Singh (RNA 2 Lab), who provided constant support and help, no matter how late it was in the night. Also a thank you note to Garima Goyal (Protein Engineering Lab) for allowing us to access to her labs. We are also thankful to the Karan Varshney for helping us make crowd funding video. |
− | + | </h2> | |
− | + | ||
− | + | ||
− | We are also thankful to the | + | |
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<!-------------------------------footer stuff start---------------------------------------> | <!-------------------------------footer stuff start---------------------------------------> | ||
− | <footer style="height:20%;background-color:#1E4C7A;position:absolute;margin-top: | + | <footer style="height:20%;background-color:#1E4C7A;position:absolute;margin-top:5400px;width:100%;"> |
<div style="background-color:#1E4C7A;"> | <div style="background-color:#1E4C7A;"> |
Latest revision as of 03:24, 19 September 2015
Parts Submitted
Part:BBa_K1866000
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Criteria Fulfilled
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Acknowledgement
A few months back, it was a dream. Today, it’s a reality. The transition didn't come by our efforts alone. There were people who didn’t hesitate to spend their valuable time on us. And we certainly do owe a lot to them. We are grateful to our mentor Prof. Shaikh Ziauddin Ahammad, who selflessly supported us in unfavorable situation, for facilitating funding and allowing us access to UG lab. It was a fortunate coincidence that Dr. Stefan Oehler accepted role of primary trouble shooter for our molecular biology work, and happily answered our queries. We are grateful to him that he allowed us to leach his lab resources. We are also thankful to Prof. James Gomes, from the Kusuma School of Biological Sciences, for his valuable advice and moral support, and Prof. Biswajit Kundu for helpful conversations.
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