Difference between revisions of "Team:UNC-Chapel Hill/Parts"

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<tr><td  bgColor="#56A0D3"></td> <td colspan="3" width="975px" bgColor="#56A0D3" align="center"> <p style="color:white;font-size:20px">Selected Parts</p></td> <td  bgColor="#56A0D3"></td> </tr>
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<h2> Part Documentation</h2>
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<h3 style="color:#56A0D3;"> Part BBa_K1838000: MLC1</h3>
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<p>This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found downstream of the base promoter used for construction, BBa_J23119.</p>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
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<h4>Note</h4>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h4>Adding parts to the registry</h4>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h3 style="color:#56A0D3;"> Part BBa_K1838003: MLC4</h3>
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<p> This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found upstream of the base promoter used for construction, BBa_J23110</p>
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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<h3 style="color:#56A0D3;"> Part BBa_K1838005: MLC2 w/ Yellow Chromoprotein</h3>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a strong promoter when placed upstream of the base promoter sequence.
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h4>Part Table </h4>
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<h3 style="color:#56A0D3;"> Part BBa_K1838006: MLC3 w/ Yellow Chromoprotein</h3>
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<p> This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a weak promoter when placed downstream of the base promoter sequence.
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<groupparts>iGEM015 Example</groupparts>
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<h3 style="color:#56A0D3;"> Part BBa_K1838008: Glucose Inducible w/ Blue Chromoprotein Construct</h3>
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<p> This composite part features the glucose inducible promoter BBa_K861171 combined via 3A assembly with a blue chromoprotein+RBS part (BBa_K1073021).  This part is meant to be placed into the tri-color glucose detection composite part in order to contribute increasing levels of blue protein as glucose concentration rises.
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<center><groupparts>iGEM015 UNC-Chapel_Hill</groupparts></center>

Latest revision as of 03:28, 19 September 2015

Selected Parts

Part BBa_K1838000: MLC1

This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found downstream of the base promoter used for construction, BBa_J23119.

Part BBa_K1838003: MLC4

This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found upstream of the base promoter used for construction, BBa_J23110

Part BBa_K1838005: MLC2 w/ Yellow Chromoprotein

This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a strong promoter when placed upstream of the base promoter sequence.

Part BBa_K1838006: MLC3 w/ Yellow Chromoprotein

This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a weak promoter when placed downstream of the base promoter sequence.

Part BBa_K1838008: Glucose Inducible w/ Blue Chromoprotein Construct

This composite part features the glucose inducible promoter BBa_K861171 combined via 3A assembly with a blue chromoprotein+RBS part (BBa_K1073021). This part is meant to be placed into the tri-color glucose detection composite part in order to contribute increasing levels of blue protein as glucose concentration rises.

<groupparts>iGEM015 UNC-Chapel_Hill</groupparts>