Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

 
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{{Chalmers-Gothenburg}}
  
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<h2>BioBricks</h2>
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<h3>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h3>
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<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in <i>S.cerevisiae</i>.</p>
  
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<h3>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h3>
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<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
  
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<h3>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h3>
body {
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<p>Promoter to TPI1.</p>
background-color: #fff;
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}
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<h3>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h3>
#mainContainer { 
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<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.
width: 978px;
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Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
overflow:hidden;
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float:left;
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margin-left:20px;
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margin-bottom: 10px;
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background-color: #fff;
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border-bottom: 14px solid #565656;
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border-right: 2px solid #565656;
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border-left: 2px solid #565656;
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border-top: 2px solid #565656;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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}
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/* Creates the container for the menu */
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<h3>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h3>
#menuContainer  {
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<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
float:left;
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width: 134px;
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padding: 20px 0px;
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border-top: 14px solid #565656;
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background-color: #E8E8E9;
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}
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/* Creates the container for the content */
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#contentContainer {
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padding-top:20px;
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padding-right:10px;
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margin-bottom: 20px;
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width: 814px;
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padding-left: 20px;
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float: left;
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background-color: #fff;
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border-top: 14px solid #565656;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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}
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/*Set up height place holder for the banner*/
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#bannerContainer {
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height:200px;
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margin:auto;
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text-align:center;
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color: #24B694;
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}
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/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
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/*Change the styling of text for everything inside main container*/
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#mainContainer p {
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font-size: 13px;
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color: #000000;
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}
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/*This changes the color and font family */
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#contentContainer h1, h2, h3, h4, h5, h6 {
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color: #565656;
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border-bottom: none;
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font-weight: bold;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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margin-top:10px;
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}
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/*Style of the links - links are different inside the menu */
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#contentContainer a {
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font-weight: bold;
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color: #23b593;
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}
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  /* Styling links on hover- links are different inside the menu */
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#contentContainer a:hover {
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color: #59bf92;
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}
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/*Change the styling of tables */
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        #contentContainer table {
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    border: 1px solid #565656;
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    border-collapse: collapse;
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    width: 90%
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    margin: auto;
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    margin-bottom: 15px;
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    margin-top: 15px;
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    margin-right: 10px;
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    margin-left: 10px;
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    }
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/*Change the styling of table cells*/
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    #contentContainer  td {
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    padding: 10px;
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    border: 1px solid #565656;
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    border-collapse: collapse;
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    vertical-align: text-top;
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    }
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/*Change the styling of table headers */
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    #contentContainer th {
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    background-color: #E8E8E9;
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    padding: 10px;
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    border: 1px solid #565656;
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    border-collapse: collapse;
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    vertical-align: text-top;
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    }
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/*MENU STYLING */
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/*Styling for the links in the menu */
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#menuContainer a {
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color: #565656;
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text-decoration:none;
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font-weight: bold;
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}
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/* Sets the style for lists inside menuContainer  */
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#menuContainer ul {
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list-style: none;
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margin-left:0px;
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}
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/*Styles the list items to become menu buttons */
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#menuContainer ul li {
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text-align: center;
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display: block;
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width: 100%;
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height:30px;
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padding-top:10px;
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}
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/*For the menu buttons, changes the color when hovering*/
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#menuContainer li:hover {
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color:  #FFF;
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background-color: #24B694;
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}
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/*Submenus are not displayed as default*/
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#menuContainer li ul {
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display: none;
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padding-top:15px;
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margin-left: -19px;
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}
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/*Submenus are displayed when hovering the menu button */
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#menuContainer li:hover ul {
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/*display: inline-block; */
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display: block;
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position: absolute;
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float:right;
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margin-left: 134px;
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margin-top:-42px;
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}
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/*Style the submenu buttons*/
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#menuContainer li ul li{
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background-color: #59BF92;
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padding-left:20px;
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padding-right:20px;
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height:30px;
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padding-top:10px;
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margin-top:-2px;
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color: #565656;
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width: 150px;
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}
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/*CLASSES */
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/*Clear class for all the pages, adds spacing too*/
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.clear{
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clear:both;
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height: 10px;
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}
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/* highlight box for special messages */
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    .highlightBox {
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        width:500px;
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        margin:auto;
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        background-color: #E8E8E9;
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margin-bottom: 15px;
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margin-top: 15px;
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padding: 15px;
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padding-top: 5px;
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    }
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</style>
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<!-- Start of the template html elements. -->
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<div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
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<div id="bannerContainer">
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<br><br>
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<h2> Add a banner to your wiki! </h2>
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<p>You can make the image 980px  by  200px</p>
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<p> Remember to call the file: "<i>Team_Chalmers-Gothenburg_banner.jpg</i>" </p>
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</div>
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<div id="menuContainer">
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<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
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<ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg"><li>HOME</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Team"><li>TEAM
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<ul> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Students"><li>Students</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Supervisors"><li>Supervisors</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Gallery"><li>Gallery</li></a>
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project"><li>PROJECT
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<ul> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Theory"><li>Theory</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Constructs"><li>Constructs</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Protocols"><li>Protocols</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/OtherApplications"><li>Other applications</li></a>
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Achievements"><li>ACHIEVEMENTS
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<ul> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project Results"><li>Project Results</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/BioBricks"><li>BioBricks</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Medals"><li>Medals</li></a> 
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</ul>
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</li></a>
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<a href="#"><li>MODELING
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            <ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Detection"><li>Detection</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety Switch"><li>Safety Switch</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Simulation"><li>Simulation</li></a> 
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Practices"><li>HUMAN PRACTICES
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<ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/iGEMCommunity"><li>iGEM Community</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/NextGeneration"><li>Next Generation</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Society"><li>Society</li></a> 
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</ul></a></li>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Notebook"><li>NOTEBOOK
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<ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Schedule"><li>Schedule</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety"><li>SAFETY
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<ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/LabSafety"><li>Lab Safety</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Ethics"><li>Ethics</li></a> 
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Entrepreneurship"><li>THANKS
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<ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Sponsors"><li>Sponsors</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Acknowledgements"><li>Acknowledgements</li></a> 
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</ul>
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</li></a>
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</ul>
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</div>
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<div id="contentContainer"> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
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</html>
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<h1>BioBricks</h1>
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<h2>1. BBa_K1321000: pTEF1-pSUC2</h2>
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<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations. </p>
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<h2>2. BBa_K1321001: STE2MAM2</h2>
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<p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway. </p>
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<h2>3. BBa_K1321002: RecA</h2>
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<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.
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Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
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</p>
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<h2>4. BBa_K1321004: pTPI1</h2>
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<p>promoter to TPI1. </p>
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<h2>5. BBa_K1321005: Ssb</h2>
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<p>From Deinococcus Radiodurans
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Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
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</p>
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Latest revision as of 03:33, 19 September 2015

BioBricks

1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2

Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in S.cerevisiae.

2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1

Promoter to TPI1.

4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA

Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB

Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.